ES2219324T3 - Determinacion de formas biologicamente activas de enzimas proteoliticas. - Google Patents

Determinacion de formas biologicamente activas de enzimas proteoliticas.

Info

Publication number
ES2219324T3
ES2219324T3 ES00914107T ES00914107T ES2219324T3 ES 2219324 T3 ES2219324 T3 ES 2219324T3 ES 00914107 T ES00914107 T ES 00914107T ES 00914107 T ES00914107 T ES 00914107T ES 2219324 T3 ES2219324 T3 ES 2219324T3
Authority
ES
Spain
Prior art keywords
thrombin
substrate
procedure
proteolytic enzyme
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES00914107T
Other languages
English (en)
Spanish (es)
Inventor
Hendrik Coenraad Hemker
Robert Jan Wagenvoord
Manoj Ramjee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Synapse BV
Original Assignee
Synapse BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synapse BV filed Critical Synapse BV
Application granted granted Critical
Publication of ES2219324T3 publication Critical patent/ES2219324T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10—Anilides
    • C12Q2337/12—Para-Nitroanilides p-NA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/20—Coumarin derivatives
    • C12Q2337/22—7-Amino-4-methylcoumarin, i.e. AMC, MCA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/76—Assays involving albumins other than in routine use for blocking surfaces or for anchoring haptens during immunisation
    • G01N2333/77—Ovalbumin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/81—Protease inhibitors
    • G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Neurosurgery (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
ES00914107T 1999-03-04 2000-03-03 Determinacion de formas biologicamente activas de enzimas proteoliticas. Expired - Lifetime ES2219324T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP99200627 1999-03-04
EP99200627 1999-03-04
US15724099P 1999-10-01 1999-10-01
US157240P 1999-10-01

Publications (1)

Publication Number Publication Date
ES2219324T3 true ES2219324T3 (es) 2004-12-01

Family

ID=44350544

Family Applications (1)

Application Number Title Priority Date Filing Date
ES00914107T Expired - Lifetime ES2219324T3 (es) 1999-03-04 2000-03-03 Determinacion de formas biologicamente activas de enzimas proteoliticas.

Country Status (9)

Country Link
EP (1) EP1159448B1 (https=)
JP (1) JP4721519B2 (https=)
AT (1) ATE264401T1 (https=)
AU (1) AU3553900A (https=)
DE (1) DE60009879T2 (https=)
DK (1) DK1159448T3 (https=)
ES (1) ES2219324T3 (https=)
PT (1) PT1159448E (https=)
WO (1) WO2000052199A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE50212934D1 (de) * 2001-01-26 2008-12-04 Evotec Ag Verfahren und mittel zur detektion enzymatischer spalt- und verknüpfungsreaktionen
ATE368229T1 (de) * 2002-05-01 2007-08-15 Synapse Bv Diagnostischer test zur bestimmung der konzentration einer transienten proteolytischen aktivität in zusammengesetzten biologischen medien
DE102005008066A1 (de) 2005-02-22 2006-08-24 Haemosys Gmbh Verfahren zur Bestimmung der Gesamtgerinnungsaktivität einer Blut- oder Plasmaprobe
DE102006035899A1 (de) * 2006-07-31 2008-02-07 Dade Behring Marburg Gmbh Polysacharid-Peptid-Konjugate zur Verwendung als Thrombinsubstrate
US8343730B2 (en) 2006-11-02 2013-01-01 Thrombinoscope B.V. Method for determining the course of proteolytic activity
EP2206063A1 (en) 2007-10-16 2010-07-14 Koninklijke Philips Electronics N.V. Estimation of diagnostic markers
RU2518247C2 (ru) * 2011-07-26 2014-06-10 Общество с ограниченной ответственностью "ГемаКор Лабс" (ООО "ГемаКор Лабс") Способ определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе, устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе
EP3363912B1 (en) 2017-02-17 2020-10-28 Synapse B.V. Self-calibrated assay for determining proteolytic enzyme generation, dedicated means and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2363854C3 (de) * 1973-12-21 1980-01-03 Behringwerke Ag, 3550 Marburg Substrat zur Proteinasebestimmung sowie dessen Herstellung
US4897444A (en) * 1985-05-31 1990-01-30 The Research Foundation Of The State University Of New York Immobilized fluorogenic substrates for enzymes; and processes for their preparation
US5073487A (en) * 1989-01-30 1991-12-17 Athens Research And Technology, Inc. Rapid assay for functional human α1 -proteinase inhibitor
DE69615339T2 (de) * 1995-01-10 2002-07-04 Hemker, Hendrik Coenraad Verfahren zum nachweis des endogenen thrombin-potentials und thrombin substrate zur verwendung in diesen verfahren

Also Published As

Publication number Publication date
ATE264401T1 (de) 2004-04-15
JP4721519B2 (ja) 2011-07-13
DK1159448T3 (da) 2004-08-09
EP1159448A1 (en) 2001-12-05
DE60009879T2 (de) 2005-03-31
WO2000052199A1 (en) 2000-09-08
PT1159448E (pt) 2004-08-31
AU3553900A (en) 2000-09-21
DE60009879D1 (de) 2004-05-19
JP2002537820A (ja) 2002-11-12
EP1159448B1 (en) 2004-04-14

Similar Documents

Publication Publication Date Title
JP5916367B2 (ja) 多数の凝固プロテアーゼを同時に決定するための方法
Gailani et al. Factor XII-independent activation of factor XI in plasma: effects of sulfatides on tissue factor-induced coagulation
US10465230B2 (en) Chemiluminescence-based haemostasis assay
ES2291661T3 (es) Prueba de diagnostico para la determinacion de la concentracion de actividad proteolitica transitoria en medios biologicos compuestos.
Bick Disseminated intravascular coagulation and related syndromes
ES2329672T3 (es) Ensayo hematologico y kit.
ES2219324T3 (es) Determinacion de formas biologicamente activas de enzimas proteoliticas.
Huseby et al. Synthetic Oligopeptide Substrates Their Diagnostic Application in Blood Coagulation, Fibrinolysis, and Other Pathologic States
EP2177625B1 (en) Blood coagulation assays
CA3116456A1 (en) Novel chemiluminescent substrates for factor xa
ES2357112T3 (es) Ensayo de hemostasia.
Gallimore et al. Studies on plasma antiplasmin activity using a new plasmin specific chromogenic tripeptide substrate
Bagoly et al. Down-regulation of activated factor XIII by polymorphonuclear granulocyte proteases within fibrin clot
JP2002537820A5 (https=)
Bennett Blood coagulation and coagulation tests
Meddahi et al. Standard measurement of clot-bound thrombin by using a chromogenic substrate for thrombin
Mann et al. Normal coagulation
Triplett et al. New methods in coagulation
US7691641B2 (en) Polysaccharide-peptide conjugates for use as thrombin substrates
ES2729800T3 (es) Ensayo de hemostasis basado en la quimioluminiscencia
Jesty Blood coagulation
WO1993011260A1 (en) Method for measuring fibrinolytic capacity within whole human plasma
Musgrave et al. The use of synthetic substrates in the coagulation laboratory
Berliner ESR and fluorescence studies of thrombin active site conformation
Hamasaki Sheshadri Narayanan