PT1159448E - Determinacao de formas biologicamente activas de enzimas proteoliticas - Google Patents
Determinacao de formas biologicamente activas de enzimas proteoliticasInfo
- Publication number
- PT1159448E PT1159448E PT00914107T PT00914107T PT1159448E PT 1159448 E PT1159448 E PT 1159448E PT 00914107 T PT00914107 T PT 00914107T PT 00914107 T PT00914107 T PT 00914107T PT 1159448 E PT1159448 E PT 1159448E
- Authority
- PT
- Portugal
- Prior art keywords
- substrate
- proteolytic enzyme
- molecules
- group
- activated
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 title 1
- 230000002797 proteolythic effect Effects 0.000 title 1
- 108091005804 Peptidases Proteins 0.000 abstract 4
- 102000035195 Peptidases Human genes 0.000 abstract 4
- 239000000758 substrate Substances 0.000 abstract 4
- 102100033312 Alpha-2-macroglobulin Human genes 0.000 abstract 2
- 108090000190 Thrombin Proteins 0.000 abstract 2
- 229960004072 thrombin Drugs 0.000 abstract 2
- 101710187168 Alpha-2-macroglobulin Proteins 0.000 abstract 1
- 101710136034 Alpha-2-macroglobulin homolog Proteins 0.000 abstract 1
- 108010039209 Blood Coagulation Factors Proteins 0.000 abstract 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 abstract 1
- 108010058846 Ovalbumin Proteins 0.000 abstract 1
- 108010015078 Pregnancy-Associated alpha 2-Macroglobulins Proteins 0.000 abstract 1
- 239000013060 biological fluid Substances 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 239000003114 blood coagulation factor Substances 0.000 abstract 1
- 230000004154 complement system Effects 0.000 abstract 1
- 239000003527 fibrinolytic agent Substances 0.000 abstract 1
- 230000003480 fibrinolytic effect Effects 0.000 abstract 1
- 150000004676 glycans Chemical class 0.000 abstract 1
- 229940092253 ovalbumin Drugs 0.000 abstract 1
- 229920001282 polysaccharide Polymers 0.000 abstract 1
- 239000005017 polysaccharide Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 229920001059 synthetic polymer Polymers 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/20—Coumarin derivatives
- C12Q2337/22—7-Amino-4-methylcoumarin, i.e. AMC, MCA
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/76—Assays involving albumins other than in routine use for blocking surfaces or for anchoring haptens during immunisation
- G01N2333/77—Ovalbumin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/81—Protease inhibitors
- G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Neurosurgery (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP99200627 | 1999-03-04 | ||
| US15724099P | 1999-10-01 | 1999-10-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT1159448E true PT1159448E (pt) | 2004-08-31 |
Family
ID=44350544
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT00914107T PT1159448E (pt) | 1999-03-04 | 2000-03-03 | Determinacao de formas biologicamente activas de enzimas proteoliticas |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1159448B1 (https=) |
| JP (1) | JP4721519B2 (https=) |
| AT (1) | ATE264401T1 (https=) |
| AU (1) | AU3553900A (https=) |
| DE (1) | DE60009879T2 (https=) |
| DK (1) | DK1159448T3 (https=) |
| ES (1) | ES2219324T3 (https=) |
| PT (1) | PT1159448E (https=) |
| WO (1) | WO2000052199A1 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE50212934D1 (de) * | 2001-01-26 | 2008-12-04 | Evotec Ag | Verfahren und mittel zur detektion enzymatischer spalt- und verknüpfungsreaktionen |
| ATE368229T1 (de) * | 2002-05-01 | 2007-08-15 | Synapse Bv | Diagnostischer test zur bestimmung der konzentration einer transienten proteolytischen aktivität in zusammengesetzten biologischen medien |
| DE102005008066A1 (de) | 2005-02-22 | 2006-08-24 | Haemosys Gmbh | Verfahren zur Bestimmung der Gesamtgerinnungsaktivität einer Blut- oder Plasmaprobe |
| DE102006035899A1 (de) * | 2006-07-31 | 2008-02-07 | Dade Behring Marburg Gmbh | Polysacharid-Peptid-Konjugate zur Verwendung als Thrombinsubstrate |
| US8343730B2 (en) | 2006-11-02 | 2013-01-01 | Thrombinoscope B.V. | Method for determining the course of proteolytic activity |
| EP2206063A1 (en) | 2007-10-16 | 2010-07-14 | Koninklijke Philips Electronics N.V. | Estimation of diagnostic markers |
| RU2518247C2 (ru) * | 2011-07-26 | 2014-06-10 | Общество с ограниченной ответственностью "ГемаКор Лабс" (ООО "ГемаКор Лабс") | Способ определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе, устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе |
| EP3363912B1 (en) | 2017-02-17 | 2020-10-28 | Synapse B.V. | Self-calibrated assay for determining proteolytic enzyme generation, dedicated means and uses thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2363854C3 (de) * | 1973-12-21 | 1980-01-03 | Behringwerke Ag, 3550 Marburg | Substrat zur Proteinasebestimmung sowie dessen Herstellung |
| US4897444A (en) * | 1985-05-31 | 1990-01-30 | The Research Foundation Of The State University Of New York | Immobilized fluorogenic substrates for enzymes; and processes for their preparation |
| US5073487A (en) * | 1989-01-30 | 1991-12-17 | Athens Research And Technology, Inc. | Rapid assay for functional human α1 -proteinase inhibitor |
| DE69615339T2 (de) * | 1995-01-10 | 2002-07-04 | Hemker, Hendrik Coenraad | Verfahren zum nachweis des endogenen thrombin-potentials und thrombin substrate zur verwendung in diesen verfahren |
-
2000
- 2000-03-03 WO PCT/EP2000/001912 patent/WO2000052199A1/en not_active Ceased
- 2000-03-03 JP JP2000602809A patent/JP4721519B2/ja not_active Expired - Lifetime
- 2000-03-03 DK DK00914107T patent/DK1159448T3/da active
- 2000-03-03 PT PT00914107T patent/PT1159448E/pt unknown
- 2000-03-03 ES ES00914107T patent/ES2219324T3/es not_active Expired - Lifetime
- 2000-03-03 AU AU35539/00A patent/AU3553900A/en not_active Abandoned
- 2000-03-03 AT AT00914107T patent/ATE264401T1/de active
- 2000-03-03 EP EP00914107A patent/EP1159448B1/en not_active Expired - Lifetime
- 2000-03-03 DE DE60009879T patent/DE60009879T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ATE264401T1 (de) | 2004-04-15 |
| JP4721519B2 (ja) | 2011-07-13 |
| DK1159448T3 (da) | 2004-08-09 |
| EP1159448A1 (en) | 2001-12-05 |
| ES2219324T3 (es) | 2004-12-01 |
| DE60009879T2 (de) | 2005-03-31 |
| WO2000052199A1 (en) | 2000-09-08 |
| AU3553900A (en) | 2000-09-21 |
| DE60009879D1 (de) | 2004-05-19 |
| JP2002537820A (ja) | 2002-11-12 |
| EP1159448B1 (en) | 2004-04-14 |
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