GB1442796A - Method for purification of physiologically active substance using enzyme-enzyme inhibitor system - Google Patents
Method for purification of physiologically active substance using enzyme-enzyme inhibitor systemInfo
- Publication number
- GB1442796A GB1442796A GB5868773A GB5868773A GB1442796A GB 1442796 A GB1442796 A GB 1442796A GB 5868773 A GB5868773 A GB 5868773A GB 5868773 A GB5868773 A GB 5868773A GB 1442796 A GB1442796 A GB 1442796A
- Authority
- GB
- United Kingdom
- Prior art keywords
- enzyme
- inhibitor
- insolubilized
- proteolytic
- enzymes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6445—Kallikreins (3.4.21.34; 3.4.21.35)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
1442796 Isolating and purifying proteolytic enzymes and inhibitors ONO PHARMACEUTICAL CO Ltd 18 Dec 1973 [19 Dec 1972] 58687/73 Heading C3H A method of isolating and purifying a physiologically active substance, defined as meaning either proteolytic enzymes which take part in the production-consumption of kinin or an inhibitor which is proteinaceous in nature and inhibits the activity of the above proteolytic enzyme, contained in mammalian tissue or body fluid comprises the steps of passing an extract of said tissue or a diluted solution of said body fluid through a combination of columns packed with an insolubilized naturally occurring proteolytic enzyme and an insolubilized naturally occurring proteolytic enzyme inhibitor corresponding to the enzyme, respectively, in a continuous manner to adsorb the corresponding enzyme inhibitors present in the extract or diluted solution on to the insolubilized enzyme and the corresponding enzymes present in the extract or diluted solution on to the insolubilized enzyme inhibitor, respectively, and eluting separately the adsorbed enzyme inhibitors and enzymes from the columns to obtain fractions containing the physiologically active substances. The proteolytic enzyme may be kallikrein, plasmin, thrombin, trypsin, or chymotrypsin and the proteolytic enzyme inhibitor may be the corresponding inhibitor to these enzymes. Preferably the insolubilized enzyme/inhibitor system comprises the proteolytic enzyme inhibitor bound to an insoluble agarose, dextran or polyacrylamide carrier and the proteolytic enzyme chemically bound to an insoluble agarose, dextran or polyacrylamide carrier.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12739672A JPS5325030B2 (en) | 1972-12-19 | 1972-12-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1442796A true GB1442796A (en) | 1976-07-14 |
Family
ID=14958946
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB5868773A Expired GB1442796A (en) | 1972-12-19 | 1973-12-18 | Method for purification of physiologically active substance using enzyme-enzyme inhibitor system |
Country Status (4)
Country | Link |
---|---|
JP (1) | JPS5325030B2 (en) |
DE (1) | DE2363201B2 (en) |
FR (1) | FR2210620B1 (en) |
GB (1) | GB1442796A (en) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT943553B (en) * | 1971-06-25 | 1973-04-10 | A Ciferri | PROCEDURE FOR MODIFYING SOME PHYSICAL-MECHANICAL CHARACTERISTICS OF POLYMER MATERIALS CONTAINING POLAR GROUPS |
US4030977A (en) * | 1972-12-19 | 1977-06-21 | Ono Pharmaceutical Co., Ltd. | Method for purification of physiologically active substance using enzyme-enzyme inhibitor system |
DE2612479A1 (en) * | 1975-04-18 | 1976-10-21 | Behringwerke Ag | PROCEDURE FOR PREGNANCY-SPECIFIC PREGNANCY-SPECIFIC BETA LOW 1-GLYCOPROTEIN ENrichment |
JPS5462312A (en) * | 1977-10-28 | 1979-05-19 | Tokyo Zouki Kagaku Kk | Purifying of kariclein |
DE3034043A1 (en) * | 1980-09-10 | 1982-04-22 | Boehringer Mannheim Gmbh, 6800 Mannheim | METHOD FOR SELECTIVE SEPARATION OF ENDOPROTEASES |
DE3103257A1 (en) * | 1981-01-31 | 1982-08-26 | Bayer Ag, 5090 Leverkusen | METHOD FOR PRODUCING THE HIGH PURITY ENZYME KALLIKREIN FROM PIG PANCREAS EXTRACTS |
AU538170B2 (en) * | 1982-09-15 | 1984-08-02 | Owens-Illinois Glass Container Inc. | Modified protein to mould an enzyme type molecule |
AU539729B2 (en) * | 1982-09-16 | 1984-10-11 | Owens-Illinois Glass Container Inc. | Protein moulding to form semi-synthetic enzymes |
AU538942B2 (en) * | 1982-09-16 | 1984-09-06 | Owens-Illinois Glass Container Inc. | Protein moulding to make enzyme-type action and immobilization |
JPS59109172A (en) * | 1982-12-13 | 1984-06-23 | Ss Pharmaceut Co Ltd | Purification of kallikrein |
JPS61247749A (en) * | 1985-04-26 | 1986-11-05 | Asahi Chem Ind Co Ltd | Acrylonitrile polymer containing metal or metallic compound |
BE1006312A3 (en) * | 1991-11-29 | 1994-07-19 | Univ Catholique Louvain | Selection process for recombinant microorganisms with their area at least one molecule enzymatic activity. |
-
1972
- 1972-12-19 JP JP12739672A patent/JPS5325030B2/ja not_active Expired
-
1973
- 1973-12-18 GB GB5868773A patent/GB1442796A/en not_active Expired
- 1973-12-19 FR FR7345585A patent/FR2210620B1/fr not_active Expired
- 1973-12-19 DE DE19732363201 patent/DE2363201B2/en not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
FR2210620B1 (en) | 1978-02-10 |
DE2363201B2 (en) | 1980-07-31 |
FR2210620A1 (en) | 1974-07-12 |
DE2363201A1 (en) | 1974-07-04 |
JPS5325030B2 (en) | 1978-07-24 |
JPS504227A (en) | 1975-01-17 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed | ||
PCNP | Patent ceased through non-payment of renewal fee |