ES2188632T3 - Metodo para la produccion de tgf-b. - Google Patents

Metodo para la produccion de tgf-b.

Info

Publication number
ES2188632T3
ES2188632T3 ES95119910T ES95119910T ES2188632T3 ES 2188632 T3 ES2188632 T3 ES 2188632T3 ES 95119910 T ES95119910 T ES 95119910T ES 95119910 T ES95119910 T ES 95119910T ES 2188632 T3 ES2188632 T3 ES 2188632T3
Authority
ES
Spain
Prior art keywords
tgf
reactor
cation exchange
production
chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95119910T
Other languages
English (en)
Inventor
Erno Pungaor Jr
Eirik Nestaas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Berlex Laboratories Inc
Original Assignee
Berlex Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berlex Laboratories Inc filed Critical Berlex Laboratories Inc
Application granted granted Critical
Publication of ES2188632T3 publication Critical patent/ES2188632T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/495—Transforming growth factor [TGF]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Sustainable Development (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Steroid Compounds (AREA)

Abstract

LA TRANSFORMACION DEL FACTOR DEL CRECIMIENTO BE (TGF- BE}) SE PRODUCE EN CANTIDADES RELATIVAMENTE GRANDES Y CON UNA PUREZA RELATIVAMENTE ALTA MEDIANTE FERMENTACION EN UN REACTOR DE MICROPORTADOR DE PERFUSION. EL MEDIO ACONDICIONADO DEL REACTOR SE TRATA PRIMERO PARA SUMINISTRAR LA FORMA ACTIVA DEL TGF-{BE} Y SUBSECUENTEMENTE SE PURIFICA MEDIANTE CROMATOGRAFIA DE INTERCAMBIO DE CATIONES CONTINUANDO CON UNA CROMATOGRAFIA DE INTERACCION HIDROFOBICA. OPCIONALMENTE, LOS ACIDOS NUCLEICOS COMPLEJADOS CON EL TGF-{BE} PUEDEN ELIMINARSE MIENTRAS QUE LA PROTEINA SE UNE A LA RESINA DE INTERCAMBIO DE CATIONES. LAS COMPOSICIONES DE TGF-{BE}1 PURIFICADO TIENEN UNA ACTIVIDAD ESPECIFICA POR ENCIMA DE 10{SUP,7} U/ML.
ES95119910T 1989-09-11 1990-09-10 Metodo para la produccion de tgf-b. Expired - Lifetime ES2188632T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/405,695 US5043431A (en) 1989-09-11 1989-09-11 Method and apparatus for the production of TGF-β

Publications (1)

Publication Number Publication Date
ES2188632T3 true ES2188632T3 (es) 2003-07-01

Family

ID=23604819

Family Applications (2)

Application Number Title Priority Date Filing Date
ES90914805T Expired - Lifetime ES2093031T3 (es) 1989-09-11 1990-09-10 Metodo para purificar el factor de crecimiento transformante (tgf).
ES95119910T Expired - Lifetime ES2188632T3 (es) 1989-09-11 1990-09-10 Metodo para la produccion de tgf-b.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES90914805T Expired - Lifetime ES2093031T3 (es) 1989-09-11 1990-09-10 Metodo para purificar el factor de crecimiento transformante (tgf).

Country Status (10)

Country Link
US (2) US5043431A (es)
EP (4) EP0722951B1 (es)
JP (1) JPH05500898A (es)
AT (2) ATE140927T1 (es)
AU (1) AU6504490A (es)
CA (1) CA2066576A1 (es)
DE (2) DE69027995T2 (es)
DK (2) DK0722951T3 (es)
ES (2) ES2093031T3 (es)
WO (1) WO1991004267A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5221734A (en) * 1987-10-01 1993-06-22 Ciba-Geigy Corporation Process for preparing a polypeptide growth factor for milk
US5043431A (en) * 1989-09-11 1991-08-27 Codon Method and apparatus for the production of TGF-β
US5206023A (en) * 1991-01-31 1993-04-27 Robert F. Shaw Method and compositions for the treatment and repair of defects or lesions in cartilage
US5270300A (en) * 1991-09-06 1993-12-14 Robert Francis Shaw Methods and compositions for the treatment and repair of defects or lesions in cartilage or bone
JP3386498B2 (ja) * 1992-11-06 2003-03-17 森永製菓株式会社 ヒトＴＧＦ−βの精製法
US5331095A (en) * 1993-04-12 1994-07-19 Scios Nova Inc. Process for purification of basic fibroblast growth factor
WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
DE19918409A1 (de) * 1999-04-22 2000-10-26 Forschungszentrum Juelich Gmbh Bioreaktor umfassend einen Medienablauf und einen Rührer sowie Rührer und Medienablauf
FR2827290B1 (fr) * 2001-07-13 2004-07-09 Pierre Jouan Biotechnologies Sa Procede d'obtention d'une fraction proteique enrichie en tgf-beta sous forme activee, fraction proteique et applications therapeutiques
US6849394B2 (en) * 2002-02-21 2005-02-01 Minitube Of America Compositions comprising reproductive cell media and methods for using such compositions
EP1728852A1 (en) * 2005-05-30 2006-12-06 Chiron Behring GmbH & Co. KG Fermenter system for biotechnical processes
CN102171360A (zh) * 2008-10-06 2011-08-31 弗·哈夫曼-拉罗切有限公司 小型振荡瓶培养
US10428130B2 (en) 2015-03-17 2019-10-01 Istituto Biochimico Italiano Giovanni Lorenzini S.P.A. Purification of bone morphogenetic proteins (BMPs)
GB201509193D0 (en) * 2015-05-28 2015-07-15 Ge Healthcare Bio Sciences Ab Method and system for cell cultivation
US9617191B1 (en) * 2016-06-09 2017-04-11 Xianggen Wu Bioreactor system and method

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5630925A (en) * 1979-08-21 1981-03-28 Sumitomo Chem Co Ltd Purification of human growth hormone
US4296205A (en) * 1980-02-22 1981-10-20 Verma Dharmvir S Cell culture and continuous dialysis flask and method
US4427580A (en) * 1982-09-01 1984-01-24 Cornell Research Foundation, Inc. Method for separation and recovery of proteins and nucleic acids from nucleoproteins using water destructuring salts
US4444760A (en) * 1983-06-17 1984-04-24 Merck & Co., Inc. Purification and characterization of a protein fibroblast growth factor
US4897464A (en) * 1985-04-17 1990-01-30 President And Fellows Of Harvard College Purified protein having angiogenic activity and methods of preparation
US4751078A (en) * 1985-07-03 1988-06-14 Nagabhushan Tattanahalli L Process for the purification of gamma interferon
US4894439A (en) * 1986-05-22 1990-01-16 Cetus Corporation N-terminal derivatives of tumor necrosis factor purified by microporous PTFE membranes
AU612617B2 (en) * 1986-11-17 1991-07-18 Sandoz Ag Production and use of a novel t-cell suppressor factor
US4931548A (en) * 1987-01-30 1990-06-05 Techne Corporation Heterodimer form of transforming growth factor-beta
EP0309111A3 (en) * 1987-09-03 1990-04-18 Invitron Corporation A process for obtaining higher yields of unaggregated tissue plasminogen activator from cell culture
EP0323842A3 (en) * 1988-01-07 1990-04-25 Otsuka Pharmaceutical Co., Ltd. Method of purification of transforming growth factor-beta
EP0338716A3 (en) * 1988-04-21 1990-04-11 Berlex Laboratories, Inc. Method and system for the production of non-degraded proteins from mammalian cells
US5043431A (en) * 1989-09-11 1991-08-27 Codon Method and apparatus for the production of TGF-β

Also Published As

Publication number Publication date
EP1132469A2 (en) 2001-09-12
EP1132469A3 (en) 2001-11-28
EP0491849A4 (en) 1993-04-07
EP0722951B1 (en) 2003-01-15
JPH05500898A (ja) 1993-02-25
WO1991004267A1 (en) 1991-04-04
EP0491849B1 (en) 1996-07-31
DK0722951T3 (da) 2003-03-24
ES2093031T3 (es) 1996-12-16
US5864021A (en) 1999-01-26
EP0491849A1 (en) 1992-07-01
DE69027995T2 (de) 1997-02-27
DE69027995D1 (de) 1996-09-05
DE69034037T2 (de) 2003-08-28
EP1104807A2 (en) 2001-06-06
DK0491849T3 (da) 1996-12-30
EP0722951A3 (en) 1996-10-23
DE69034037D1 (de) 2003-02-20
AU6504490A (en) 1991-04-18
ATE140927T1 (de) 1996-08-15
EP0722951A2 (en) 1996-07-24
ATE231165T1 (de) 2003-02-15
CA2066576A1 (en) 1991-03-12
US5043431A (en) 1991-08-27

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