JPS5618591A - Production of substance capable of differentiation and multiplication of stem cells of human granulocyte - Google Patents
Production of substance capable of differentiation and multiplication of stem cells of human granulocyteInfo
- Publication number
- JPS5618591A JPS5618591A JP9235579A JP9235579A JPS5618591A JP S5618591 A JPS5618591 A JP S5618591A JP 9235579 A JP9235579 A JP 9235579A JP 9235579 A JP9235579 A JP 9235579A JP S5618591 A JPS5618591 A JP S5618591A
- Authority
- JP
- Japan
- Prior art keywords
- multiplication
- separated
- stem cells
- human
- glycoprotein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
PURPOSE: Monocytes and macrophages separated from human venous blood are cultured in a medium containing a protein which is separated from human urine and capable of multiplying granulocytes in vivo, thus producing a substance having activity of promoting differentiation and multiplication of stem cells.
CONSTITUTION: Monocytes and macrophages separated from human venous blood are cultured in a medium for tissue culture containing a glycoprotein which has been separated from human urine and can multiply granulocytes in vivo to produce the effective component, which is collected from the medium, thus producing the substance capable of promoting differentiation and multiplication of stem cells of granulocyte. The glycoprotein is obtained by adding diluted acid or alkali to human urine to adjust the pH to 6W9, removing impurities in the urine by centrifugation, adsorbing the supernatant on silica gel eluting the purified glycoprotein with an aqueous alkali. The resultant liquid product is used directly for injection or powdery product is dissolved in sterilized water and the solution is injected.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9235579A JPS5618591A (en) | 1979-07-20 | 1979-07-20 | Production of substance capable of differentiation and multiplication of stem cells of human granulocyte |
US06/169,107 US4342828A (en) | 1979-07-20 | 1980-07-15 | Method for producing substance capable of stimulating differentiation and proliferation of human granulopoietic stem cells |
GB8023347A GB2058081B (en) | 1979-07-20 | 1980-07-17 | Method of producing a substance for curing human granulocytopoenia |
CA356,422A CA1128881A (en) | 1979-07-20 | 1980-07-17 | Method for producing substance capable of stimulating differentiation and proliferation of human granulopoietic stem cells |
DE19803027105 DE3027105A1 (en) | 1979-07-20 | 1980-07-17 | METHOD FOR PRODUCING A FACTOR STIMULATING THE PROLIFERATION AND DIFFERENTIATION OF HUMAN GRANULOPOETIC STEM CELLS |
CH550580A CH644520A5 (en) | 1979-07-20 | 1980-07-18 | METHOD FOR PRODUCING A FACTOR STIMULATING PROLIFERATION AND DIFFERENTIATION OF HUMAN GRANULOPOETIC STEM CELLS. |
SE8005256A SE451850B (en) | 1979-07-20 | 1980-07-18 | SET TO MAKE A GLYCOPROTEIN WITH KNOWN ABILITY TO STIMULATE EDUCATION AND DIFFERENTIZATION OF HUMAN GRANULOCYTES |
FR8015923A FR2461500A1 (en) | 1979-07-20 | 1980-07-18 | PROCESS FOR PRODUCING A SUBSTANCE CAPABLE OF STIMULATING THE PROLIFERATION AND DIFFERENTIATION OF HUMAN GRANULOPOIETIC STEM CELLS |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9235579A JPS5618591A (en) | 1979-07-20 | 1979-07-20 | Production of substance capable of differentiation and multiplication of stem cells of human granulocyte |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5618591A true JPS5618591A (en) | 1981-02-21 |
JPS6237605B2 JPS6237605B2 (en) | 1987-08-13 |
Family
ID=14052084
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9235579A Granted JPS5618591A (en) | 1979-07-20 | 1979-07-20 | Production of substance capable of differentiation and multiplication of stem cells of human granulocyte |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5618591A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62174026A (en) * | 1985-10-04 | 1987-07-30 | Chugai Pharmaceut Co Ltd | Remedy for leukpenia |
-
1979
- 1979-07-20 JP JP9235579A patent/JPS5618591A/en active Granted
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62174026A (en) * | 1985-10-04 | 1987-07-30 | Chugai Pharmaceut Co Ltd | Remedy for leukpenia |
Also Published As
Publication number | Publication date |
---|---|
JPS6237605B2 (en) | 1987-08-13 |
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