JPS5639794A - Preparation of interferon - Google Patents
Preparation of interferonInfo
- Publication number
- JPS5639794A JPS5639794A JP11322579A JP11322579A JPS5639794A JP S5639794 A JPS5639794 A JP S5639794A JP 11322579 A JP11322579 A JP 11322579A JP 11322579 A JP11322579 A JP 11322579A JP S5639794 A JPS5639794 A JP S5639794A
- Authority
- JP
- Japan
- Prior art keywords
- interferon
- cluture
- incubated
- fluid
- human lymphoblastic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE: To mass-produce interferon without the stimulation of an interferon inducer, by incubating a human lymphoblastic cell from prolonged infection with measles virus in a nutrient culture medium, and collecting interferon from the culture fluid.
CONSTITUTION: An incubated human lymphoblastic cell is inoculated with an attenuated variant of measles virus in an amount of 10W102 infection value per 103 cells, and incubated in a nutrient cluture medium containing 10% a bovine fetal serum to give a stable human lymphoblastic cell from prolonged infection with measles virus. The human lymphoblastic cell from prolonged infectio with meales virus is incubated in a culture medium usually used for the tissue culture of an animal, and the interferon is separated and collected from the resultant cluture fluid by the well-known separation means, e.g. separation of the cell from th cluture fluid through centrifugation, treatment with a surfactant, and salting out. The addition of a serum, e.g. the bovine fetal serum, to the nutrient culture medium improves the production of the interferon.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11322579A JPS5639794A (en) | 1979-09-04 | 1979-09-04 | Preparation of interferon |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11322579A JPS5639794A (en) | 1979-09-04 | 1979-09-04 | Preparation of interferon |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5639794A true JPS5639794A (en) | 1981-04-15 |
Family
ID=14606730
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11322579A Pending JPS5639794A (en) | 1979-09-04 | 1979-09-04 | Preparation of interferon |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5639794A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS57188524A (en) * | 1981-05-15 | 1982-11-19 | Thomae Gmbh Dr K | Interferon |
JPS61135598A (en) * | 1984-12-04 | 1986-06-23 | Green Cross Corp:The | Production of interferon-alpha |
-
1979
- 1979-09-04 JP JP11322579A patent/JPS5639794A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS57188524A (en) * | 1981-05-15 | 1982-11-19 | Thomae Gmbh Dr K | Interferon |
JPS61135598A (en) * | 1984-12-04 | 1986-06-23 | Green Cross Corp:The | Production of interferon-alpha |
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