JPS5765179A - Cell culture - Google Patents
Cell cultureInfo
- Publication number
- JPS5765179A JPS5765179A JP55139853A JP13985380A JPS5765179A JP S5765179 A JPS5765179 A JP S5765179A JP 55139853 A JP55139853 A JP 55139853A JP 13985380 A JP13985380 A JP 13985380A JP S5765179 A JPS5765179 A JP S5765179A
- Authority
- JP
- Japan
- Prior art keywords
- animal
- chlorella
- subculture
- extract
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: The addition of the extract from microscopic algae such as Chlorella to the culture medium enables the subculture of animal cells by no use of animal serum.
CONSTITUTION: Microscopic algae such as Chlorella, Scenedesmus or Spirulina are extracted with hot water or an aqueous solvent and the extract is molecularly fractionated to collect a fraction containing saccharides and proteins. In the cultivation of animal cells such as cancer cells, the above fraction is added to a usual base medium by 0.3W400γ/ml to have the effect of reducing the proportion of animal serum from the usual level of 10% to 1% and permit the subculture.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55139853A JPS603827B2 (en) | 1980-10-08 | 1980-10-08 | Cell culture method |
AU75269/81A AU546565B2 (en) | 1980-10-08 | 1981-09-16 | Method of tissue culture |
CA000386671A CA1165707A (en) | 1980-10-08 | 1981-09-25 | Method of tissue culture |
DE8181304612T DE3171242D1 (en) | 1980-10-06 | 1981-10-05 | Cell culture method |
AT81304612T ATE14143T1 (en) | 1980-10-06 | 1981-10-05 | CELL CULTURE PROCEDURE. |
EP19810304612 EP0049632B1 (en) | 1980-10-06 | 1981-10-05 | Cell culture method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55139853A JPS603827B2 (en) | 1980-10-08 | 1980-10-08 | Cell culture method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5765179A true JPS5765179A (en) | 1982-04-20 |
JPS603827B2 JPS603827B2 (en) | 1985-01-30 |
Family
ID=15255053
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP55139853A Expired JPS603827B2 (en) | 1980-10-06 | 1980-10-08 | Cell culture method |
Country Status (3)
Country | Link |
---|---|
JP (1) | JPS603827B2 (en) |
AU (1) | AU546565B2 (en) |
CA (1) | CA1165707A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021066113A1 (en) | 2019-10-01 | 2021-04-08 | 学校法人東京女子医科大学 | Production method for composition for cell culturing, composition for cell culturing obtained by same, and cell culturing method using same |
WO2022270598A1 (en) | 2021-06-23 | 2022-12-29 | 学校法人東京女子医科大学 | Method for producing composition for culturing animal cells, composition for culturing animal cells obtained by said method, and method for culturing animal cells using said composition for culturing animal cells |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62174024A (en) * | 1986-01-24 | 1987-07-30 | Kurorera Kogyo Kk | Remedy for neurophilic dysfunction |
JP2520681B2 (en) * | 1986-08-04 | 1996-07-31 | ザ ユニバーシティ オブ ニュー サウス ウェールズ | Biosynthetic human growth hormone products |
-
1980
- 1980-10-08 JP JP55139853A patent/JPS603827B2/en not_active Expired
-
1981
- 1981-09-16 AU AU75269/81A patent/AU546565B2/en not_active Ceased
- 1981-09-25 CA CA000386671A patent/CA1165707A/en not_active Expired
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021066113A1 (en) | 2019-10-01 | 2021-04-08 | 学校法人東京女子医科大学 | Production method for composition for cell culturing, composition for cell culturing obtained by same, and cell culturing method using same |
WO2022270598A1 (en) | 2021-06-23 | 2022-12-29 | 学校法人東京女子医科大学 | Method for producing composition for culturing animal cells, composition for culturing animal cells obtained by said method, and method for culturing animal cells using said composition for culturing animal cells |
Also Published As
Publication number | Publication date |
---|---|
CA1165707A (en) | 1984-04-17 |
AU546565B2 (en) | 1985-09-05 |
JPS603827B2 (en) | 1985-01-30 |
AU7526981A (en) | 1982-04-22 |
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