JPS5765179A - Cell culture - Google Patents

Cell culture

Info

Publication number
JPS5765179A
JPS5765179A JP55139853A JP13985380A JPS5765179A JP S5765179 A JPS5765179 A JP S5765179A JP 55139853 A JP55139853 A JP 55139853A JP 13985380 A JP13985380 A JP 13985380A JP S5765179 A JPS5765179 A JP S5765179A
Authority
JP
Japan
Prior art keywords
animal
chlorella
subculture
extract
cell culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP55139853A
Other languages
Japanese (ja)
Other versions
JPS603827B2 (en
Inventor
Hajime Katsuta
Fusako Takaoka
Masao Okuda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DOKKYO GAKUEN EDUCAT FOUND
DOTSUKIYOU GAKUEN
KURORERA KOGYO KK
Chlorella Industry Co Ltd
Original Assignee
DOKKYO GAKUEN EDUCAT FOUND
DOTSUKIYOU GAKUEN
KURORERA KOGYO KK
Chlorella Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DOKKYO GAKUEN EDUCAT FOUND, DOTSUKIYOU GAKUEN, KURORERA KOGYO KK, Chlorella Industry Co Ltd filed Critical DOKKYO GAKUEN EDUCAT FOUND
Priority to JP55139853A priority Critical patent/JPS603827B2/en
Priority to AU75269/81A priority patent/AU546565B2/en
Priority to CA000386671A priority patent/CA1165707A/en
Priority to DE8181304612T priority patent/DE3171242D1/en
Priority to AT81304612T priority patent/ATE14143T1/en
Priority to EP19810304612 priority patent/EP0049632B1/en
Publication of JPS5765179A publication Critical patent/JPS5765179A/en
Publication of JPS603827B2 publication Critical patent/JPS603827B2/en
Expired legal-status Critical Current

Links

Abstract

PURPOSE: The addition of the extract from microscopic algae such as Chlorella to the culture medium enables the subculture of animal cells by no use of animal serum.
CONSTITUTION: Microscopic algae such as Chlorella, Scenedesmus or Spirulina are extracted with hot water or an aqueous solvent and the extract is molecularly fractionated to collect a fraction containing saccharides and proteins. In the cultivation of animal cells such as cancer cells, the above fraction is added to a usual base medium by 0.3W400γ/ml to have the effect of reducing the proportion of animal serum from the usual level of 10% to 1% and permit the subculture.
COPYRIGHT: (C)1982,JPO&Japio
JP55139853A 1980-10-06 1980-10-08 Cell culture method Expired JPS603827B2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP55139853A JPS603827B2 (en) 1980-10-08 1980-10-08 Cell culture method
AU75269/81A AU546565B2 (en) 1980-10-08 1981-09-16 Method of tissue culture
CA000386671A CA1165707A (en) 1980-10-08 1981-09-25 Method of tissue culture
DE8181304612T DE3171242D1 (en) 1980-10-06 1981-10-05 Cell culture method
AT81304612T ATE14143T1 (en) 1980-10-06 1981-10-05 CELL CULTURE PROCEDURE.
EP19810304612 EP0049632B1 (en) 1980-10-06 1981-10-05 Cell culture method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55139853A JPS603827B2 (en) 1980-10-08 1980-10-08 Cell culture method

Publications (2)

Publication Number Publication Date
JPS5765179A true JPS5765179A (en) 1982-04-20
JPS603827B2 JPS603827B2 (en) 1985-01-30

Family

ID=15255053

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55139853A Expired JPS603827B2 (en) 1980-10-06 1980-10-08 Cell culture method

Country Status (3)

Country Link
JP (1) JPS603827B2 (en)
AU (1) AU546565B2 (en)
CA (1) CA1165707A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021066113A1 (en) 2019-10-01 2021-04-08 学校法人東京女子医科大学 Production method for composition for cell culturing, composition for cell culturing obtained by same, and cell culturing method using same
WO2022270598A1 (en) 2021-06-23 2022-12-29 学校法人東京女子医科大学 Method for producing composition for culturing animal cells, composition for culturing animal cells obtained by said method, and method for culturing animal cells using said composition for culturing animal cells

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62174024A (en) * 1986-01-24 1987-07-30 Kurorera Kogyo Kk Remedy for neurophilic dysfunction
JP2520681B2 (en) * 1986-08-04 1996-07-31 ザ ユニバーシティ オブ ニュー サウス ウェールズ Biosynthetic human growth hormone products

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021066113A1 (en) 2019-10-01 2021-04-08 学校法人東京女子医科大学 Production method for composition for cell culturing, composition for cell culturing obtained by same, and cell culturing method using same
WO2022270598A1 (en) 2021-06-23 2022-12-29 学校法人東京女子医科大学 Method for producing composition for culturing animal cells, composition for culturing animal cells obtained by said method, and method for culturing animal cells using said composition for culturing animal cells

Also Published As

Publication number Publication date
CA1165707A (en) 1984-04-17
AU546565B2 (en) 1985-09-05
JPS603827B2 (en) 1985-01-30
AU7526981A (en) 1982-04-22

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