BG50942A3 - Method for production of chymopapain - Google Patents
Method for production of chymopapainInfo
- Publication number
- BG50942A3 BG50942A3 BG84338A BG8433888A BG50942A3 BG 50942 A3 BG50942 A3 BG 50942A3 BG 84338 A BG84338 A BG 84338A BG 8433888 A BG8433888 A BG 8433888A BG 50942 A3 BG50942 A3 BG 50942A3
- Authority
- BG
- Bulgaria
- Prior art keywords
- chymopapain
- sephadex
- derived
- production
- cystein
- Prior art date
Links
- 108090001069 Chymopapain Proteins 0.000 title abstract 3
- ISWQCIVKKSOKNN-UHFFFAOYSA-L Tiron Chemical compound [Na+].[Na+].OC1=CC(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C1O ISWQCIVKKSOKNN-UHFFFAOYSA-L 0.000 title abstract 3
- 229960002976 chymopapain Drugs 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title 1
- 229920005654 Sephadex Polymers 0.000 abstract 2
- 239000012507 Sephadex™ Substances 0.000 abstract 2
- 206010019909 Hernia Diseases 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 abstract 1
- 239000008351 acetate buffer Substances 0.000 abstract 1
- 239000003638 chemical reducing agent Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000005227 gel permeation chromatography Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 abstract 1
- 239000004289 sodium hydrogen sulphite Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
This method is used in medicine for extracting medical agents for the treatment of inter-vertebra disc hernia. The product is highly active and is derived in a simple way : the raw chymopapain is dissolved in water containing a phosphate or acetate buffer, with a pH between 5 and 7, in the presence of a reducer, such as cystein or sodium bisulphite. The derived pure solution is subjected to gel chromatography in a column in proportion between the length and diameter in the 5:1 and 50:1 range and containing sephadex G-50 or sephadex G-25, after which the chymopapain fractions are gathered and lyophilised.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BG84338A BG50942A3 (en) | 1986-12-08 | 1988-06-01 | Method for production of chymopapain |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU865090A HU196624B (en) | 1986-12-08 | 1986-12-08 | Process for producing chymopapain with high specific activity |
| BG84338A BG50942A3 (en) | 1986-12-08 | 1988-06-01 | Method for production of chymopapain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BG50942A3 true BG50942A3 (en) | 1992-12-30 |
Family
ID=10969645
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BG84338A BG50942A3 (en) | 1986-12-08 | 1988-06-01 | Method for production of chymopapain |
Country Status (5)
| Country | Link |
|---|---|
| BG (1) | BG50942A3 (en) |
| CS (1) | CS271346B2 (en) |
| CU (1) | CU22158A3 (en) |
| DD (1) | DD270723A5 (en) |
| HU (1) | HU196624B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8909836D0 (en) * | 1989-04-28 | 1989-06-14 | Boots Co Plc | Therapeutic agent |
| CN1320111C (en) * | 2004-08-09 | 2007-06-06 | 王美岭 | Process for preparing papain |
-
1986
- 1986-12-08 HU HU865090A patent/HU196624B/en not_active IP Right Cessation
-
1988
- 1988-05-18 CU CU8870A patent/CU22158A3/en unknown
- 1988-06-01 DD DD31628488A patent/DD270723A5/en not_active IP Right Cessation
- 1988-06-01 BG BG84338A patent/BG50942A3/en unknown
- 1988-06-01 CS CS883773A patent/CS271346B2/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HU196624B (en) | 1988-12-28 |
| CS377388A2 (en) | 1989-12-13 |
| CU22158A3 (en) | 1994-01-31 |
| DD270723A5 (en) | 1989-08-09 |
| CS271346B2 (en) | 1990-09-12 |
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