FR2393006A1 - Procede de purification de l'urokinase - Google Patents
Procede de purification de l'urokinaseInfo
- Publication number
- FR2393006A1 FR2393006A1 FR7816192A FR7816192A FR2393006A1 FR 2393006 A1 FR2393006 A1 FR 2393006A1 FR 7816192 A FR7816192 A FR 7816192A FR 7816192 A FR7816192 A FR 7816192A FR 2393006 A1 FR2393006 A1 FR 2393006A1
- Authority
- FR
- France
- Prior art keywords
- urokinase
- solution containing
- crude
- urea
- purified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 title abstract 10
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 title abstract 10
- 229960005356 urokinase Drugs 0.000 title abstract 10
- 238000000746 purification Methods 0.000 title 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 2
- 239000004202 carbamide Substances 0.000 abstract 2
- 150000001768 cations Chemical class 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 101710196208 Fibrinolytic enzyme Proteins 0.000 abstract 1
- 102000013566 Plasminogen Human genes 0.000 abstract 1
- 108010051456 Plasminogen Proteins 0.000 abstract 1
- 208000007536 Thrombosis Diseases 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 230000003327 cancerostatic effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 229940012957 plasmin Drugs 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 125000000542 sulfonic acid group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
L'urokinase est purifiée par la mise en contact d'une solution contenant de l'urokinase brute avec un échangeur de cations fortement acide qui contient un groupe acide sulfonique et est réglé sur le type NH 4 **+ afin d'adsorber l'urokinase sur cet échangeur de cations, puis par l'extraction de l'urokinase à partir de l'échangeur par élution. On ajoute au préalable de l'urée à la solution contenant l'urokinase brute dans une proportion telle que la concentration finale d'urée dans la solution se situe entre 3 et 8 M. Ce procédé est propre à fournir une urokinase purifiée qui présente une activité pratiquement constante et très spécifique, à partir d'une solution contenant de l'urokinase brute, avec un rendement élevé, quel que soit le degré de pureté de l'urokinase brute. L'urokinase est un enzyme qui est capable de produire la plasmine, enzyme fibrinolytique, à partir de plasminogène, et l'on sait que cette substance est non seulement utile pour le traitement de diverses sortes de thrombose, mais qu'elle est également capable de renforcer remarquablement le pouvoir des agents carcinostatiques lorsqu'elle est utilisée en association avec ces agents.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP52065975A JPS5935B2 (ja) | 1977-06-03 | 1977-06-03 | ウロキナ−ゼの精製法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2393006A1 true FR2393006A1 (fr) | 1978-12-29 |
| FR2393006B1 FR2393006B1 (fr) | 1981-05-08 |
Family
ID=13302500
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR7816192A Granted FR2393006A1 (fr) | 1977-06-03 | 1978-05-31 | Procede de purification de l'urokinase |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US4189350A (fr) |
| JP (1) | JPS5935B2 (fr) |
| CA (1) | CA1102263A (fr) |
| DE (1) | DE2823353A1 (fr) |
| DK (1) | DK147266C (fr) |
| FR (1) | FR2393006A1 (fr) |
| GB (1) | GB1556652A (fr) |
| IT (1) | IT7849692A0 (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1988000615A1 (fr) * | 1986-07-16 | 1988-01-28 | Celltech Limited | Procede de purification d'un activateur de plasminogene |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2121050B (en) * | 1979-07-05 | 1986-03-26 | Genentech Inc | Preparation of functional human urokinase proteins |
| US5112755A (en) * | 1982-04-15 | 1992-05-12 | Genentech, Inc. | Preparation of functional human urokinase proteins |
| JPS60183797U (ja) * | 1984-05-17 | 1985-12-05 | 三菱重工業株式会社 | フオ−クリフトの信号伝送装置 |
| JPS6186400A (ja) * | 1984-09-29 | 1986-05-01 | 新明和工業株式会社 | 高所作業車の操作情報制御装置 |
| DE3439980A1 (de) * | 1984-11-02 | 1986-05-07 | Behringwerke Ag, 3550 Marburg | Verfahren zur reinigung sowie pasteurisierung von urokinase |
| JPS61115492A (ja) * | 1984-11-07 | 1986-06-03 | Green Cross Corp:The | ウロキナ−ゼの精製方法 |
| KR100419448B1 (ko) * | 2001-03-09 | 2004-02-19 | 주)녹십자 | 고정화 유로키나아제 칼럼의 재생방법 |
| US11982045B2 (en) * | 2022-05-11 | 2024-05-14 | Imam Abdulrahman Bin Faisal University | System and methods for recycling heat and water in a steam press machine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1289809B (de) * | 1965-10-01 | 1969-02-27 | Green Cross Corp | Verfahren zur Gewinnung von Urokinase fuer Injektionszwecke |
| JPS5212984A (en) * | 1975-07-22 | 1977-01-31 | Kanebo Ltd | Purification of urokinase |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3957582A (en) * | 1974-11-20 | 1976-05-18 | Abbott Laboratories | Purification of urokinase |
| JPS51123885A (en) * | 1975-04-18 | 1976-10-28 | Hitachi Chem Co Ltd | Process for purifying urokinase |
-
1977
- 1977-06-03 JP JP52065975A patent/JPS5935B2/ja not_active Expired
-
1978
- 1978-05-29 DE DE19782823353 patent/DE2823353A1/de not_active Withdrawn
- 1978-05-31 FR FR7816192A patent/FR2393006A1/fr active Granted
- 1978-05-31 GB GB24852/78A patent/GB1556652A/en not_active Expired
- 1978-06-01 CA CA304,573A patent/CA1102263A/fr not_active Expired
- 1978-06-01 US US05/911,475 patent/US4189350A/en not_active Expired - Lifetime
- 1978-06-02 IT IT7849692A patent/IT7849692A0/it unknown
- 1978-06-02 DK DK248678A patent/DK147266C/da not_active IP Right Cessation
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1289809B (de) * | 1965-10-01 | 1969-02-27 | Green Cross Corp | Verfahren zur Gewinnung von Urokinase fuer Injektionszwecke |
| JPS5212984A (en) * | 1975-07-22 | 1977-01-31 | Kanebo Ltd | Purification of urokinase |
Non-Patent Citations (2)
| Title |
|---|
| CA1975 * |
| CA1977 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1988000615A1 (fr) * | 1986-07-16 | 1988-01-28 | Celltech Limited | Procede de purification d'un activateur de plasminogene |
Also Published As
| Publication number | Publication date |
|---|---|
| IT7849692A0 (it) | 1978-06-02 |
| US4189350A (en) | 1980-02-19 |
| JPS5935B2 (ja) | 1984-01-05 |
| GB1556652A (en) | 1979-11-28 |
| JPS542396A (en) | 1979-01-09 |
| DK147266B (da) | 1984-05-28 |
| FR2393006B1 (fr) | 1981-05-08 |
| DK248678A (da) | 1978-12-04 |
| DK147266C (da) | 1984-12-10 |
| DE2823353A1 (de) | 1978-12-21 |
| CA1102263A (fr) | 1981-06-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |