ES2181007T3 - Ensayo de interaccion de ligandos con transferencia de energia fluorescente sobre una pelicula lipidica. - Google Patents

Ensayo de interaccion de ligandos con transferencia de energia fluorescente sobre una pelicula lipidica.

Info

Publication number
ES2181007T3
ES2181007T3 ES97928295T ES97928295T ES2181007T3 ES 2181007 T3 ES2181007 T3 ES 2181007T3 ES 97928295 T ES97928295 T ES 97928295T ES 97928295 T ES97928295 T ES 97928295T ES 2181007 T3 ES2181007 T3 ES 2181007T3
Authority
ES
Spain
Prior art keywords
ligands
interaction
test
energy transfer
lipid film
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97928295T
Other languages
English (en)
Inventor
Kari Keinanen
Marja-Leena Laukkanen
Hans Soderlund
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VTT Technical Research Centre of Finland Ltd
Original Assignee
VTT Technical Research Centre of Finland Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VTT Technical Research Centre of Finland Ltd filed Critical VTT Technical Research Centre of Finland Ltd
Application granted granted Critical
Publication of ES2181007T3 publication Critical patent/ES2181007T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/819—Multifunctional antigen or antibody
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/829—Liposomes, e.g. encapsulation

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Luminescent Compositions (AREA)
  • Steroid Compounds (AREA)

Abstract

ESTA INVENCION SE RELACIONA CON UN PROCEDIMIENTO DE INMUNOVALORACION BASANDOSE EN LA FLUORESCENCIA PARA DETECTAR UN ANALITO O PARA MEDIR SU CONCENTRACION EN UNA MUESTRA BIOLOGICA. EL PROCEDIMIENTO SE BASA EN LA CAPACIDAD DE UN ANALITO MULTIVALENTE DE INDUCIR LA AGREGACION DE LAS MOLECULAS RECEPTORAS ETIQUETADAS CON UN FLUOROFORO. ESTAS MOLECULAS ESTAN ANCLADAS A ESTE Y SE PUEDEN DESPLAZAR LIBREMENTE EN UNA MEMBRANA DE LIPIDOS Y, POR ELLO, ORIGINAN CAMBIOS EN LA FLUORESCENCIA.
ES97928295T 1996-06-28 1997-06-30 Ensayo de interaccion de ligandos con transferencia de energia fluorescente sobre una pelicula lipidica. Expired - Lifetime ES2181007T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI962686A FI102922B1 (fi) 1996-06-28 1996-06-28 Fluoresenssiin perustuva immunomääritysmenetelmä

Publications (1)

Publication Number Publication Date
ES2181007T3 true ES2181007T3 (es) 2003-02-16

Family

ID=8546307

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97928295T Expired - Lifetime ES2181007T3 (es) 1996-06-28 1997-06-30 Ensayo de interaccion de ligandos con transferencia de energia fluorescente sobre una pelicula lipidica.

Country Status (14)

Country Link
US (1) US6235535B1 (es)
EP (1) EP0932830B1 (es)
JP (1) JP3436374B2 (es)
AT (1) ATE226323T1 (es)
AU (1) AU722353B2 (es)
CA (1) CA2257941A1 (es)
DE (1) DE69716454T2 (es)
DK (1) DK0932830T3 (es)
ES (1) ES2181007T3 (es)
FI (1) FI102922B1 (es)
NO (1) NO986043L (es)
NZ (1) NZ333472A (es)
PT (1) PT932830E (es)
WO (1) WO1998000714A1 (es)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9801120D0 (en) 1998-01-21 1998-03-18 Secr Defence Detection system
WO1999067640A1 (en) * 1998-06-22 1999-12-29 The Regents Of The University Of California Triggered optical biosensor
AU2604200A (en) * 1999-01-08 2000-07-24 Panacos Pharmaceuticals, Inc. Methods of eliciting broadly neutralizing antibodies targeting hiv-1 gp41
US6627396B1 (en) * 1999-10-28 2003-09-30 The Regents Of The University Of California Influenza sensor
US6984494B2 (en) * 2000-08-15 2006-01-10 Genentech, Inc. Analytical method
AT410601B (de) * 2000-12-29 2003-06-25 Hoffmann La Roche Sensor zur lumineszenz-optischen bestimmung eines analyten sowie reagens, das nach dem fret-prinzip arbeitet
WO2002064837A1 (en) * 2001-02-14 2002-08-22 University Of Maryland, Baltimore Radiative decay engineering
US20020192721A1 (en) * 2001-03-28 2002-12-19 Engeneos, Inc. Modular molecular clasps and uses thereof
CA2448719A1 (en) * 2001-06-09 2002-12-19 Glsynthesis Inc. Lipid structures and uses thereof
US20030059811A1 (en) * 2001-06-14 2003-03-27 Hakim Djaballah Methods of screening for ligands of target molecules
US6713272B2 (en) * 2001-09-19 2004-03-30 The United States Of America As Represented By The Secretary Of The Navy Attachment of biomolecules to hydrophobic surfaces
WO2004011642A1 (en) * 2002-03-21 2004-02-05 The Regents Of The University Of California Generic membrane anchoring system
AU2003221788A1 (en) * 2002-04-30 2003-11-17 University Of Maryland, Baltimore Fluorescence sensing
US20040146959A1 (en) * 2002-09-09 2004-07-29 Graham Ronald J. Fluorescent enzyme assay methods and compositions
WO2004025262A2 (en) * 2002-09-11 2004-03-25 Synamem Corporation Membrane-based assays
WO2004042404A1 (en) * 2002-11-07 2004-05-21 Erasmus Universiteit Rotterdam Fret probes and methods for detecting interacting molecules
US20040171175A1 (en) * 2003-02-28 2004-09-02 Swanson Basil I. Process for conjugating biomolecules to hydrophobic membrane-incorporated molecules
US8741577B2 (en) * 2003-04-07 2014-06-03 Bio-Rad Laboratories Inc. Surface immobilised multilayer structure of vesicles
WO2005054495A2 (en) * 2003-11-26 2005-06-16 Applera Corporation Ligand-containing micelles and uses thereof
US20050239217A1 (en) * 2003-11-26 2005-10-27 Applera Corporation Fluorogenic homogeneous binding assay methods and compositions
JP2007514925A (ja) * 2003-12-01 2007-06-07 みらかホールディングス株式会社 光学バイオセンサー
US7304146B2 (en) * 2004-01-16 2007-12-04 Applera Corporation Fluorogenic kinase assays and substrates
US20060035302A1 (en) * 2004-06-21 2006-02-16 Applera Corporation Kinase substrates with multiple phosphorylation sites
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
US20060121553A1 (en) * 2004-10-29 2006-06-08 Applera Corporation Fluorogenic enzyme assay methods, kits and compositions using charge-balancers
US20060177918A1 (en) * 2004-12-30 2006-08-10 Applera Corporation Compositions, methods and kits for real-time enzyme assays using charged molecules
US20060240571A1 (en) * 2005-04-20 2006-10-26 Zahner Joseph E Biosensors and methods for detecting agents based upon time resolved luminescent resonance energy transfer
US7923240B2 (en) * 2006-03-31 2011-04-12 Intel Corporation Photo-activated field effect transistor for bioanalyte detection
US20090270269A1 (en) * 2008-04-28 2009-10-29 Ashok Kumar Nano-scale fluoro-biosensors exhibiting a low false alarm rate for rapid detection of biological contaminants
CN102725637B (zh) 2010-01-25 2015-02-25 松下健康医疗控股株式会社 在自组装单层上固定a蛋白的方法
JP5149992B2 (ja) 2010-08-30 2013-02-20 パナソニック株式会社 ストレプトアビジンを自己組織化膜上に固定する方法
WO2012053138A1 (ja) 2010-10-19 2012-04-26 パナソニック株式会社 グルコースオキシダーゼを自己組織化膜上に固定する方法
JP5202761B2 (ja) * 2011-06-10 2013-06-05 パナソニック株式会社 抗体を自己組織化膜上に固定する方法
EP4356132B1 (en) * 2021-06-17 2025-10-01 F. Hoffmann-La Roche AG Method for immunosensing on a lipid layer

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4254096A (en) * 1979-10-04 1981-03-03 Bio-Rad Laboratories, Inc. Reagent combination for solid phase immunofluorescent assay
US4598051A (en) * 1980-03-12 1986-07-01 The Regents Of The University Of California Liposome conjugates and diagnostic methods therewith
US4605630A (en) * 1983-07-27 1986-08-12 Cooper Lipotech Inc. Large-liposome agglutination reagent and method
US5011771A (en) * 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4971916A (en) * 1987-07-29 1990-11-20 Abbott Laboratories Liposome based homogeneous immunoassay for diagnostic tests
GB8807488D0 (en) 1988-03-29 1988-05-05 Ares Serono Res & Dev Ltd Method of assay
US5094819A (en) * 1989-06-16 1992-03-10 Washington Research Foundation Fluorescence-based optical sensor and method for detection of lipid-soluble analytes
ATE106566T1 (de) * 1989-11-21 1994-06-15 Bayer Ag Optischer biosensor.
WO1993010226A1 (en) * 1991-11-19 1993-05-27 North Carolina State University Immunodiagnostic assay using liposomes carrying labels thereof on outer liposome surface
DE4319037A1 (de) * 1993-06-08 1994-12-15 Bayer Ag Beschichtete Träger, Verfahren zu ihrer Herstellung und ihre Verwendung zur Immobilisierung von Biomolekülen an Oberlfächen von Festkörpern
WO1995008637A1 (en) 1993-09-21 1995-03-30 Washington State University Research Foundation Immunoassay comprising ligand-conjugated, ion channel receptor immobilized in lipid film
US5650334A (en) * 1995-08-31 1997-07-22 First Medical, Inc. Fluorescent labelling compositions and methods for their use
WO1997022719A1 (en) * 1995-12-18 1997-06-26 Washington University Method for nucleic acid analysis using fluorescence resonance energy transfer
US5780319A (en) * 1996-04-19 1998-07-14 Pasteur Sanofi Diagnostics Immunoassays to detect antiphospholipid antibodies

Also Published As

Publication number Publication date
JP2000515965A (ja) 2000-11-28
EP0932830B1 (en) 2002-10-16
WO1998000714A1 (en) 1998-01-08
DK0932830T3 (da) 2003-02-17
CA2257941A1 (en) 1998-01-08
FI102922B (fi) 1999-03-15
FI962686L (fi) 1997-12-29
NZ333472A (en) 2000-01-28
ATE226323T1 (de) 2002-11-15
NO986043D0 (no) 1998-12-22
NO986043L (no) 1998-12-22
PT932830E (pt) 2003-02-28
AU3264797A (en) 1998-01-21
DE69716454T2 (de) 2003-08-14
DE69716454D1 (de) 2002-11-21
FI962686A0 (fi) 1996-06-28
FI102922B1 (fi) 1999-03-15
AU722353B2 (en) 2000-07-27
JP3436374B2 (ja) 2003-08-11
US6235535B1 (en) 2001-05-22
EP0932830A1 (en) 1999-08-04

Similar Documents

Publication Publication Date Title
ES2181007T3 (es) Ensayo de interaccion de ligandos con transferencia de energia fluorescente sobre una pelicula lipidica.
ES2058164T3 (es) Conjugados fluorescentes y ensayo de diagnostico biologico.
AR031803A1 (es) Analisis electroquimico de coagulacion y dispositivo
ES2144405T3 (es) Soporte de ensayo para la ivnestigacion analitica de un liquido de muestra con ayuda de una reaccion de fijacion especifica de dos participes en la fijacion, bioafines.
ES2157669T3 (es) Un dispositivo para realizar un ensayo, uso de una membrana en la fabricacion de dicho dispositivo, kit que comprende dicho dispositivo, y metodo para la deteccion de un analito usando dicho dispositivo.
ES2173310T3 (es) Colorante con separadores universales/de transferencia de energia.
BRPI0608103A2 (pt) dispositivo para detecção e identificação rápida de microorganismos isolados sem cultura preliminar
AR053251A1 (es) Estrategias nuevas de etiquetado para la deteccion sensible de analitos
DE69617531D1 (de) Fluoreszierende Markierungskomplexe mit grossem Stokes-Shift die durch Verbindung von Cyaninen und anderen resonanzenergieübertragungfähigen Fluorochromen zusammengestellt sind
ES2035053T3 (es) Un aparato para monitorizar la concentracion de glucosa en un fluido corporal.
AU2001239178A1 (en) Device and method for detecting organic molecules in a test substance
ATE215698T1 (de) Maskierung der hintergrundfluoreszenz und - lumineszenz bei der optischen analyse biologisch medizinischer assays
EP2078952A3 (en) Rapid particle detection assay
AR076201A1 (es) Dispositivo y procedimiento para la deteccion y el analisis cuantitativo de analitos, particularmente de micotoxinas
WO2005114185A3 (en) Integrated glucose monitors and measurement of analytes via molecular oxygen modulation of dye fluorescence lifetime
ATE433572T1 (de) Methode, apparat, reagenzkit und reagenz zur unterscheidung von erythrozyten in biologischen proben
ATE272721T1 (de) Nukleinsäure hybridisierung-testverfahren in lösung
DE60224684D1 (de) Energiemessung von photonen von biologischen assays
Gaur et al. Emergence through delicate balance between the steric factor and molecular orientation: a highly bright and photostable DNA marker for real-time monitoring of cell growth dynamics
Zucker et al. Migrasome formation is initiated preferentially in tubular junctions by alternations of membrane tension or intracellular pressure
ES2187674T3 (es) Procedimiento de deteccion del cancer.
RU99110898A (ru) Экспресс-метод оценки степени тяжести состояния больных
Wicaksono et al. Cotton fiber-based assay with time-based microfluidic absorption sampling for point-of-care applications
Päkkilä et al. Simple and inexpensive immunoassay-based diagnostic tests
CN218445090U (zh) 药物检测装置