ES2173093T3 - Sistema de deteccion por fluorometro. - Google Patents

Sistema de deteccion por fluorometro.

Info

Publication number
ES2173093T3
ES2173093T3 ES93908431T ES93908431T ES2173093T3 ES 2173093 T3 ES2173093 T3 ES 2173093T3 ES 93908431 T ES93908431 T ES 93908431T ES 93908431 T ES93908431 T ES 93908431T ES 2173093 T3 ES2173093 T3 ES 2173093T3
Authority
ES
Spain
Prior art keywords
time
detection
histogram
compile
photon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93908431T
Other languages
English (en)
Inventor
Robert Marlin Studholme
David Arthur Blau
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hyperion Inc
Original Assignee
Hyperion Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hyperion Inc filed Critical Hyperion Inc
Application granted granted Critical
Publication of ES2173093T3 publication Critical patent/ES2173093T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging

Landscapes

  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Geophysics And Detection Of Objects (AREA)

Abstract

SE SUMINISTRA UN SISTEMA MEJORADO PARA LA DETECCION DE FLUORESCENCIA QUE UTILIZA UNA FUENTE DE LUZ DE ALTA VELOCIDAD DE REPETICION DE ALTA ENERGIA (10) CON DISPOSITIVOS ELECTRONICOS DE DETECCION DE ALTA VELOCIDAD PARA INCREMENTAR LA SENSIBILIDAD Y EXACTITUD DEL SISTEMA. EN UNA CONFORMACION PREFERIDA, LA FUENTE DE LUZ (10) ES UN DIODO LASER (90). EN UNA CONFORMACION, LA POSICION DE UNA VENTANA DE TIEMPO SE VARIA PARA COMPILAR UN PERFIL DE DESINTEGRACION DE UN FLUOROFORO. EN OTRA CONFORMACION, EL TIEMPO DE DETECCION DE UN FOTON SE UTILIZA PARA COMPILAR EL PERFIL DE DESINTEGRACION. EN UN ASPECTO DE ESTA INVENCION, SE GENERA UN HISTOGRAMA DE LA DESINTEGRACION DE FLUORESCENCIA DETERMINANDO UN HISTOGRAMA PRELIMINARMENTE DE LA FORMA Y MULTIPLICANDOLO POR LA PROPORCION DEL NUMERO TOTAL DE EVENTOS DIVIDIDOS POR EL NUMERO DE EVENTOS QUE COMPRENDEN EL HISTOGRAMA PRELIMINAR. EN OTRO ASPECTO DE LA INVENCION, EL TIEMPO DE DETECCION DESPUES DE LA EXCITACION DEL FOTON SE INICIA A PARTIR DE UN MOMENTO ALEATORIO, TAL COMO DESPUES DE QUE EL EVENTO PRECEDENTE SE HAYA DETECTADO Y LOS DATOS SE HAYAN ALMACENADOS.
ES93908431T 1992-03-23 1993-03-22 Sistema de deteccion por fluorometro. Expired - Lifetime ES2173093T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/855,238 US5323008A (en) 1992-03-23 1992-03-23 Fluorometer detection system

Publications (1)

Publication Number Publication Date
ES2173093T3 true ES2173093T3 (es) 2002-10-16

Family

ID=25320715

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93908431T Expired - Lifetime ES2173093T3 (es) 1992-03-23 1993-03-22 Sistema de deteccion por fluorometro.

Country Status (8)

Country Link
US (1) US5323008A (es)
EP (1) EP0632887B1 (es)
JP (2) JPH07505467A (es)
AT (1) ATE213832T1 (es)
CA (2) CA2538701C (es)
DE (1) DE69331629T2 (es)
ES (1) ES2173093T3 (es)
WO (1) WO1993019358A1 (es)

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US6121053A (en) * 1997-12-10 2000-09-19 Brookhaven Science Associates Multiple protocol fluorometer and method
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AU5223899A (en) 1998-07-27 2000-02-21 Ljl Biosystems, Inc. Apparatus and methods for spectroscopic measurements
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JP2001070227A (ja) * 1999-07-07 2001-03-21 Fuji Photo Film Co Ltd 蛍光観察装置
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US6447995B1 (en) 2000-10-04 2002-09-10 Genvec, Inc. Utilizing intrinsic fluorescence to detect adenovirus
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US20030136921A1 (en) * 2002-01-23 2003-07-24 Reel Richard T Methods for fluorescence detection that minimizes undesirable background fluorescence
JP4074136B2 (ja) * 2002-05-29 2008-04-09 浜松ホトニクス株式会社 蛍光寿命分布画像測定装置およびその測定方法
US20030224354A1 (en) * 2002-05-30 2003-12-04 Introgen Therapeutics Inc. Quantifying viral particles with intrinsic fluorescence
JP4188653B2 (ja) * 2002-10-01 2008-11-26 浜松ホトニクス株式会社 蛍光測定装置
US7371524B2 (en) * 2003-06-17 2008-05-13 Hannjorg Hereth Substituted azaporphyrins as fluorescence labels
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JP4652868B2 (ja) * 2005-03-30 2011-03-16 独立行政法人産業技術総合研究所 蛍光分析方法
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Also Published As

Publication number Publication date
DE69331629T2 (de) 2002-08-14
CA2538701C (en) 2006-11-28
ATE213832T1 (de) 2002-03-15
CA2132707A1 (en) 1993-09-30
CA2132707C (en) 2006-06-13
US5323008A (en) 1994-06-21
EP0632887B1 (en) 2002-02-27
JP2002323448A (ja) 2002-11-08
JPH07505467A (ja) 1995-06-15
EP0632887A1 (en) 1995-01-11
EP0632887A4 (en) 1995-11-22
CA2538701A1 (en) 1993-09-30
WO1993019358A1 (en) 1993-09-30
DE69331629D1 (de) 2002-04-04

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