ES2148202T3 - Fijador celular y procedimiento de coloracion de celulas sin destruccion de la superficie celular. - Google Patents

Fijador celular y procedimiento de coloracion de celulas sin destruccion de la superficie celular.

Info

Publication number
ES2148202T3
ES2148202T3 ES93302372T ES93302372T ES2148202T3 ES 2148202 T3 ES2148202 T3 ES 2148202T3 ES 93302372 T ES93302372 T ES 93302372T ES 93302372 T ES93302372 T ES 93302372T ES 2148202 T3 ES2148202 T3 ES 2148202T3
Authority
ES
Spain
Prior art keywords
cell
cellular
components
fixative composition
compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93302372T
Other languages
English (en)
Inventor
Mark Carle Connelly
Houston G Brooks Jr
Utpal R Chakraborty
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Ortho Diagnostic Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Diagnostic Systems Inc filed Critical Ortho Diagnostic Systems Inc
Application granted granted Critical
Publication of ES2148202T3 publication Critical patent/ES2148202T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/108331—Preservative, buffer, anticoagulant or diluent
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • AIDS & HIV (AREA)
  • Zoology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

LA PRESENTE INVENCION SUMINISTRA UNA NUEVA COMPOSICION FIJADORA CELULAR Y METODO DE FIJACION CELULAR Y ANALISIS CELULAR DONDE LAS CELULAS PUEDEN SER FIJADAS PARA POSTERIORES ANALISIS SIN DESTRUIR LAS MARCAS DE LA SUPERFICIE DE LA CELULA, LA MORFOLOGIA DE LA CELULA Y LAS PROPIEDADES DE DISPERSION DE LA LUZ DE LA CELULA. SE DESCRIBE TAMBIEN EL TRATAMIENTO DE CELULAS CON EL FIJADOR DE DONDE SE PERMITE TAMBIEN ANTICUERPOS U OTROS COMPONENTES DESEADOS PARA ENTRAR EN LA CELULA A TRAVES DE LA MEMBRANA CELULAR, SIN PERMITIR QUE SE ESCAPEN CONTENIDOS IMPORTANTES DE LA CELULA. COMO UN BENEFICIO AÑADIDO, LA COMPOSICION FIJADORA DE LA INVENCION PUEDE TAMBIEN MATAR O AL MENOS INACTIVAR O REDUCIR LA ACTIVIDAD DE VIRUS MORTALES CONTENIDOS EN LA CELULA. EN UN ASPECTO MAS AMPLIO, LA PRESENTE INVENCION RECLAMA COMPOSICIONES FIJADORAS QUE COMPRENDEN AL MENOS TRES COMPONENTES, Y EN UNAS INCORPORACIONES MAS PREFERENTES, CUATRO COMPONENTES. EN UNA INCORPORACION PARTICULARMENTE PREFERENTE, LA COMPOSICION FIJADORADESCRITA COMPRENDE AL MENOS UN COMPUESTO ADECUADO PARA EL INCREMENTO DE LA PERMEABILIDAD DE UNA MEMBRANA CELULAR, AL MENOS UN COMPUESTO QUE FACILITA EL TRANSPORTE DE LOS COMPONENTES A TRAVES DE LAS MEMBRANAS CELULARES, AL MENOS UN DETERGENTE, Y AL MENOS UN COMPUESTO TENIENDO LA ESTRUCTURA: R1,R2,R3,R4,R5
ES93302372T 1992-03-27 1993-03-26 Fijador celular y procedimiento de coloracion de celulas sin destruccion de la superficie celular. Expired - Lifetime ES2148202T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/859,212 US5422277A (en) 1992-03-27 1992-03-27 Cell fixative composition and method of staining cells without destroying the cell surface

Publications (1)

Publication Number Publication Date
ES2148202T3 true ES2148202T3 (es) 2000-10-16

Family

ID=25330350

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93302372T Expired - Lifetime ES2148202T3 (es) 1992-03-27 1993-03-26 Fijador celular y procedimiento de coloracion de celulas sin destruccion de la superficie celular.

Country Status (9)

Country Link
US (2) US5422277A (es)
EP (1) EP0562877B1 (es)
JP (1) JP3651912B2 (es)
AT (1) ATE193944T1 (es)
CA (1) CA2092866C (es)
DE (1) DE69328846T2 (es)
DK (1) DK0562877T3 (es)
ES (1) ES2148202T3 (es)
GR (1) GR1002073B (es)

Families Citing this family (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422277A (en) * 1992-03-27 1995-06-06 Ortho Diagnostic Systems Inc. Cell fixative composition and method of staining cells without destroying the cell surface
AU2593192A (en) * 1992-09-14 1994-04-12 Oystein Fodstad Detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations
GB9223035D0 (en) * 1992-11-03 1992-12-16 Kiessling Cooper Ann A Preservation of peripheral blood & semen in fixatives that inactivate human pathogens
DE69327775T2 (de) * 1992-11-19 2000-06-21 Sysmex Corp Verfahren zur Vorbehandlung für Blutanalyse
DZ1781A1 (fr) * 1993-05-21 2002-02-17 Radopah Ltd Agents d'arylation.
GB2279653B (en) * 1993-07-05 1998-02-11 North Gen Hospital Nhs Trust Stabilisation of cells with an agent comprising a heavy metal compound
DE4343461C1 (de) * 1993-12-20 1995-06-14 Erno Raumfahrttechnik Gmbh Verfahren zur Fixierung biologischer Präparate
NO180658C (no) 1994-03-10 1997-05-21 Oeystein Fodstad Fremgangsmåte og anordning for deteksjon av spesifikke målceller i spesialiserte eller blandede cellepopulasjoner og opplösninger som inneholder blandede cellepopulasjoner
EP0754301B1 (en) * 1994-04-05 1998-09-09 Northern General Hospital N.H.S. Trust Preparation and stabilisation of cell suspensions
US5648222A (en) * 1994-07-27 1997-07-15 The Trustees Of Columbia University In The City Of New York Method for preserving cells, and uses of said method
US5648220A (en) * 1995-02-14 1997-07-15 New England Medical Center Hospitals, Inc. Methods for labeling intracytoplasmic molecules
NO961031D0 (no) 1996-03-13 1996-03-13 Det Norske Radiumshospital Tum Fremgangsmåte til å drepe uönskede målceller
EP0834742A1 (de) * 1996-10-01 1998-04-08 Gull Laboratories GmbH Spezifischer Anti-EBNA-1-Test mittels antikomplementärer Immunfluoreszenz
US7521176B2 (en) * 1996-10-15 2009-04-21 Theranostech, Inc. Methods for characterizing the viral infectivity status of a host
US5811303A (en) * 1997-04-01 1998-09-22 Streck Laboratories, Inc. Quantitative buffy coat control composition
GB9716454D0 (en) * 1997-08-05 1997-10-08 Univ St Andrews Polymer
US6793890B2 (en) 1997-08-20 2004-09-21 The University Of Miami Rapid tissue processor
EP1005633B1 (en) 1997-08-20 2008-09-03 The University Of Miami A high quality, continuous throughput, tissue fixation-dehydration-fat removal-impregnation method
US6664045B1 (en) * 1998-06-18 2003-12-16 Boston Probes, Inc. PNA probes, probe sets, methods and kits pertaining to the detection of microorganisms
CA2335747A1 (en) * 1998-06-30 2000-01-06 Lamina, Inc. Cytological and histological fixative composition and methods of use
US6280946B2 (en) * 1998-08-07 2001-08-28 Boston Probes, Inc. PNA probes, probe sets, methods and kits pertaining to the universal detection of bacteria and eucarya
US6143512A (en) * 1998-08-17 2000-11-07 Markovic; Nenad Cap-pap test
AU769539B2 (en) * 1999-01-29 2004-01-29 Zoetis Services Llc Adjuvants for use in vaccines
US20010016317A1 (en) * 1999-09-13 2001-08-23 Dolores M. Berger Method for providing long term stability to cells for diagnostic testing
US6916608B2 (en) * 1999-09-10 2005-07-12 Becton, Dickinson And Company Composition for providing long term stability to cells for diagnostic testing
NL1015352C2 (nl) * 2000-05-31 2001-12-03 Charles Edward Strother Iii Antigen conjugatie vaccinatie techniek met gastheer virus.
JP2004502434A (ja) * 2000-07-04 2004-01-29 バイオイメージ　エイ／エス 細胞成分間の相互作用に関する量的情報の抽出方法
US6828109B2 (en) * 2000-12-15 2004-12-07 James R. Bell, Jr. Methods for detecting an analyte of interest using catalyzed reporter deposition of tyramide
GB0111442D0 (en) * 2001-05-10 2001-07-04 Isis Innovation Method for preparing recombinant virus
US20030228635A1 (en) * 2002-02-15 2003-12-11 Renovar, Inc. Cell proliferation assays and methods
US7138226B2 (en) 2002-05-10 2006-11-21 The University Of Miami Preservation of RNA and morphology in cells and tissues
CA2428740A1 (en) * 2002-05-20 2003-11-20 Bayer Corporation Automated method and reagent therefor for assaying body fluid samples such as cerebrospinal fluid (csf)
DE60308259T2 (de) * 2002-05-22 2007-04-05 Sysmex Corp. Immunologische Verfahren, Vorrichtungen und Reagenzien
DE10305768A1 (de) * 2003-02-11 2004-08-19 Justus-Liebig-Universität Giessen Verfahren zum Färben von lipophilen Zellbestandteilen einer biologischen Probe
US7041481B2 (en) * 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US7267980B1 (en) 2003-04-04 2007-09-11 Research & Diagnostic Systems, Inc. Stabilizing solution for cells and tissues
US20040197836A1 (en) * 2003-04-04 2004-10-07 Hashemi Brian B. Measurement of F-actin in whole blood cellular subsets
US20050069958A1 (en) * 2003-09-26 2005-03-31 Mills Rhonda A. Method for simultaneous evaluation of a sample containing a cellular target and a soluble analyte
US7326577B2 (en) * 2003-10-20 2008-02-05 Esoterix, Inc. Cell fixation and use in phospho-proteome screening
ES2411962T3 (es) 2003-10-24 2013-07-09 Gencia Corporation Métodos y composiciones para suministrar polinucleótidos
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
US8507277B2 (en) 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
US8062891B2 (en) 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
US7470401B2 (en) 2003-10-24 2008-12-30 The University Of Miami Simplified tissue processing
US20090208478A1 (en) * 2003-10-24 2009-08-20 Gencia Corporation Transducible polypeptides for modifying metabolism
US20050266503A1 (en) * 2004-05-24 2005-12-01 Esoterix, Inc. Simultaneous assay of target and target-drug binding
US7803523B2 (en) * 2004-08-27 2010-09-28 University Health Network Whole blood preparation for cytometric analysis of cell signaling pathways
WO2006090096A1 (en) * 2005-02-28 2006-08-31 Sheffield Teaching Hospitals Nhs Foundation Trust Composition, kit and method for fixing cells
US8758990B2 (en) * 2005-07-28 2014-06-24 Id-Fish Technology, Inc. Methods for detecting and differentiating Mycobacterium genus and Mycobacterium avium complex in a sample or culture
ZA200801859B (en) * 2005-07-28 2009-07-29 Id Fish Technology Inc Method for improving cell permeability to foreign particles
DE102006040315B4 (de) * 2006-08-29 2008-06-05 Biosepar -Gesellschaft für Medizin- und Labortechnik mbH Fixativum zum Fixieren von biologischen Materialien
US20090269800A1 (en) * 2008-04-29 2009-10-29 Todd Covey Device and method for processing cell samples
US20090298172A1 (en) 2008-05-28 2009-12-03 Steven Paul Wheeler Histological specimen treatment apparatus and method
US20100014741A1 (en) * 2008-07-10 2010-01-21 Banville Steven C Methods and apparatus related to gate boundaries within a data space
US9183237B2 (en) 2008-07-10 2015-11-10 Nodality, Inc. Methods and apparatus related to gate boundaries within a data space
GB2474613A (en) * 2008-07-10 2011-04-20 Nodality Inc Methods and apparatus related to management of experiments
US20100030719A1 (en) * 2008-07-10 2010-02-04 Covey Todd M Methods and apparatus related to bioinformatics data analysis
US7968279B2 (en) * 2008-08-13 2011-06-28 Beckman Coulter, Inc. Reference control for cell by cell analysis
JP5674664B2 (ja) 2008-09-04 2015-02-25 ベックマン コールター， インコーポレイテッド パンキナーゼ活性化およびシグナリング経路の評価
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9598725B2 (en) * 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9034257B2 (en) * 2008-10-27 2015-05-19 Nodality, Inc. High throughput flow cytometry system and method
US20100233733A1 (en) * 2009-02-10 2010-09-16 Nodality, Inc., A Delaware Corporation Multiple mechanisms for modulation of the pi3 kinase pathway
EP2940153B1 (en) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
AU2011230619C1 (en) 2010-03-25 2016-06-23 Oregon Health & Science University CMV glycoproteins and recombinant vectors
JP5922139B2 (ja) 2010-11-01 2016-05-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド エマルジョンを形成するためのシステム
ES2627069T3 (es) * 2010-11-12 2017-07-26 Incelldx, Inc. Métodos y sistemas para predecir si un sujeto tiene una lesión de neoplasia intraepitelial cervical (CIN) a partir de una muestra de células cervicales
CN103534360A (zh) 2011-03-18 2014-01-22 伯乐生命医学产品有限公司 借助对信号的组合使用进行的多重数字分析
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
MX361688B (es) 2011-05-02 2018-12-13 Jose Carlos Lapenna Uso de un halógeno-cianoacetamida para fijación y preservación de una muestra biológica.
LT2691530T (lt) 2011-06-10 2018-08-10 Oregon Health & Science University Cmv glikoproteinai ir rekombinantiniai vektoriai
EP2737089B1 (en) 2011-07-29 2017-09-06 Bio-rad Laboratories, Inc. Library characterization by digital assay
US20130189754A1 (en) 2011-09-12 2013-07-25 International Aids Vaccine Initiative Immunoselection of recombinant vesicular stomatitis virus expressing hiv-1 proteins by broadly neutralizing antibodies
US9402894B2 (en) 2011-10-27 2016-08-02 International Aids Vaccine Initiative Viral particles derived from an enveloped virus
EP3104177B1 (en) * 2011-12-21 2020-11-25 Beckman Coulter, Inc. Method for labeling intracellular and extracellular targets of leukocytes
EP2679596B1 (en) 2012-06-27 2017-04-12 International Aids Vaccine Initiative HIV-1 env glycoprotein variant
JP6311707B2 (ja) * 2013-05-13 2018-04-18 コニカミノルタ株式会社 細胞染色方法及びその方法に使用する検体採取管
US20150065381A1 (en) 2013-09-05 2015-03-05 International Aids Vaccine Initiative Methods of identifying novel hiv-1 immunogens
US10058604B2 (en) 2013-10-07 2018-08-28 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
US9733162B2 (en) 2014-02-11 2017-08-15 Ihor Turkevych Universal system, method and solution for the acceleration of the process of fixing, dehydrating and clearing the structure of biological tissue
US10174292B2 (en) 2015-03-20 2019-01-08 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
EP3072901A1 (en) 2015-03-23 2016-09-28 International Aids Vaccine Initiative Soluble hiv-1 envelope glycoprotein trimers
US10006084B2 (en) 2015-04-30 2018-06-26 Sakura Finetek U.S.A., Inc. Methods to reduce evaporation during elevated temperature
US10365189B2 (en) 2015-05-07 2019-07-30 Steven Wheeler Histological specimen treatment
US9835527B2 (en) 2015-12-30 2017-12-05 Sakura Finetek U.S.A., Inc. Tissue processing reagent
US10107727B2 (en) 2015-12-30 2018-10-23 Sakura Finetek U.S.A., Inc. Tissue processing reagent
WO2017152028A1 (en) * 2016-03-04 2017-09-08 Janssen Diagnostics, Llc Preservation of cellular components from cells with a preservative

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU552538A1 (ru) * 1973-07-31 1977-03-30 Кубанский Государственный Медицинский Институт Им. Красной Армии Способ вы влени свободного цитоплазматического катионного белка лейкоцитов в микроскопических препаратах
US4423153A (en) * 1981-12-03 1983-12-27 Board Of Regents, The University Of Texas System Methods and compositions for the detection and determination of cellular DNA
US4499183A (en) * 1982-12-21 1985-02-12 Ortho Diagnostic Systems Inc. Detecting intracellular antigens in swelled and fixed cells with labeled antibody
JPS6161055A (ja) * 1984-08-31 1986-03-28 Shionogi & Co Ltd ウイルス赤血球凝集反応試験用鳥類固定赤血球の保存液
US4575452A (en) * 1984-09-21 1986-03-11 E. I. Du Pont De Nemours And Company Kit for silver staining proteins and nucleic acids
GB2192712B (en) * 1986-06-10 1990-02-14 Lab Supplies And Instr Fixative
US4698312A (en) * 1986-07-28 1987-10-06 Fisher Scientific Company Stabilizing blood cells with aromatic polyaldehyde for use in hematology controls and calibrators
JPH06100596B2 (ja) * 1986-09-10 1994-12-12 東亜医用電子株式会社 フロ−サイトメトリ−による白血球の分類方法
DE3824936A1 (de) * 1988-06-28 1990-03-22 Schubert Werner Schnellfixierung
US5422277A (en) * 1992-03-27 1995-06-06 Ortho Diagnostic Systems Inc. Cell fixative composition and method of staining cells without destroying the cell surface

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DE69328846T2 (de) 2000-11-09
JP3651912B2 (ja) 2005-05-25
ATE193944T1 (de) 2000-06-15
GR1002073B (en) 1995-12-04
US5597688A (en) 1997-01-28
EP0562877B1 (en) 2000-06-14
EP0562877A2 (en) 1993-09-29
EP0562877A3 (en) 1993-12-29
DE69328846D1 (de) 2000-07-20
GR930100119A (el) 1993-11-30
US5422277A (en) 1995-06-06
CA2092866C (en) 2007-06-26
JPH0678792A (ja) 1994-03-22
CA2092866A1 (en) 1993-09-28
DK0562877T3 (da) 2000-07-31

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