ES2081861T3 - Inmunoensayo de polarizacion de fluorescencia para la metadona. - Google Patents

Inmunoensayo de polarizacion de fluorescencia para la metadona.

Info

Publication number
ES2081861T3
ES2081861T3 ES90101208T ES90101208T ES2081861T3 ES 2081861 T3 ES2081861 T3 ES 2081861T3 ES 90101208 T ES90101208 T ES 90101208T ES 90101208 T ES90101208 T ES 90101208T ES 2081861 T3 ES2081861 T3 ES 2081861T3
Authority
ES
Spain
Prior art keywords
metadone
fluorescence polarization
methadone
sample
immuno assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90101208T
Other languages
English (en)
Inventor
Robert E Dubler
John A Walling
Susan A Thacker
Nai-Yi Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2081861T3 publication Critical patent/ES2081861T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9486—Analgesics, e.g. opiates, aspirine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Emergency Medicine (AREA)
  • Pain & Pain Management (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

SE PROPORCIONA UN INMUNO-ENSAYO DE POLARIZACION POR FLUORESCENCIA PARA DETECTAR LA PRESENCIA DE METADONA EN UNA MUESTRA DE ENSAYO, EN BASE A LA COMPETENCIA ENTRE LA METADONA Y UN TRAZADOR ETIQUETADO FLUORESCENTEMENTE PARA LOS LUGARES DE ENLACE DE UN ANTICUERPO ESPECIFICO PARA LA METADONA. LA CONCENTRACION DE METADONA EN LA MUESTRA DETERMINA LA CANTIDAD DE TRAZADOR QUE SE UNE CON EL ANTICUERPO. LA CANTIDAD DEL COMPLEJO TRAZADOR-ANTICUERPO FORMADA PUEDE MEDIRSE CUANTITATIVAMENTE Y ES INVERSAMENTE PROPORCIONAL A LA CANTIDAD DE METADONA EN LA MUESTRA. SE DESCRIBEN TAMBIEN LOS TRAZADORES UTILIZADOS PARA PRODUCIR ANTICUERPOS E INMUNOGENOS UTILIZADOS PARA PRODUCIR ANTICUERPOS PARA UTILIZACION COMO REACTIVOS.
ES90101208T 1989-01-23 1990-01-22 Inmunoensayo de polarizacion de fluorescencia para la metadona. Expired - Lifetime ES2081861T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/300,670 US5073629A (en) 1989-01-23 1989-01-23 Methadone fluorescence polarization immunoassay

Publications (1)

Publication Number Publication Date
ES2081861T3 true ES2081861T3 (es) 1996-03-16

Family

ID=23160102

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90101208T Expired - Lifetime ES2081861T3 (es) 1989-01-23 1990-01-22 Inmunoensayo de polarizacion de fluorescencia para la metadona.

Country Status (6)

Country Link
US (1) US5073629A (es)
EP (3) EP0875762A2 (es)
JP (1) JPH02247157A (es)
CA (1) CA2008277A1 (es)
DE (1) DE69023357T2 (es)
ES (1) ES2081861T3 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212096A (en) * 1992-02-19 1993-05-18 University Of Colorado Foundation, Inc. Gas chromatography/mass spectrometry determination of ascorbate and oxidation products thereof
US5710256A (en) * 1995-04-03 1998-01-20 Biosite Diagnostics Incorporated Methadone derivatives and protein and polypeptide methadone derivative conjugates and labels
WO1996040662A2 (en) * 1995-06-07 1996-12-19 Cellpro, Incorporated Aminooxy-containing linker compounds and their application in conjugates
EP0931062B1 (en) * 1997-05-27 2004-11-17 Microgenics Corporation Conjugates and specific immunoassays for the methadone metabolite 2-ethylidine-1,5-dimethyl-3,3-diphenylpyrrolidine
US8771964B2 (en) 2012-02-02 2014-07-08 Siemens Healthcare Diagnostics Inc. Compositions and methods for detection of methadone metabolite
CN102617730B (zh) * 2012-04-11 2013-07-31 杭州培乐生物技术有限公司 一种美沙酮人工抗原的制备方法
US10101278B2 (en) * 2013-05-09 2018-10-16 University Of Central Florida Research Foundation, Inc. Portable spectrometer for the presumptive identification of illicit drugs and substances of abuse
US11199498B2 (en) 2013-05-09 2021-12-14 University Of Central Florida Research Foundation, Inc. Portable spectrometer for the presumptive identification of substances

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3940475A (en) * 1970-06-11 1976-02-24 Biological Developments, Inc. Radioimmune method of assaying quantitatively for a hapten
US4097586A (en) * 1970-06-11 1978-06-27 Biological Developments, Inc. Immunochemical assay method
US3704282A (en) * 1971-04-09 1972-11-28 Sidney Spector Catecholamine antigens and antibodies specific therefor
US3817837A (en) * 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3996344A (en) * 1972-05-15 1976-12-07 Biological Developments, Inc. Phenethylamine antigenic conjugates, their preparation, antibodies and use
US4022878A (en) * 1972-05-15 1977-05-10 Biological Developments, Inc. Methods and compounds for producing specific antibodies
US3843696A (en) * 1972-09-05 1974-10-22 Syva Co Methadone analog compounds
US3966556A (en) * 1972-11-06 1976-06-29 Syva Company Compounds for enzyme amplification assay methadone analogs
US4255329A (en) * 1973-10-02 1981-03-10 Syva Company Double receptor fluorescent immunoassay
US4041076A (en) * 1974-10-23 1977-08-09 Hoffmann-La Roche Inc. Immunoassay for pharmacologically active phenethylamines
US4016146A (en) * 1974-12-10 1977-04-05 Biological Developments, Inc. Phenethylamine antigenic conjugates, their preparation, antibodies, and use
JPS51101120A (en) * 1975-02-28 1976-09-07 Dai Ichi Kogyo Seiyaku Co Ltd Kogen oyobi kotainoseiho
US4104367A (en) * 1976-10-13 1978-08-01 Hoffmann-La Roche Inc. Radioimmunoassay for methadone
US4329281A (en) * 1978-06-05 1982-05-11 Hoffmann-La Roche Inc. Hapten compositions
US4588697A (en) * 1979-09-07 1986-05-13 Syntex (U.S.A.) Inc. Method for performing fluorescent protein binding assay employing novel alkyl substituted fluorescent compounds and conjugates
US4351760A (en) * 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4481136A (en) * 1979-09-07 1984-11-06 Syva Company Alkyl substituted fluorescent compounds and conjugates
JPS56125666A (en) * 1980-03-07 1981-10-02 Fujisawa Pharmaceut Co Ltd Immuno-analytical method for amphetamines and reagent thereof
US4593089A (en) * 1980-07-30 1986-06-03 Abbott Laboratories Fluorescent polarization immunoassay utilizing substituted triazinylaminofluorescein aminoglycosides
US4420568A (en) * 1980-07-30 1983-12-13 Abbott Laboratories Fluorescent polarization immunoassay utilizing substituted triazinylaminofluoresceins
AU555213B2 (en) * 1981-02-17 1986-09-18 Abbott Laboratories N-substituted-amido-fluoresein derivatives
US4492762A (en) * 1981-12-11 1985-01-08 Abbott Laboratories Fluorescent polarization immunoassays
US4585862A (en) * 1981-12-11 1986-04-29 Abbott Laboratories Fluorescence polarization immunoassay
US4510251A (en) * 1982-11-08 1985-04-09 Abbott Laboratories Fluorescent polarization assay for ligands using aminomethylfluorescein derivatives as tracers
US4476229A (en) * 1982-11-08 1984-10-09 Abbott Laboratories Substituted carboxyfluoresceins
US4476228A (en) * 1982-11-08 1984-10-09 Abbott Laboratories Determination of unsaturated thyroxine binding protein sites using fluorescence polarization techniques
US4681859A (en) * 1984-09-21 1987-07-21 Ortho Diagnostic Systems Inc. Fluorescence polarization immunoassay for heavy antigens
EP0199042A1 (en) * 1985-03-26 1986-10-29 Abbott Laboratories Procainamide assay, tracers, immunogens and antibodies
EP0201751A2 (en) * 1985-05-08 1986-11-20 Abbott Laboratories 4'-aminomethyfluorescein derivatives for use in fluorescence polarization immunoassys
EP0218010A3 (en) * 1985-07-10 1988-01-07 Abbott Laboratories Ligand detection method and substituted carboxyfluorescein tracers therefor
US4751190A (en) * 1985-07-22 1988-06-14 Abbott Laboratories Fluorescence polarization immunoassay and reagents for use therein

Also Published As

Publication number Publication date
EP0380019A2 (en) 1990-08-01
US5073629A (en) 1991-12-17
DE69023357T2 (de) 1996-06-20
EP0380019A3 (en) 1992-02-12
EP0380019B1 (en) 1995-11-08
EP0662613A1 (en) 1995-07-12
JPH02247157A (ja) 1990-10-02
EP0875762A2 (en) 1998-11-04
CA2008277A1 (en) 1990-07-23
DE69023357D1 (de) 1995-12-14

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