GB2008247A - Detecting or quantifying substances using labelling techniques - Google Patents

Detecting or quantifying substances using labelling techniques

Info

Publication number
GB2008247A
GB2008247A GB7844457A GB7844457A GB2008247A GB 2008247 A GB2008247 A GB 2008247A GB 7844457 A GB7844457 A GB 7844457A GB 7844457 A GB7844457 A GB 7844457A GB 2008247 A GB2008247 A GB 2008247A
Authority
GB
United Kingdom
Prior art keywords
luminescent
detecting
substance
antigens
quantifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7844457A
Other versions
GB2008247B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Welsh National School of Medicine
Original Assignee
Welsh National School of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Welsh National School of Medicine filed Critical Welsh National School of Medicine
Priority to GB7844457A priority Critical patent/GB2008247B/en
Publication of GB2008247A publication Critical patent/GB2008247A/en
Application granted granted Critical
Publication of GB2008247B publication Critical patent/GB2008247B/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Plasma & Fusion (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

A method of quantifying a protein, antibody, antigen or hapten includes linking the substance to a chemi-luminescent or bio-luminescent label and triggering a luminescent reaction by the addition of an oxidising agent or a catalyst. The emitted light is observed and this provides information about the substance of interest. In one aspect, the invention employs a luminescent reagent which consists of antibodies labelled with a luminescent material such as luminol to quantify antigens in an immunological assay, in which the luminescent labelled antibodies selectively bind to the antigens and the amount of light emitted is measured.
GB7844457A 1977-11-17 1978-11-14 Detecting or quantifying substances using labelling techniques Expired GB2008247B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB7844457A GB2008247B (en) 1977-11-17 1978-11-14 Detecting or quantifying substances using labelling techniques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB4783977 1977-11-17
GB7844457A GB2008247B (en) 1977-11-17 1978-11-14 Detecting or quantifying substances using labelling techniques

Publications (2)

Publication Number Publication Date
GB2008247A true GB2008247A (en) 1979-05-31
GB2008247B GB2008247B (en) 1982-12-15

Family

ID=26266148

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7844457A Expired GB2008247B (en) 1977-11-17 1978-11-14 Detecting or quantifying substances using labelling techniques

Country Status (1)

Country Link
GB (1) GB2008247B (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0044140A1 (en) * 1980-06-13 1982-01-20 National Research Development Corporation Binding assays
EP0070686A2 (en) * 1981-07-17 1983-01-26 Amoco Corporation Non-radiative energy transfer immunochemical technique
EP0070832A1 (en) * 1981-02-10 1983-02-09 Electro-Nucleonics, Inc. Microencapsulated fluorescer composition, test kit and system for immunoassay
EP0082636A1 (en) * 1981-12-11 1983-06-29 The Welsh National School of Medicine Luminescent labelling materials and procedures
EP0087959A1 (en) * 1982-03-03 1983-09-07 National Research Development Corporation Enhanced luminescent and luminometric assay
EP0103469A2 (en) * 1982-09-10 1984-03-21 The Welsh National School of Medicine Immunological procedure for quantifying substances
EP0116454A2 (en) * 1983-02-11 1984-08-22 National Research Development Corporation Enhanced luminescent or luminometric assay
DE3628573A1 (en) * 1986-08-22 1988-02-25 Hoechst Ag CHEMILUMINESCENT ACRIDINE DERIVATIVES AND THEIR USE IN LUMINESCENCE IMMUNOASSAYS
WO1991000511A1 (en) * 1989-06-24 1991-01-10 University Of Wales College Of Medicine Detecting or quantifying multiple analytes using labelling techniques
DE3844954C2 (en) * 1988-02-20 1998-07-16 Hoechst Ag Special chemiluminescent acridine derivatives and their use in luminescent immunoassays
US5783696A (en) * 1988-02-20 1998-07-21 Hoechst Aktiengesellschaft Special chemiluminescent acridine derivatives
US6087476A (en) * 1988-03-24 2000-07-11 Igen International, Inc. Luminescent chimeric proteins

Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0044140A1 (en) * 1980-06-13 1982-01-20 National Research Development Corporation Binding assays
EP0070832A1 (en) * 1981-02-10 1983-02-09 Electro-Nucleonics, Inc. Microencapsulated fluorescer composition, test kit and system for immunoassay
EP0070832A4 (en) * 1981-02-10 1983-06-17 Electro Nucleonics Microencapsulated fluorescer composition, test kit and system for immunoassay.
EP0070686A2 (en) * 1981-07-17 1983-01-26 Amoco Corporation Non-radiative energy transfer immunochemical technique
EP0070686A3 (en) * 1981-07-17 1983-04-13 Standard Oil Company Non-radiative energy transfer immunochemical technique
EP0082636A1 (en) * 1981-12-11 1983-06-29 The Welsh National School of Medicine Luminescent labelling materials and procedures
US4946958A (en) * 1981-12-11 1990-08-07 The Welsh National School Of Medicine Chemiluminescent acridinium labelling compounds
EP0087959A1 (en) * 1982-03-03 1983-09-07 National Research Development Corporation Enhanced luminescent and luminometric assay
WO1983003104A1 (en) * 1982-03-03 1983-09-15 Carter, Timothy, Joseph, Nicholas Enhanced luminescent and luminometric assay
US4842997A (en) * 1982-03-03 1989-06-27 National Research Development Corporation Enhanced luminescent and luminometric assay
JPH0453518B2 (en) * 1982-03-03 1992-08-26 Nat Res Dev
EP0103469A2 (en) * 1982-09-10 1984-03-21 The Welsh National School of Medicine Immunological procedure for quantifying substances
EP0103469A3 (en) * 1982-09-10 1984-12-05 The Welsh National School Of Medicine Immunological procedure for detecting or quantifying substances
GB2129553A (en) * 1982-09-10 1984-05-16 Welsh Nat School Med Immunological procedure for detecting or quantifying substances
EP0116454A3 (en) * 1983-02-11 1985-12-18 National Research Development Corporation Enhanced luminescent or luminometric assay
US4598044A (en) * 1983-02-11 1986-07-01 National Research Development Corporation Enhanced luminescent or luminometric assay
EP0116454A2 (en) * 1983-02-11 1984-08-22 National Research Development Corporation Enhanced luminescent or luminometric assay
DE3628573A1 (en) * 1986-08-22 1988-02-25 Hoechst Ag CHEMILUMINESCENT ACRIDINE DERIVATIVES AND THEIR USE IN LUMINESCENCE IMMUNOASSAYS
EP0257541A3 (en) * 1986-08-22 1988-11-30 Hoechst Aktiengesellschaft Chemilumuniscent acridine derivatives and their use in luminescence immunoassays
EP0257541A2 (en) * 1986-08-22 1988-03-02 Hoechst Aktiengesellschaft Chemilumuniscent acridine derivatives and their use in luminescence immunoassays
EP0647628A1 (en) * 1986-08-22 1995-04-12 Hoechst Aktiengesellschaft Acridmium derivatives and their use in luminescence immunoassays
DE3645292C2 (en) * 1986-08-22 1997-12-11 Hoechst Ag New chemiluminescent 9-carboxy-acridinium cpds.
DE3844954C2 (en) * 1988-02-20 1998-07-16 Hoechst Ag Special chemiluminescent acridine derivatives and their use in luminescent immunoassays
US5783696A (en) * 1988-02-20 1998-07-21 Hoechst Aktiengesellschaft Special chemiluminescent acridine derivatives
US5879953A (en) * 1988-02-20 1999-03-09 Hoechst Aktiengesellschaft Special chemiluminescent acridine derivatives and the use thereof in luminescence immunoassays
US6002007A (en) * 1988-02-20 1999-12-14 Dade Behring Marburg Gmbh Special chemiluminescent acridine derivatives and the use thereof in luminescence immunoassays
US6087476A (en) * 1988-03-24 2000-07-11 Igen International, Inc. Luminescent chimeric proteins
WO1991000511A1 (en) * 1989-06-24 1991-01-10 University Of Wales College Of Medicine Detecting or quantifying multiple analytes using labelling techniques

Also Published As

Publication number Publication date
GB2008247B (en) 1982-12-15

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Legal Events

Date Code Title Description
PE20 Patent expired after termination of 20 years

Effective date: 19981113