ES8203152A1 - Chemical luminescence amplification substrate system for immunochemistry - Google Patents

Chemical luminescence amplification substrate system for immunochemistry

Info

Publication number
ES8203152A1
ES8203152A1 ES497867A ES497867A ES8203152A1 ES 8203152 A1 ES8203152 A1 ES 8203152A1 ES 497867 A ES497867 A ES 497867A ES 497867 A ES497867 A ES 497867A ES 8203152 A1 ES8203152 A1 ES 8203152A1
Authority
ES
Spain
Prior art keywords
fluorescer
specie
biological
interest
immunochemistry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES497867A
Other languages
Spanish (es)
Other versions
ES497867A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alfa Wassermann Inc
Electro Nucleonics Inc
Original Assignee
Alfa Wassermann Inc
Electro Nucleonics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alfa Wassermann Inc, Electro Nucleonics Inc filed Critical Alfa Wassermann Inc
Publication of ES497867A0 publication Critical patent/ES497867A0/en
Publication of ES8203152A1 publication Critical patent/ES8203152A1/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

A system for the detection of a biological analyte of interest which comprises contacting a sample with a fluorescer which has been conjugated to an immunological specie specific to the biological analyte of interest, in the presence of an energy source which is capable of activating the fluorescer. A method for the qualitative and/or quantitative detection of a biological of interest is disclosed, which comprises: (1) labeling an immunological specie specific to the analyte of interest with a fluorescer material which is biologically compatible with such specie (b) contacting the fluorescer labeled specie and the biological of interest (c) separating the fluorescer labeled specie/biological complex (d) contacting the separated fluorescer labeled specie/biological complex of (c) with an energy source which is capable of activating the fluorescer label and (e) determining the presence of and/or measuring the quantum of chemiluminescent light emitted.
ES497867A 1979-12-19 1980-12-18 Chemical luminescence amplification substrate system for immunochemistry Expired ES8203152A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10525779A 1979-12-19 1979-12-19

Publications (2)

Publication Number Publication Date
ES497867A0 ES497867A0 (en) 1982-02-16
ES8203152A1 true ES8203152A1 (en) 1982-02-16

Family

ID=22304836

Family Applications (1)

Application Number Title Priority Date Filing Date
ES497867A Expired ES8203152A1 (en) 1979-12-19 1980-12-18 Chemical luminescence amplification substrate system for immunochemistry

Country Status (6)

Country Link
BE (1) BE886751A (en)
CA (1) CA1166133A (en)
ES (1) ES8203152A1 (en)
IT (1) IT1151457B (en)
WO (1) WO1981001883A1 (en)
ZA (1) ZA807274B (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4372745A (en) * 1979-12-19 1983-02-08 Electro-Nucleonics, Inc. Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer
CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
US4517303A (en) * 1982-10-20 1985-05-14 E. I. Du Pont De Nemours And Company Specific binding assays utilizing analyte-cytolysin conjugates
ES8607563A1 (en) * 1983-10-13 1986-06-01 Univ Georgia Res Found Bioluminescent immunoassays.
US4695554A (en) * 1984-02-14 1987-09-22 Becton Dickinson And Company Sac or liposome containing dye (sulforhodamine) for immunoassay
JPH0619348B2 (en) * 1984-06-15 1994-03-16 マスト イミユノシステムズ インコ−ポレ−テツド Attenuator that suppresses external light in luminescent specific binding assay
US4681859A (en) * 1984-09-21 1987-07-21 Ortho Diagnostic Systems Inc. Fluorescence polarization immunoassay for heavy antigens
US5221605A (en) * 1984-10-31 1993-06-22 Igen, Inc. Luminescent metal chelate labels and means for detection
US5238808A (en) * 1984-10-31 1993-08-24 Igen, Inc. Luminescent metal chelate labels and means for detection
US5310687A (en) * 1984-10-31 1994-05-10 Igen, Inc. Luminescent metal chelate labels and means for detection
US5422266A (en) * 1984-12-31 1995-06-06 University Of Georgia Research Foundation, Inc. Recombinant DNA vectors capable of expressing apoaequorin
FR2596048B1 (en) * 1986-03-18 1988-07-08 Sanofi Sa PHENYL- (METHOXY) PHENYL-PYRENYLMETHANE DERIVATIVES, PROCESS FOR OBTAINING SYNTHESIS INTERMEDIATES AND APPLICATION TO FLUORESCENT LABELING OF NUCLEOSIDES, NUCLEOTIDES AND POLYNUCLEOTIDES
US6451225B1 (en) 1986-04-30 2002-09-17 Igen International, Inc. Electrochemiluminescent reaction utilizing amine-derived reductant
JPH0737464B2 (en) * 1986-04-30 1995-04-26 イゲン，インコーポレーテッド Electro Chemiluminescence Atssei
US6165729A (en) * 1986-04-30 2000-12-26 Hyperion Catalysis International, Inc. Electrochemiluminescent reaction utilizing amine-derived reductant
US6702986B1 (en) 1988-04-29 2004-03-09 Igen International, Inc. Electrochemiluminescent reaction utilizing amine-derived reductant
AU2965200A (en) * 1999-01-11 2000-08-01 Lightsense Corporation Method and material for ratiometric fluorescent determination of analyte concentration

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3935074A (en) * 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US4199559A (en) * 1974-08-12 1980-04-22 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4169137A (en) * 1974-12-20 1979-09-25 Block Engineering, Inc. Antigen detecting reagents
US4011219A (en) * 1975-03-28 1977-03-08 Chugai Seiyaku Kabushiki Kaisha Phthalazine derivatives and salts thereof
US4018884A (en) * 1975-06-26 1977-04-19 Hoffmann-La Roche Inc. Fluorogenic materials and labeling techniques
US4181650A (en) * 1975-08-25 1980-01-01 Maier Charles L Jr Procedure for the assay of pharmacologically immunologically and biochemically active compounds in biological fluids
US4201763A (en) * 1975-10-09 1980-05-06 Bio-Rad Laboratories, Inc. Solid phase immunofluorescent assay method
US4036946A (en) * 1975-10-20 1977-07-19 Marcos Kleinerman Immunofluorometric method for measuring minute quantities of antigens, antibodies and other substances
US4020151A (en) * 1976-02-17 1977-04-26 International Diagnostic Technology, Inc. Method for quantitation of antigens or antibodies on a solid surface
US4160818A (en) * 1976-04-15 1979-07-10 Technicon Instruments Corporation Fluorimetric immunoassay for diphenylhydantoin
US4220450A (en) * 1978-04-05 1980-09-02 Syva Company Chemically induced fluorescence immunoassay
US4238195A (en) * 1979-01-18 1980-12-09 Miles Laboratories, Inc. Fluorescer-labeled specific binding assays

Also Published As

Publication number Publication date
WO1981001883A1 (en) 1981-07-09
ZA807274B (en) 1982-06-30
BE886751A (en) 1981-04-16
ES497867A0 (en) 1982-02-16
IT1151457B (en) 1986-12-17
IT8026043A0 (en) 1980-11-17
CA1166133A (en) 1984-04-24

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