ES8400773A1 - Glucose oxidase immunohistochemical detection of antinuclear antibodies. - Google Patents

Glucose oxidase immunohistochemical detection of antinuclear antibodies.

Info

Publication number
ES8400773A1
ES8400773A1 ES515350A ES515350A ES8400773A1 ES 8400773 A1 ES8400773 A1 ES 8400773A1 ES 515350 A ES515350 A ES 515350A ES 515350 A ES515350 A ES 515350A ES 8400773 A1 ES8400773 A1 ES 8400773A1
Authority
ES
Spain
Prior art keywords
antigen source
glucose oxidase
immunohistochemical detection
contacting
stabilized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES515350A
Other languages
Spanish (es)
Other versions
ES515350A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ICL SCIENT
Original Assignee
ICL SCIENT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ICL SCIENT filed Critical ICL SCIENT
Publication of ES8400773A1 publication Critical patent/ES8400773A1/en
Publication of ES515350A0 publication Critical patent/ES515350A0/en
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G01N33/567—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds utilising isolate of tissue or organ as binding agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/552—Glass or silica

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

For use in the immunohistochemical detection of antigens and related antibodies in a test serum, an antigen source fixed on a support and stabilized by dehydration treatment with an organic solvent mixture, such as aceton methanol, or mixtures thereof. The support is stored under nitrogen in a sealed polyethylene bag. Further, an immunoenzymatic method for the detection of biological components for a test sample, the method comprising the steps of: contacting the stabilized antigen source with the test sample, contacting the antigen source with an antibody conjugated with glucose oxidase and reactive with the component incubating the antigen source with a solution containing glucose and a chromagenic mixture and analyzing the antigen source for the presence of color, preferably with a light microscope.
ES515350A 1981-08-31 1982-08-30 "AN IMMUNOENZYMATIC PROCEDURE FOR THE DETECTION OF A COMPONENT OF A TEST SAMPLE". Granted ES515350A0 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29811281A 1981-08-31 1981-08-31

Publications (2)

Publication Number Publication Date
ES8400773A1 true ES8400773A1 (en) 1983-11-01
ES515350A0 ES515350A0 (en) 1983-11-01

Family

ID=23149085

Family Applications (2)

Application Number Title Priority Date Filing Date
ES515350A Granted ES515350A0 (en) 1981-08-31 1982-08-30 "AN IMMUNOENZYMATIC PROCEDURE FOR THE DETECTION OF A COMPONENT OF A TEST SAMPLE".
ES524183A Expired ES8500999A1 (en) 1981-08-31 1983-07-16 Glucose oxidase immunohistochemical detection of antinuclear antibodies.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES524183A Expired ES8500999A1 (en) 1981-08-31 1983-07-16 Glucose oxidase immunohistochemical detection of antinuclear antibodies.

Country Status (5)

Country Link
EP (1) EP0091911A4 (en)
CA (1) CA1206092A (en)
ES (2) ES515350A0 (en)
IT (1) IT1153188B (en)
WO (1) WO1983000877A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4487830A (en) * 1982-05-14 1984-12-11 American Hoechst Corporation Enzyme/immunofluorescent assay for autoantibodies
US4588682A (en) * 1982-12-13 1986-05-13 Integrated Genetics, Inc. Binding nucleic acid to a support
US4814269A (en) * 1985-03-29 1989-03-21 Cenfold Holdings S.A. Diagnostic testing for antibodies against microorganisms
EP0206779A1 (en) * 1985-06-21 1986-12-30 Modern Diagnostics, Inc. Detecting antinuclear antibody
JPS62255869A (en) * 1986-04-08 1987-11-07 フイツトテイカー　バイオプロダクツ　インコーポレーテツド Method and substrate for immunofluorescent microscopy
CA1313818C (en) * 1987-06-03 1993-02-23 Ross Leon Coppel Nuclear antigen la
EP0335293A3 (en) * 1988-03-30 1990-09-12 Hoechst Japan Limited A method of preparing an analytical element for the determination of anti-nuclear antibodies
CN100504388C (en) 2004-02-20 2009-06-24 北京新景安太医疗技术服务有限公司 A method for diagnosing immune recurrent spontaneous abortion and method for treating and monitoring
EP1719516B1 (en) 2004-02-20 2009-09-02 Beijing Xinjing Antai Medical And Technology Service Limited Corp. A pharmaceutical composition used for treating recurrent spontaneous abortion and method thereof
US7763418B2 (en) * 2005-07-05 2010-07-27 Cytoskeleton, Inc. Detection of Rho proteins

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3146163A (en) * 1962-01-23 1964-08-25 John H Brewer Apparatus for separating certain components from blood
NL154600B (en) * 1971-02-10 1977-09-15 Organon Nv METHOD FOR THE DETERMINATION AND DETERMINATION OF SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES.
US3997657A (en) * 1973-10-15 1976-12-14 American Cyanamid Company Dry slide reagent employed in immunofluorescent test for detection of human antinuclear factor
US3876504A (en) * 1974-06-10 1975-04-08 Early Warning Co Procedure for determination of antigens and antibodies and articles for use therewith
US3979509A (en) * 1974-09-03 1976-09-07 General Electric Company Opaque layer method for detecting biological particles
DE2836362A1 (en) * 1978-08-19 1980-03-06 Behringwerke Ag DIAGNOSTIC MEANS

Also Published As

Publication number Publication date
IT1153188B (en) 1987-01-14
EP0091911A1 (en) 1983-10-26
ES524183A0 (en) 1984-11-01
ES8500999A1 (en) 1984-11-01
WO1983000877A1 (en) 1983-03-17
IT8223075A0 (en) 1982-08-31
CA1206092A (en) 1986-06-17
EP0091911A4 (en) 1984-04-06
ES515350A0 (en) 1983-11-01

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