ES2110408T3 - Ensayo para la fosfatasa alcalina osea. - Google Patents

Ensayo para la fosfatasa alcalina osea.

Info

Publication number
ES2110408T3
ES2110408T3 ES90300959T ES90300959T ES2110408T3 ES 2110408 T3 ES2110408 T3 ES 2110408T3 ES 90300959 T ES90300959 T ES 90300959T ES 90300959 T ES90300959 T ES 90300959T ES 2110408 T3 ES2110408 T3 ES 2110408T3
Authority
ES
Spain
Prior art keywords
bap
antibody
amount
fluid
measured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90300959T
Other languages
English (en)
Inventor
Craig Stevens Hill
Robert Lewis Wolfert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hybritech Inc
Original Assignee
Hybritech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybritech Inc filed Critical Hybritech Inc
Application granted granted Critical
Publication of ES2110408T3 publication Critical patent/ES2110408T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Abstract

UN PROCEDIMIENTO PARA LA DETERMINACION DE LA PRESENCIA O CONCENTRACION DE ENZIMA DE FOSFATASA ALCALINA DE HUESOS (BAP) EN UN FLUIDO, QUE COMPRENDE: (A)EL CONTACTO DE UNA MUESTRA DEL FLUIDO CON UN PRIMER ANTICUERPO MONOCLONAL PARA BAP, EN EL QUE EL PRIMER ANTICUERPO MONOCLONAL ESTA UNIDO A UN PORTADOR SOLIDO INSOLUBLE EN EL FLUIDO, CON OBJETO DE OBTENER UN COMPLEJO INSOLUBLE ENTRE EL PRIMER ANTICUERPO MONOCLONAL Y BAP; (B)LA SEPARACION DE LA MUESTRA DEL FLUIDO CONTENIENDO BAP NO REACCIONADO PROCEDENTE DEL COMPLEJO INSOLUBLE DEL PRIMER ANTICUERPO MONOCLONAL Y BAP; (C)LA REACCION DE UNA CANTIDAD MEDIDA DE UN SEGUNDO ETIQUETADO ANTICUERPO MONOCLONAL A BAP, CUYO ANTICUERPO ES SOLUBLE EN EL FLUIDO, CON EL COMPLEJO INSOLUBLE DEL PRIMER ANTICUERPO MONOCLONAL Y BAP, PARA OBTENER UN COMPLEJO INSOLUBLE DEL PRIMER ANTICUERPO MONOCLONAL, BAP, Y UN SEGUNDO ETIQUETADO ANTICUERPO: (D)LA SEPARACION DEL PORTADOR SOLIDO, DEL SEGUNDO ETIQUETADO ANTICUERPO, NO REACCIONADO; (E)LA MEDICION, BIEN DE LA CANTIDAD DEL SEGUNDO ETIQUETADO ANTICUERPO, ASOCIADO CON EL PORTADOR SOLIDO, BIEN LA CANTIDAD DEL SEGUNDO ETIQUETADO ANTICUERPO NO REACCIONADO; (F)LA RELACION DE CANTIDAD DEL SEGUNDO ETIQUETADO ANTICUERPO MEDIDO CON LA CANTIDAD DEL ANTICUERPO ETIQUETADO, MEDIDO PARA UNA MUESTRA DE CONTROL PREPARADA DE CONFORMIDAD CON LAS FASES (A) A (E), CONOCIENDOSE QUE LA CITADA MUESTRA DE CONTROL ESTARA LIBRE DE BAP, PARA DETERMINAR LA PRESENCIA DE BAP EN LA CITADA MUESTRA DE FLUIDO, O RELACIONANDO LA CANTIDAD MEDIDA DE ANTICUERPO ETIQUETADO PARA LA MUESTRA DE FLUIDO, CON LA CANTIDAD DE ANTICUERPO ETIQUETADO, MEDIDO PARA MUESTRAS QUE CONTIENEN UNA CANTIDAD CONOCIDA DE BAP, PREPARADA DE CONFORMIDAD CON LAS FASES (A) A (E), PARA DETERMINAR LA CONCENTRACION DE BAP EN LA MUESTRA DE FLUIDO; EN EL QUE AMBOS ANTICUERPOS MONOCLONALES UTILIZADOS SON ALTAMENTE ESPECIFICOS PARA BAP, ESPECIALMENTE EN PRESENCIA DE LAP.
ES90300959T 1989-01-31 1990-01-30 Ensayo para la fosfatasa alcalina osea. Expired - Lifetime ES2110408T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US30452189A 1989-01-31 1989-01-31

Publications (1)

Publication Number Publication Date
ES2110408T3 true ES2110408T3 (es) 1998-02-16

Family

ID=23176884

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90300959T Expired - Lifetime ES2110408T3 (es) 1989-01-31 1990-01-30 Ensayo para la fosfatasa alcalina osea.

Country Status (10)

Country Link
US (2) US5525473A (es)
EP (1) EP0381450B1 (es)
JP (1) JP3055789B2 (es)
AT (1) ATE160021T1 (es)
AU (1) AU635518B2 (es)
CA (1) CA2008304C (es)
DE (1) DE69031662T2 (es)
DK (1) DK0381450T3 (es)
ES (1) ES2110408T3 (es)
GR (1) GR3025816T3 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2008304C (en) * 1989-01-31 2001-03-27 Craig S. Hill Assay for bone alkaline phosphatase
US6201109B1 (en) 1993-01-13 2001-03-13 Dade Behring Marburg Gmbh Assay for bone alkaline phosphatase
US6303325B1 (en) 1998-05-29 2001-10-16 Dade Behring Inc. Method for detecting analytes
GB9812290D0 (en) * 1998-06-09 1998-08-05 Univ Nottingham Back pain diagnosis
SE0103072D0 (sv) * 2001-09-17 2001-09-17 Pharmacia Diagnostics Ab Multi-analyte assay device with multi-spot detection zone
JP4517353B2 (ja) * 2004-11-02 2010-08-04 日東紡績株式会社 ＩｇＡ結合性アルカリ性ホスファターゼ６の測定方法およびそれに用いるキット
JP2010526766A (ja) * 2007-02-02 2010-08-05 ノバルティス アーゲー 骨関連障害を処置するためのスクレロスチン結合パートナーのモジュレーター
US8658166B2 (en) 2011-11-08 2014-02-25 Caldera Health Limited Methods and materials for the diagnosis of prostate cancers
US20130116131A1 (en) 2011-11-08 2013-05-09 Caldera Health Limited Methods and materials for the diagnosis of prostate cancers
US8632971B2 (en) 2011-11-08 2014-01-21 Caldera Health Limited Methods and materials for determining the efficacy of prostate cancer therapies
US8658164B2 (en) 2011-11-08 2014-02-25 Caldera Health Limited Methods and materials for the diagnosis of prostate cancers
CN103412120A (zh) * 2013-09-03 2013-11-27 天津朗赛生物科技有限公司 人体血液中铁蛋白和骨碱性磷酸酶联合检测试纸及其制备方法
CN103412123A (zh) * 2013-09-03 2013-11-27 天津朗赛生物科技有限公司 骨碱性磷酸酶检测试纸及其制备方法
CN103675282B (zh) * 2013-11-27 2016-01-20 新昌县美迪生物科技有限公司 骨源性碱性磷酸酶定量检测试剂盒及其用途
CN114088626B (zh) * 2021-11-16 2022-08-26 国家食品安全风险评估中心 一种鉴定巴氏杀菌乳的方法及检测系统

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302536A (en) * 1978-08-15 1981-11-24 Longenecker Robert W Colorimetric immunoassay process
US4376110A (en) * 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
DE3420926A1 (de) * 1984-06-05 1985-12-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zur differenzierten bestimmung von isoenzymen der alkalischen phosphatase sowie hierzu geeigneter monoklonaler antikoerper
US4740468A (en) * 1985-02-14 1988-04-26 Syntex (U.S.A.) Inc. Concentrating immunochemical test device and method
TW203120B (es) * 1985-10-04 1993-04-01 Abbott Lab
US4912033A (en) * 1985-11-14 1990-03-27 Washington University Creatine kinase MB determination method
US4916056A (en) * 1986-02-18 1990-04-10 Abbott Laboratories Solid-phase analytical device and method for using same
US5087573A (en) * 1988-03-02 1992-02-11 University Of Kansas Monoclonal antibody against bone alkaline phosphatase
CA2008304C (en) * 1989-01-31 2001-03-27 Craig S. Hill Assay for bone alkaline phosphatase

Also Published As

Publication number Publication date
EP0381450A2 (en) 1990-08-08
EP0381450B1 (en) 1997-11-05
DE69031662D1 (de) 1997-12-11
JP3055789B2 (ja) 2000-06-26
US5589574A (en) 1996-12-31
ATE160021T1 (de) 1997-11-15
EP0381450A3 (en) 1991-09-18
GR3025816T3 (en) 1998-03-31
DK0381450T3 (da) 1998-07-27
AU4881890A (en) 1990-08-09
CA2008304C (en) 2001-03-27
AU635518B2 (en) 1993-03-25
DE69031662T2 (de) 1998-03-05
US5525473A (en) 1996-06-11
JPH02259570A (ja) 1990-10-22
CA2008304A1 (en) 1990-07-31

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