MY101455A - Homogeneous fluorescence immunoassay using a light absorbing material. - Google Patents

Homogeneous fluorescence immunoassay using a light absorbing material.

Info

Publication number
MY101455A
MY101455A MYPI87002121A MYPI19872121A MY101455A MY 101455 A MY101455 A MY 101455A MY PI87002121 A MYPI87002121 A MY PI87002121A MY PI19872121 A MYPI19872121 A MY PI19872121A MY 101455 A MY101455 A MY 101455A
Authority
MY
Malaysia
Prior art keywords
analyte
tracer
absorbing material
light absorbing
bound
Prior art date
Application number
MYPI87002121A
Inventor
A Baffi Robert
B Wagner Daniel
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Publication of MY101455A publication Critical patent/MY101455A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A METHOD FOR SEPARATION-FREE SOLID PHASE IMMUNOASSAY OF AN ANALYTE INCLUDES CONTACTING AN ANTI-ANALYTE ATTACHED TO THE SURFACE OF A SOLID SUPPORT WITH THE ANALYTE, A LIGHT ABSORBING MATERIAL AND A FLUORESCENT TRACER FOR THE ANALYTE. THE RESULTING MIXTURE IS INCUBATED. THE METHOD INCLUDES APPLYING EXCITATION LIGHT TO THE MIXTURE AND TIME RESOLVED MEASUREMENT OF FLUORESCENCE EMISSION FROM THE TRACER. ALL EXCITATION LIGHT AND FLUORESCENCE EMISSION ARE ABSORBED BY THE LIGHT ABSORBING MATERIAL EXCEPT THAT ABSORBED AND EMITTED BY THE TRACER BOUND TO THE ANTI-ANALYTE WHEREBY THE ONLY FLUORESCENCE EMISSION DETECTED IS FROM THE BOUND TRACER. SINCE FREE TRACER IN THE FLUID PHASE OF THE ASSAY MEDIUM DOES NOT EMIT FLUORESCENCE, SEPARATION OF THE BOUND AND FREE FRACTIONS IS UNNECESSARY. THE INVENTION INCLUDES A KIT OF MATERIALS USEFUL IN PERFORMING AN IMMUNOASSAY IN ACCORDANCE WITH THE METHOD OF THE INVENTION.
MYPI87002121A 1985-02-11 1987-09-29 Homogeneous fluorescence immunoassay using a light absorbing material. MY101455A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/700,578 US4680275A (en) 1985-02-11 1985-02-11 Homogeneous fluorescence immunoassay using a light absorbing material

Publications (1)

Publication Number Publication Date
MY101455A true MY101455A (en) 1991-11-18

Family

ID=24814048

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI87002121A MY101455A (en) 1985-02-11 1987-09-29 Homogeneous fluorescence immunoassay using a light absorbing material.

Country Status (9)

Country Link
US (1) US4680275A (en)
EP (1) EP0191575B1 (en)
JP (1) JPS61186856A (en)
AU (1) AU580361B2 (en)
CA (1) CA1253798A (en)
DE (1) DE3671300D1 (en)
DK (1) DK164840C (en)
FI (1) FI81680C (en)
MY (1) MY101455A (en)

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US4977077A (en) * 1984-10-24 1990-12-11 Bioprobe International Integrated solid-phase immunoassay
GB8622855D0 (en) * 1986-09-23 1986-10-29 Ekins R P Determining biological substance
US4954435A (en) * 1987-01-12 1990-09-04 Becton, Dickinson And Company Indirect colorimetric detection of an analyte in a sample using ratio of light signals
EP0290269A3 (en) * 1987-05-06 1989-08-09 Cyberfluor Inc. Immunoassay methods and reagents and methods for producing the latter
US4837162A (en) * 1987-06-05 1989-06-06 Pall Corporation Non-fluorescing, non-reflective polyamide for use in diagnostic testing
SE8703682L (en) * 1987-09-24 1989-03-25 Wallac Oy HOMOGENIC DETERMINATION METHOD USING AFFINITY REACTIONS
US5089384A (en) * 1988-11-04 1992-02-18 Amoco Corporation Method and apparatus for selective cell destruction using amplified immunofluorescence
US5198340A (en) * 1991-01-17 1993-03-30 Genentech, Inc. Assay for free igf-i, igf-ii, and gh levels in body fluids
US5210412A (en) * 1991-01-31 1993-05-11 Wayne State University Method for analyzing an organic sample
US5424414A (en) * 1991-12-17 1995-06-13 Abbott Laboratories Haptens, tracers, immunogens and antibodies for 3-phenyl-1-adamantaneacetic acids
US5616505A (en) * 1992-03-27 1997-04-01 Abbott Laboratories Haptens tracers, immunogens and antibodies for 3-phenyl-1-adamantaneacetic acids
DE4210970C2 (en) * 1992-04-02 1996-10-17 Markus Dipl Chem Sauer Process for the simultaneous optical qualitative and quantitative detection of different molecules of a mixture marked with fluorochromes or fluorogens by means of laser spectroscopy
US5312922A (en) * 1992-04-06 1994-05-17 Nordion International Inc. Europium and terbium chelators for time-resolved fluorometric assays
US5294799A (en) * 1993-02-01 1994-03-15 Aslund Nils R D Apparatus for quantitative imaging of multiple fluorophores
ES2153383T3 (en) * 1993-06-10 2001-03-01 Abbott Lab HAPTENS, TRAZERS, IMMUNOGENS AND ANTIBODIES FOR 3-PHENYLENE-1-ADAMANTANOACETIC ACIDS.
US5599668A (en) * 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
US5856194A (en) 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US5795784A (en) 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6171295B1 (en) * 1999-01-20 2001-01-09 Scimed Life Systems, Inc. Intravascular catheter with composite reinforcement
DE19903576C2 (en) 1999-01-29 2001-02-22 Bodenseewerk Perkin Elmer Co Quantitative determination of analytes in a heterogeneous system
CN1227532C (en) * 1999-09-29 2005-11-16 科学技术振兴事业团 High sensitive immunoassay
US6702453B2 (en) 2001-10-26 2004-03-09 Birchwood Lighting, Inc. Flexible light fixture
US20050112703A1 (en) * 2003-11-21 2005-05-26 Kimberly-Clark Worldwide, Inc. Membrane-based lateral flow assay devices that utilize phosphorescent detection
EP1908044B1 (en) * 2005-06-29 2011-04-06 Eidgenössische Technische Hochschule Zürich Unique label for identification or security system
GB2474224A (en) * 2009-07-07 2011-04-13 Toximet Ltd Devices and methods using fluorescent polymers in solid phase extraction
WO2011151250A1 (en) * 2010-05-31 2011-12-08 Boehringer Ingelheim Microparts Gmbh Method and device for optical examination
AU2013234281B2 (en) 2012-03-16 2016-06-23 Stat-Diagnostica & Innovation, S.L. A test cartridge with integrated transfer module
CN104520690A (en) * 2012-05-25 2015-04-15 珠海恺瑞生物科技有限公司(中国) Apparatuses and methods for conducting binding assays
RU2710262C1 (en) * 2019-08-01 2019-12-25 Закрытое акционерное общество "ИММУНОСКРИН" (ЗАО "ИММУНОСКРИН") Biological analysis method

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US3939350A (en) * 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US4058732A (en) * 1975-06-30 1977-11-15 Analytical Radiation Corporation Method and apparatus for improved analytical fluorescent spectroscopy
US4341957A (en) * 1975-11-26 1982-07-27 Analytical Radiation Corporation Fluorescent antibody composition for immunofluorometric assay
US4283382A (en) * 1977-12-28 1981-08-11 Eastman Kodak Company Fluorescent labels comprising rare earth chelates
CA1111762A (en) * 1977-12-28 1981-11-03 David S. Frank Fluorescent rare earth chelate in polymeric latex particles
US4271139A (en) * 1978-03-27 1981-06-02 Hiram Hart Scintillation proximity assay
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US4318707A (en) * 1978-11-24 1982-03-09 Syva Company Macromolecular fluorescent quencher particle in specific receptor assays
US4256834A (en) * 1979-04-09 1981-03-17 Syva Company Fluorescent scavenger particle immunoassay
IL64574A (en) * 1981-04-27 1986-01-31 Syva Co Method for determining the presence of an analyte by determination of the amount of fluorescence in competitive protein binding assays
US4582809A (en) * 1982-06-14 1986-04-15 Myron J. Block Apparatus including optical fiber for fluorescence immunoassay
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Also Published As

Publication number Publication date
FI855019A (en) 1986-08-12
US4680275A (en) 1987-07-14
EP0191575B1 (en) 1990-05-16
DE3671300D1 (en) 1990-06-21
CA1253798A (en) 1989-05-09
DK65386D0 (en) 1986-02-11
JPS61186856A (en) 1986-08-20
DK65386A (en) 1986-08-12
DK164840C (en) 1993-01-04
AU5102585A (en) 1986-08-14
FI81680B (en) 1990-07-31
EP0191575A1 (en) 1986-08-20
AU580361B2 (en) 1989-01-12
DK164840B (en) 1992-08-24
JPH0476580B2 (en) 1992-12-04
FI855019A0 (en) 1985-12-17
FI81680C (en) 1990-11-12

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