ES2072900T3 - Expresion elevada de proteinas en levadura. - Google Patents
Expresion elevada de proteinas en levadura.Info
- Publication number
- ES2072900T3 ES2072900T3 ES89304297T ES89304297T ES2072900T3 ES 2072900 T3 ES2072900 T3 ES 2072900T3 ES 89304297 T ES89304297 T ES 89304297T ES 89304297 T ES89304297 T ES 89304297T ES 2072900 T3 ES2072900 T3 ES 2072900T3
- Authority
- ES
- Spain
- Prior art keywords
- yeast
- high expression
- yeast proteins
- guest
- refers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108010058643 Fungal Proteins Proteins 0.000 title 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 2
- 102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 abstract 1
- 101710193111 All-trans-retinol dehydrogenase [NAD(+)] ADH4 Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/50—Fibroblast growth factor [FGF]
- C07K14/503—Fibroblast growth factor [FGF] basic FGF [bFGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
LA INVENCION SE REFIERE A UN METODO PARA EXPRESAR PROTEINAS NO DE LEVADURAS BAJO EL CONTROL DE SECUENCIAS REGULADORAS DE ADH2 EN UNA LEVADURA HUESPED QUE TIENE EXPRESION MEJORADA DE ADRI. TAMBIEN SE REFIERE LA INVENCION A LEVADURAS HUESPED TRANSFORMADAS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US19086888A | 1988-05-06 | 1988-05-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2072900T3 true ES2072900T3 (es) | 1995-08-01 |
Family
ID=22703135
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES89304297T Expired - Lifetime ES2072900T3 (es) | 1988-05-06 | 1989-04-28 | Expresion elevada de proteinas en levadura. |
Country Status (8)
Country | Link |
---|---|
US (1) | US6183985B1 (es) |
EP (1) | EP0340986B1 (es) |
JP (4) | JP3074174B2 (es) |
AT (1) | ATE123521T1 (es) |
CA (1) | CA1324969C (es) |
DE (1) | DE68922932T2 (es) |
DK (1) | DK175898B1 (es) |
ES (1) | ES2072900T3 (es) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0801682B1 (en) | 1995-02-09 | 2001-10-24 | Novartis AG | Process for the production of proteins |
DE19544233A1 (de) * | 1995-11-28 | 1997-06-05 | Hoechst Ag | Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten |
BR0212649A (pt) * | 2001-09-20 | 2004-08-24 | Toyota Motor Co Ltd | Sistema para alcançar elevada expressão gênica |
JP2007519422A (ja) | 2004-02-02 | 2007-07-19 | アンブレツクス・インコーポレイテツド | 修飾されたヒト四螺旋バンドルポリペプチド及びそれらの使用 |
MXPA06014684A (es) * | 2004-06-18 | 2007-02-12 | Ambrx Inc | Novedosos polipeptidos de enlace antigeno y sus usos. |
AU2005327906B2 (en) * | 2004-07-21 | 2010-05-13 | Ambrx, Inc. | Biosynthetic polypeptides utilizing non-naturally encoded amino acids |
BRPI0519430A2 (pt) | 2004-12-22 | 2009-02-10 | Ambrx Inc | hormânio do crescimento humano modificado |
AU2005322019B2 (en) | 2004-12-22 | 2010-08-26 | Ambrx, Inc. | Formulations of human growth hormone comprising a non-naturally encoded amino acid |
WO2006069246A2 (en) | 2004-12-22 | 2006-06-29 | Ambrx, Inc. | Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides |
SG161210A1 (en) * | 2004-12-22 | 2010-05-27 | Ambrx Inc | Methods for expression and purification of recombinant human growth hormone |
ATE541934T1 (de) * | 2004-12-22 | 2012-02-15 | Ambrx Inc | Zusammensetzungen von aminoacyl-trna-synthetase und verwendungen davon |
KR20080026135A (ko) * | 2005-06-03 | 2008-03-24 | 암브룩스, 인코포레이티드 | 개선된 인간 인터페론 분자 및 이의 용도 |
DE102005029170A1 (de) * | 2005-06-23 | 2006-12-28 | Consortium für elektrochemische Industrie GmbH | System zur Genexpression in Hefen der Gattung Sporidiobolus |
MX2008002149A (es) | 2005-08-18 | 2008-04-22 | Ambrx Inc | Composiciones de arnt y sus usos. |
PT1954710E (pt) * | 2005-11-08 | 2011-06-07 | Ambrx Inc | Aceleradores para a modificação de aminoácidos nãonaturais e polipeptídeos de aminoácidos não-naturais |
EP2339014B1 (en) | 2005-11-16 | 2015-05-27 | Ambrx, Inc. | Methods and compositions comprising non-natural amino acids |
CA2630220C (en) | 2005-11-22 | 2020-10-13 | Doris Coit | Norovirus and sapovirus antigens |
US8420792B2 (en) * | 2006-09-08 | 2013-04-16 | Ambrx, Inc. | Suppressor tRNA transcription in vertebrate cells |
EP2069396B1 (en) * | 2006-09-08 | 2015-10-21 | Ambrx, Inc. | Modified human plasma polypeptide or fc scaffolds and their uses |
NZ574721A (en) * | 2006-09-08 | 2012-02-24 | Ambrx Inc | Hybrid suppressor trna for vertebrate cells |
DK2106447T3 (da) * | 2007-01-26 | 2013-06-24 | Novozymes As | Fremgangsmåde til methanoluafhængig induktion fra methanolinducerbare promotorer i Pichia |
BRPI0809583B1 (pt) | 2007-03-30 | 2022-02-22 | Ambrx, Inc | Polipeptídeo fgf-21 modificado, composição compreendendo o mesmo, método para produzir o referido polipetídeo fgf-21 e célula compreendendo um polinucleotídeo |
CA2685596A1 (en) | 2007-05-02 | 2008-11-13 | Ambrx, Inc. | Modified interferon beta polypeptides and their uses |
NZ603812A (en) | 2007-11-20 | 2014-06-27 | Ambrx Inc | Modified insulin polypeptides and their uses |
SG188143A1 (en) | 2008-02-08 | 2013-03-28 | Ambrx Inc | Modified leptin polypeptides and their uses |
EP2300615A2 (en) * | 2008-07-11 | 2011-03-30 | Novozymes A/S | Pichia pastoris das promoter variants |
NZ591235A (en) | 2008-07-23 | 2012-07-27 | Ambrx Inc | Modified bovine g-csf polypeptides comprising non natural amino acid and their uses treating infections such as mastitis |
PL2342223T3 (pl) | 2008-09-26 | 2017-09-29 | Ambrx, Inc. | Zmodyfikowane polipeptydy zwierzęcej erytropoetyny i ich zastosowania |
TR201802361T4 (tr) | 2008-09-26 | 2018-03-21 | Ambrx Inc | Doğal olmayan amino asit replikasyonuna bağımlı mikroorganizmalar ve aşılar. |
CN104017063A (zh) | 2009-12-21 | 2014-09-03 | Ambrx公司 | 经过修饰的猪促生长素多肽和其用途 |
NZ600361A (en) | 2009-12-21 | 2014-06-27 | Ambrx Inc | Modified bovine somatotropin polypeptides and their uses |
CA2804501C (en) | 2010-07-06 | 2021-01-26 | Novartis Ag | Noro virus derived immunogenic compositions and methods |
US9567386B2 (en) | 2010-08-17 | 2017-02-14 | Ambrx, Inc. | Therapeutic uses of modified relaxin polypeptides |
MY162837A (en) | 2010-08-17 | 2017-07-31 | Ambrx Inc | Modified relaxin polypeptides and their uses |
AR083006A1 (es) | 2010-09-23 | 2013-01-23 | Lilly Co Eli | Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas |
UY36370A (es) | 2014-10-24 | 2016-04-29 | Bristol Myers Squibb Company Una Corporación Del Estado De Delaware | Polipéptidos fgf-21 modificados y sus usos |
ES2963839T3 (es) | 2017-02-08 | 2024-04-02 | Bristol Myers Squibb Co | Polipéptidos de relaxina modificados que comprenden un potenciador farmacocinético y usos de los mismos |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4880734A (en) * | 1984-05-11 | 1989-11-14 | Chiron Corporation | Eukaryotic regulatable transcription |
EP0196056B1 (en) * | 1985-03-28 | 1991-05-22 | Chiron Corporation | Improved expression using fused genes providing for protein product |
EP0234871A3 (en) | 1986-02-28 | 1988-10-12 | Merck & Co. Inc. | Regulatable expression of recombinant proteins in yeast |
US5068185A (en) | 1986-07-10 | 1991-11-26 | Merck & Co., Inc. | Trains of yeast for the expression of heterologous genes |
-
1989
- 1989-04-21 CA CA000597461A patent/CA1324969C/en not_active Expired - Lifetime
- 1989-04-28 ES ES89304297T patent/ES2072900T3/es not_active Expired - Lifetime
- 1989-04-28 DE DE68922932T patent/DE68922932T2/de not_active Expired - Lifetime
- 1989-04-28 EP EP89304297A patent/EP0340986B1/en not_active Expired - Lifetime
- 1989-04-28 AT AT89304297T patent/ATE123521T1/de not_active IP Right Cessation
- 1989-05-02 JP JP01113585A patent/JP3074174B2/ja not_active Expired - Lifetime
- 1989-05-05 DK DK198902215A patent/DK175898B1/da not_active IP Right Cessation
-
1995
- 1995-03-01 US US08/397,553 patent/US6183985B1/en not_active Expired - Lifetime
-
2000
- 2000-01-12 JP JP2000004098A patent/JP3484386B2/ja not_active Expired - Lifetime
-
2002
- 2002-08-14 JP JP2002236635A patent/JP3561513B2/ja not_active Expired - Fee Related
-
2003
- 2003-12-10 JP JP2003412625A patent/JP2004081222A/ja not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
JP2000300277A (ja) | 2000-10-31 |
JP3074174B2 (ja) | 2000-08-07 |
DK221589D0 (da) | 1989-05-05 |
EP0340986A3 (en) | 1991-07-31 |
DE68922932T2 (de) | 1995-10-19 |
DK221589A (da) | 1989-11-07 |
DE68922932D1 (de) | 1995-07-13 |
EP0340986A2 (en) | 1989-11-08 |
JP3561513B2 (ja) | 2004-09-02 |
ATE123521T1 (de) | 1995-06-15 |
JP2003116525A (ja) | 2003-04-22 |
US6183985B1 (en) | 2001-02-06 |
JP3484386B2 (ja) | 2004-01-06 |
JPH02104291A (ja) | 1990-04-17 |
EP0340986B1 (en) | 1995-06-07 |
CA1324969C (en) | 1993-12-07 |
JP2004081222A (ja) | 2004-03-18 |
DK175898B1 (da) | 2005-05-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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