ES2058344T3 - Inmunoensayo de operacion positiva. - Google Patents
Inmunoensayo de operacion positiva.Info
- Publication number
- ES2058344T3 ES2058344T3 ES89100758T ES89100758T ES2058344T3 ES 2058344 T3 ES2058344 T3 ES 2058344T3 ES 89100758 T ES89100758 T ES 89100758T ES 89100758 T ES89100758 T ES 89100758T ES 2058344 T3 ES2058344 T3 ES 2058344T3
- Authority
- ES
- Spain
- Prior art keywords
- analyte
- antibody
- limit
- amount
- level
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/743—Steroid hormones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
Abstract
UN PROCEDIMIENTO DE ENSAYO QUE ES ESPECIALMENTE UTILIZABLE PARA DETECTAR Y/O DETERMINAR LA PRESENCIA DE NIVELES LIMITE DELIGANDOS ANALITO EN FLUIDOS BIOLOGICOS. EN UN ASPECTO PARTICULARIZADO Y ESPECIALIZADO LA INVENCION SE DIRIGE A PROCEDIMIENTOS PARA DETECTAR Y/O DETERMINAR NIVELES LIMITE DE METABOLITOS DE HORMONAS, TALES COMO PREGNANODIOL-3-GLUCURONIDA (P3G) Y ESTRONA-3-GLUCURONIDA (E13G) EN ORINA HUMANA. EL ENSAYO CONSISTE EN PONER EN CONTACTO UNA MUESTRA QUE CONTIENE EL ANALITO CON UNA CANTIDAD CONOCIDA DE UN ANTICUERPO SUYO Y CON UNA CANTIDAD CALIBRADA DEL ANALITO MISMO QUE ESTA CONJUGADO A DICHO SOPORTE SOLIDO. CUANDO EL NIVEL DEL ANALITO EN LA MUESTRA EXCEDE UN NIVEL LIMITE, TAL COMO 5 (MU)G/ML PARA P3G, EL ANTICUERPO SERA INSUFICIENTE PARA BLOQUEAR TODO EL ANALITO CORRESPONDIENTE EN EL SOPORTE SOLIDO. ASI, POR ADICION DE ANTICUERPO MARCADO AL SISTEMA DE ENSAYO, SE LLEGA A UNIR AL SOPORTE UN PRODUCTO DE INMUNORREACCION DETECTABLE PARA INDICAR QUE LA CANTIDAD DE ANALITO EN LA MUESTRA EXCEDE EL NIVEL LIMITE. POR OTRA PARTE, SI EL NIVEL DEL ANALITO EN LA MUESTRA ES INFERIOR A LA CANTIDAD LIMITE, EL ANTICUERPO LIBRE SERA SUFICIENTE PARA BLOQUEAR TODO EL ANALITO CORRESPONDIENTE EN EL SOPORTE SOLIDO, EVITANDO QUE EL ANTICUERPO MARCADO FORME UN PRODUCTO DE INMUNORREACCION DETECTABLE SOBRE EL SOPORTE, Y ASI NO APARECE SEÑAL.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/153,081 US4952517A (en) | 1988-02-08 | 1988-02-08 | Positive step immunoassay |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2058344T3 true ES2058344T3 (es) | 1994-11-01 |
Family
ID=22545697
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES89100758T Expired - Lifetime ES2058344T3 (es) | 1988-02-08 | 1989-01-18 | Inmunoensayo de operacion positiva. |
Country Status (8)
Country | Link |
---|---|
US (1) | US4952517A (es) |
EP (1) | EP0327843B1 (es) |
JP (1) | JP2656970B2 (es) |
AU (1) | AU611971B2 (es) |
CA (1) | CA1302876C (es) |
DE (1) | DE68908432T2 (es) |
ES (1) | ES2058344T3 (es) |
RU (1) | RU2058032C1 (es) |
Families Citing this family (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5939272A (en) * | 1989-01-10 | 1999-08-17 | Biosite Diagnostics Incorporated | Non-competitive threshold ligand-receptor assays |
US5028535A (en) * | 1989-01-10 | 1991-07-02 | Biosite Diagnostics, Inc. | Threshold ligand-receptor assay |
ES2088427T3 (es) * | 1989-03-21 | 1996-08-16 | Hygeia Sciences Ltd | Ensayo simultaneo de dos analitos. |
FR2667943B1 (fr) * | 1990-10-11 | 1994-05-13 | Jacques Toledano | Dispositif et procede pour la determination qualitative et quantitative rapide d'un ligand dans un fluide. |
CA2056698A1 (en) * | 1990-12-11 | 1992-06-12 | John L. Palmer | Assay, employing analog to digital signaling, for the presence of an analyte in a sample in conjunction with a binding component, apparatus for performing said process, and a method of performing said assay |
FI86777C (fi) * | 1990-12-31 | 1992-10-12 | Medix Biochemica Ab Oy | Foerfarande foer diagnosticering av bristningen av fosterhinnorna samt reagensfoerpackning foer anvaendning daervid |
US5468648A (en) * | 1991-05-29 | 1995-11-21 | Smithkline Diagnostics, Inc. | Interrupted-flow assay device |
US6168956B1 (en) | 1991-05-29 | 2001-01-02 | Beckman Coulter, Inc. | Multiple component chromatographic assay device |
US5869345A (en) | 1991-05-29 | 1999-02-09 | Smithkline Diagnostics, Inc. | Opposable-element assay device employing conductive barrier |
US5998220A (en) | 1991-05-29 | 1999-12-07 | Beckman Coulter, Inc. | Opposable-element assay devices, kits, and methods employing them |
US5877028A (en) | 1991-05-29 | 1999-03-02 | Smithkline Diagnostics, Inc. | Immunochromatographic assay device |
US5607863A (en) * | 1991-05-29 | 1997-03-04 | Smithkline Diagnostics, Inc. | Barrier-controlled assay device |
EP0643777A4 (en) * | 1992-01-22 | 1995-06-07 | Abbott Lab | CALIBRATION REAGENTS FOR SEMI-QUANTITATIVE BINDING ASSAYS AND DEVICES. |
US5541069A (en) * | 1992-02-28 | 1996-07-30 | Quidel Corporation | Assay having improved dose response curve |
FI94181C (fi) * | 1992-06-29 | 1995-07-25 | Medix Biochemica Ab Oy | Diagnostinen menetelmä synnytysajankohdan ennustamiseksi sekä siihen käytettävä reagenssipakkaus |
JP3479100B2 (ja) * | 1993-06-02 | 2003-12-15 | 帝国臓器製薬株式会社 | 免疫化学的簡易半定量方法および装置 |
US20060063193A1 (en) * | 1995-04-11 | 2006-03-23 | Dong-Jing Fu | Solid phase sequencing of double-stranded nucleic acids |
WO1997034150A1 (en) * | 1996-03-14 | 1997-09-18 | Abbott Laboratories | Binding members extending from particles for immunoassay |
US5879951A (en) | 1997-01-29 | 1999-03-09 | Smithkline Diagnostics, Inc. | Opposable-element assay device employing unidirectional flow |
US6046008A (en) * | 1997-04-24 | 2000-04-04 | Ia, Inc. | Immunologically based strip test utilizing ionophore membranes |
US5939252A (en) | 1997-05-09 | 1999-08-17 | Lennon; Donald J. | Detachable-element assay device |
CA2437298A1 (en) * | 2000-02-23 | 2001-08-30 | Besst-Test Aps | Method for correlating blood coagulation activity with markers in body fluids, e.g. urine |
US6673562B2 (en) | 2000-08-24 | 2004-01-06 | Spectral Diagnostics, Inc. | Differential immunoassay |
US7270959B2 (en) | 2001-07-25 | 2007-09-18 | Oakville Hong Kong Company Limited | Specimen collection container |
US7300633B2 (en) | 2001-07-25 | 2007-11-27 | Oakville Hong Kong Company Limited | Specimen collection container |
ATE465410T1 (de) | 2002-08-13 | 2010-05-15 | N Dia Inc | Vorrichtungen und verfahren zum nachweis von fruchtwasser in vaginalsekreten |
US7560272B2 (en) | 2003-01-04 | 2009-07-14 | Inverness Medical Switzerland Gmbh | Specimen collection and assay container |
US7517495B2 (en) | 2003-08-25 | 2009-04-14 | Inverness Medical Switzerland Gmbh | Biological specimen collection and analysis system |
RU2361193C2 (ru) | 2004-05-19 | 2009-07-10 | Вп Холдинг, Ллс | Оптический датчик с многослойной плазмонной структурой для усовершенствованного обнаружения химических групп посредством sers |
JP5035622B2 (ja) * | 2008-01-11 | 2012-09-26 | 積水化学工業株式会社 | 着色ラテックス |
CN107727841B (zh) | 2013-01-02 | 2021-05-25 | 凯杰科学有限责任公司 | 预测孕妇分娩时间的方法 |
US10656164B2 (en) | 2016-12-22 | 2020-05-19 | Qiagen Sciences, Llc | Screening asymptomatic pregnant woman for preterm birth |
US10935555B2 (en) | 2016-12-22 | 2021-03-02 | Qiagen Sciences, Llc | Determining candidate for induction of labor |
US11061026B2 (en) | 2017-02-17 | 2021-07-13 | MFB Fertility, Inc. | System of evaluating corpus luteum function by recurrently evaluating progesterone non-serum bodily fluids on multiple days |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4283383A (en) * | 1974-05-20 | 1981-08-11 | Technicon Instruments Corporation | Analysis of biological fluids |
NL7807532A (nl) * | 1978-07-13 | 1980-01-15 | Akzo Nv | Metaal-immunotest. |
GB2029011B (en) * | 1978-09-01 | 1983-02-02 | Coulson W | Use of a synthetic bifunctional ligand for the immunimetric determination of the concentration ratio of two solutes |
IE54109B1 (en) * | 1982-02-10 | 1989-06-21 | Boots Celltech Diagnostics | Assay |
CA1183080A (en) * | 1982-03-12 | 1985-02-26 | William F. Coulson | Use of synthetic bifunctional ligand for the immunometric determination of the concentration ratio of two solutes |
EP0114614A2 (en) * | 1983-01-25 | 1984-08-01 | Abbott Laboratories | Determination of steroid hormone glucuronides |
GB8331514D0 (en) * | 1983-11-25 | 1984-01-04 | Janssen Pharmaceutica Nv | Visualization method |
-
1988
- 1988-02-08 US US07/153,081 patent/US4952517A/en not_active Expired - Lifetime
- 1988-11-25 AU AU25983/88A patent/AU611971B2/en not_active Ceased
-
1989
- 1989-01-18 ES ES89100758T patent/ES2058344T3/es not_active Expired - Lifetime
- 1989-01-18 EP EP89100758A patent/EP0327843B1/en not_active Expired - Lifetime
- 1989-01-18 DE DE89100758T patent/DE68908432T2/de not_active Expired - Fee Related
- 1989-02-02 CA CA000589949A patent/CA1302876C/en not_active Expired - Fee Related
- 1989-02-07 RU SU894613461A patent/RU2058032C1/ru active
- 1989-02-08 JP JP1027734A patent/JP2656970B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
AU611971B2 (en) | 1991-06-27 |
JP2656970B2 (ja) | 1997-09-24 |
US4952517A (en) | 1990-08-28 |
CA1302876C (en) | 1992-06-09 |
RU2058032C1 (ru) | 1996-04-10 |
JPH01227963A (ja) | 1989-09-12 |
EP0327843A1 (en) | 1989-08-16 |
DE68908432D1 (de) | 1993-09-23 |
AU2598388A (en) | 1989-08-10 |
DE68908432T2 (de) | 1993-12-23 |
EP0327843B1 (en) | 1993-08-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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