WO2000039586A3 - Tetramethylbenzidine formulation for horseradish peroxidase enzyme assays - Google Patents

Tetramethylbenzidine formulation for horseradish peroxidase enzyme assays Download PDF

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Publication number
WO2000039586A3
WO2000039586A3 PCT/US1999/031167 US9931167W WO0039586A3 WO 2000039586 A3 WO2000039586 A3 WO 2000039586A3 US 9931167 W US9931167 W US 9931167W WO 0039586 A3 WO0039586 A3 WO 0039586A3
Authority
WO
WIPO (PCT)
Prior art keywords
formulation
tetramethylbenzidine
horseradish peroxidase
chromogen
enzyme assays
Prior art date
Application number
PCT/US1999/031167
Other languages
French (fr)
Other versions
WO2000039586A2 (en
Inventor
James E Woiszwillo
Original Assignee
Sedum Lab
James E Woiszwillo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sedum Lab, James E Woiszwillo filed Critical Sedum Lab
Priority to AU23972/00A priority Critical patent/AU2397200A/en
Publication of WO2000039586A2 publication Critical patent/WO2000039586A2/en
Publication of WO2000039586A3 publication Critical patent/WO2000039586A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/10—Benzidines
    • C12Q2326/12—3,3',5,5'-Tetramethylbenzidine, i.e. TMB

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A composition and method for detecting or measuring analyte in a sample. The composition is a tetraalkylbenzidine formulation in which a tetraalkylbenzidine chromogen is dissolved in a chromogen solvent that enhances the solubility and thereby increases the sensitivity of the chromogen when used in an assay, particularly an immunoassay. The chromogen solvent is acetronitrile. The tetraalkylbenzidine formulation is optionally diluted in an aqueous solution, such as a buffer.
PCT/US1999/031167 1998-12-30 1999-12-29 Tetramethylbenzidine formulation for horseradish peroxidase enzyme assays WO2000039586A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU23972/00A AU2397200A (en) 1998-12-30 1999-12-29 Tetramethylbenzidine formulation with increased performance for horseradish peroxidase enzyme assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11446098P 1998-12-30 1998-12-30
US60/114,460 1998-12-30

Publications (2)

Publication Number Publication Date
WO2000039586A2 WO2000039586A2 (en) 2000-07-06
WO2000039586A3 true WO2000039586A3 (en) 2000-11-09

Family

ID=22355343

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/031167 WO2000039586A2 (en) 1998-12-30 1999-12-29 Tetramethylbenzidine formulation for horseradish peroxidase enzyme assays

Country Status (2)

Country Link
AU (1) AU2397200A (en)
WO (1) WO2000039586A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107771285A (en) 2015-06-08 2018-03-06 阿奎尔诊断有限公司 Method
US11391744B2 (en) 2015-06-08 2022-07-19 Arquer Diagnostic Limited Methods and kits
CN112485244A (en) * 2020-10-14 2021-03-12 中国食品药品检定研究院 Application of 3,3',5,5' -tetramethylbenzidine in detection of benzoyl peroxide in acne-removing cosmetics

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4596770A (en) * 1984-06-01 1986-06-24 Travenol-Genetech Diagnostics Assay of peroxidase enzyme activity
EP0504663A1 (en) * 1991-03-18 1992-09-23 Bayer Corporation Peroxidase indicator system for basic media
US5374561A (en) * 1993-10-25 1994-12-20 Miles Inc. Oxidative creatinine assay

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4596770A (en) * 1984-06-01 1986-06-24 Travenol-Genetech Diagnostics Assay of peroxidase enzyme activity
EP0504663A1 (en) * 1991-03-18 1992-09-23 Bayer Corporation Peroxidase indicator system for basic media
US5374561A (en) * 1993-10-25 1994-12-20 Miles Inc. Oxidative creatinine assay

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AWANO, HIROSHI ET AL: "Absorption spectra of the cationic radical salt of 3,3',5,5'- tetramethylbenzidine in acetonitrile", BULL. CHEM. SOC. JPN. (1990), 63(7), 2101-3, XP002141291 *
SAGET, JEAN P. ET AL: "Influence of pH on the electrochemical oxidation of N,N,N',N'- tetramethylbenzidine in acetonitrile media", BULL. SOC. CHIM. FR. (1969), (4), 1395-401, XP002141290 *

Also Published As

Publication number Publication date
AU2397200A (en) 2000-07-31
WO2000039586A2 (en) 2000-07-06

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