ES2054883T3 - Procedimiento para la deteccion de acidos nucleicos. - Google Patents

Procedimiento para la deteccion de acidos nucleicos.

Info

Publication number
ES2054883T3
ES2054883T3 ES89100482T ES89100482T ES2054883T3 ES 2054883 T3 ES2054883 T3 ES 2054883T3 ES 89100482 T ES89100482 T ES 89100482T ES 89100482 T ES89100482 T ES 89100482T ES 2054883 T3 ES2054883 T3 ES 2054883T3
Authority
ES
Spain
Prior art keywords
procedure
detection
nucleic acids
hapteno
bridge
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89100482T
Other languages
English (en)
Inventor
Hans Joachim Dr Holtke
Rudolf Dr Seibl
Gudrun Dr Schmitz
Hans Robert Dr Scholer
Christop Dr Kessler
Ralf Prof Dr Mattes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2054883T3 publication Critical patent/ES2054883T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PARA LA COMPROBACION DE ACIDOS NUCLEINICOS DE SECUENCIA DEFINIDA POR HIBRIDACION CON UNA SONDA COMPLEMENTARIA DE ACIDO NUCLEINICO QUE CONTENGA UN HAPTENO ENLAZADO QUIMICAMENTE COMO MARCA, SE USA COMO HAPTENO UN ESTEROIDE QUE ESTE UNIDO AL MENOS A UNA POSICION DE LA SONDA DE ACIDO NUCLEINICO (QUE NO FORME PARTE DEL PUENTE ENLACE DE HIDROGENO) POR UN PUENTE DE AL MENOS CUATRO ATOMOS DE LARGO, Y MARCA LA SONDA HIBRIDADA POR UN ANTICUERPO DE ANTIO-HAPTENO MARCADO A SU VEZ.
ES89100482T 1988-01-12 1989-01-12 Procedimiento para la deteccion de acidos nucleicos. Expired - Lifetime ES2054883T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3800642 1988-01-12
DE3813278A DE3813278A1 (de) 1988-01-12 1988-04-20 Verfahren zum nachweis von nukleinsaeuren

Publications (1)

Publication Number Publication Date
ES2054883T3 true ES2054883T3 (es) 1994-08-16

Family

ID=25863884

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89100482T Expired - Lifetime ES2054883T3 (es) 1988-01-12 1989-01-12 Procedimiento para la deteccion de acidos nucleicos.

Country Status (7)

Country Link
US (2) US5344757A (es)
EP (1) EP0324474B1 (es)
JP (1) JPH0731194B2 (es)
DE (2) DE3813278A1 (es)
ES (1) ES2054883T3 (es)
HK (1) HK116996A (es)
WO (1) WO1989006698A1 (es)

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DE4302459A1 (de) * 1993-01-29 1994-08-04 Bayer Ag Sulfocumarinhaltige Nukleotide und deren Verwendung in Nachweisverfahren für Nukleinsäuren
US5646126A (en) * 1994-02-28 1997-07-08 Epoch Pharmaceuticals Sterol modified oligonucleotide duplexes having anticancer activity
DE4420732A1 (de) 1994-06-15 1995-12-21 Boehringer Mannheim Gmbh Vorrichtung zur Behandlung von Nukleinsäuren aus einer Probe
EP0752474A1 (en) 1995-07-07 1997-01-08 Boehringer Mannheim Gmbh Nucleic acid coding for CMP-N-acetyl-neuraminic acid hydroxylase and its use for the production of modified glycoproteins
US5989813A (en) * 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
DE69637047T2 (de) * 1995-07-13 2007-12-27 Applera Corp., Foster City Unabhängiges gerät zur extraktion, amplifikation und nachweis von nukleinsäuren
WO1997007242A1 (en) * 1995-08-16 1997-02-27 Steven Lehrer Method for detecting circulating breast cancer cells
DE19537952A1 (de) * 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
DE19608687A1 (de) * 1996-03-06 1997-09-11 Retro Tech Gmbh Verfahren und Test-Kit für den nichtradioaktiven, enzymatischen Nachweis von Reverser Transkriptase
JP3545158B2 (ja) * 1996-03-14 2004-07-21 シスメックス株式会社 遺伝子検出法
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EP0956365A4 (en) * 1996-08-28 2004-08-25 Univ Johns Hopkins Med METHOD FOR DETECTING PROLIFERATIVE CELLULAR DISORDERS
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DE19824900B4 (de) 1998-06-04 2006-05-04 Roche Diagnostics Gmbh DNA-Nachweis über einen Strangreassoziationskomplex
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EP1006184A1 (en) 1998-12-03 2000-06-07 F. Hoffmann-La Roche Ag IGF-1 receptor interacting proteins (IIPs) genes coding therefor and uses thereof
AU3274500A (en) 1999-03-18 2000-10-09 Exiqon A/S Detection of mutations in genes by specific lna primers
IL145826A0 (en) * 1999-04-08 2002-07-25 Sir Mortimer B Davis Jewish Ge Quantitative assay for expression of genes in microarray
DE19926068C1 (de) 1999-06-08 2001-01-11 Arne Skerra Muteine des Bilin-Bindungsproteins
US7169553B1 (en) 1999-08-30 2007-01-30 Roche Diagnostics, Gmbh 2-Azapurine compounds and their use
EP1099757B1 (en) 1999-11-11 2010-02-24 F. Hoffmann-La Roche Ag Process for the determination of CTp11 and for determining whether a tumor sample has metatastic potential
US6503736B1 (en) * 1999-11-12 2003-01-07 BIOMéRIEUX, INC. Antibodies to crosslinkers and methods for using the same
JP2003528625A (ja) * 2000-03-31 2003-09-30 メモレック シュトッフェル ゲゼルシャフト ミット ベシュレンクテル ハフツング メディツィニッシューモレクラー エントヴィクルンク，ケルン シグナル発生特性と、標識核酸に対する親和性を有する少なくとも１種類の基とを有する粒子の使用
JP2001288197A (ja) 2000-04-10 2001-10-16 Fuji Photo Film Co Ltd 蛍光ヌクレオチド
AU2001260309A1 (en) 2000-05-19 2001-12-03 F.Hoffmann-La Roche Ag A process for determining the tumoricidal potential of a sample by the use of a nucleic acid which is downregulated in human tumor cells
WO2002012263A1 (en) 2000-08-03 2002-02-14 Roche Diagnostics Gmbh Nucleic acid binding compounds containing pyrazolo[3,4-d]pyrimidine analogues of purin-2,6-diamine and their uses
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DE3431536A1 (de) * 1984-08-28 1986-03-13 Boehringer Mannheim Gmbh, 6800 Mannheim Derivatisierte nucleinsaeure-sequenz, verfahren zu deren herstellung sowie deren verwendung zum nachweis von nucleinsaeuren
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DE3836656A1 (de) * 1988-10-27 1990-05-03 Boehringer Mannheim Gmbh Neue digoxigenin-derivate und ihre verwendung

Also Published As

Publication number Publication date
DE3813278A1 (de) 1989-07-20
WO1989006698A1 (en) 1989-07-27
JPH0731194B2 (ja) 1995-04-10
US5702888A (en) 1997-12-30
US5344757A (en) 1994-09-06
HK116996A (en) 1996-07-12
EP0324474B1 (de) 1993-03-31
DE58903898D1 (de) 1993-05-06
EP0324474A1 (de) 1989-07-19
JPH01503647A (ja) 1989-12-07

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