KR910006496A - 바이러스 헤마 글루티닌 유전자 영역의 역전사 반응보다 선행된 폴리머레이즈 쇄반응(pcr)에 의한 인플루엔자 a바이러스의 검출방법 - Google Patents

바이러스 헤마 글루티닌 유전자 영역의 역전사 반응보다 선행된 폴리머레이즈 쇄반응(pcr)에 의한 인플루엔자 a바이러스의 검출방법 Download PDF

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KR910006496A
KR910006496A KR1019900013592A KR900013592A KR910006496A KR 910006496 A KR910006496 A KR 910006496A KR 1019900013592 A KR1019900013592 A KR 1019900013592A KR 900013592 A KR900013592 A KR 900013592A KR 910006496 A KR910006496 A KR 910006496A
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pcr
reverse transcription
virus
gene
chain reaction
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KR1019900013592A
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KR100188383B1 (ko
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아. 세루티 페테르
브레소우드 알베릭
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스타인. 부크
베링베르케 악크티엔게젤샤프트
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

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Abstract

내용 없음

Description

바이러스 헤마 글루티닌 유전자 영역의 역전사 반응보다 선행된 폴리머레이즈 쇄 반응(PCR)에 의한 인플루엔자 A바이러스의 검출방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (8)

  1. 역전사(RT) 및 후속 폴리머레이즈 쇄 반응(PCR)에 헤미글루티닌 유전자 H1의 보존 영역에서 수득된 앰플리머(amplimer)를 사용함을 특징으로 하여, 인플루엔자 A바이러스를 검출하는 방법.
  2. 제1항에 있어서, 헤마글루티닌유전자 H1 96±80 사이의 영역에서 수득된 길이가 약 20개 염기쌍(bp)인 올리고 뉴클레오타이드를 앰플리머(amplimer)를 사용하는 방법.
  3. 제2항에 있어서, 헤마글루티닌 H1 유전자의 96±20 사이의 영역에서 수득된 길이가 약 20bp의 올리고 뉴클레오타이드를 앰플리머로 사용하는 방법.
  4. 제1항에 있어서, 앰플리머(amplimer)및)를 사용하는 방법.
  5. 헤마글루티닌 H1 유전자의 보존 영역에서 수득된 앰플리머(amplimer)를 함유하는 인플루엔자 A바이러스의 검출용 시약 배합물.
  6. 제5항에 있어서, 앰플리머(amplimer)가 헤마글루티닌 H1 유전자의 96±80 및 536±80 사이의 영역에서 수득된 약 20bp를 함유하는 시약 배합물.
  7. 제5항 내지 제6항중 어느 한 항에 있어서, 앰플리머(amplimer)가 헤마글루티닌 H1 유전자의 96±20 및 536±20 사이의 영역에서 수득된 약 20bp를 함유하는 시약 배합물.
  8. 제5항에 있어서, 앰플리머(amplimer)및)를 사용하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900013592A 1989-09-02 1990-08-31 바이러스 헤마글루티닌 유전자영역의 역전사반응에 연속한 폴리머라제쇄 반응에 의한 인플루엔자 a바이러스의 검출방법 KR100188383B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE39291448 1989-09-02
DE3929144A DE3929144A1 (de) 1989-09-02 1989-09-02 Nachweis von influenza-a-virus durch polymerase-kettenreaktion (pcr) nach reverser transkription eines bereichs des viralen haemagglutinin-gens

Publications (2)

Publication Number Publication Date
KR910006496A true KR910006496A (ko) 1991-04-29
KR100188383B1 KR100188383B1 (ko) 1999-06-01

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KR1019900013592A KR100188383B1 (ko) 1989-09-02 1990-08-31 바이러스 헤마글루티닌 유전자영역의 역전사반응에 연속한 폴리머라제쇄 반응에 의한 인플루엔자 a바이러스의 검출방법

Country Status (13)

Country Link
US (1) US5187060A (ko)
EP (1) EP0416426B1 (ko)
JP (1) JPH0398600A (ko)
KR (1) KR100188383B1 (ko)
AT (1) ATE130372T1 (ko)
AU (1) AU624265B2 (ko)
CA (1) CA2024442A1 (ko)
DE (2) DE3929144A1 (ko)
DK (1) DK0416426T3 (ko)
ES (1) ES2080772T3 (ko)
GR (1) GR3018519T3 (ko)
IE (1) IE68762B1 (ko)
PT (1) PT95165B (ko)

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Also Published As

Publication number Publication date
IE903176A1 (en) 1991-03-13
JPH0398600A (ja) 1991-04-24
DE3929144A1 (de) 1991-03-07
DE59009876D1 (de) 1995-12-21
KR100188383B1 (ko) 1999-06-01
AU624265B2 (en) 1992-06-04
US5187060A (en) 1993-02-16
EP0416426A2 (de) 1991-03-13
GR3018519T3 (en) 1996-03-31
PT95165B (pt) 1997-05-28
ATE130372T1 (de) 1995-12-15
IE68762B1 (en) 1996-07-10
EP0416426A3 (en) 1991-09-11
EP0416426B1 (de) 1995-11-15
ES2080772T3 (es) 1996-02-16
PT95165A (pt) 1991-05-22
DK0416426T3 (da) 1996-03-11
CA2024442A1 (en) 1991-03-03
AU6203290A (en) 1991-03-07

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