ES2018916A6 - Un metodo de ensayo de hibridacion de acidos nucleicos. - Google Patents

Un metodo de ensayo de hibridacion de acidos nucleicos.

Info

Publication number
ES2018916A6
ES2018916A6 ES8903082A ES8903082A ES2018916A6 ES 2018916 A6 ES2018916 A6 ES 2018916A6 ES 8903082 A ES8903082 A ES 8903082A ES 8903082 A ES8903082 A ES 8903082A ES 2018916 A6 ES2018916 A6 ES 2018916A6
Authority
ES
Spain
Prior art keywords
based amplification
detection systems
amplification detection
replicative rna
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES8903082A
Other languages
English (en)
Inventor
Fei Chu Barbara Chen
Gerald Francis Joyce
Leslie Eleazer Orgel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Salk Institute for Biological Studies
Original Assignee
Salk Institute for Biological Studies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Salk Institute for Biological Studies filed Critical Salk Institute for Biological Studies
Publication of ES2018916A6 publication Critical patent/ES2018916A6/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODO DE ENAYO DE HIBRIDACION DE ACIDOS NUCLEICOS. CONSISTE EN DETECTAR ARNS AUTO REPLICABLES CAPACES DE REPLICARSE AUTOCATALITICAMENTE CON UNA POLIMERASA DE ARN DEPENDIENTE DEL ARN CORRESPONDIENTE, PRODUCIENDOSE DICHOS ARNS POR TRASNCRIPCION DE UN ADN QUE COMPRENDE UN PROMOTOR OPERATIVAMENTE ENLAZADO CON UN SEGMENTO DE ADN QUE CODIFICA DICHO ARN REPLICABLE, CUYA TRANSCRIPCION SE CATALIZA CON UNA POLIMERASA DE ARN DEPENDIENTE DE ADN QUE RECONOCE DICHO PROMOTOR DE TAL MANERA QUE SE PRODUZCAN DICHOS ARNS REPLICABLES, EN DONDE DICHA POLIMERASA DE ARN DEPENDIENTE DE ADN ESTA ASOCIADA, EN UN ADUCTO, CON UNA SECUENCIA SONDA DE OLIGONUCLEOTIDO CAPAZ DE HIBRIDARSE CON LA SECUENCIA DIANA DE ACIDO NUCLEICO, O DICHO ADN QUE CONTIENE EL PROMOTOR ESTA ASOCIADO, EN UN ADUCTO, CON UNA SECUENCIA SONDA DE OLIGONUCLEOTIDO CAPAZ DE HIBRIDARSE CON LA SECUENCIA DIANA DE ACIDO NUCLEICO. EL INVENTO ES UTIL PARA DETERMINAR LA EXISTENCIA O EL POTENCIAL DE UNA ENFERMEDAD PATOGENICA.
ES8903082A 1988-09-08 1989-09-08 Un metodo de ensayo de hibridacion de acidos nucleicos. Expired - Lifetime ES2018916A6 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24194288A 1988-09-08 1988-09-08
US24196988A 1988-09-08 1988-09-08

Publications (1)

Publication Number Publication Date
ES2018916A6 true ES2018916A6 (es) 1991-05-16

Family

ID=26934704

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8903082A Expired - Lifetime ES2018916A6 (es) 1988-09-08 1989-09-08 Un metodo de ensayo de hibridacion de acidos nucleicos.

Country Status (5)

Country Link
US (1) US5532126A (es)
EP (1) EP0433363A4 (es)
JP (1) JP2939485B2 (es)
ES (1) ES2018916A6 (es)
WO (1) WO1990002819A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5503979A (en) 1984-05-25 1996-04-02 The Trustees Of Columbia University In The City Of New York Method of using replicatable hybridzable recombinant RNA probes
US5356774A (en) * 1986-04-16 1994-10-18 The Trustees Of Columbia University In The City Of New York Replicative RNA-based amplification/detection systems
US5359100A (en) * 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
US5656731A (en) * 1987-10-15 1997-08-12 Chiron Corporation Nucleic acid-amplified immunoassay probes
CA1339991C (en) * 1988-09-08 1998-08-11 The Trustees Of Columbia University Replicative rna-based amplification/detection system
CA2001110A1 (en) * 1988-11-18 1990-05-18 Zvi G. Loewy Compositions and process for amplifying and detecting nucleic acid sequences
DE69131596T2 (de) 1990-01-10 1999-12-23 Chiron Diagnostics Corp., East Walpole Dns-abhängige rns-polymerase-transkripte als reportermoleküle zur signalverstärkung in nukleinsäure-hybridisierungsuntersuchungen
ATE174121T1 (de) * 1990-05-04 1998-12-15 Chiron Corp Protein-nukleinsäuresonden und selbige verwendende immunoassays
AU7997791A (en) * 1990-05-23 1991-12-10 Trustees Of Columbia University In The City Of New York, The Replicatable hybridizable recombinant rna probes and hybridization assay
AU738261B2 (en) * 1990-12-31 2001-09-13 Promega Corporation Nucleic acid amplification with DNA-dependent RNA polymerase activity of RNA replicases
CA2076750C (en) 1990-12-31 2003-07-08 Promega Corporation Nucleic acid amplification with dna-dependent rna polymerase activity of rna replicases
US6100024A (en) * 1991-02-08 2000-08-08 Promega Corporation Methods and compositions for nucleic acid detection by target extension and probe amplification
US6200753B1 (en) * 1993-06-03 2001-03-13 Intelligene Ltd. Detection of nucleic acid sequences
US5871914A (en) * 1993-06-03 1999-02-16 Intelligene Ltd. Method for detecting a nucleic acid involving the production of a triggering RNA and transcription amplification
CA2140081C (en) * 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US20050123926A1 (en) * 1994-01-13 2005-06-09 Enzo Diagnostics, Inc., In vitro process for producing multiple nucleic acid copies
DE4409436A1 (de) * 1994-03-19 1995-09-21 Boehringer Mannheim Gmbh Verfahren zur Bearbeitung von Nukleinsäuren
FR2723591B1 (fr) * 1994-08-10 1996-09-20 Cis Bio Int Methode d'amplification et/ou de detection d'une sequence d'acide nucleique, reactif de detection et ses applications.
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
US20050186606A1 (en) * 2004-02-11 2005-08-25 Schroeder Benjamin G. Methods and compositions for detecting nucleic acids

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4495280A (en) * 1981-05-20 1985-01-22 The Board Of Trustees Of The Leland Stanford Jr. University Cloned high signal strength promoters
US4556643A (en) * 1982-07-26 1985-12-03 Agracetus Assay method and probe for polynucleotide sequences
US4859601A (en) * 1984-03-05 1989-08-22 Smithkline Beckman Corporation Streptomyces Bgl protein gene promoter
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4647529A (en) * 1984-06-01 1987-03-03 Rodland Karin D Hybridization method of detecting nucleic acid sequences with probe containing thionucleotide
US4761367A (en) * 1984-11-07 1988-08-02 The University Of North Carolina At Chapel Hill Vectors suitable for detection of eukaryotic DNA regulatory sequences
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1339991C (en) * 1988-09-08 1998-08-11 The Trustees Of Columbia University Replicative rna-based amplification/detection system

Also Published As

Publication number Publication date
EP0433363A1 (en) 1991-06-26
US5532126A (en) 1996-07-02
WO1990002819A1 (en) 1990-03-22
EP0433363A4 (en) 1992-05-06
JP2939485B2 (ja) 1999-08-25
JPH04500457A (ja) 1992-01-30

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