ES2018917A6 - Metodo para la deteccion de secuencias diana de acido nucleico especifico. - Google Patents

Metodo para la deteccion de secuencias diana de acido nucleico especifico.

Info

Publication number
ES2018917A6
ES2018917A6 ES8903083A ES8903083A ES2018917A6 ES 2018917 A6 ES2018917 A6 ES 2018917A6 ES 8903083 A ES8903083 A ES 8903083A ES 8903083 A ES8903083 A ES 8903083A ES 2018917 A6 ES2018917 A6 ES 2018917A6
Authority
ES
Spain
Prior art keywords
based amplification
detection systems
nucleic acid
replicative rna
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES8903083A
Other languages
English (en)
Inventor
Vladimir David Axelrod
Fred Russell Kramer
Paul Modesto Lizardi
Donald Robert Mills
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Columbia University in the City of New York
Original Assignee
Columbia University in the City of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Columbia University in the City of New York filed Critical Columbia University in the City of New York
Publication of ES2018917A6 publication Critical patent/ES2018917A6/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODO PARA LA DETECCION DE SECUENCIAS DIANA DE ACIDO NUCLEICO ESPECIFICO. COMPRENDE: HIBRIDAR, EN CONDICIONES ADECUADAS, UN ADUCTO DE PROMOTOR -OLIGONUCLEOTIDO/MOLECULA DE DNA QUE COMPRENDE UNA SECUENCIA DE OLIGONUCLEOTIDO CAPAZ DE HIBRIDAR CON UNA SECUENCIA DIANA DE ACIDO NUCLEICO EN UNA MUESTRA CONTENIENDO EL MISMO; Y UNA SECUENCIA PROMOTORA OPERABLEMENTE UNIDA A UNA DNA CODIFICANTE DE RNA CAPAZ DE RECONOCER Y ENLAZARSE CON UNA RNA POLIMERASA DEPENDIENTE DE RNA A LA SECUENCIA DIANA EN LA MUESTRA; ELIMINAR EL EXCESO DE ADUCTO QUE NO SE HA HIBRIDADO; ENSAYAR EL NUMERO DE SECUENCIAS DE PROMOTOR/MOLECULA DE DNA ASOCIADAS POR HIBRIDACION CON DICHA SECUENCIA DIANA DE ACIDO NUCLEICO, UTILIZANDOLA PARA DIRIGIR LA TRANSCRIPCION DE DICHO DNA MONO- O BI-FILAMENTAR POR ASOCIACION DE DICHA SECUENCIA DE PROMOTOR/MOLECULA DNA CON UNA RNA-POLIMERASA; PERMITIR LA REPLICACION DEL PRODUCTO TRANSCRITO; Y DETECTAR LOS TRANSCRITOS REPLICADOS. APLICACION EN LA DETECCION DE SECUENCIAS DIANA DE ACIDOS NUCLEICOS.
ES8903083A 1988-09-08 1989-09-08 Metodo para la deteccion de secuencias diana de acido nucleico especifico. Expired - Lifetime ES2018917A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US24162488A 1988-09-08 1988-09-08

Publications (1)

Publication Number Publication Date
ES2018917A6 true ES2018917A6 (es) 1991-05-16

Family

ID=22911483

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8903083A Expired - Lifetime ES2018917A6 (es) 1988-09-08 1989-09-08 Metodo para la deteccion de secuencias diana de acido nucleico especifico.

Country Status (6)

Country Link
EP (1) EP0386228B1 (es)
JP (1) JP3071797B2 (es)
CA (1) CA1339991C (es)
DE (1) DE68926999T2 (es)
ES (1) ES2018917A6 (es)
WO (1) WO1990002820A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002819A1 (en) * 1988-09-08 1990-03-22 The Salk Institute For Biological Studies Replicative rna-based amplification detection systems
CA2046713A1 (en) * 1990-10-16 1992-04-17 Richard M. Martinelli Amplification of midivariant dna templates
DE69128077T2 (de) 1990-12-31 1998-02-26 Promega Corp Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen
US6100024A (en) * 1991-02-08 2000-08-08 Promega Corporation Methods and compositions for nucleic acid detection by target extension and probe amplification
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4556643A (en) * 1982-07-26 1985-12-03 Agracetus Assay method and probe for polynucleotide sequences
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
WO1990002819A1 (en) * 1988-09-08 1990-03-22 The Salk Institute For Biological Studies Replicative rna-based amplification detection systems

Also Published As

Publication number Publication date
EP0386228A4 (en) 1992-05-13
DE68926999D1 (de) 1996-09-26
JP3071797B2 (ja) 2000-07-31
EP0386228B1 (en) 1996-08-21
DE68926999T2 (de) 1997-04-03
WO1990002820A1 (en) 1990-03-22
CA1339991C (en) 1998-08-11
EP0386228A1 (en) 1990-09-12
JPH03505673A (ja) 1991-12-12

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Free format text: 2009-09-08