ES2053457T3 - Ensayo de hibridacion en fase solucion para detectar secuencias de polinucleotidos. - Google Patents

Ensayo de hibridacion en fase solucion para detectar secuencias de polinucleotidos.

Info

Publication number
ES2053457T3
ES2053457T3 ES87102576T ES87102576T ES2053457T3 ES 2053457 T3 ES2053457 T3 ES 2053457T3 ES 87102576 T ES87102576 T ES 87102576T ES 87102576 T ES87102576 T ES 87102576T ES 2053457 T3 ES2053457 T3 ES 2053457T3
Authority
ES
Spain
Prior art keywords
sample
nucleic acids
hybridization
marker
reactive point
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87102576T
Other languages
English (en)
Inventor
Nanibhushan Dattagupta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Inc filed Critical Miles Inc
Application granted granted Critical
Publication of ES2053457T3 publication Critical patent/ES2053457T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/16—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor using radioactive material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

UN PROCEDIMIENTO PARA DETERMINAR LA PRESENCIA DE UNA PARTICULAR SECUENCIA DE ACIDO NUCLEICO EN UNA MUESTRA DE ENSAYO, QUE COMPRENDE (A) MODIFICAR QUIMICAMENTE ACIDOS NUCLEICOS EN LA MUESTRA DE ENSAYO, BIEN SEA PARA INTRODUCIR UN MARCADOR O UN PUNTO REACTIVO, DE MANERA QUE CONTRIBUYA A SU POSIBILIDAD DE HIBRIDACION, (B) PONER EN CONTACTO, EN CONDICIONES DE HIBRIDACION, LOS ACIDOS NUCLEICOS DE LA MUESTRA QUIMICAMENTE MODIFICADOS, CON UNA MUESTRA DE ACIDO NUCLEICO SUSCEPTIBLE DE HIBRIDACION, LA CUAL O BIEN CUANDO LOS ACIDOS NUCLEICOS DE LA MUSTRA HAN SIDO MODIFICADOS PARA INTRODUCIR UN MARCADOR, LLEVA UN PUNTO REACTIVO, O BIEN, CUANDO LOS ACIDOS NUCLEICOS DE LA MUESTRA HAN SIDO MODIFICADOS PARA INTRODUCIR UN PUNTO REACTIVO, ESTA MARCADA, (C) PONER EN CONTACTO LA SOLUCION RESULTANTE DE LA OPERACION (B) CON UNA FORMA INMOVILIZADA DE UN ASOCIADO RECTIVO AL PUNTO REACTIVO, PARA FORMAR UNA UNION ESTABLE CON EL PUNTO REACTIVO EN LOS ACIDOS NUCLEICOS DE LA MUESTRA O EN LA MUESTRA RESPECTIVAMENTE, (D)SEPARAR LA FRACCION INMOVILIZADA RESULTANTE DE LA SOLUCION RESTANTE, Y (E) DETERMINAR LA PRESENCIA DEL MARCADOR EN LA FRACCION INMOVILIZADA QUE SE HA SEPARADO O LA DISMINUCION DEL MARCADOR EN LA SOLUCION RESTANTE.
ES87102576T 1986-03-05 1987-02-24 Ensayo de hibridacion en fase solucion para detectar secuencias de polinucleotidos. Expired - Lifetime ES2053457T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83636086A 1986-03-05 1986-03-05
US92761386A 1986-11-14 1986-11-14

Publications (1)

Publication Number Publication Date
ES2053457T3 true ES2053457T3 (es) 1994-08-01

Family

ID=27125864

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87102576T Expired - Lifetime ES2053457T3 (es) 1986-03-05 1987-02-24 Ensayo de hibridacion en fase solucion para detectar secuencias de polinucleotidos.

Country Status (12)

Country Link
EP (1) EP0237833B1 (es)
JP (1) JP2613203B2 (es)
KR (1) KR870009031A (es)
AU (1) AU6972487A (es)
CA (1) CA1290664C (es)
DE (1) DE3783485T2 (es)
DK (1) DK112187A (es)
ES (1) ES2053457T3 (es)
FI (1) FI870922A (es)
GR (1) GR3006858T3 (es)
IL (1) IL81729A0 (es)
NO (1) NO870612L (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO870613L (no) * 1986-03-05 1987-09-07 Molecular Diagnostics Inc Deteksjon av mikroorganismer i en prŸve inneholdende nukleinsyre.
AU601021B2 (en) * 1987-03-11 1990-08-30 Molecular Diagnostics, Inc. Assay for necleic acid sequences in a sample
FI80476C (fi) * 1987-10-09 1990-06-11 Orion Yhtymae Oy Foerbaettrat hybridisationsfoerfarande, vid foerfarandet anvaent redskap och reagensfoerpackning.
US6054266A (en) * 1987-12-21 2000-04-25 Applied Biosystems, Inc. Nucleic acid detection with separation
DE3804243A1 (de) * 1988-02-11 1989-08-24 Max Planck Gesellschaft Teils einzel- und teils doppelstraengige nukleinsaeuresonde und verfahren zu ihrer herstellung
EP0406280A1 (en) * 1988-03-25 1991-01-09 Akzo Nobel N.V. Method for amplifying and detecting nucleic acid in a test liquid
US5856092A (en) * 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
ES2059895T3 (es) * 1989-06-23 1994-11-16 Canon Kk Metodo para la deteccion de acido nucleico.
DE3940597A1 (de) * 1989-12-08 1991-06-13 Bayer Ag Verfahren zur herstellung von di- und trialkyl-4'-phthalimidomethyl-furocumarinen
US5776672A (en) * 1990-09-28 1998-07-07 Kabushiki Kaisha Toshiba Gene detection method
US6037127A (en) * 1994-03-31 2000-03-14 E. I. Du Pont De Nemours And Company Method for detection of non-denatured nucleic acid fragments
GB9408607D0 (en) * 1994-04-29 1994-06-22 Dynal As Assay
DE19516196A1 (de) * 1995-05-08 1996-11-14 Boehringer Mannheim Gmbh Verfahren zum quantitativen Nachweis von Nukleinsäuren
JP4580875B2 (ja) 2006-01-20 2010-11-17 株式会社東芝 核酸検出法のためのプライマーの設計方法
US8093063B2 (en) * 2007-11-29 2012-01-10 Quest Diagnostics Investments Incorporated Assay for detecting genetic abnormalities in genomic nucleic acids
DE102010000906B4 (de) * 2009-01-22 2012-07-12 RUHR-UNIVERSITäT BOCHUM Konjugate für den Nachweis doppelsträngiger Nukleinsäuren

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1222680A (en) * 1983-07-05 1987-06-09 Nanibhushan Dattagupta Testing dna samples for particular nucleotide sequences
JPS6066998A (ja) * 1983-09-19 1985-04-17 Fuji Photo Film Co Ltd オ−トラジオグラフィ−による遺伝子のスクリ−ニング方法
US4599303A (en) * 1983-12-12 1986-07-08 Hri Associates, Inc. Nucleic acid hybridization assay employing probes crosslinkable to target sequences
IL73577A (en) * 1983-12-12 1989-10-31 Miles Lab Method and reagent system for detecting dna or rna sequences in a test medium containing single stranded dna or rna using antibodies to intercalation complexes with double stranded nucleic acid
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
NO870613L (no) * 1986-03-05 1987-09-07 Molecular Diagnostics Inc Deteksjon av mikroorganismer i en prŸve inneholdende nukleinsyre.

Also Published As

Publication number Publication date
DE3783485D1 (de) 1993-02-25
JPS62282599A (ja) 1987-12-08
KR870009031A (ko) 1987-10-22
GR3006858T3 (es) 1993-06-30
NO870612L (no) 1987-09-07
DK112187D0 (da) 1987-03-04
JP2613203B2 (ja) 1997-05-21
EP0237833A3 (en) 1991-01-16
DE3783485T2 (de) 1993-05-06
AU6972487A (en) 1987-09-10
CA1290664C (en) 1991-10-15
IL81729A0 (en) 1987-10-20
DK112187A (da) 1987-09-06
FI870922A (fi) 1987-09-06
NO870612D0 (no) 1987-02-17
EP0237833B1 (en) 1993-01-13
EP0237833A2 (en) 1987-09-23
FI870922A0 (fi) 1987-03-03

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