ES2038572T1 - Un detector de dispersion de luz por hplc para biopolimeros. - Google Patents

Un detector de dispersion de luz por hplc para biopolimeros.

Info

Publication number
ES2038572T1
ES2038572T1 ES199192900681T ES92900681T ES2038572T1 ES 2038572 T1 ES2038572 T1 ES 2038572T1 ES 199192900681 T ES199192900681 T ES 199192900681T ES 92900681 T ES92900681 T ES 92900681T ES 2038572 T1 ES2038572 T1 ES 2038572T1
Authority
ES
Spain
Prior art keywords
detector
light
scattered
scattered light
data
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
ES199192900681T
Other languages
English (en)
Inventor
Gavin D. Dollinger
Robert L. Cunico
Michael G. Kunitani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Publication of ES2038572T1 publication Critical patent/ES2038572T1/es
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N2021/4704—Angular selective
    • G01N2021/4726—Detecting scatter at 90°
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
    • G01N21/53—Scattering, i.e. diffuse reflection within a body or fluid within a flowing fluid, e.g. smoke
    • G01N21/532—Scattering, i.e. diffuse reflection within a body or fluid within a flowing fluid, e.g. smoke with measurement of scattering and transmission
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Materials For Medical Uses (AREA)

Abstract

UN DETECTOR FOTODISPERSOR DE HPLC PARA BIOPOLIMEROS. UN FOTODISPERSOR DE GRAN ANGULO QUE UTILIZA LA DISPERSION RAYLEIGH CLASICA. UNA FUENTE LUMINICA DE ARCO DE GRAN INTENSIDAD, FILTRADA PARA QUE SOLO UNA LONGITUD DE ONDA ILUMINE UNA CELULA DE FLUJO. A TRAVES DE LA CELULA DE FLUJO, FLUYEN PARTICULAS MUY PEQUEÑAS TALES COMO PROTEINAS BIOLOGICAS EN DISOLUCION TRAS LA SEPARACION POR HPLC O ALGUNOS MEDIOS MAS. UN DETECTOR DE UV GENERA DATOS RESPECTO A LA CONCENTRACION DE PESO DE LAS PARTICULAS DE ELUCION Y UN FOTODETECTOR QUE RECOGE LA LUZ DISPERSA EN ANGULOS DE 90 (GRADOS) APROXIMADAMENTE GENERA UNA SEÑAL DE LUZ DISPERSA. TAMBIEN SE MIDE LA INTENSIDAD DE LA LUZ INCIDENTE. A CONTINUACION, SE CALCULA LA MEDIA DEL PESO MOLECULAR UTILIZANDO LOS DATOS SOBRE LA LUZ DISPERSA E INCIDENTE, LOS DATOS DE CONCENTRACION LUMINICA Y UNA RELACION MATEMATICA SIMPLIFICADA DE LA QUE SE HAN ELIMINADO EL FACTOR P DE TAMAÑO Y LOS COEFICIENTES VIRALES.
ES199192900681T 1990-10-23 1991-10-23 Un detector de dispersion de luz por hplc para biopolimeros. Pending ES2038572T1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/601,840 US5269937A (en) 1990-10-23 1990-10-23 HPLC light scattering detector for biopolymers

Publications (1)

Publication Number Publication Date
ES2038572T1 true ES2038572T1 (es) 1993-08-01

Family

ID=24408976

Family Applications (1)

Application Number Title Priority Date Filing Date
ES199192900681T Pending ES2038572T1 (es) 1990-10-23 1991-10-23 Un detector de dispersion de luz por hplc para biopolimeros.

Country Status (9)

Country Link
US (1) US5269937A (es)
EP (1) EP0505564B1 (es)
JP (1) JP3253614B2 (es)
AT (1) ATE147161T1 (es)
CA (1) CA2072004C (es)
DE (2) DE69123957T2 (es)
ES (1) ES2038572T1 (es)
GR (1) GR930300025T1 (es)
WO (1) WO1992007244A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113916730A (zh) * 2021-12-13 2022-01-11 济南微纳颗粒仪器股份有限公司 一种纳米级粒度检测装置

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5844685A (en) * 1996-07-30 1998-12-01 Bayer Corporation Reference laser beam sampling apparatus
FR2773880B1 (fr) * 1998-01-19 2000-03-17 Cilas Dispositif pour determiner la repartition granulometrique d'un melange de particules
US6316185B1 (en) * 1999-09-29 2001-11-13 Mountain View Pharmaceuticals, Inc. Quantitation of viruses by light scattering
EP1102059B1 (en) * 1999-11-18 2005-10-05 Matsushita Electric Industrial Co., Ltd. Method and apparatus for measuring a scattered light
GB2383127B (en) * 2001-12-12 2004-10-20 Proimmune Ltd Device and method for investigating analytes in liquid suspension or solution
GB2390893A (en) * 2002-07-15 2004-01-21 Pcme Ltd Method and apparatus for monitoring particles in a stack
WO2005019419A2 (en) * 2003-07-31 2005-03-03 Singulex, Inc. Co-detection of single polypeptide and polynucleotide molecules
US20080021674A1 (en) * 2003-09-30 2008-01-24 Robert Puskas Methods for Enhancing the Analysis of Particle Detection
US8049888B2 (en) * 2004-03-01 2011-11-01 Firma Cytecs Gmbh Device for measuring light emitted by microscopically small particles or biological cells
WO2006036182A2 (en) * 2004-09-28 2006-04-06 Singulex, Inc. System and method for spectroscopic analysis of single particles
US9040305B2 (en) * 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties
GB2422897A (en) 2005-02-02 2006-08-09 Pcme Ltd A monitor for monitoring particles flowing in a stack
US7630076B2 (en) * 2005-07-26 2009-12-08 University Of Connecticut Dual-detector systems and methods having utility in biomolecular measurements
US7923238B2 (en) * 2006-02-10 2011-04-12 Bio-Rad Laboratories, Inc. Multi-channel electroporation system
CA3086149A1 (en) 2006-04-04 2007-10-11 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
US7838250B1 (en) 2006-04-04 2010-11-23 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
US20070291265A1 (en) * 2006-04-13 2007-12-20 Mississippi State University Optical apparatus for simultaneously measuring the scattering and concentration of signals of macromolecules in a flow cell
US7576549B2 (en) * 2006-09-21 2009-08-18 Bio-Rad Laboratories, Inc. Methods for measuring sample resistance in electroporation
US7750605B2 (en) 2006-09-21 2010-07-06 Bio-Rad Laboratories, Inc. Controlling an electrical signal sent to a sample load using a pulse modulated resistance
US7593101B2 (en) * 2007-04-10 2009-09-22 Schlumberger Technology Corporation High-pressure cross-polar microscopy cells having adjustable fluid passage and methods of use
WO2009025898A2 (en) * 2007-05-24 2009-02-26 The Research Foundation Of The State University Of New York Dual detector capillary waveguide biosensor and method for use thereof
WO2009012255A2 (en) * 2007-07-17 2009-01-22 Hach Company Spatial frequency optical measurement instrument and method
CN103543094B (zh) 2007-12-19 2017-06-09 神谷来克斯公司 单分子检测用扫描分析器和使用方法
US20090234202A1 (en) * 2008-03-05 2009-09-17 Goix Philippe J Method and compositions for highly sensitive detection of molecules
GB2464183A (en) * 2008-09-19 2010-04-14 Singulex Inc Sandwich assay
WO2010144358A1 (en) 2009-06-08 2010-12-16 Singulex, Inc. Highly sensitive biomarker panels
US8195405B2 (en) * 2009-06-30 2012-06-05 Wyatt Technology Corporation Method for characterizing reversible association of macromolecules at high concentration
CA2798149A1 (en) 2010-05-06 2011-11-10 Singulex, Inc Methods for diagnosing, staging, predicting risk for developing and identifying treatment responders for rheumatoid arthritis
US9001319B2 (en) 2012-05-04 2015-04-07 Ecolab Usa Inc. Self-cleaning optical sensor
US10132736B2 (en) 2012-05-24 2018-11-20 Abbvie Inc. Methods for inspection of protein particles in a liquid beneficial agent
JP5891958B2 (ja) * 2012-06-01 2016-03-23 株式会社デンソー 液体成分センサ
EP2937681B1 (en) * 2012-12-21 2019-12-18 Sony Corporation Particle detection device and particle detection method
GB201304631D0 (en) * 2013-03-14 2013-05-01 Malvern Instr Ltd Monomer detection in protein separation
JP6191477B2 (ja) * 2014-01-24 2017-09-06 株式会社島津製作所 粒子径測定装置及び粒子径測定方法
DE102014007355B3 (de) * 2014-05-19 2015-08-20 Particle Metrix Gmbh Verfahren der Partikel Tracking Aalyse mit Hilfe von Streulicht (PTA) und eine Vorrichtung zur Erfassung und Charakterisierung von Partikeln in Flüssigkeiten aller Art in der Größenordnung von Nanometern
US20150355147A1 (en) * 2014-06-06 2015-12-10 Biogénesis Bagó Uruguay S.A. High throughput quantification and characterization of foot and mouth disease virus and products thereof
US9470625B1 (en) * 2015-05-28 2016-10-18 National Chung Cheng University Method of examining purity of dendrimers
US11119079B2 (en) * 2017-08-17 2021-09-14 Daylight Solutions, Inc. Liquid chromatography analyzer system with on-line analysis of eluting fractions
CN111448448A (zh) * 2017-12-11 2020-07-24 马尔文帕纳科 通过尺寸排阻色谱法确定颗粒尺寸分布
WO2020090581A1 (ja) * 2018-10-30 2020-05-07 アルプスアルパイン株式会社 流路プレート、分析装置及び分析方法
US11965900B2 (en) * 2018-11-09 2024-04-23 Wyatt Technology, Llc Indicating a status of an analytical instrument on a screen of the analytical instrument

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2794502A (en) * 1956-02-14 1957-06-04 Andrew J Toti Vertical slat blind
JPS456225Y1 (es) * 1965-01-30 1970-03-27
JPS54109494A (en) * 1978-02-16 1979-08-28 Teikoku Hormone Mfg Co Ltd Method of measuring antigennantibody reaction
DE2918342A1 (de) * 1979-05-07 1980-11-20 Behringwerke Ag Latex-reagenz
EP0088760A1 (en) * 1981-04-13 1983-09-21 AUSTRALIAN NUCLEAR SCIENCE & TECHNOLOGY ORGANISATION Measuring the concentration of gaseous hydrogen fluoride
NO156917C (no) * 1985-07-16 1987-12-16 Harald B Steen Anordning for maaling av biologiske cellers lysspredning i vaeskestroemsfotometere.
US4796995A (en) * 1985-12-23 1989-01-10 The United States Department Of Energy Optically active biological particle distinguishing apparatus
US4732202A (en) * 1986-04-18 1988-03-22 Hunter Douglas Inc. Vertical louvre blind traveller
GB8621426D0 (en) * 1986-09-05 1986-10-15 Health Lab Service Board Particle analysis
SE463118B (sv) * 1988-02-26 1990-10-08 Btg Kaelle Inventing Ab Foerfarande och anordning foer bestaemning av koncentrationen av ett aemne som aer bundet till partiklar i ett stroemmande medium
US4934811A (en) * 1988-04-01 1990-06-19 Syntex (U.S.A.) Inc. Apparatus and method for detection of fluorescence or light scatter

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113916730A (zh) * 2021-12-13 2022-01-11 济南微纳颗粒仪器股份有限公司 一种纳米级粒度检测装置
CN113916730B (zh) * 2021-12-13 2022-10-28 济南微纳颗粒仪器股份有限公司 一种纳米级粒度检测装置

Also Published As

Publication number Publication date
JPH05505882A (ja) 1993-08-26
JP3253614B2 (ja) 2002-02-04
ATE147161T1 (de) 1997-01-15
EP0505564B1 (en) 1997-01-02
GR930300025T1 (en) 1993-05-24
EP0505564A1 (en) 1992-09-30
US5269937A (en) 1993-12-14
DE69123957D1 (de) 1997-02-13
WO1992007244A1 (en) 1992-04-30
CA2072004C (en) 2000-04-04
DE505564T1 (de) 1993-05-19
CA2072004A1 (en) 1992-04-24
DE69123957T2 (de) 1997-04-30

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