EP3626343A1 - Dispositif de retenue destiné à retenir un récipient de pipetage d'un dispositif de pipetage - Google Patents

Dispositif de retenue destiné à retenir un récipient de pipetage d'un dispositif de pipetage Download PDF

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Publication number
EP3626343A1
EP3626343A1 EP18195162.5A EP18195162A EP3626343A1 EP 3626343 A1 EP3626343 A1 EP 3626343A1 EP 18195162 A EP18195162 A EP 18195162A EP 3626343 A1 EP3626343 A1 EP 3626343A1
Authority
EP
European Patent Office
Prior art keywords
pipetting
air chamber
holding
holding device
container
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP18195162.5A
Other languages
German (de)
English (en)
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EP3626343B1 (fr
Inventor
Tobias David
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to FIEP18195162.5T priority Critical patent/FI3626343T3/en
Priority to EP18195162.5A priority patent/EP3626343B1/fr
Priority to PL18195162.5T priority patent/PL3626343T3/pl
Priority to CN201910813541.9A priority patent/CN110898869B/zh
Priority to US16/575,304 priority patent/US11633731B2/en
Publication of EP3626343A1 publication Critical patent/EP3626343A1/fr
Application granted granted Critical
Publication of EP3626343B1 publication Critical patent/EP3626343B1/fr
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Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0213—Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/54—Supports specially adapted for pipettes and burettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0672—Swellable plugs

Definitions

  • the invention relates to a holding device for holding a pipetting container, in particular a serological pipette, on a pipetting device.
  • Such pipetting devices are commonly used in medical, biological, biochemical, chemical and other laboratories. They are used in the laboratory for the transport and transfer of fluid samples, in particular for precise dosing of the samples.
  • pipetting devices e.g. liquid samples by means of negative pressure in pipetting containers, e.g. Serological pipettes or pipette tips, sucked in, held there, and released again at the destination.
  • the pipetting devices include e.g. hand-held pipetting devices or automatically controlled pipetting devices, in particular computer-controlled pipetting devices. They are usually air cushion pipetting devices. An air cushion is provided in the latter, the pressure of which is reduced when the sample is taken up into the pipetting container, as a result of which the sample is sucked into the pipetting container by means of negative pressure.
  • Pipetting devices are usually designed to dispense fluid samples with volumes in the range of e.g. Pipette 0.1 ml to 100 ml.
  • Pipetting devices are manually or electrically operated devices.
  • the term “pipetting” here encompasses both the sampling by suction by means of negative pressure and the sample dispensing by gravitation and / or pressing out by means of excess pressure.
  • Pipetting devices that are used for pipetting with serological pipettes are also referred to as pipetting aids.
  • a suction / pressure line is usually used for pipetting, the activity of which can be controlled by the operator using suitable valves in the housing body.
  • Pipetting aids are used to handle liquid samples with maximum volumes typically between 1 ml and 100 ml. Depending on the maximum liquid volume required different serological pipettes are used with the pipetting aid.
  • a serological pipette generally has a cylindrical container which is elongated along a longitudinal axis and has a liquid exchange opening at the lower end and a tubular end section which is open at the top.
  • the side wall of the container can be provided with a scale which characterizes the metering volumes; this is then also referred to as a measuring pipette.
  • the liquid is sucked into the serological pipette via a negative pressure in the interior of the serological pipette.
  • the interior of the serological pipette is fluidly connected to the pipetting channel of an electrically operated pumping device of the pipetting aid in a pipetting position, also referred to as a plug-on position, in which the serological pipette is connected to the connecting section of the pipetting aid, as a rule by being plugged on.
  • the electrically driven pump device is usually a diaphragm pump which is suitable for pipetting and which can therefore generate both a negative pressure and an excess pressure.
  • the interior Via the pipetting channel, the interior can be subjected to a controllable underpressure or overpressure, or it can be ventilated, that is, adjusted to the ambient pressure.
  • a negative pressure generated in the interior of the serological pipette is present in the serological pipette in the air cushion in the interior above the sucked-in liquid sample.
  • a pipette tip is usually a disposable item.
  • a pipette tip generally has a conical container which is elongated along a longitudinal axis and has a liquid exchange opening at the lower end and a conical and tubular end section which is open at the upper end. The liquid is sucked into the pipette tip via a negative pressure in the interior of the pipette tip.
  • the interior of the pipette tip is in a pipetting position, also referred to as a plug-on position, in which the pipette tip - usually by being plugged on - with the connecting section of the piston-stroke pipette is fluidly connected to the pipetting channel of the piston-type pipette, which is acted upon by the vacuum / positive pressure via a mechanically and / or electrically movable cylinder / cylinder piston.
  • a mechanically movable cylinder piston the movement is generated manually by a user; in the case of the electrically movable cylinder piston, an electrical energy source is provided for the drive.
  • An example of a commercially available, hand-held, electrical pipetting aid is the Eppendorf Easypet® 3 from Eppendorf AG, Hamburg, Germany.
  • An example of a commercially available, hand-held, electric piston stroke pipette is the Eppendorf Xplorer® plus from Eppendorf AG, Hamburg, Germany.
  • An example of a commercially available, hand-held, mechanical piston stroke pipette is the Eppendorf Research® plus from Eppendorf AG, Hamburg, Germany.
  • a connection is a clamp connection, which is established by a user or an automatic pipetting device placing the pipetting container on the connecting section.
  • the nature of the clamp connection established in this way therefore depends on the push-on process. Since the insertion process carried out by the user depends on the individual user behavior, the correct formation of the necessary connection between the pipetting container and the connecting section depends on the user, which represents a possible source of error.
  • a holding device for holding a pipetting container on a pipetting device in which a connecting section is provided with an inflatable air chamber, which in the inflated state establishes a clamping connection between the connecting section and the tubular end of the pipetting container.
  • a disadvantage of this is that this arrangement is prone to air chamber leakage, which jeopardizes the reliability of the connection.
  • the holding device according to the invention has the advantage that the clamp connection is largely independent of the plugging process with which a user or an automatic machine attaches the pipetting container to the connecting section. Establishing and releasing the clamp connection is a separate process that is controlled independently of the user or independently of the plug-on process. This makes the connection between the pipetting container and the pipetting device more reliable.
  • the connection between the pipetting container and the pipetting device produced by the holding device according to the invention is independent of a leak or malfunction of the air chamber device, since the clamping force is provided by the spring device, which works independently of the air chamber device. This also makes the connection between the pipetting container and the pipetting device more reliable.
  • the clamping device is also set up to form an airtight seal between the connecting section and the tubular end of the pipetting container in the pipetting position. This eliminates the need for additional sealants to enable leak-free pipetting in the pipetting position.
  • the holding device may comprise a sealing device, for example a tubular or annular seal may have a hollow truncated cone, in particular the shape, whereby the seal in its interior in can taper in the axial direction in order to reliably seal pipe sections of different diameters, as is the case in practice with different pipetting containers.
  • a sealing device for example a tubular or annular seal may have a hollow truncated cone, in particular the shape, whereby the seal in its interior in can taper in the axial direction in order to reliably seal pipe sections of different diameters, as is the case in practice with different pipetting containers.
  • the connecting section is tubular and is suitable for being connected to the tubular end of a pipetting container, in particular a serological pipette.
  • the connecting section preferably extends along a virtual longitudinal axis A, so that it is suitable for being connected to a, in particular straight, tubular end of a pipetting container arranged coaxially along the axis A.
  • the connecting section is set up to receive the tubular end of the pipetting container in its interior in the pipetting position.
  • the connecting section has a receiving space which is open to the outside and is suitable for receiving the tubular end of a pipetting container.
  • the receiving space has an opening and an inner region, the diameter of which is larger than the outer diameter of the tubular end of a pipetting container.
  • the clamping device is set up to hold the tubular end arranged in the receiving position in the receiving space by means of the spring device by establishing the clamping connection or the pipetting position on the connecting section.
  • the connecting section is set up to engage in the tubular end of the pipetting container in the pipetting position.
  • the connecting section has an outer diameter which is smaller than the inner diameter of the tubular end of a pipetting container.
  • the connecting section also has a pipetting channel which is connected in the pipetting position to the interior of the pipetting container and which can be or is connected to the pipetting channel of a pipetting device.
  • a negative pressure applied to the pipetting channel in the pipetting position then causes a fluid sample to be drawn into the pipetting container when its fluid exchange opening, which can be arranged at the end of the pipetting container, is immersed in the fluid sample.
  • a pressure in the pipetting channel or a ventilated state of the pipetting channel which can be adjustable, for example by temporarily connecting the inside of the pipetting channel to the outside atmosphere, causes a fluid sample contained in the pipetting container to be released from the fluid exchange opening or to leak.
  • the pipetting channel is connected to the receiving space, which can mean that a pipetting channel, in particular a tubular line, of the holding device or a pipetting device is connected or shaped to the receiving space in order to be integrally connected to the receiving space go.
  • the pipetting channel is arranged within the connecting section and ends in the pipetting position in the interior of the pipetting container.
  • the clamping device is arranged on the connecting section and has a spring device for producing the clamping connection and an air chamber device for releasing the clamping connection. This decoupling of the functions for generating the clamp connection and releasing this clamp connection achieves particular reliability.
  • the clamping device is preferably integrated within a base body of the connecting section.
  • the clamping device can also be arranged outside a base body of the connecting section.
  • the clamping device can have one or more fixing elements which are clamped in the pipetting position between the spring device and the tubular end of the pipetting container and which fix it in particular by a frictional force in the pipetting position.
  • the at least one fixing element can be by a Elastomer molded part are formed that in particular also forms the air chamber or at least one wall of the air chamber.
  • the spring device is set up to exert a spring force by means of which the tubular end of the pipetting container is held on the connecting section. This position of the clamping device is referred to as the pipetting position.
  • the air chamber device is set up to be deformed, in particular starting from the pipetting position, by a negative pressure applied in the air chamber device, as a result of which the spring device is elastically deformed, so that an existing clamping connection is released or the tubular end of a pipetting container can be accepted.
  • This open position of the clamping device is also referred to as the acceptance position.
  • the spring device has in particular at least one, in particular exactly one spring element.
  • This is preferably non-metallic and preferably consists of a polymeric material, in particular an elastomeric material.
  • the spring element consisting of a polymeric or elastomeric material preferably has one, several or a plurality of cavities in order to allow a pressure change acting on the spring element from outside to act on the interior of the elastomeric material.
  • the polymeric or elastomeric material is preferably a plastic foam, in particular a polyurethane foam or silicone.
  • the plastic foam is preferably open-pore in order to allow the external pressure change to also act inside the plastic foam.
  • the spring element is preferably shaped to be arranged in an air chamber of the air chamber arrangement.
  • the spring element is tensioned or compressed when a vacuum is applied in the air chamber.
  • At least one spring element or precisely one spring element is preferably provided, which is shaped in the form of a ring or a tube.
  • the spring element can be arranged coaxially to the tubular end of the pipetting container in order to exert a uniformly radially acting spring force.
  • the spring element can be connected to the connecting section by the at least one air chamber device.
  • the spring element be connected to the connecting section, in particular by a positive and / or non-positive and / or material connection.
  • the spring device preferably has a spring element which effects the spring force and which is arranged in an air chamber of the air chamber device.
  • the spring device preferably has an elastomeric foam part acting as a spring element or consists of this foam part.
  • the foam part is preferably tubular and the air chamber is preferably designed to receive the foam part.
  • the foam part preferably consists of an open-pore plastic foam, in particular a polyurethane foam, or has such a material.
  • the spring element is preferably tensioned in the pipetting position and in particular compressed.
  • the spring element is preferably less tensioned and compressed in the pipetting position in which the pipetting container is plugged onto the connecting section than in the accepting position of the clamping device, in which no pipetting container is yet connected to the connecting section. While a compressed spring element is preferred, it is also possible for the spring element to be set up to be tensioned by an expansion.
  • a multi-element spring element or more than one spring element can also be provided, which is arranged in particular coaxially with the connecting section.
  • the clamping device is preferably arranged in a rest position which corresponds to the pipetting position. In the rest position, no pipetting container is connected to the connecting section, the spring device is maximally relaxed or completely relaxed.
  • the air chamber device is set up to be deformed in the pipetting position by a negative pressure applied in the air chamber device, as a result of which the spring device is elastically deformed, so that the clamping connection is released.
  • the air chamber device preferably has an elastically deformable membrane element .
  • the membrane element preferably forms part of an air chamber of the air chamber device or forms the air chamber.
  • the membrane element is preferably formed by an elastomeric material or has such a material, in particular a silicone. In addition to silicone, a fluoroelastomer (eg FKM) can also be used.
  • the elastomeric material is preferably a thermoplastic elastomer.
  • the membrane element is preferably formed by an elastomer molded part.
  • the membrane element is preferably essentially tubular, in particular tubular, and is preferably arranged coaxially with respect to a longitudinal axis A of the connecting section.
  • the membrane element is preferably detachable or non-detachable - insoluble means in particular "non-destructively detachable" - connected to the connecting section.
  • the membrane element is preferably arranged on the connecting section in order to form the at least one air chamber of the air chamber device with an inner wall or outer wall of the connecting section.
  • the air chamber can additionally be formed by one or more sealing elements, which can in particular be arranged between the connecting section and the membrane element.
  • the membrane element and / or the elastomer molded part forming it can also have two substantially tubular, in particular tubular, membrane walls, which are preferably arranged coaxially with respect to a longitudinal axis A of the connecting section. These membrane walls can have outer walls of an air chamber of the air chamber device.
  • the spring device can in particular be arranged between these membrane walls.
  • the air chamber is preferably tubular and in particular arranged coaxially with respect to a longitudinal axis A of the connecting section.
  • the air chamber device preferably has a connection by means of which the negative pressure can be applied in the air chamber device.
  • the clamping device is set up in such a way that a deflection movement of a membrane element and / or fixing element can be generated by the negative pressure, this deflection movement tensioning the spring device due to its elastic deformation.
  • the invention also relates to a pipetting device for pipetting a fluid sample by suction into a pipetting container by means of an air under a pipetting pressure, which has exactly one or more holding devices according to the invention.
  • the latter is in particular a multi-channel pipetting device, by means of which a plurality of fluid samples can be pipetted simultaneously from a multiple sample container, in particular a microtiter plate.
  • a plurality of holding devices are preferably arranged next to one another and preferably connected to the same pipetting channel.
  • the pipetting device preferably has a pressure change device, in particular a pump device for generating the pipetting pressure in a pipetting channel, which extends through the pipetting device into the connecting section of the holding device, the air chamber device of the holding device being connected to a suction channel in the pipetting device in which the in the vacuum chamber acting vacuum can be applied.
  • the pipetting device preferably has a valve device by means of which either a fluid connection of the suction inlet of the pressure change device, in particular pump device, to the pipetting channel or a fluid connection of the suction inlet of the pressure change device, in particular pump device, is established with the suction channel.
  • the pump device comprises at least one pump, preferably exactly one pump, in particular one Diaphragm pump.
  • the valve device preferably has at least one 3/2-way valve, preferably two 3/2-way valves.
  • the pipetting device preferably has a first operating element with which the user controls the pipetting performance of the suction of the fluid sample and thus the speed of the sample pick-up.
  • the pipetting device preferably has a second operating element with which the user controls the pipetting performance of the dispensing of the fluid sample and thus the speed of the dispensing of the sample.
  • the control device is preferably set up and / or a first 3/2-way valve interacts with the pressure changing device, the pipetting channel and the suction channel in such a way that when the first operating element is actuated, the suction channel is closed and the pipetting channel with the pressure changing device-eg pump device- , in particular the input of the pressure change device, is connected to the fact that a negative pressure is generated in the pipetting channel with which the fluid sample can be sucked into the pipetting container.
  • the pressure changing device-eg pump device- in particular the input of the pressure change device
  • the control device is preferably set up and / or a second 3/2-way valve interacts with the pressure change device, the pipetting channel and the suction channel in such a way that the suction channel is closed when the second control element is actuated and with the pressure change device, for example pump device, in particular the output of the pressure change device is connected to the fact that an atmospheric pressure or overpressure is generated in the pipetting channel with which the fluid sample can be dispensed from the pipetting container.
  • the pipetting device preferably has an operating device by means of which the user can control the clamping device.
  • an additional control element switch, switch etc.
  • switches the valves in particular and switches on the pressure change device, in particular the pump device in particular the pump device.
  • a three-position button is preferably used, by means of which the three states of the suction channel “vacuum”, "off", "pressure” can be set in particular.
  • the control unit can be used to build up a vacuum, in particular time-controlled, during the first actuation. The second time the control panel is pressed, preferably causes the vacuum reduction and thereby the pipetting position is established by means of the spring element.
  • the pipetting device preferably has an electronic control device, at least one control element for activating and / or deactivating the pipetting of the fluid sample by pipetting into the pipetting container and in particular at least one control element for releasing the clamping connection of the pipetting container to the holding device, the control device preferably being used to control the Pump device and the valve device is set up.
  • the control device is preferably set up so that the fluid connection between the pressure change device, in particular the pump device, and the pipetting channel is closed when the fluid connection between the pump device and the suction channel is open and in particular the pump device is activated.
  • the control device is preferably set up so that the fluid connection between the pressure change device, in particular the pump device, and the suction channel is closed when the fluid connection between the pump device and the pipetting channel is open and in particular the pump device is activated.
  • the intake duct can be designed such that it can be ventilated by providing a connecting duct to the ambient atmosphere which is controlled or opened by the control device. Ventilation enables the negative pressure in the air chamber device to be reduced and the spring element to develop its clamping force.
  • the pipetting device can be set up such that the holding device is integrated as a fixed component in the pipetting device.
  • the holding device can therefore be set up as an integral part of a pipetting device.
  • the pipetting channel of the pipetting device is preferably fluidly connected to a pipetting channel of the holding device and / or a receiving space of the holding device.
  • the operating part for operating the clamping device is a component of the pipetting device, in particular — can be operated by a user — integrated into the housing of the pipetting device.
  • the pipetting device has a pressure change device, in particular a pump device for generating the pipetting pressure in a pipetting channel, which extends through the pipetting device into the connecting section of the holding device, the air chamber device of the holding device being connected in particular to a suction channel in the pipetting device in which the in the vacuum chamber acting vacuum can be applied.
  • a pressure change device in particular a pump device for generating the pipetting pressure in a pipetting channel, which extends through the pipetting device into the connecting section of the holding device, the air chamber device of the holding device being connected in particular to a suction channel in the pipetting device in which the in the vacuum chamber acting vacuum can be applied.
  • the holding device is a modular component that can optionally be connected — in particular by a user — to the pipetting device and can be separated from it again.
  • a modular design has the advantage that a single holding device can be used with different pipetting devices.
  • both the pipetting device and the holding device can each have their own housing or their own base part, which carries and / or surrounds the further component of the pipetting device or holding device.
  • the pipetting channel of the pipetting device can preferably be fluidly connected to a pipetting channel of the holding device and / or a receiving space of the holding device.
  • Fluidically connectable means a leak-free, tight connection between two air-carrying areas, in particular the pipetting channels.
  • the operating part for operating the clamping device in this modular embodiment is a component of the modular component, in particular — can be operated by a user — integrated into a housing or a base part of the holding device, wherein the base part can be formed by the connecting section.
  • the suction channel between a pumping device of the pipetting device, which is used to create / release the clamping connection of the clamping device of the holding device can preferably be fluidly connected to a further suction channel of the holding device, the further suction channel of the holding device being used to create / release the clamping connection of the clamping device of the holding device. It is also possible that the pipetting channel of the pipetting device can be connected to a suction channel of the holding device.
  • the holding device can have a valve, which can be controlled in particular by the control device or manually by the user, by means of which the suction channel of the holding device and / or the pipetting channel of the holding device can optionally be fluidly connected to the pipetting channel of the pipetting device.
  • the modular component can also be operated with pipetting devices which do not have their own suction channel for controlling the air chamber device, and the modular component can also be operated without its own pump device.
  • the holding device can also have an electrical energy source, in particular a battery or a secondary battery, and preferably an electronic control device.
  • the pipetting container which can be connected to the connecting section is preferably a serological pipette, but can also be a conventional or specially adapted one Pipette tip.
  • Such pipetting containers are in particular disposable products and are preferably made of plastic.
  • the diameters of conventional serological pipettes are not standardized and vary depending on the size, manufacturer and material (glass or plastic).
  • the outer diameters of the end sections of serological pipettes to be connected to the connecting section typically range from 4.5 to 9.0 mm, depending on the manufacturer, for example the outer diameters are as follows in relation to the maximum holding volume of the serological pipettes: 1ml 4.8mm 2ml 6.5mm 5ml 8.1mm 10ml 7.8mm 25ml 9.0mm 50ml 8.3mm 100ml 9.0mm
  • the invention also relates to an automatic laboratory machine for the automated handling of a large number of fluid samples, which has a pipetting device according to the invention and which controls the clamping device of the holding device of the pipetting device, in particular the introduction of the acceptance position and / or the pipetting position, automatically, in particular by computer program.
  • the pipetting device is in particular a multi-channel pipetting device.
  • Such laboratory machines are also referred to as "liquid handling" machines.
  • An example of such a laboratory automat that can be provided with a holding device according to the invention is the liquid handling system of the Eppendorf series epMotion®, for example epMotion® 5070 or epMotion® 5075I from the manufacturer Eppendorf AG, Hamburg, Germany.
  • the invention also relates to a system comprising a holding device according to the invention or a pipetting device having this holding device and a pipetting container which can be connected to the connecting section in an acceptance position of the clamping device of the holding device in order to establish the pipetting position.
  • FIG. 1 shows a schematic side view of a first embodiment of the pipetting device 1 according to the invention, which is provided with a holding device 5 according to the invention.
  • the pipetting device here is a so-called pipetting aid for use with serological pipettes 9.
  • the pipetting device is a device which can be operated manually with one hand and is used in particular in chemical, biological and / or medical laboratories. It has a housing 2 with a handle section 3 and a carrier section 4, at the end of which the downward holding device 5 is located.
  • the pipetting device has a battery 6 and a membrane pump 7 operated thereby.
  • the diaphragm pump 7 on the one hand generates the negative pressure / pressure for pipetting the fluid sample 9a, on the other hand it generates a negative pressure / pressure for operating the air chamber device of the clamping device of the holding device 5, which will be described below.
  • the pipetting container 9 is a serological pipette, the tubular end 9d of which is connected to the connecting section of the holding device 5 by means of the clamping device of the holding device 5.
  • the holding device 5 is set up here as an integral part of a pipetting device. However, it can also be configured simply as a modular component that can be optionally connected to the pipetting device, in particular by a user and can be separated from it again.
  • the pipetting channel of a pipetting device and the pipetting channel of the holding device can be connected in a separable manner, and the suction channel of the pipetting device can also be separably connected to a corresponding suction channel of the holding device.
  • Fig. 2a shows a schematic lateral cross-sectional view of a holding device 100, in an acceptance position of the clamping device, in which a tubular end of a pipetting container 9, in particular a serological pipette, can be accepted by the holding device by placing the tubular end in the receiving space 104 of the connecting section 101 and then the clamping device 102, 103 is actuated to establish the pipetting position.
  • the holding device can be used as the holding device 5 in Fig. 1 be implemented.
  • Fig. 2b shows the holding device 100 in a pipetting position of the clamping device, in which the tubular end of the pipetting container 9, in particular a serological pipette, is non-positively held by the holding device.
  • the holding device 100 for holding a pipetting container in particular a serological pipette, has: a tubular connecting section 101, which is designed to hold the straight tubular end of a pipetting container in a pipetting position by means of a clamp connection on the pipetting device, a clamping device 102, 103, which is arranged on the connecting section 101 and which has a spring device 102 for producing the clamp connection and an air chamber device 103 for releasing the clamp connection, the spring device 102 being set up to exert a spring force F acting radially inwards and perpendicular to the longitudinal axis A, by means of the Pipetting position, the tubular end of the pipetting container is held non-positively on the connecting section.
  • the air chamber 103a of the air chamber device 103 is set up to be deformed in the pipetting position by a negative pressure applied in the air chamber 103a via the connection 103b, as a result of which the spring device 102 is elastically deformed, so that the clamping connection is released.
  • the connecting section 101 is set up to receive the tubular end of the pipetting container in its interior in the pipetting position.
  • the connecting section can be a tubular plastic part that can be connected to a pipetting device 1 or that can be part of a pipetting device.
  • the connecting section 101 has a cylindrical receiving space 104, in which the clamping device 102, 103 is essentially arranged.
  • the air chamber device 103 has an elastically deformable membrane element 103 c.
  • This is an essentially cylindrical elastomer molded part which is arranged coaxially to the axis A with the holding section 101.
  • the upper end 103c.1 of the membrane element 103c is connected to the upper end 101.1 of the holding section 101, in particular the receiving space 104, and the lower end 103c.2 of the membrane element 103c is connected to the lower end 101.2 of the holding section 101, in particular the receiving space 104, connected.
  • an airtight air chamber 103a is formed between the membrane 103c and the holding section 101 and can be subjected to a negative pressure via the closable connection 103b, which is generated by a pump device (not shown) connected to the connection 103b.
  • the holding device can be generated.
  • the membrane can be connected to the holding section 101 by turning the cylindrical end 103c.1, 103c.2 over the ends 101.1 and 101.2 and holding it in this position by means of an elastic tension.
  • the membrane can also be integrally connected to the holding section 101, in particular by means of an adhesive and / or welded connection.
  • the membrane element 103c forms the air chamber 103a together with the holding section.
  • the air chamber device 103 has the connection 103b, with which the negative pressure can be applied in the air chamber device.
  • the clamping device is set up in such a way that a negative inward deflection movement B of the membrane element can be generated, which tensions the spring device 102 due to its elastic deformation.
  • the spring device By ventilating the air chamber 103a, the spring device partially relaxes from its elastically compressed state and generates the force F when the membrane of the membrane element 103c is pushed radially inwards by the spring element and hits the tubular end of the pipetting container. In this state (pipetting position) the spring element is still partially elastically compressed.
  • This membrane acts as a fixing element for fixing the pipetting container to the holding device.
  • the force F results in an axially acting friction force, which holds the pipetting container against displacement along the axis A on the holding device.
  • the spring device 102 has a spring element which effects this spring force F and which is arranged in the air chamber 103a of the air chamber device 103.
  • the spring element here is an essentially tubular foam part made of open-cell polyurethane foam. This is arranged between the membrane and the inside of the holding section 101.
  • the spring element 102 is in the acceptance position in Fig. 2a tensioned or compressed. In the pipetting position, in which the pipetting container is plugged onto the connecting section, the spring element is partially tensioned and less compressed than in the acceptance position.
  • Fig. 3a shows a schematic side view of a holding device 50 according to a first preferred embodiment.
  • the connecting section 51 of the holding device 50 is set up to receive the tubular end of the pipetting container, here a pipette tip 9 ′, in its interior in the pipetting position by the tubular end engaging in the receiving space 54 of the connecting section 51.
  • the clamping device 52 arranged in the receiving space is “deactivated” by applying the vacuum via the intake duct 22, or “activated” by ventilation via the intake duct 22, as is the case in the example of FIG Figures 2a, 2c was explained.
  • the pipetting is carried out via the pipetting channel 21.
  • Fig. 3b shows a schematic side view of a holding device 50 'according to a second preferred embodiment.
  • the connecting section 51 'of the holding device 50' is set up to engage in the pipetting position in the tubular end of the pipetting container, here a pipette tip 9 '.
  • the clamping device 52 ′ arranged on the outside at the lower end of the connecting section is “deactivated” by applying the vacuum via the suction channel 22, or “activated” by ventilation via the suction channel 22. The deactivation creates a vacuum in the air chamber device by means of pump power, which compresses the spring device.
  • the air chamber device is deformed, in particular a membrane is pulled radially inward, so that the connecting section can accept the open tubular end of the pipetting container by the connecting section being able to engage in the open tubular end (acceptance position).
  • the "activation" of the clamping device 52 takes place by ventilating the air chamber device by allowing the spring device to partially decompress in order to expand radially outwards and to press the membrane against the inner wall of the tubular end of the pipetting container.
  • the pipetting is carried out via the pipetting channel 21.
  • the pipetting device 1 shown is used for pipetting a fluid sample (9a) by suction into a pipetting container (9) by means of an air (9b) which is under a pipetting pressure and which in particular has a holding device 100.
  • the pipetting device here has a pump device for generating the pipetting pressure in a pipetting channel, which extends through the pipetting device into the connecting section 101 of the holding device 100, the air chamber device of the holding device being connected to a suction channel in the pipetting device, in which the air chamber device acting vacuum can be applied, the pipetting device having a valve device by means of which either a fluid connection of the suction inlet of the pump device to the pipetting channel or a fluid connection of the suction inlet of the pump device to the suction channel is established.
  • the pipetting device has an electronic control device 8, two operating elements 11, 12 for activating and / or deactivating the pipetting of the fluid sample by suction (11) into the pipetting container or for dispensing (12) the fluid sample from the pipetting container by generating an overpressure.
  • the pipetting device has an operating part 15 for establishing the acceptance position or for releasing the clamping connection of the pipetting container with the holding device, the control device being set up to control the pump device 7 and the valve device 10.
  • Fig. 4 shows a circuit diagram of the fluid power circuit in which the clamping device of the holding device 50, the valve device 10 and the valves 31, 32 are connected to the diaphragm pump 7.
  • the valves 31, 32 are each 3/2-way valves.
  • the control device 8 is set up so that the fluid connection between the pump device and the pipetting channel 21 is closed when the fluid connection between the pump device and the suction channel 22 is open and the pump device 7 is activated, and that the fluid connection between the pump device 7 and the suction channel 22 is closed when the Fluid connection between the pump device 7 and the pipetting channel 21 is opened and the pump device 7 is activated.
  • the pipetting device 1 has a first operating element 11, with which the user controls the pipetting performance of the suction of the fluid sample and thus the speed of the sample reception.
  • the pipetting device has a second control element 12 with which the user controls the pipetting performance of the dispensing of the fluid sample and thus the speed of the dispensing of the sample.
  • the control device 8 is set up and a first 3/2-way valve 31 interacts with the pressure changing device 7, the pipetting channel 21, the suction channel 22 and a ventilation channel 33 open to the environment such that when the first operating element 31 is actuated, the suction channel 22 is closed and the pipetting channel 21 is connected to the pressure changing device-eg pumping device 7-, in particular the input of the pumping device 7, that a negative pressure is generated in the pipetting channel 21, with which the fluid sample can be sucked into the pipetting container 9.
  • the Control device 8 is also set up and a second 3/2-way valve 32 interacts with pressure changing device 7, pipetting channel 21 and suction channel 22 such that when second control element 12 is actuated, suction channel 22 is closed and with the pressure changing device.
  • pump device 7- in particular the outlet of the pump device, is connected to the fact that an atmospheric pressure or excess pressure is generated in the pipetting channel 21, with which the fluid sample can be dispensed from the pipetting container 9.
  • the pipetting device has an operating device by means of which the user can control the clamping device 52, (102, 103) of the holding device 50, 100.
  • An operating element 15 (switch, button, etc.) which is additional to the first 11 and second operating element 12 and which, in particular, switches the valves 31, 32 and in particular the pressure changing device 7, in particular the pump device, is used for the operating device.
  • the control unit 15 is a three-position button, by means of which in particular the three states of the intake duct 22 "vacuum”, "off”, "pressure” can be set.
  • a vacuum can be built up on the control unit 15 during the first actuation, in particular in a time-controlled manner. When the operating part 15 is actuated a second time, the vacuum is then preferably released and the pipetting position is thereby established by means of the spring element 102.
  • FIG. 5 An example of a holding device 200 according to the invention is shown, which is a modular component.
  • the modular holding device can be operated independently of a pipetting device. When used as intended, it is connected to a suitable pipetting device, which is described below with reference to Fig. 6 is described.
  • the holding device are the essential components of the holding device Fig. 2a integrated, which is already detailed in terms of Fig. 2a were explained - which in Fig. 5 The pipetting position, not shown, would then be analogous to that Figure 2b correspond. Repetition is therefore avoided.
  • connection section 201 corresponds to the connection section 101, etc.
  • the modular component 200 can be connected by a user to a pipetting device - and can be separated again from it - by connecting it to the complementary connecting connection of a pipetting device that is adapted to this, via a connection connection 220, which has, for example, a thread or a locking device.
  • the holding device has its own housing 210, which serves as a base part, in which the further components of the holding device are arranged or fastened.
  • the pipetting channel of the pipetting device can be fluidly connected to a pipetting channel 230 of the holding device or the receiving space 204 of the holding device.
  • a sealing device for example silicone, is provided as the sealing device, through which the pipetting channel 230 extends in such a way that at one (lower) end it merges into the receiving space 204 of the holding device, and at the other end it is open in order to communicate with the pipetting channel to be connected to the pipetting device.
  • the hollow body is a hollow cylinder-like part, the lower end of which is fluid-tightly connected, in particular glued, to the upper end 203c.1 of the membrane element 203c and / or the upper end of the connecting section 201.
  • the upper end of the silicone hollow part 216 protrudes beyond the edge of the thread 220 in order to be compressed in the axial direction when the pipetting device and the holding device are connected and thus to serve as a sealing element.
  • the modular component preferably has a filter element 218, which is arranged between the pipetting channel 230 and the pipetting channel of the pipetting device when the modular component 200 is connected to the pipetting device.
  • the filter element is permeable to air and impermeable to liquids. It allows pipetting (suctioning, pressing, or pressure equalization) between the above-mentioned pipetting channels Pipetting and prevents liquid from being sucked into or flowing into the pipetting device if the user accidentally draws in too large a volume or if the pipetting device with the filled pipette accidentally rotates beyond the horizontal.
  • the filter element 218 is here a porous, cylinder-like part that is inserted in the pipetting channel or in the channel formed by the sealing device 216 in a liquid-tight manner, so that in the event of a fault, a liquid does not get into the receiving space 204 or into the pipette section stored there between the Filter element and the inside of the sealing device 216 can flow past.
  • the filter element is impregnated with liquid, the filter element becomes impermeable to air and further pipetting of air or the liquid is prevented, the pipetting channel is blocked and the filter element thus protects the valve device and pump of the pipetting device.
  • the further use of the pipetting system is then only possible again after the filter element 218 has been replaced.
  • the filter element is a disposable item. It preferably consists of a hydrophobic filter material, in particular of hydrophobic polytetrafluoroethylene (PTFE), or has such a material. Such material is not wetted by water vapor and air humidity.
  • PTFE hydrophobic poly
  • the operating part 213 for operating the clamping device is a component of the modular component, in particular — can be operated by a user — integrated into the housing 210 of the holding device.
  • the holding device 200 has an electronic control device which is set up to control the pump 214, in particular as a function of the control parameters influenced by the control panel, by means of which the user switches on the pump in order to open the clamp connection (acceptance position, accordingly) Fig. 2a , 5 ), and by means of which the user activates the clamp connection, preferably by switching off the pump (pipetting position, accordingly Fig. 2b ).
  • the electronic control device has at least one switch to close or open an electrical contact, but it can also have an electronic circuit.
  • the electronic control device can have a battery management system by means of which the charging of the battery 215, which in this case can be charged, is controlled.
  • the holding device has its own pump device 214 for generating the pipetting pressure in the suction channel 203 of the holding device, which is fluidly connected to the air chamber 203a of the holding device and via which the negative pressure acting in the air chamber device can be applied.
  • the pump device is operated by a battery 215, which is also a component of the holding device 200.
  • the clamping device or holding device 200 is a device that can be operated independently, that is to say without the aid of a pipetting device or another device.
  • the pipetting device has a connecting section 320 which is complementary to the connecting section 220 of the holding device 200, that is to say is set up to be connected to it, so that the holding device 200 is firmly connected to the pipetting device and the pipetting channel of the pipetting device is fluid-tight with the pipetting channel 230 the holding device is connected.
  • the connecting section 320 can also be part of an adapter specific for the pipetting device 300, which can be connected to a specific connecting section of the pipetting device 300 in order to connect the holding device 200 via the connecting sections 220, 320 enable.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
EP18195162.5A 2018-09-18 2018-09-18 Dispositif de retenue destiné à retenir un récipient de pipetage d'un dispositif de pipetage Active EP3626343B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FIEP18195162.5T FI3626343T3 (en) 2018-09-18 2018-09-18 HOLDER DEVICE FOR HOLDING THE PIPETTING CONTAINER IN THE PIPETTING DEVICE
EP18195162.5A EP3626343B1 (fr) 2018-09-18 2018-09-18 Dispositif de retenue destiné à retenir un récipient de pipetage d'un dispositif de pipetage
PL18195162.5T PL3626343T3 (pl) 2018-09-18 2018-09-18 Urządzenie przytrzymujące do przytrzymywania pojemnika pipetującego na urządzeniu pipetującym
CN201910813541.9A CN110898869B (zh) 2018-09-18 2019-08-30 用于将吸移容器保持在吸移设备上的保持设备
US16/575,304 US11633731B2 (en) 2018-09-18 2019-09-18 Holding apparatus for holding a pipetting container at a pipetting device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP18195162.5A EP3626343B1 (fr) 2018-09-18 2018-09-18 Dispositif de retenue destiné à retenir un récipient de pipetage d'un dispositif de pipetage

Publications (2)

Publication Number Publication Date
EP3626343A1 true EP3626343A1 (fr) 2020-03-25
EP3626343B1 EP3626343B1 (fr) 2023-12-13

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EP18195162.5A Active EP3626343B1 (fr) 2018-09-18 2018-09-18 Dispositif de retenue destiné à retenir un récipient de pipetage d'un dispositif de pipetage

Country Status (5)

Country Link
US (1) US11633731B2 (fr)
EP (1) EP3626343B1 (fr)
CN (1) CN110898869B (fr)
FI (1) FI3626343T3 (fr)
PL (1) PL3626343T3 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115105995A (zh) * 2022-05-25 2022-09-27 重庆医科大学附属永川医院 一种血液科检验用试管摇混装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4747316A (en) * 1987-03-05 1988-05-31 Millipore Corporation Probe-gripper
JPH06323964A (ja) * 1993-05-13 1994-11-25 Japan Energy Corp 液体試料の分注装置
EP0760255A1 (fr) * 1995-08-31 1997-03-05 Teruaki Itoh Dispositif à buse pour le prélèvement et la distribution d'un échantillon
EP1210978A1 (fr) 1999-05-31 2002-06-05 Bridgestone Corporation Dispositif de pipettes

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4141608C2 (de) * 1991-12-17 1993-12-02 Eppendorf Geraetebau Netheler Pipettiervorrichtung
DE19847869A1 (de) * 1998-10-17 2000-04-20 Hirschmann Laborgeraete Gmbh Pipettiervorrichtung
JP2006208373A (ja) * 2005-01-28 2006-08-10 Molecular Bioproducts Inc バリヤー貫通のためのリブを有するピペットチップを使用した液体採取
CN101850279B (zh) * 2010-06-10 2012-06-13 上海理工大学 协助移液管方便转移液体的装置
DK2633914T4 (da) * 2012-03-02 2020-06-08 Eppendorf Ag Pipetteringsindretning og fremgangsmåde til fremstilling deraf
CN206454669U (zh) * 2017-02-13 2017-09-01 深圳市佳洁农业投资发展有限公司 移液枪组合
CN107321412A (zh) * 2017-08-22 2017-11-07 苏州赛硕拉不锈钢制品有限公司 一种能实现快速卡紧的试管架

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4747316A (en) * 1987-03-05 1988-05-31 Millipore Corporation Probe-gripper
JPH06323964A (ja) * 1993-05-13 1994-11-25 Japan Energy Corp 液体試料の分注装置
EP0760255A1 (fr) * 1995-08-31 1997-03-05 Teruaki Itoh Dispositif à buse pour le prélèvement et la distribution d'un échantillon
EP1210978A1 (fr) 1999-05-31 2002-06-05 Bridgestone Corporation Dispositif de pipettes

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115105995A (zh) * 2022-05-25 2022-09-27 重庆医科大学附属永川医院 一种血液科检验用试管摇混装置
CN115105995B (zh) * 2022-05-25 2023-08-22 重庆医科大学附属永川医院 一种血液科检验用试管摇混装置

Also Published As

Publication number Publication date
CN110898869A (zh) 2020-03-24
PL3626343T3 (pl) 2024-05-06
EP3626343B1 (fr) 2023-12-13
US20200086310A1 (en) 2020-03-19
FI3626343T3 (en) 2024-03-15
CN110898869B (zh) 2022-09-20
US11633731B2 (en) 2023-04-25

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