EP2437890A1 - Dispositif pour transporter un fluide dans un canal d'élément microfluidique - Google Patents

Dispositif pour transporter un fluide dans un canal d'élément microfluidique

Info

Publication number
EP2437890A1
EP2437890A1 EP10725960A EP10725960A EP2437890A1 EP 2437890 A1 EP2437890 A1 EP 2437890A1 EP 10725960 A EP10725960 A EP 10725960A EP 10725960 A EP10725960 A EP 10725960A EP 2437890 A1 EP2437890 A1 EP 2437890A1
Authority
EP
European Patent Office
Prior art keywords
channel
fluid
pressure
pressure source
transport
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP10725960A
Other languages
German (de)
English (en)
Inventor
Lutz Weber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thinxxs Microtechnology GmbH
Original Assignee
Thinxxs Microtechnology GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thinxxs Microtechnology GmbH filed Critical Thinxxs Microtechnology GmbH
Priority to EP21204273.3A priority Critical patent/EP3978133A1/fr
Priority to EP21204270.9A priority patent/EP3978132A1/fr
Publication of EP2437890A1 publication Critical patent/EP2437890A1/fr
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0694—Valves, specific forms thereof vents used to stop and induce flow, backpressure valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance

Definitions

  • the invention relates to a device for transporting a fluid in a channel strand of a microfluidic element, in particular a flow cell.
  • liquids e.g. blood to be examined
  • specific locations e.g. to bring into contact with reagents or / and a detection area.
  • a common task is the separation of a certain amount of liquid from a larger, entered into the flow cell total sample and the further transport of the separated amount of liquid. Often, the separated amount of liquid must be further divided into equal or different sized subsets, the subsets are gozutransport Schl. Occasionally there is also the task of bringing together, via several channels, introduced quantities of different liquids in a single channel for the purpose of further transport of a mixture or sequence of quantities.
  • the amount of liquid filling the channel strand in a plug-like manner is moved by the pressurization against the flow resistance through the channel strand.
  • the lying in the transport direction in front of the liquid region of the channel strand is in communication with a vent.
  • the invention has for its object to provide a new device of the type mentioned, which makes it possible to control transport operations in Mikrofluid- elements more precise and secure than in the prior art and at the same time to reduce the manufacturing cost of the microfluidic elements.
  • the task of this device according to the invention is characterized by a pressure source for pressurizing a front end surface in the transport direction of the channel strand in cross-section filling fluid.
  • the fluid we move the fluid not only by overcoming a resistance caused by friction and capillary forces through the channel strand of the microfluidic element, but also by overcoming an opposing force generated by said pressurization.
  • the pressure applied according to the invention at the front end surface of the fluid in particular a front fluid meniscus, prevents unintentional detachment of small amounts of fluid from the end surface and wetting or remaining parts of the amount of fluid near the delimiting channel walls due to wetting and thus ensures an exact limitation of the transported fluid at the front.
  • the microfluidic element By connecting the channel strand with the pressure source according to the invention instead of with a vent opening, the microfluidic element can be closed to the outside in a fluid-tight manner and prevent environmental contamination by leaking fluid.
  • the pressure source which is preferably a pressurized gas source, may be an integral part of the microfluidic device or e.g. Be part of an operator to which the microfluidic element is coupled.
  • the pressure source comprises a closed space in which a compressed gas, such as air, is compressible by displacement of the front end surface of the transported fluid in the channel strand.
  • a compressed gas such as air
  • the pressure built up in the closed space depending on the position of the end face in the channel string is applied to the end face, and the force generated by this pressure is to be overcome in the transport of the fluid adjacent to the flow resistance.
  • the force used to transport the fluid within the microfluidic element can be of different types. While, for example, an inertial force, in particular a centrifugal force, can be used for displacing the fluid in the channel strand, in a preferred embodiment of the invention the channel strand can be connected to a transport pressure source acting on the fluid in the transport direction.
  • the transport pressure source can also be an integral part of the microfluidic element.
  • the end face, which is rearward in the transport direction, of a quantity of fluid filling the channel strand in a plug-like manner can be filled with a compressed gas, e.g. Air, pressurize.
  • a compressed gas e.g. Air
  • the pressure generated by the pressure source at the front end surface is in a clear functional relationship with the position of the front end surface in the channel strand. This condition is approximately met by the above-mentioned closed space pressure source.
  • a correction factor taking into account the ambient temperature is determined.
  • the transport of the fluid can be interrupted by adjusting the pressure Pl of the transporting pressure source equal to the pressure P2 at the front end surface.
  • An amount of fluid plug-like filling the channel strand can thus be pushed back and forth within a channel strand and positioned at desired locations, eg in reaction areas, detection areas, filters or areas in which it is stored with a reagent stored in the microfluidic element or a test strip known from diagnostics Contact is coming.
  • the Druck ⁇ nctionsch ⁇ r ⁇ kterizing the compressed gas source having the closed space can be advantageously influenced in a desired manner by the fact that the closed space is expandable by the pressurized gas compressed therein.
  • the closed space on one side may have a wall formed by a stretchable film.
  • the closed space of the pressure source can be accommodated in a plate forming the microfluidic element or / and formed by a separate container which can be connected to the plate.
  • the channel strand advantageously has at least one cross-sectional widening to form a chamber, e.g. a detection chamber, a mixing chamber, a reaction chamber o. The like.
  • the chamber may contain dry reagents, e.g. Substances for carrying out a PCR or for capturing analytes of the fluid sample, filters, membranes, test strips, lamellae for mixing, detection means such as optical windows, prisms and electrical conductors, and other means of analysis and synthesis.
  • channel strands In the transport direction several channel strands can run together in a single, connected to a pressure source or connectable channel strand.
  • the multiple channel strands may each be connected or connectable to a transport pressure source so that sequential activation of the transport pressure sources in the single channel strand may generate and transport a sequence or mixture of different fluids.
  • a channel strand can also branch into several, each connected to a pressure source or connectable channel strands, and thus divide a quantity of fluid further without using multiple pressure sources or valves in subsets.
  • the counterpressure applied according to the invention to the front end surfaces of the partial fluid quantities not only allows a uniform distribution of the total quantity in partial quantities, but also the spatial separation of the partial quantities by the transport gas flowing into the fluid streams in the channel strands. As a result, different examinations, analyzes or syntheses can be carried out parallel to one another without mutual influencing of the partial fluid quantities.
  • the complete filling of duct sections with different cross-sectional dimensions is ensured. Especially in the case of cracks and dimensional changes within a channel strand, zones which do not completely flow through or become wetted generally occur, which can lead to the trapping of air bubbles. This is avoided by the invention.
  • FIG. 1 shows a flow cell with a device according to the invention for transporting a fluid
  • FIG. 2 shows the flow cell of FIG. 1 in a detailed view
  • FIG. 3 is a diagram explaining the function of the flow cell of FIG. 1;
  • Fig. 4 shows a modification of the flow cell of Fig. 1
  • Fig. 5 shows an embodiment of an integrated into a microfluidic element
  • Fig. 10 embodiments for branching channel strands, and Fig. 1 1 further embodiments of flow cells with devices according to the invention.
  • a plate-shaped flow cell has an inlet port 1 for a fluid, e.g. a blood test, up.
  • the inlet opening 1 is located in the bottom of a pot-shaped storage vessel 2 molded onto the flow cell.
  • a channel 3 extends, which extends meandering up to a widening 4 and is guided by the widening 4 on to a branching 5. Close to the inlet opening 1 opens into the channel 9, a channel 6, which is in communication with an opening, to which, as will be explained below, an air pressure source can be connected.
  • a channel 8 leading to a vent opens from the channel 3.
  • the cross section of the channel 8 is substantially smaller than the cross section of the channel 3.
  • the channel 3 divides into two branch channels 9 and 9 ', which are symmetrical to the longitudinal central axis of the flow cell and which are divided again at two further branches 10 and 10'.
  • the channel 3 merges into a total of four branches 1 1, I T, 1 1 "and I T".
  • the four branches coincide in the embodiment shown in their embodiment and have identical volumes.
  • Each of the four branches 1 1, I T, 1 1 "and I T" contains a first meandering channel section 12, followed by a channel widening 13.
  • the channel widening 13 contains a dry reagent in the embodiment shown.
  • a second meandering channel section 14 connects.
  • the channel section 14 is followed by a further channel widening 15, which in the relevant embodiment serves as a reaction chamber and contains a further dry reagent, e.g. Reagents by performing a PCR may contain.
  • a third widening 16 follows, which forms a detection chamber.
  • the end of each branch 1 1, 1 1 ', 1 1 ", 1 1'" each forms a chamber 17 with a compared to the volume of the widenings 13, 15 and 16 significantly larger volume.
  • the plate-shaped flow cell consists in the embodiment shown of a plastic plate, in which recesses are incorporated to form the above-described channels and cavities, and a recesses closing, fluid-tight welded or glued to the plastic sheet or foil.
  • the known plastic processing methods in particular injection molding, can be used. Notwithstanding the described structure, a multi-layer substrate and laminated films could be provided. Also suitable as materials are glass, silicon, metal and composite materials. Further processing methods include hot stamping and laser cutting.
  • Various examples of the design of chambers or reaction and detection areas forming channel widenings can be found in the hereby incorporated German patent application 102009015395.0 of the applicant.
  • a fluid sample e.g. a blood sample is input to the storage vessel 2 at the inlet port 1.
  • the channel 3 fills up to the widening 4.
  • the channel 3 can be hydrophilized by plasma treatment or wet-chemical pretreatment.
  • the blood sample could be pressurized, e.g. with a pipette or syringe in the channel 3 bring. This task could also take over an operator facility provided for the flow cell. Air can escape from the channel 3 via the venting channel 8.
  • the widening 4 provides for a limitation of the filling of the channel 3 and thus for a precise dimension of a sample amount, as shown in Fig. 3a.
  • the inlet opening 1 and the channel 8 are closed and the opening 7 is connected to an air pressure source 18, which may be part of an operating device provided for the flow cell.
  • the measured amount of sample can be transported via the widening 4 in the channel 3 to the branch 5, where the sample amount is divided into halves.
  • a further division into halves takes place at the branches 10 and 10 ', so that in the branches 1 1, I T, 1 1 "and I T" in each case a quarter of the measured amount of sample passes.
  • the pressure in the chambers 17 increases by compression during transport of the fluid through the channel 3.
  • the air pressure Pl exerted by the compressed air source 18 must be greater than the respective air pressure P2 in the chambers 17 applied to the front end surfaces 42 of the fluid quantities in the transporting direction.
  • Each position of the partial sample quantities filling the catalytic converter corresponds to a specific pressure P2 in the chambers 17. If the pressure P1 of the compressed-air source 18 is equal to the pressure P2, the partial sample quantities remain in place.
  • the partial sample quantities are located precisely in the channel section 12.
  • the partial sample quantities according to FIG. 3 c can be transferred into the widenings 13, where they each come into contact with a dry reagent.
  • Reduction of the pressure Pl causes a back flow of the subsample quantities in the meandering channel sections 12, where a mixing takes place.
  • a renewed increase in pressure leads the proportion of subsamples through the channel widenings 13 in the next meandering channel section 14.
  • the mixing is completed.
  • the pressure P 1 is further increased, it is transferred to the widenings 15, where in the embodiment shown a reaction takes place, e.g. PCR.
  • the sample tests are completed in widenings 16, where measurements are taken on the processed samples.
  • the compressed air source 18 may have a measuring device for determining the respective pressure P2, which determines its position based on a predetermined relationship between the pressure P2 and the positions of the subsets and, if necessary, automatically controls the transport of the subsets.
  • a flow cell shown in FIG. 4 is largely identical in construction to the flow cell described above. Only the venting channel 8 and the channel 7 having the opening 7 are missing.
  • the sample inlet 1 can be connected to a pressure source and a sample quantity filling the storage vessel 2 can be pressed into the channel 3.
  • the volume of the measured amount of sample thus corresponds approximately to the volume of the storage vessel 2 or a predetermined by the initiator subset. The further processing of the thus sized sample amount is carried out as described above.
  • a pressure source can also be integrated into a flow cell.
  • a pressure source can also be integrated into a flow cell.
  • Fig. 5 is such an integrated pressure source formed by a recess 19 which is covered by a flexible membrane 20.
  • an "air spring” is formed by the closed chamber 17 integrated in the flow cell plate.
  • FIG. 6 shows an exemplary embodiment of an "air spring” with a chamber 22 which is covered by a flexible membrane 23.
  • the membrane which is made of plastic, an elastomer, silicone or TPE, for example, can be widened in such a way Therefore, the dimensions of the "air spring” in the unused state of the flow cell are advantageously smaller than in the operating state.
  • the bulge of the flexible membrane 23 bounding the chamber 22 may e.g. be determined using a simple distance sensor and used to the pressure P2 and thus the
  • the volume of the chamber 22 can be adjusted in the desired manner via the position of the stamp.
  • the stamp can be part of an operator device.
  • FIG. 7 shows a variant of an "air spring” with a separate vessel component 25 that can be attached to a flow cell, wherein a sealing ring 26 surrounds an opening formed on the flow cell plate.
  • a separate vessel component 27 can be plugged onto a flow cell, wherein, for example, a plug-in crown, a press fit and / or a LUER connection can be used.
  • the plate-shaped flow cell itself does not need to have a "spring chamber.”
  • the space required for the integrated chamber can be used in another way 27 an adjustable plug 28, through which the air volume of the vessel portion can be varied so that different conditions for the transport of a fluid within a flow cell can be adjusted.
  • air spring can be part of an operator device and a corresponding connection to the flow cell can be produced according to the connection of FIG. 7 with the aid of an annular seal.
  • FIGS. 1 and 4 show a flow cell with only a single, multi-branching channel strand for the transport of a single fluid supplied via the inlet opening 1
  • a flow cell shown in partial detail in FIG. 9 comprises three channel strands 29, 30 and 31 for transporting different fluids.
  • Each of the channel strands 29, 30, 31 may be connected to an inlet port for the fluid in question and a pressure source. Alternatively, it would be possible to use a pressure source common to all three channel strands.
  • the channel strands 29 to 31 converge at a mixing point 32, from which a single channel 33 extends to a closed chamber 34.
  • a mixing point 32 from which a single channel 33 extends to a closed chamber 34.
  • the channel 33 can be branched again, with branches 35, 35 'in each case communicating with an air spring chamber 36 or 36'.
  • a fluid sequence generated in the channel 33 at the mixing point 32 can be further divided, wherein in the branches 35 and 35 'each have a sequence whose constituents each have half the fluid quantity of the sequence in the channel 33. This may be advantageous to facilitate the successive pressurization of the channels 29 to 31. If fluid sequences are to be generated with particularly small subsets, this would require a very short and accurate pressurization. Passively dividing an initially larger sequence into smaller sequences over the volumes of the sub-strands, their accuracy is decisive and this accuracy can be adjusted very precisely during the production of the microfluidic element by injection molding.
  • Fig. 10b shows a branching channel, with one branch 37 connected to a chamber 38 and another branch 39 connected to a chamber 40.
  • the volume of the chamber 38 is greater than the volume of the chamber 40.
  • Fig. 1 1 shows further embodiments of flow cells, wherein in the embodiment of Fig. 1 1 a, a channel strand with a matrix-like branch and in Fig. 1 1 b, a channel strand is shown with a star-shaped branch.
  • the channel strand has a central inlet opening 41, which at the same time forms a branching point.
  • Fig. 1 1 b is particularly suitable for the transport of fluid by centrifugal force.
  • the flow cell is set in rotation about the inlet opening 41.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un dispositif pour transporter un fluide dans un canal d'élément microfluidique, notamment d'une chambre d'analyse. Selon l'invention, on prévoit une source de pression pour appliquer une pression sur une surface d'extrémité antérieure (42), dans le sens de transport, du fluide remplissant totalement et transversalement le canal. La source de pression comprend de préférence un espace clos (17 ; 22 ; 34 ; 36, 38, 40) dans lequel un gaz sous pression, par exemple de l'air, peut être comprimé en déplaçant la surface d'extrémité antérieure (42) du fluide transporté dans le canal.
EP10725960A 2009-06-05 2010-05-14 Dispositif pour transporter un fluide dans un canal d'élément microfluidique Ceased EP2437890A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP21204273.3A EP3978133A1 (fr) 2009-06-05 2010-05-14 Dispositif de transport d'un fluide dans un canal d'un élément microfluidique
EP21204270.9A EP3978132A1 (fr) 2009-06-05 2010-05-14 Dispositif de transport d'un fluide dans un canal d'un élément microfluidique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE202009008052U DE202009008052U1 (de) 2009-03-23 2009-06-05 Vorrichtung zum Transportieren eines Fluids in einem Kanalstrang eines Mikrofluidelements
PCT/DE2010/000541 WO2010139295A1 (fr) 2009-06-05 2010-05-14 Dispositif pour transporter un fluide dans un canal d'élément microfluidique

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP21204270.9A Division EP3978132A1 (fr) 2009-06-05 2010-05-14 Dispositif de transport d'un fluide dans un canal d'un élément microfluidique
EP21204273.3A Division EP3978133A1 (fr) 2009-06-05 2010-05-14 Dispositif de transport d'un fluide dans un canal d'un élément microfluidique

Publications (1)

Publication Number Publication Date
EP2437890A1 true EP2437890A1 (fr) 2012-04-11

Family

ID=42340358

Family Applications (3)

Application Number Title Priority Date Filing Date
EP21204273.3A Pending EP3978133A1 (fr) 2009-06-05 2010-05-14 Dispositif de transport d'un fluide dans un canal d'un élément microfluidique
EP21204270.9A Pending EP3978132A1 (fr) 2009-06-05 2010-05-14 Dispositif de transport d'un fluide dans un canal d'un élément microfluidique
EP10725960A Ceased EP2437890A1 (fr) 2009-06-05 2010-05-14 Dispositif pour transporter un fluide dans un canal d'élément microfluidique

Family Applications Before (2)

Application Number Title Priority Date Filing Date
EP21204273.3A Pending EP3978133A1 (fr) 2009-06-05 2010-05-14 Dispositif de transport d'un fluide dans un canal d'un élément microfluidique
EP21204270.9A Pending EP3978132A1 (fr) 2009-06-05 2010-05-14 Dispositif de transport d'un fluide dans un canal d'un élément microfluidique

Country Status (4)

Country Link
US (1) US10315197B2 (fr)
EP (3) EP3978133A1 (fr)
DE (2) DE102009015395B4 (fr)
WO (1) WO2010139295A1 (fr)

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* Cited by examiner, † Cited by third party
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US9993818B2 (en) 2013-07-29 2018-06-12 Atlas Genetics Limited Valve which depressurises, and a valve system
US9999883B2 (en) 2013-07-29 2018-06-19 Atlas Genetics Limited System and method for processing fluid in a fluidic cartridge
US10466159B2 (en) 2014-11-28 2019-11-05 Chipcare Corporation Multiplex bead array assay
US10724069B2 (en) 2014-09-29 2020-07-28 Chipcare Corporation Methods and devices for cell detection

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2977757B1 (fr) 2004-04-07 2017-09-13 Abbott Laboratories Chambre jetable pour l'analyse de liquides biologiques
US7731901B2 (en) 2005-10-19 2010-06-08 Abbott Laboratories Apparatus and method for performing counts within a biologic fluid sample
DE102009015395B4 (de) 2009-03-23 2022-11-24 Thinxxs Microtechnology Gmbh Flusszelle zur Behandlung und/oder Untersuchung eines Fluids
JP5709894B2 (ja) * 2009-12-18 2015-04-30 アボット ポイント オブ ケア インコーポレイテッド 生物学的液体分析カートリッジ
WO2012045753A1 (fr) 2010-10-07 2012-04-12 Boehringer Ingelheim Microparts Gmbh Plateforme microfluidique
US9873118B2 (en) 2010-12-30 2018-01-23 Abbott Point Of Care, Inc. Biologic fluid analysis cartridge with sample handling portion and analysis chamber portion
CN108531360A (zh) * 2011-05-27 2018-09-14 不列颠哥伦比亚大学 用于高通量分析的微流控细胞捕获和分析设备
CN105817276B (zh) 2011-08-24 2018-02-06 艾博特健康公司 生物流体样品分析盒
GB2516669B (en) 2013-07-29 2015-09-09 Atlas Genetics Ltd A method for processing a liquid sample in a fluidic cartridge
WO2015105797A1 (fr) 2014-01-07 2015-07-16 Daktari Diagnostics, Inc. Dispositifs, systèmes et procédés d'administration de fluides
WO2017188977A1 (fr) * 2016-04-29 2017-11-02 Kimberly-Clark Worldwide, Inc. Surface pour le transport directionnel de fluide
WO2018122852A1 (fr) 2016-12-29 2018-07-05 Schnell Amit Cartouche devant être utilisée dans des diagnostics in vitro, et procédé d'utilisation associé
KR102104868B1 (ko) * 2017-03-29 2020-04-29 킴벌리-클라크 월드와이드, 인크. 외부 압력 대비 방향성 유체 이송을 위한 표면
US10046322B1 (en) 2018-03-22 2018-08-14 Talis Biomedical Corporation Reaction well for assay device
PL425106A1 (pl) 2018-03-30 2019-10-07 Bacteromic Spółka Z Ograniczoną Odpowiedzialnością Chip mikroprzepływowy
US10820847B1 (en) 2019-08-15 2020-11-03 Talis Biomedical Corporation Diagnostic system
EP4173708A1 (fr) * 2021-10-28 2023-05-03 thinXXS Microtechnology GmbH Élément microfluidique, en particulier cellule d'écoulement, avec réactifsec intégré

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4065263A (en) 1976-04-02 1977-12-27 Woodbridge Iii Richard G Analytical test strip apparatus
US4077845A (en) 1977-04-20 1978-03-07 Miles Laboratories, Inc. Disposable inoculation device and process of using same
DE3541057A1 (de) 1985-11-19 1987-05-21 Kratzer Michael Verfahren und einrichtung zur messung der aggregation der blutplaettchen bzw. der koagulation des blutes
US4999304A (en) 1987-12-28 1991-03-12 Miles Inc. Dynamic braking centrifuge
US5154888A (en) 1990-10-25 1992-10-13 Eastman Kodak Company Automatic sealing closure means for closing off a passage in a flexible cuvette
EP0501796A3 (en) * 1991-03-01 1993-10-06 Biotrack, Inc. Capillary stop-flow junction having improved stability against accidental fluid flow
DE69305046T2 (de) 1992-10-23 1997-04-03 Johnson & Johnson Clin Diag Regelung des Durchflusses in einem eingeschlossenen Behälter
DE4410224C2 (de) 1994-03-24 1996-02-29 Meinhard Prof Dr Knoll Miniaturisiertes Durchfluß-Analysesystem
US5504011A (en) 1994-10-21 1996-04-02 International Technidyne Corporation Portable test apparatus and associated method of performing a blood coagulation test
US5863502A (en) 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US6001307A (en) 1996-04-26 1999-12-14 Kyoto Daiichi Kagaku Co., Ltd. Device for analyzing a sample
DE19648695C2 (de) 1996-11-25 1999-07-22 Abb Patent Gmbh Vorrichtung zur automatischen und kontinuierlichen Analyse von Flüssigkeitsproben
US5922288A (en) 1997-05-29 1999-07-13 Herst; C. V. Taylor Device for isolating a component of a physiological sample
US6012902A (en) * 1997-09-25 2000-01-11 Caliper Technologies Corp. Micropump
GB9804483D0 (en) 1998-03-02 1998-04-29 Central Research Lab Ltd Apparatus for and method of controlling the rate of flow of fluid along a pathway
CN1326549A (zh) 1998-10-13 2001-12-12 微生物系统公司 基于无源流体动力学的流体管路元件
DE19933458B4 (de) 1999-07-15 2015-08-20 Eppendorf Ag Einrichtungen und Systeme zum Handhaben von Flüssigkeitsproben
AU4907101A (en) 2000-02-23 2001-09-03 Caliper Technologies Inc Multi-reservoir pressure control system
US6615856B2 (en) * 2000-08-04 2003-09-09 Biomicro Systems, Inc. Remote valving for microfluidic flow control
US6453928B1 (en) * 2001-01-08 2002-09-24 Nanolab Ltd. Apparatus, and method for propelling fluids
US6875403B2 (en) 2001-02-09 2005-04-05 Microchem Solutions Method and apparatus for reproducible sample injection on microfabricated devices
US20020160518A1 (en) 2001-04-03 2002-10-31 Hayenga Jon W. Microfluidic sedimentation
US6805841B2 (en) * 2001-05-09 2004-10-19 The Provost Fellows And Scholars Of The College Of The Holy And Undivided Trinity Of Queen Elizabeth Near Dublin Liquid pumping system
DE10140699A1 (de) 2001-08-24 2003-03-13 Roche Diagnostics Gmbh Anordnung und Verfahren zur Untersuchung der Fließfähigkeit einer physiologischen Flüssigprobe
US6615866B2 (en) 2001-09-13 2003-09-09 Morrell Incorporated Hydraulic power assembly having a removable top
DE10222478A1 (de) 2002-05-22 2003-12-04 Bartels Mikrotechnik Gmbh Verteilelement für Flüssigkeiten und Gase, Lab-on-a-Cip, Lab-on-a-Card
US7125711B2 (en) 2002-12-19 2006-10-24 Bayer Healthcare Llc Method and apparatus for splitting of specimens into multiple channels of a microfluidic device
CA2513880A1 (fr) 2003-01-21 2004-08-05 Micronics Inc. Procede et systeme de manipulation microfluidique, amplification et analyse de fluides tels les essais de bacteries et tests antiglobuliniques
JP3768486B2 (ja) * 2003-03-20 2006-04-19 株式会社エンプラス 微小流体取扱装置
US7854897B2 (en) 2003-05-12 2010-12-21 Yokogawa Electric Corporation Chemical reaction cartridge, its fabrication method, and a chemical reaction cartridge drive system
DE10339452A1 (de) 2003-08-22 2005-03-17 Institut für Physikalische Hochtechnologie e.V. Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten
DE102004005193B4 (de) 2004-02-02 2006-08-24 Medion Diagnostics Gmbh Vorrichtung zur Separation einzelner Partikel von Partikel-Agglutinationen
US7432106B2 (en) * 2004-03-24 2008-10-07 Applied Biosystems Inc. Liquid processing device including gas trap, and system and method
US8961900B2 (en) * 2004-04-28 2015-02-24 Yokogawa Electric Corporation Chemical reaction cartridge, method of producing chemical reaction cartridge, and mechanism for driving chemical reaction cartridge
US7273590B2 (en) 2004-04-29 2007-09-25 Industrial Technology Research Institute gravity-driven apparatus and method for control of microfluidic devices
US20060002817A1 (en) * 2004-06-30 2006-01-05 Sebastian Bohm Flow modulation devices
US7655470B2 (en) 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
US7491363B2 (en) 2004-07-15 2009-02-17 Applied Biosystems, Llc. Fluid processing device
DE102004046396A1 (de) 2004-09-24 2006-04-13 Land Baden-Württemberg, vertreten durch das Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberg, vertreten durch den Minister Partikelsedimentationsvorrichtung und Verfahren zum Durchführen einer Partikelsedimentation
US20060090800A1 (en) * 2004-10-18 2006-05-04 Applera Corporation Fluid processing device including size-changing barrier
DE102004051573B4 (de) 2004-10-22 2007-03-15 Yokogawa Electric Corporation, Musashino Verfahren zur Behandlung einer Abfallflüssigkeit in Chemischen Reaktions-Patronen und chemische Reaktions-Patrone, in der das Verfahren angewendet wird
US20090047191A1 (en) 2007-06-08 2009-02-19 Gafur Zainiev Closed space disposable micro-reactor and uses thereof
WO2009112030A1 (fr) * 2008-03-12 2009-09-17 Fluimedix Aps Manipulation de liquide régulée
JP2010266203A (ja) 2008-05-20 2010-11-25 Sony Corp 生体情報取得方法及び生体情報取得装置、並びに生理活性物質測定方法及び生理活性物質測定装置
DE102009015395B4 (de) 2009-03-23 2022-11-24 Thinxxs Microtechnology Gmbh Flusszelle zur Behandlung und/oder Untersuchung eines Fluids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9816135B2 (en) 2013-07-29 2017-11-14 Atlas Genetics Limited Fluidic cartridge for nucleic acid amplification and detection
US9908114B2 (en) 2013-07-29 2018-03-06 Atlas Genetics Limited Cartridge, cartridge reader and method for preventing reuse of the cartridge
US9993818B2 (en) 2013-07-29 2018-06-12 Atlas Genetics Limited Valve which depressurises, and a valve system
US9999883B2 (en) 2013-07-29 2018-06-19 Atlas Genetics Limited System and method for processing fluid in a fluidic cartridge
US10724069B2 (en) 2014-09-29 2020-07-28 Chipcare Corporation Methods and devices for cell detection
US10466159B2 (en) 2014-11-28 2019-11-05 Chipcare Corporation Multiplex bead array assay

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DE102009015395B4 (de) 2022-11-24
EP3978133A1 (fr) 2022-04-06
US20120082599A1 (en) 2012-04-05
US10315197B2 (en) 2019-06-11
DE202009008052U1 (de) 2009-08-27
EP3978132A1 (fr) 2022-04-06
WO2010139295A1 (fr) 2010-12-09

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