EP2821138B1 - Cellule d'écoulement avec substance de séchage intégrée - Google Patents

Cellule d'écoulement avec substance de séchage intégrée Download PDF

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Publication number
EP2821138B1
EP2821138B1 EP13175335.2A EP13175335A EP2821138B1 EP 2821138 B1 EP2821138 B1 EP 2821138B1 EP 13175335 A EP13175335 A EP 13175335A EP 2821138 B1 EP2821138 B1 EP 2821138B1
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EP
European Patent Office
Prior art keywords
flow cell
cavity
support element
passage
support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP13175335.2A
Other languages
German (de)
English (en)
Other versions
EP2821138A1 (fr
EP2821138B8 (fr
EP2821138B2 (fr
Inventor
Werer Lutz
Tina Röser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thinxxs Microtechnology GmbH
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Thinxxs Microtechnology GmbH
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Priority to EP13175335.2A priority Critical patent/EP2821138B2/fr
Priority to ES13175335T priority patent/ES2704424T5/es
Priority to DK13175335.2T priority patent/DK2821138T4/da
Application filed by Thinxxs Microtechnology GmbH filed Critical Thinxxs Microtechnology GmbH
Priority to CN201480046983.0A priority patent/CN105517710B/zh
Priority to US14/902,787 priority patent/US10232367B2/en
Priority to PCT/EP2014/064290 priority patent/WO2015001070A1/fr
Publication of EP2821138A1 publication Critical patent/EP2821138A1/fr
Publication of EP2821138B1 publication Critical patent/EP2821138B1/fr
Priority to US16/265,127 priority patent/US10946376B2/en
Publication of EP2821138B8 publication Critical patent/EP2821138B8/fr
Publication of EP2821138B2 publication Critical patent/EP2821138B2/fr
Application granted granted Critical
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Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/163—Biocompatibility

Definitions

  • the invention relates to a microfluidic flow cell, comprising a dry substance disposed in a cavity within the flow cell for interaction with a fluid in the cavity, wherein the cavity is bounded by a recess in a substrate and a cover closing the recess and wherein the flow cell is a separate Carrier element comprises a provided for the arrangement adjacent to the cavity support surface for the dry matter.
  • Microfluidic flow cells which are increasingly being used as a "mini-laboratory" for the analysis or / and synthesis of fluids, in particular in diagnostics, contain reaction substances in liquid or / and solid form, which are to be introduced into the flow cells during the production of the flow cells.
  • a reagent liquid to be dried is brought to the relevant point in an assembly step in which the area provided for receiving the dry reagent within the flow cell, eg a channel or a chamber, is still accessible, ie a carrier liquid having dissolved therein or suspended reagent.
  • the entire, only partially wetted with the reagent liquid component of the flow cell prior to further assembly is subjected to a drying process, which is often associated with a heat treatment for the purpose of acceleration or to protect the reagents and in terms of stability and Resuspend istseigenschaften as freeze drying process.
  • a drying process which is often associated with a heat treatment for the purpose of acceleration or to protect the reagents and in terms of stability and Resuspend istseigenschaften as freeze drying process.
  • the disadvantage is that the component, whose dimensions mostly far exceed those of the area to be dried only, has a large space in a drying chamber. Further, the drying treatment of this component of the flow cell itself affect, especially mounted on sensitive components.
  • the dry substance formed may then be impaired by air contact, in particular humidity, and welding heat or the influence of the adhesive used in the assembly, in many cases hermetically closing the corresponding channel regions of a microfluidic flow cell.
  • a method for introducing a dry substance into a flow cell, as described above, is for example from the EP 2 198 964 B1 out.
  • From the DE 10 2008 021 364 A1 shows a flow cell in which recesses in a substrate are closed by a foil cover.
  • the film cover carries on the inside dry reagents, which face the cavities formed by the covered recesses.
  • a flow cell of the type mentioned starts from the WO 2012/154 306 A1 out.
  • the flow cell has a cavity, which is accessible after opening a hatch or flap and having a recess in a bottom for receiving a cuboid support member. During operation of the flow cell, the hatch or flap must be closed.
  • the invention has for its object to provide a new microfluidic flow cell of the type mentioned with an integrated dry matter, which can be more easily produced than in the prior art without affecting the dry matter or other components of the flow cell through the manufacturing environment.
  • the problem solved flow cell according to the invention is characterized in that in the cavity leads to an outer surface of the flow cell guided, outwardly open passage and that the separate support member from the outside under closure of the cavity and the arrangement of the support surface adjacent to the cavity in the passage can be used.
  • the formation of the dry substance by drying a reagent liquid can be carried out separately from the entire remaining flow cell on a support element, which alone serves to absorb the dry matter and allows the introduction of the dry matter in the flow cell in a final assembly step. Impairment of components of the flow cell by the drying process As well as impairments of the introduced dry reagent by further assembly work on the flow cell accounts.
  • the carrier element can have substantially smaller dimensions than the flow cell, wherein the dimensions of the carrier element are based on the size of the area carrying the dry reagent.
  • the adhesion of the dry substance on its carrier surface-promoting coatings can be advantageously limited to the carrier surface of the carrier element remain so that impairments of welding or adhesive joints are excluded by such coatings.
  • the cavity may form a channel network for the transport, analysis or / and synthesis of a fluid.
  • carrier elements possibly with different dry substances, can be used.
  • the cavity is defined by a recess in a plate-shaped substrate and a recess closing the recess, limited foil-like cover.
  • the passage is formed in the thicker compared to the film-like cover substrate.
  • passage is expediently guided to an outer surface of the flow cell, so that the introduction of the dry substance into the flow cell during its production can take place in a final assembly step.
  • the carrier element is preferably designed such that it can be detachably and / or permanently connected to the flow cell while closing the cavity.
  • the passage is adapted in shape to the shape of the carrier element. Fluid tightness can be achieved in particular by welding or / and gluing, possibly also only by mechanical impressions of the carrier element in the passage.
  • the carrier element expediently completely fills the passage, at least in cross-section, wherein the carrier element and the passage in cross-section preferably have a production-advantageous circular shape.
  • the carrier element tapers towards the cavity while the passage narrows.
  • a tight closure of the cavity in the form of a press fit can be achieved solely by mechanical impressions of the carrier element in the passage.
  • the support member has a portion projecting outwardly of the flow cell, which may serve as a handle portion for ease of manual handling or automated assembly.
  • the projecting portion may overlap the flow cell in the manner of a collar on the outside, whereby an additional sealing of the cavity can be achieved by the collar.
  • the carrier element can be screwed into the passage.
  • the support surface of the support member may be flush or recessed to an adjacent wall surface of the cavity. Alternatively, the support member projects from the adjacent wall surface into the cavity.
  • the carrier surface expediently has a structuring, coating or / and surface modification promoting the adhesion of the dry substance.
  • the carrier element and the carrier surface carrying the dry reagent are preferably made of plastic.
  • the support surface may be formed by a separate, connected to the rest of the support member surface member made of glass, silicon, ceramic or metal and applied by welding or gluing. This is advantageous if surface properties that are realizable for the application of the dry reagent other than by a plastic surface or coating are necessary.
  • the dry reagents include salts, buffers e.g. for cell lysis, magnetic and non-magnetic beads, enzymes, antibodies, DNA fragments, proteins, PCR reagents or, alternatively, cells into consideration.
  • the flow cell shown in detail comprises a plate-shaped substrate 1 with a recess 2, which is covered to form a cavity 3 by a film 4 bonded or / and welded to the substrate.
  • the cavity 3 is part of an in Fig. 1 otherwise not shown channel network of the flow cell, in particular it forms a channel region in which a dry reagent 5 having antibodies, for example, adheres to a channel wall 6.
  • Thenreagenz 5 comes from a before the cover of the recess 2 through the film 4 in a channel or chamber region of the flow cell forming recess 2 by dispensing reagent introduced 7.
  • the entire substrate 1 was a heat treatment or / and lyophilized.
  • a cavity 3 in a flow cell which is, for example, a region of an in Fig. 3 shown channel 9, has a passage opening 10, in which the carrier element 8 with a conical, a support surface 13 for the dry reagent 5 having portion 11 is used under fluid-tight closure of the cavity 3.
  • the support surface 13 forms a part of the wall surface of the cavity 3 after assembly.
  • a fluid transported or processed in the cavity 3 can thus interact with the dry reagent, in particular the dry reagent can be dissolved and resuspended by the fluid.
  • constituents of the fluid such as cells or analytes, can interact and / or bind with the dry reagent by the fluid, possibly several times with change of the transport direction, overflowing the carrier surface.
  • the support element 8 fitted in the passage opening 10 may be glued or welded to the substrate. A beyond the passage opening 10 on the side facing away from the cavity 3 of the substrate 1 protruding portion 12 of the support member 8 serves as the mounting of the support member 8 facilitating handle part.
  • Fig. 1 does not need to form the dry reagent 5 from a reagent liquid 7 as in the example of Fig. 1 to be exposed to drying the entire substrate 1, but only the carrier element 8, which saves space in a drying chamber.
  • the main components of the flow cell, the substrate 1 and the film 4 are not subject to any stress caused by the drying process and finally introduced into the flow cell dry matter 5 no impairment by a subsequent completion of the flow cell with welding of substrate 1 and film 4.
  • Fig. 3 may be present in the channel 9 a plurality of openings for receiving carrier elements 8 with possibly different dry reagents 5 arranged thereon.
  • Fig. 3 serves the meandering channel 9 of the return of the introduced by the support members 8 dry reagents 5 by mutual overflow.
  • the substrate 1 and the film 4 of the flow cell are preferably made of a plastic, in particular both of the same plastic, for which purpose e.g. PMMA, PC, PS, PEEK, PP, PE, COC and COP.
  • the carrier element 8 is preferably a plastic part, which consists in particular of the same plastic as the substrate. The preparation of the substrate and the carrier elements made of plastic is expediently carried out by injection molding.
  • Fig. 4 can remove the dry reagent 5 receiving support surface 13 of the support member 8 to the adjacent wall surface 14 of the cavity 3 is flush or set back to this wall surface.
  • the carrier element 8 with the carrier surface 13 can also protrude into the cavity 3. This may be advantageous for locally generating turbulences in a laminar flow normally present in microchannels by abruptly changing the channel cross section and / or increasing the flow velocity of the fluid in the channel region into which the carrier element 8 is introduced by reducing the channel cross section achieve, for example, to accelerate and control a re-dissolution of the dry reagent.
  • Fig. 5 shows further embodiments of carrier elements 8, which according to Fig. 5a cylindrical and according to Fig. 5b can be cylindrical with a collar 15 engaging behind the substrate 1.
  • Fig. 5c shows an embodiment of a cylindrical, a collar 13 having support member 8 with an external thread 16 which engages in an internal thread in the respective passage opening.
  • the carrier element 8 can be detached from the flow cell, if not in addition to Screw connection even a bonding or welding with the substrate 1 takes place.
  • the solubility can be advantageous if the dry reagent after interaction with the fluid to be separated again from the flow cell and subjected to further analysis.
  • a detachable from the flow cell support member 8 with an extended handle portion 17 shows Fig. 5e ,
  • the carrier element 8 can be pressed under fluid-tight closure of the cavity 3 in the respective passage opening in the substrate 1.
  • the guidance of the carrier element 8 can be improved.
  • Fig. 5d shows a support member 8 with a conical portion and a projecting from a through hole collar 15 which is sealed against the substrate 1 by a ring seal 18.
  • the rotationally symmetrical support elements may have a marking which makes it possible to introduce the support elements in a desired rotational position in the passage.
  • Fig. 6 go embodiments of support elements 8 with differently shaped support surfaces 13, wherein Fig. 6a a carrier element with a recess 19 for receiving a dry reagent 5 has.
  • a support surface 13 is formed with a plurality of receiving recesses in the form of cross-shaped grooves 20 with typical cross-sectional dimensions of 0.01 x 0.01 mm 2 to 1 x 1 mm 2 for a dry reagent.
  • the surface of the support surface 13 can thereby be enlarged in a simple manner, so that either a larger amount of dry reagent 5 can be taken up with the same dimensions of the support element 8 and / or the dry substance can dry more homogeneously than a large drop on a smooth support surface.
  • Fig. 6c shows a receiving surface with a force applied to the support surface by clamping, gluing or welding porous element 21, in which a Can settle dry substance.
  • the porous element 21 forms an enlarged surface for receiving the dry reagent 5.
  • Fig. 6d has a carrier element with a treated carrier surface, wherein the treatment may be, for example, a wet-chemical treatment, a plasma treatment or corona treatment.
  • the treatment can lead to a coating 22, for example by means of plasma polymerization or by PVD process, for example a glass or metal coating.
  • An in Fig. 6e shown carrier component is formed in two parts with a separate surface component 26.
  • the surface component 26 forming the carrier surface does not consist of a plastic, for example, but preferably the rest of the carrier component, but of glass, silicon, metal or ceramic.
  • functionalization ie application of the dry reagent to the support surface, requires such materials, as is the case for example with protein (eg antibody) or nucleic acid based analysis technologies, the use of these materials, which are often significantly more expensive than plastic, is limited. advantageous only to a surface area, with dimensions of 0.5 x 0.5 mm to 5 x 5 mm and thicknesses between 0.1 and 1 mm into consideration.
  • the surface member 26 may be fixed to the remaining support member by means of clamping or by gluing or welding.
  • a multiplicity of carrier elements 8 can be processed simultaneously by arranging the carrier elements 8 on a carrier plate 24 having a row of holes 23 in step 7a.
  • a layer 22 which improves the adhesion of a substance is simultaneously produced on all carrier surfaces 13 of the carrier elements 8.
  • the coating can also cover other, not provided for applying thezelreagenz 5 surface areas of the support member 8.
  • steps 7c and 7d after application of a reagent liquid 7 to the layers 22, a drying treatment takes place so that the dry substance 5 adhering to the layers 22 is deposited on the layers 22.
  • step 7e the finished carrier elements 8 provided with a dry substance 5 can be removed for processing.
  • FIG. 8 Reference is made, where a further embodiment for a carrier element 8 is shown.
  • the carrier element 8 has a carrier surface for a dry substance 5, which is formed by a membrane 27.
  • This membrane can be integrally connected to the rest of the carrier element 8 or formed by a separate, connected to the rest of the carrier element component, which preferably consists of the same plastic as the rest of the carrier element.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Secondary Cells (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (13)

  1. Cellule microfluidique à écoulement, présentant une substance sèche (5) disposée à l'intérieur de la cellule à écoulement dans un espace creux (3), destinée à interagir avec un fluide se trouvant dans l'espace creux (3), l'espace creux (3) étant délimité par un évidement (2) dans un substrat (1) et par un recouvrement (4) fermant l'évidement (2) et la cellule à écoulement comprenant un élément support (8) séparé pourvu d'une surface support (13) pour la substance sèche (5), destinée à être disposée adjacente à l'espace creux (3), caractérisée en ce qu'un passage (10) ouvert vers l'extérieur, guidé vers une surface externe de la cellule à écoulement débouche dans l'espace creux (3) et en ce que l'élément support (8) séparé peut être placé dans le passage (10) depuis l'extérieur tout en fermant l'espace creux (3) et en disposant la surface support (13) adjacente à l'espace creux (3).
  2. Cellule à écoulement selon la revendication 1, caractérisée en ce que l'espace creux (3) forme un réseau de canaux (9) pour le transport, pour l'analyse et/ou pour la synthèse d'un fluide et en ce que, le cas échéant, plusieurs de ces éléments support (8) peuvent être disposés dans plusieurs passages (10).
  3. Cellule à écoulement selon la revendication 1 ou 2, caractérisée en ce que l'évidement (2) est formé dans un substrat (1) en forme de plaque et est fermé par un recouvrement (4) de type feuille et en ce que le passage (10) est formé dans le substrat en forme de plaque.
  4. Cellule à écoulement selon la revendication 3, caractérisée en ce que le passage (10) est guidé vers une surface de plaque du substrat (1) en forme de plaque.
  5. Cellule à écoulement selon l'une quelconque des revendications 1 à 4, caractérisée en ce que l'élément support (8) peut être relié de manière amovible et/ou non amovible à la cellule à écoulement tout en fermant l'espace creux (3) de manière étanche aux fluides.
  6. Cellule à écoulement selon l'une quelconque des revendications 1 à 5, caractérisée en ce que l'élément support (8) remplit complètement, au moins dans la section transversale, le passage (10), l'élément support (8) et le passage (10) présentant de préférence une section transversale circulaire et l'élément support (8) s'effilant vers l'espace creux (3) et le passage (10) se rétrécissant vers l'espace creux.
  7. Cellule à écoulement selon l'une quelconque des revendications 1 à 6, caractérisée en ce que l'élément support (8) présente une partie (12 ; 15 ; 17) en saillie vers l'extérieur par rapport à la cellule à écoulement, la partie s'accrochant le cas échéant derrière la cellule à écoulement, à l'extérieur, à la manière d'un rebord (15).
  8. Cellule à écoulement selon l'une quelconque des revendications 1 à 7, caractérisée en ce que la surface support (13) de l'élément support (8) est disposée de manière affleurante ou en retrait par rapport à une surface supérieure (14) de paroi adjacente de l'espace creux (3) ou en ce que l'élément support (8) dépasse par rapport à la surface (14) de paroi adjacente de l'espace creux (3) à l'intérieur de l'espace creux (3).
  9. Cellule à écoulement selon l'une quelconque des revendications 1 à 8, caractérisée en ce que la substance sèche adhère à la surface support (13) et la surface support (13) présente de préférence une structuration (19-21), un revêtement (22) et/ou une modification de surface favorisant l'adhérence.
  10. Cellule à écoulement selon l'une quelconque des revendications 1 à 9, caractérisée en ce que la surface support (13) pour la substance sèche (5) est formée par un composant (21 ; 26) séparé, relié au reste de l'élément support (8), dont le matériau est différent du reste de l'élément support (8).
  11. Cellule à écoulement selon l'une quelconque des revendications 1 à 10, caractérisée en ce que la surface support (13) pour la substance sèche (5) est formée par une membrane (27) qui ferme une ouverture de passage (28) dans l'élément support (8) vers l'espace creux (3), la membrane (27) étant de préférence transparente et/ou élastiquement déformable par sollicitation via l'ouverture de passage (28).
  12. Procédé pour introduire une substance sèche (5) destinée à interagir avec un fluide dans une cellule à écoulement microfluidique selon l'une quelconque des revendications 1-11, caractérisé en ce que la substance sèche (5) est appliquée sur une surface support (13) d'un élément support (8) séparé et l'élément support (8), présentant la substance sèche (5), est placé dans un passage (10) s'ouvrant vers l'espace creux (3), la surface support (13) délimitant l'espace creux (3) et l'espace creux (3) étant fermé de manière étanche aux fluides.
  13. Procédé selon la revendication 12, caractérisé en ce que pour la formation de la substance sèche (5), un liquide (7) est appliqué sur la surface support (13) et l'élément support (8) est soumis, conjointement avec le liquide (7), à un traitement de séchage, une multitude de tels éléments support étant de préférence traitée simultanément.
EP13175335.2A 2013-07-05 2013-07-05 Cellule d'écoulement avec substance de séchage intégrée Active EP2821138B2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP13175335.2A EP2821138B2 (fr) 2013-07-05 2013-07-05 Cellule d'écoulement avec substance de séchage intégrée
ES13175335T ES2704424T5 (es) 2013-07-05 2013-07-05 Célula de flujo con sustancia seca integrada
DK13175335.2T DK2821138T4 (da) 2013-07-05 2013-07-05 Flydecelle med integreret tørstof
CN201480046983.0A CN105517710B (zh) 2013-07-05 2014-07-04 具有整合的干燥物质的液流电池
US14/902,787 US10232367B2 (en) 2013-07-05 2014-07-04 Flow cell with an integrated dry substance
PCT/EP2014/064290 WO2015001070A1 (fr) 2013-07-05 2014-07-04 Cuve à circulation à matière sèche intégrée
US16/265,127 US10946376B2 (en) 2013-07-05 2019-02-01 Carrier element for introducing a dry substance into a flow cell

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Also Published As

Publication number Publication date
ES2704424T3 (es) 2019-03-18
DK2821138T3 (en) 2019-02-11
US20160167047A1 (en) 2016-06-16
CN105517710B (zh) 2017-04-05
EP2821138A1 (fr) 2015-01-07
US10232367B2 (en) 2019-03-19
EP2821138B8 (fr) 2019-03-06
ES2704424T5 (es) 2022-05-20
CN105517710A (zh) 2016-04-20
EP2821138B2 (fr) 2022-02-09
DK2821138T4 (da) 2022-05-16
WO2015001070A1 (fr) 2015-01-08

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