WO2021013595A1 - Système de laboratoire sur puce comportant au moins une partie fonctionnalisée - Google Patents

Système de laboratoire sur puce comportant au moins une partie fonctionnalisée Download PDF

Info

Publication number
WO2021013595A1
WO2021013595A1 PCT/EP2020/069692 EP2020069692W WO2021013595A1 WO 2021013595 A1 WO2021013595 A1 WO 2021013595A1 EP 2020069692 W EP2020069692 W EP 2020069692W WO 2021013595 A1 WO2021013595 A1 WO 2021013595A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
section
functionalized
lab
chip system
Prior art date
Application number
PCT/EP2020/069692
Other languages
German (de)
English (en)
Inventor
Hannah Bott
Original Assignee
Robert Bosch Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch Gmbh filed Critical Robert Bosch Gmbh
Publication of WO2021013595A1 publication Critical patent/WO2021013595A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Definitions

  • the invention relates to a lab-on-chip system with at least one
  • Microfluidic systems allow the analysis of small amounts of samples with a high level of sensitivity.
  • LoC lab-on-chip
  • Multicomponent fluids such as blood, urine, breath condensate or liquor.
  • blood is the carrier material of the sample to be analyzed in many in-vitro diagnostic tests.
  • several manual purification steps are usually carried out in conventional laboratory operations. This includes the lysis of cells in the starting material, the connection of the sample material, such as DNA, to a filter, as well as the washing and elution of the isolated sample material.
  • the amount of sample present is usually also determined during the purification by measuring the number of cells. This must be carried out in particular if a defined amount of sample is required for the subsequent detection reaction in order to be able to make a quantitative statement.
  • Sample material can be integrated on a LoC system.
  • a LoC system is proposed here with at least one functionalized section that is used for the adhesion of sample components (in particular
  • Sample molecules wherein the functionalized section has a defined length and / or a defined area to a
  • the LoC system is usually a microfluidic system, in particular for receiving and / or processing a (patient) sample.
  • Corresponding microfluidic systems are used especially for handling and / or processing a sample (directly) at the so-called “point-of-care”.
  • the at least one functionalized section has a (pre-) defined length and a (pre-) defined area.
  • the functionalized section can preferably be covered with a layer of surface molecules or
  • Adhesion molecules be provided.
  • Adhesion molecules are usually provided and set up (specifically) to hold the sample molecules to be isolated in the section.
  • sample components are in particular
  • sample components of a certain type or variety that are to be specifically isolated from the sample.
  • the sample components can be sample molecules (of a certain type or variety), for example.
  • the sample components are sample molecules that are to be specifically isolated from the sample.
  • the sample components are sample molecules that are to be specifically isolated from the sample.
  • the sample components are sample molecules that are to be specifically isolated from the sample.
  • Sample components are cells.
  • a defined or known density of surface molecules or adhesion molecules is contained in the layer, in the case of a usually also defined or known layer thickness (and / or defined or known layer volume) from the defined length or area of the functionalized section (directly) on the (largely exact) number of surface molecules or adhesion molecules in the section
  • the functionalized section with its defined length / area is used in particular to determine the number of cells (in particular the maximum number of cells that can be isolated and / or to be isolated).
  • the sample can also be transferred to another medium of known volume.
  • the steps mentioned can either already be implemented in the world-to-chip interface, i.e. in the sample input chamber, or in the adjoining part of the microfluidic system.
  • a particularly advantageous aspect of the LoC system specified here is the defined surface coating of a specified area of the LoC system.
  • System and / or the use of different fluid phases and a suitable fluid flow of these phases.
  • the surface coating can be formed with adhesion molecules that can specifically bind the sample molecules to be separated.
  • inert fluids can be used as the fluid phases as washing buffers and / or carrier reagents which detach the attached sample molecules or, in the case of cells, can lyse them.
  • a suitable fluid flow is generally dependent on the diffusion coefficient of the to be separated
  • Sample molecules and can therefore be adjusted in such a way that enough particles can diffuse to the functionalized surface and be bound there so that it can be saturated with sample molecules.
  • One advantage of the approach described here of a functionalized section of defined length and / or area (to determine the number of cells) is that it can be used universally in many microfluidic systems.
  • One or more (different from one another) surface coatings can be provided in the at least one functionalized section.
  • One or more (different from one another) surface coatings can be provided in the at least one functionalized section.
  • the channel surfaces of the entire fluidic system can be functionalized at certain points and / or used for processing the fluids. Areas that should remain untouched for later analysis can be left out.
  • a sequential processing of the upstream reagents, fluids and the lyophilizate can advantageously be implemented on the LoC. This can enable the required components to be added at the appropriate time.
  • the risk of the disintegration of fragile sample material can furthermore advantageously be reduced. This is in particular because steps for sample preparation can be integrated on the LoC and thus the time-consuming, manual off-chip steps can be omitted.
  • the isolation of the sample material can advantageously be standardized for many samples. Different steps can be on-chip or in the
  • Sample entry chamber can be performed. This can do for many
  • the carrier reagent with which the analytes are released from the functionalized channel section and transported to the reaction chamber has a significantly smaller volume than the original volume of the sample to be analyzed.
  • the analytes be isolated, but also advantageously a volume reduction, which can be advantageous for many microfluidic analyzes.
  • a dilution of the sample material is also possible, in particular if a larger volume of carrier reagent is used.
  • no filter is advantageously required on the chip for the approach presented, its installation in terms of production technology and its
  • Controlling fluidically can be a challenge.
  • the functionalized section is arranged in a flow channel.
  • the flow channel generally describes a (microfluidic) channel through which the sample can flow.
  • Flow channel forms a fluidic connection between at least one input reservoir and at least one output reservoir of the LoC system.
  • the input reservoir can be, for example, an input chamber through which the sample can be introduced or input into the (microfluidic) LoC system.
  • the output reservoir can be an outlet or a
  • Flow channel be at least a factor of ten (10) smaller than a cross section of the at least one input reservoir and / or the at least one output reservoir.
  • the LoC system can have at least one first input reservoir and one second input reservoir and at least one first output reservoir and at least one second output reservoir.
  • the LoC system can also have at least one valve arrangement with which the input reservoirs and / or the output reservoirs can each be specifically connected to the flow channel.
  • the functionalized section is a functionalized one
  • the functionalized surface can be coated with adhesion molecules, for example, so that analytes can adhere there.
  • adhesion molecules for example, so that analytes can adhere there.
  • Fibronectin or poly-D-lysine can be used.
  • At least one first section with a first functionalized surface with a first function and at least one second section with a second functionalized surface with a second function are arranged along the flow channel in a flow direction, the first function and the second Differentiate between functions.
  • the first function and the second Differentiate between functions can also be provided that the
  • Flow channel is connected to an intermediate reservoir between the first section and the second section.
  • the intermediate reservoir can (optionally) be an input reservoir and / or a
  • the functionalized section be formed in an input chamber of the LoC system. If several functionalized sections are provided, it can be provided in this context that at least one of these sections is formed in the input chamber.
  • a method for the quantifiable isolation of sample constituents with a LoC system described here is also proposed, a flow rate of a sample being selected in such a way that adherence of sample constituents in the functionalized section is promoted.
  • the flow rate can be adjusted, for example, so that a minimum number of sample components can adhere.
  • the flow rate is chosen such that the functionalized section is saturated with sample constituents. Furthermore, it can be provided that a diffusion coefficient of sample components in the sample is used when determining the
  • Flow velocity is taken into account.
  • At least one of the following parameters can be taken into account when determining the flow rate or set specifically for the adherence of sample components in the functionalized section:
  • FIG. 2 a possible microfluidic sequence of a sample purification with the system from FIG. 1,
  • Fig. 3 a detailed view of a flow channel for one here
  • FIG. 5 a third example of a lab-on-chip system described here with an associated, exemplary microfluidic process.
  • the lab-on-chip system 1 schematically shows a first example of a lab-on-chip system 1 described here.
  • the lab-on-chip system 1 has a functionalized section 14, which is set up for the adhesion of sample constituents 32, the functionalized section 14 has a defined length and / or a defined area in order to achieve a quantifiable isolation of
  • the functionalized section 14 is exemplary in one
  • Flow channel 2 arranged.
  • the throughflow channel 2 forms, for example, a fluidic connection between two
  • Flow channel 2 is at least a factor of 10 smaller than a cross section of the at least one input reservoir 10, 17 and / or the at least one output reservoir 15, 16.
  • the lab-on-chip system 1 according to FIG. 1 has, for example, a first
  • the lab-on-chip system 1 has a valve arrangement 11 with which the
  • the input reservoirs 10, 17 and / or the output reservoirs 15, 16 can each be specifically connected to the throughflow channel 2.
  • FIG. 1 shows, in particular, a basic example of the presented lab-on-chip system 1.
  • the system 1 has a reservoir for sample input 10, a pump unit 11 and at least one
  • the inlet to the chambers can be closed by a valve 12, 13.
  • a defined part 14 of the microfluidic channel system is functionalized, that is, the channel surface has been coated with adhesion molecules so that analytes can adhere there.
  • adhesion molecules For example, fibronectin or poly-D-lysine can be used for this purpose.
  • the functionalization of the channel section is carried out, for example, in such a way that the area and the concentration of the adhesion molecules on the surface are matched to the requirements of the application and are known.
  • FIG. 2 is subdivided into FIGS. 2A to 2E, which describe an exemplary sequence.
  • FIG. 2A a sample 20 is placed in the input reservoir 10.
  • Pre-storage chamber 17 is given a carrier medium.
  • the sample 20 is pumped through the functionalized channel region 14 into the waste chamber 16.
  • the flow rate is set, for example, so that the functionalized surface is completely saturated with sample molecules.
  • the determining factor for the maximum flow velocity is in particular the diffusion coefficient of the sample to be separated, which in addition to the hydrodynamic radius of the sample molecules is also dependent on the temperature and the dynamic viscosity of the sample liquid. If these parameters are known, the maximum
  • Flow rate can be determined (e.g. modeled), otherwise it can be determined experimentally by determining the purification efficiencies at different flow rates.
  • FIG. 2 also illustrates a method for the quantifiable isolation of sample components 32 with the lab-on-chip system, in which a
  • the flow rate of a sample 20 is selected in such a way that adhesion of sample constituents 32 in the functionalized section is promoted. As described, the flow rate for this can be selected in such a way that the functionalized section 14 is saturated with sample constituents 32.
  • a diffusion coefficient of sample components 32 in sample 20 can be taken into account when determining the flow rate.
  • a radius of the sample components 32 can be used when determining the
  • Flow velocity is taken into account. Furthermore, at least one of the following parameters can also be taken into account when determining the flow rate or can be set specifically for the adherence of sample constituents 32 in the functionalized section 14, 41, 51:
  • the input reservoir 10 is emptied. Some of the analytes from the sample material are bound to the specifically adhesive surface of the functionalized channel region 14, this being saturated, that is to say all adhesive binding sites are occupied with sample molecules. The remaining sample material is located in the waste chamber 16. If necessary, the channel can also be rinsed at this point with a washing buffer, which removes unattached sample molecules and transports them into the waste chamber.
  • the carrier reagent 21 is transferred from the storage chamber 17 via the functionalized channel region 14 into the reaction chamber for the
  • Detection reaction 15 is pumped. Depending on the target and the principle of adhesion, this can be a lysis buffer or a solvent that detaches the analytes again and transports them into the reaction chamber.
  • the carrier reagent 21 is completely pumped out of the storage chamber through the functionalized channel region 14 into the reaction chamber 15. This sets a defined sample volume. Due to the previously ensured saturation of the binding of analytes to the surface of the functionalized channel area, the concentration of sample material in the carrier reagent is now known and a quantifiable one can be used
  • Detection reaction can be carried out.
  • FIG. 3 schematically shows a detailed view of a flow channel 2 for a system described here. It is exemplified in FIG. 3 that the functionalized section 14, 41 can have a functionalized surface with adhesive surface molecules 31, 51. An exemplary sequence is also shown in FIGS. 3A to 3E in FIG.
  • FIG. 3 shows the corresponding detailed views of the functionalized channel region 14 from FIGS. 2A to 2E.
  • cells are isolated from the starting material and lysed in order to carry out a genetic analysis of the DNA.
  • 3A shows a detailed view 14 of the functionalized channel section 30.
  • the inner surface of the channel is functionalized with adhesive surface molecules 31.
  • the sample 20 is pumped from the input chamber through the microfluidic channel with the functionalized section 14 into the waste chamber.
  • the cells 32 contained in the sample are to be isolated for genetic analysis and are attached to the surface by the adhesive molecules
  • Fig. 3C it is shown that with a sufficient concentration of analytes in the sample, the surface of the functionalized channel section after the
  • the carrier reagent 21 for the target connected in the functionalized channel section 14 is then pumped from the pre-storage chamber into the reaction chamber.
  • the carrier reagent contains a lysis medium, so that the bound cells 33 are lysed when rinsing through and the DNA strands 34 get into the carrier medium.
  • the amount of DNA and thus also the concentration in the carrier reagent is known. Will have a significantly lower volume in relation to the original sample material
  • Carrier reagent with DNA is located in the reaction chamber and can be analyzed.
  • FIG. 4 schematically shows a second example of a lab-on-chip system 1 described here.
  • at least one first section 14 with a first functionalized surface with a first function and at least one second section are exemplarily along the flow channel 2 in a flow direction 41 with a second functionalized surface with a second function, wherein the first function and the second function differ from one another.
  • Flow channel 2 can be connected to an intermediate reservoir 15 between the first section 14 and the second section 41.
  • FIG. 4 particularly illustrates the scalability of the concept for samples which contain several targets to be analyzed. There are several
  • the reaction chambers for the detection reaction of the respective target are located in channel sections.
  • An exemplary fluidic sequence for the parallel processing and analysis of several targets from a sample can be broken down as follows: (A) The sample from the input chamber 10 is pumped through the microfluidic channel with the functionalized sections 14, 41,... Into the waste chamber 16. The valves to the reaction chambers 12 are closed, the valves along the channel to the waste chamber 13 are open.
  • the carrier reagent for the target connected in the first channel section 14 is pumped from the first pre-storage chamber 17 into the first reaction chamber 15.
  • This step can be repeated x times for further targets, a storage chamber, a functionalized channel section and a reaction chamber are added.
  • FIG. 5 schematically shows a third example of a lab-on-chip system described here with an associated, exemplary microfluidic process.
  • the functionalized section 51 is formed, for example, in an input chamber 10 of the lab-on-chip system 1.
  • FIG. 5 shows, in particular, how the described microfluidic system and method also directly in one
  • Sample input chamber (a lab-on-chip system) can be implemented.
  • a microfluidic polychamber for example, can be used as a world-to-chip or sample input chamber.
  • FIGS. 5A to 5G for isolating the sample material can be made possible:
  • a sample entry adapter 50 having two chambers is employed.
  • a carrier medium 21 is pre-stored in the pre-storage chamber 17, and in the input chamber 10 there is an area 51 which is filled with adhesives
  • the sample 20 is placed in the input reservoir 10.
  • the sample 20 is drawn into the downstream part of the microfluidic system, e.g. B. via a peristaltic pumping mechanism. A portion 33 of the analytes from the sample is used by the adhesive
  • FIG. 5D the input chamber 10 is emptied, a part 33 of the analytes from the sample material is bound to the specifically adhesive surface of the functionalized area 51.
  • the remaining sample material is located in a waste chamber of the microfluidic system 1.
  • the carrier reagent 21 is now removed from the preliminary storage chamber 17 by an underlayer principle with a non-miscible phase 52, e.g. B. silicone oil, transferred into the input chamber 10.
  • a non-miscible phase 52 e.g. B. silicone oil
  • this allows the amount and concentration of analytes to be set exactly if the amount of analytes is known when the binding of analytes to the surface of the functionalized area is saturated.
  • the carrier reagent 21 for the target connected in the functionalized area 51 is in the input chamber.
  • the carrier reagent contains a lysis medium, so that the bound cells 33 are lysed and the DNA strands 34 get into the carrier medium.
  • the functionalized surface is saturated with cells, the amount of DNA and thus also the concentration in the carrier reagent is known. Will have a significantly lower volume in relation to the original sample material
  • microfluidic system examples include polymers such as polycarbonate (PC), polypropylene (PP), polyethylene (PE), polymethyl methacrylate (PMMA), polydimethylsiloxane (PDMS) or
  • thermoplastic elastomers such as polyurethane (TPU) or styrene block copolymer (TPS), in particular manufactured using high-throughput processes such as injection molding, thermoforming, stamping, laser transmission welding.
  • microfluidic pump device and valves can be implemented, for example, by the pneumatically actuated deflection of a polymer membrane in recesses in a polymer substrate in which the
  • microfluidic channels and reaction chambers can be located.
  • microfluidic system An exemplary dimensioning of the microfluidic system can be given as follows:
  • Possible sample liquids are in particular aqueous solutions
  • Preferred targets are, in particular, sample material contained in the sample liquids, in particular of human origin, e.g. B. bacteria, viruses, certain cells, such as. B. circulating tumor cells, cell-free DNA, proteins or other biomarkers.
  • sample material contained in the sample liquids in particular of human origin, e.g. B. bacteria, viruses, certain cells, such as. B. circulating tumor cells, cell-free DNA, proteins or other biomarkers.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un système de laboratoire sur puce (1) comportant au moins une partie fonctionnalisée (14, 41, 51) conçue pour l'adhésion de constituants d'échantillons (32), la partie fonctionnalisée (14, 41, 51) présentant une longueur définie et/ou une surface définie pour permettre un isolement quantifiable de constituants d'échantillons (32).
PCT/EP2020/069692 2019-07-19 2020-07-13 Système de laboratoire sur puce comportant au moins une partie fonctionnalisée WO2021013595A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102019210697.8A DE102019210697A1 (de) 2019-07-19 2019-07-19 Lab-on-Chip-System mit mindestens einem funktionalisierten Abschnitt
DE102019210697.8 2019-07-19

Publications (1)

Publication Number Publication Date
WO2021013595A1 true WO2021013595A1 (fr) 2021-01-28

Family

ID=71670218

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2020/069692 WO2021013595A1 (fr) 2019-07-19 2020-07-13 Système de laboratoire sur puce comportant au moins une partie fonctionnalisée

Country Status (2)

Country Link
DE (1) DE102019210697A1 (fr)
WO (1) WO2021013595A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829010A (en) * 1987-03-13 1989-05-09 Tanox Biosystems, Inc. Immunoassay device enclosing matrixes of antibody spots for cell determinations
US4945205A (en) * 1984-04-12 1990-07-31 Syntex (U.S.A.) Inc. Chromatographic strip having non-compressed edges
EP1019193A2 (fr) * 1997-03-27 2000-07-19 Biosite Diagnostics Inc. Dispositifs de diagnostic et appareil destine au deplacement regule de reactifs sans membranes
WO2017181186A1 (fr) * 2016-04-15 2017-10-19 Vortex Biosciences, Inc. Puces et cartouches microfluidiques, ainsi que systèmes utilisant des puces et cartouches microfluidiques

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945205A (en) * 1984-04-12 1990-07-31 Syntex (U.S.A.) Inc. Chromatographic strip having non-compressed edges
US4829010A (en) * 1987-03-13 1989-05-09 Tanox Biosystems, Inc. Immunoassay device enclosing matrixes of antibody spots for cell determinations
EP1019193A2 (fr) * 1997-03-27 2000-07-19 Biosite Diagnostics Inc. Dispositifs de diagnostic et appareil destine au deplacement regule de reactifs sans membranes
WO2017181186A1 (fr) * 2016-04-15 2017-10-19 Vortex Biosciences, Inc. Puces et cartouches microfluidiques, ainsi que systèmes utilisant des puces et cartouches microfluidiques

Also Published As

Publication number Publication date
DE102019210697A1 (de) 2021-01-21

Similar Documents

Publication Publication Date Title
EP2072131B1 (fr) Elément microfluide destiné au mélange d'un liquide dans un réactif
DE102013203293B4 (de) Vorrichtung und Verfahren zum Leiten einer Flüssigkeit durch einen ersten oder zweiten Auslasskanal
DE112011102770B4 (de) Mikrofluidische Einheit mit Hilfs- und Seitenkanälen
WO2018086901A1 (fr) Dispositif et procédé permettant un traitement approprié de cartouches fluidiques
EP2560756B1 (fr) Dispositif pour la séparation plasmatique au moyen d'une structure de canal centrale
EP3452217B1 (fr) Dispositif de manipulation de fluide et procédé de manipulation de fluide
EP1846160A1 (fr) Nouveaux porte-echantillons microfluidiques
EP1566215A2 (fr) Plateforme microstructurée et procédé de manipulation d'un liquide
DE10334341A1 (de) Kaskadierte hydrodynamische Fokussierung in Mikrofluidikkanälen
EP1843833B1 (fr) Procede et dispostif de dosage et de melange des petites quantites de liquide, appareil et utilisation
WO2013072110A1 (fr) Élément filtrant microfluidique permettant de séparer des constituants d'un échantillon de fluide biologique
EP2624954B1 (fr) Procédé de nettoyage d'une cavité microfluidique
WO2021013595A1 (fr) Système de laboratoire sur puce comportant au moins une partie fonctionnalisée
EP2525225B1 (fr) Installation et procédé d'analyse de la différentiation de cellules
EP2688670B1 (fr) Système fluidique de remplissage sans bulles d'une chambre de filtration microfluidique
EP2486313B1 (fr) Structure microfluidique et procédé pour le positionnement d'un volume de liquide dans un système microfluidique
WO2014060998A1 (fr) Composant microfluidique intégré pour l'enrichissement et l'extraction de composants cellulaires biologiques
DE102013222283B3 (de) Vorrichtung und Verfahren zur Handhabung von Reagenzien
WO2021063667A1 (fr) Système et procédé de manipulation d'un volume de fluide et de transfert dudit volume dans un système microfluidique
EP2392397A2 (fr) Dispositif destiné au traitement d'un liquide
EP2923760A1 (fr) Cartouche de laboratoire sur puce pour un système microfluidique destiné à analyser un échantillon de matière biologique, système microfluidique destiné à analyser un échantillon de matière biologique et procédé et dispositif d'analyse d'un échantillon de matière biologique
DE102014221309A1 (de) Mikrofluidisches System sowie Verfahren zum Analysieren einer Probenlösung und Verfahren zum Herstellen eines mikrofluidischen Systems zum Analysieren
DE202011108189U1 (de) Vorrichtung und Fluidikmodul zum Erzeugen einer Verdünnungsreihe
DE102021207014A1 (de) Mikrofluidische Vorrichtung und Verfahren zum Betreiben einer mikrofluidischen Vorrichtung
DE102022202864A1 (de) Mikrofluidische Vorrichtung und Verfahren zum Betreiben einer mikrofluidischen Vorrichtung

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20742666

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 20742666

Country of ref document: EP

Kind code of ref document: A1