EP2754495A2 - Système de canaux microfluidique doté d'un dispositif de collecte de bulles et procédé d'élimination de bulles de gaz - Google Patents

Système de canaux microfluidique doté d'un dispositif de collecte de bulles et procédé d'élimination de bulles de gaz Download PDF

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Publication number
EP2754495A2
EP2754495A2 EP13198054.2A EP13198054A EP2754495A2 EP 2754495 A2 EP2754495 A2 EP 2754495A2 EP 13198054 A EP13198054 A EP 13198054A EP 2754495 A2 EP2754495 A2 EP 2754495A2
Authority
EP
European Patent Office
Prior art keywords
membrane
channel system
semipermeable membrane
bubble trap
bubble
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP13198054.2A
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German (de)
English (en)
Other versions
EP2754495A3 (fr
Inventor
Thomas BRETTSCHNEIDER
Jochen Rupp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP2754495A2 publication Critical patent/EP2754495A2/fr
Publication of EP2754495A3 publication Critical patent/EP2754495A3/fr
Withdrawn legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves

Definitions

  • the present invention relates to a microfluidic channel system with a bubble trap device and to a method for removing gas bubbles by means of a bubble trap device in a microfluidic channel system.
  • microfluidic channel systems are used, for example in analytics, in medical diagnostics and, for example, also for the culture of cells or tissues.
  • So-called lab-on-a-chip systems allow complex analyzes or reactions with very small amounts of reagents to be performed on a single chip.
  • the various required reaction and analysis chambers are realized by a channel system.
  • molecular diagnostic laboratory applications can be miniaturized and automated.
  • microfluidic channel systems allow the implementation of modern diagnostic methods, for example, in a doctor's office or even in a patient's home.
  • Gas bubbles in microfluidic systems can arise in different ways. For example, they may be introduced into the system with the reaction liquids, or they may be due to outgassing within the system, such as caused by temperature changes.
  • the known methods and devices for removing gas bubbles from microfluidic channel systems have either the disadvantage that the removal of gas bubbles from the system is insufficient or the bubble trap device is relatively expensive, for example, a vacuum pump for removing the gas volume is required, so that corresponding devices relatively expensive and therefore disadvantageous.
  • the invention is based on the object to provide an apparatus and a method for removing gas bubbles from a microfluidic channel system, the gas bubbles reliably removed from the liquid in a microfluidic channel system while being used with little effort and is very robust during operation.
  • microfluidic channel system with a bubble trap device as is apparent from the claim 1.
  • a method of removing gas bubbles from liquids in a microfluidic channel system is the subject of the further independent claim.
  • Preferred embodiments of the microfluidic channel system with the bubble trap device or the method for removing gas bubbles result from the dependent claims.
  • the microfluidic channel system comprises a bubble trap device, wherein the bubble trap device comprises at least one gas-substantially impermeable and for liquids substantially permeable semipermeable membrane.
  • substantially here is to be understood below that the membrane is largely impermeable to gas and largely permeable to aqueous liquids under normal laboratory conditions.
  • the semipermeable membrane is disposed in the device so that the membrane separates a fluid supply passage and a fluid discharge passage. In the region of the feed channel which adjoins the semipermeable membrane, a bubble trap space is provided in the region of the feed channel which adjoins the semipermeable membrane. This bubble trap space can be made very small and be formed, for example, only by a very small widening of the supply channel.
  • the bubble trap chamber can be acted upon by an overpressure, wherein, for example, in the bubble trap chamber, a pneumatic channel opens, via which the bubble trap chamber can be acted upon by an overpressure.
  • a bubble outlet channel is derived from the bubble trap chamber, via which retained gas bubbles can be discharged.
  • This arrangement is preferably realized as a multilayer structure, for example based on polymers, in particular polycarbonate.
  • the removal of gas bubbles contained in the liquid takes place in such a way that the liquid is guided via the feed channel as far as the semipermeable membrane.
  • the liquid is forced through the semipermeable membrane, wherein due to the membrane properties, the gas bubbles on the side of the supply channel, so in the so-called bubble trap space, are retained.
  • the gas bubbles purified liquid can flow through the membrane and the outlet channel and fed to another fluidic network.
  • the retained gas volume may be withdrawn via the bubble outlet channel or similar means and removed from the system.
  • a semipermeable membrane is particularly suitable a membrane of graphene having said semipermeable properties.
  • a membrane has already been by Nair et al. (Science 335 (2012), pp 442-444 ).
  • the membrane can be made, for example, from graphene oxides.
  • the resulting membrane is not permeable to various gases. For water, however, the membrane is permeable.
  • This membrane was described by Nair et al. generally with regard to suitability for filtration or separation of mixtures.
  • the transfer of the applied overpressure to the liquid can advantageously be effected by means of a further, deflectable, in particular flexible membrane.
  • a flexible polymer membrane for example of a thermoplastic elastomer, is suitable.
  • the deflectable membrane is arranged and fixed to undergo a change in position upon application of an overpressure, which reduces the volume in the bubble trap space, thereby forcing the liquid through the semipermeable membrane.
  • the deflectable membrane is in this case arranged in particular so that it bears against the inner wall of the channel system at least in the area of the bubble trap chamber and is pressed in the direction of the semipermeable membrane when an overpressure is applied.
  • valves are provided for this purpose.
  • the valves may for example be arranged in the external periphery of the bubble trap device, ie in the rest of the channel system, or even within the channel system with the bubble trap device, e.g. in the form of diaphragm valves.
  • the valves can open and close the fluidic paths to the external periphery or to a fluidic network.
  • the bubble outlet channel may be connected via the valve to an open outlet.
  • the inlet channel valve and the outlet channel valve can connect to another fluidic network.
  • the pneumatic access can be connected via a valve with a pneumatic connection.
  • a pressure relief valve is provided for switching the bladder outlet, which automatically opens at a certain, in particular a predetermined and / or adjustable pressure, so that the existing gas bubble can be automatically discharged through the bladder outlet channel.
  • the microfluidic channel system according to the invention has various advantages over known bubble trap devices. For example, only a very small dead volume is required as the bubble trap space for the bubble trap device according to the invention. In contrast, known bubble trap devices require significantly larger dead volumes needed to trap the bubbles. These dead volumes must be taken into account for the total amount of the corresponding liquid, so that in applications with very expensive fluids this significantly increases the cost of the overall system. This disadvantage is not present in the bubble trap according to the invention, since the additional volume of the bubble trap space according to the invention is almost negligible. In addition, the contents of the bubble trap space can be almost completely deflated by deflecting the flexible membrane into the fluidic network so that no liquid remains as an unusable residue.
  • the inventive microfluidic channel system with the bubble trap device does not require a vacuum pump for removing the gas volume.
  • the provision of vacuum in conventional systems adds significantly to the cost and size of the periphery of the channel system, so that conventional systems are burdened in terms of cost and also in terms of portability with significant disadvantages.
  • the system according to the invention generally operates without a vacuum, so that the costs for the production and operation of the microfluidic channel system according to the invention are significantly lower and the size of the entire periphery of the microfluidic channel system according to the invention is also significantly smaller than in conventional systems.
  • the operation of the bubble trap according to the invention requires only the temporary application of an overpressure.
  • an automatic pumping function for removing the retained gas bubbles results from the arrangement according to the invention, so that it is possible to dispense with a further pump.
  • gas bubbles can be removed very quickly and efficiently from a liquid in a microfluidic channel system.
  • the bubble catcher according to the invention the retained gas can be discharged in a few seconds.
  • the removal of gas bubbles in conventional systems takes much longer.
  • the microfluidic channel system according to the invention is suitable for various applications.
  • the channel system according to the invention can be used for cell biological and / or analytical and / or diagnostic devices. It is particularly suitable for so-called lab-on-a-chip systems in which various chemical, biochemical or biological reactions can take place in a very small space.
  • the microfluidic channel system according to the invention for all Microfluidic applications are used, for example, for the cell or tissue culture in small scales.
  • the invention further includes a method for removing gas bubbles from liquids in a microfluidic channel system which employs at least one gas-impermeable and substantially liquid-permeable semipermeable membrane to retain gas volumes on one side of the membrane while liquids carry the membrane can happen.
  • the liquid is pressed by means of overpressure through the semipermeable membrane.
  • existing gas bubbles are retained on the semipermeable membrane and discharged through a bubble outlet channel or similar device.
  • the semipermeable membrane used is preferably a membrane based on graphene, in particular graphene oxides.
  • the liquid is preferably pressed through the semipermeable membrane by means of a further, deflectable, in particular flexible membrane, which is deflected by means of the overpressure.
  • the deflectable membrane is in this case attached in particular to the inner wall of the channel system in the region of the liquid supply to the semipermeable membrane such that it rests against the inner wall and undergoes a change in position when the system is subjected to overpressure, resulting in a reduction of the internal volume of the channel system and in particular of the bubble trap space leads.
  • the retained on the semipermeable membrane gas bubbles are preferably derived by opening the bladder outlet channel, wherein the opening is effected in particular by means of a valve.
  • the valve opens automatically at a certain pressure. This is based on the fact that the pressure in this area increases as soon as there is a gas volume on the inlet side of the semipermeable membrane accumulates, since this affects the liquid transport through the semipermeable membrane. Accordingly, a pressure relief valve which automatically opens at a predeterminable pressure is used as the valve for the bladder outlet channel.
  • the fluid flow in the microfluidic channel system can be driven, for example, by pressure differences in the connected fluidic network (s).
  • the fluid flow in the system is brought about by changes in position of a deflectable, in particular flexible membrane, which, so to speak, performs a pumping function.
  • a deflectable membrane for example, abut against the inner wall of the channel system in the region of the liquid supply to the semipermeable membrane, wherein the application of pressure and / or relieving pressure, a change in position of the membrane is triggered, which displaces the liquid or the space accordingly releases for the liquid.
  • the deflectable membrane which has already been described above.
  • the invention encompasses the use of a semipermeable membrane which is substantially impermeable to gas and substantially permeable to liquids, and which is manufactured on the basis of graphene, in particular graphene oxides, for microfluidic devices.
  • a semipermeable membrane which is substantially impermeable to gas and substantially permeable to liquids, and which is manufactured on the basis of graphene, in particular graphene oxides, for microfluidic devices.
  • Fig. 1 shows a preferred embodiment of a microfluidic channel system according to the invention with a bubble trap device in a plan view, this figure is limited to a schematic representation of the area of the bubble trap device.
  • further channels or channel systems or fluidic networks can be provided which are arranged, for example, on a chip and realize the actual functionalities of the channel system, for example the functionalities of a lab-on-a-chip system.
  • the sectional view shown to a certain extent shows a section of a microfluidic channel system, which illustrates the mode of operation of the bubble trap device according to the invention.
  • the central region 10 indicates the bubble trap space, which comprises a semipermeable membrane as an essential component.
  • the semi-permeable membrane is substantially impermeable to gas and substantially permeable to liquids.
  • the essence of the invention is that the liquid in the microfluidic channel system, which is released according to the invention from gas bubbles, is pressed by this semi-permeable membrane by means of an overpressure, so that gas bubbles are retained on the semipermeable membrane and can be removed from the system.
  • a supply channel 21 for liquids which opens into the region of the bubble trap device 10
  • an outlet channel 22 is provided which leads away the gas-bubble-free liquid from the area of the bubble trap device 10.
  • there is a pneumatic access 31 is provided, via which the area 10 of the bubble trap device can be acted upon by an overpressure.
  • a bubble outlet channel 41 is provided, via which the gas bubbles retained on the semipermeable membrane can be drained off and removed from the system.
  • the channels 21, 22, 41 and the pneumatic access 31 in this embodiment can be opened and closed via valves 51, 52, 54 and 53, so that the bubble trap device can be operated as described below.
  • the illustrated bubble trap device is realized by a multi-layer or multi-layer structure, which in the sectional views of Fig. 2A-F along the line AA 'off Fig. 1 is shown in more detail.
  • the bubble trap space 10 as well as the feed channel 21, the outlet channel 22, the pneumatic access 31 and the bubble outlet channel 41 are formed by the multi-layered structure of the polymer substrates 101, 102 and 103.
  • the basis of this arrangement forms a continuous polymer layer 104.
  • An essential component of the entire bubble trap device is the semi-permeable membrane 11, which is arranged in this embodiment at the base of the bubble trap chamber 10.
  • the valves 51, 52, 53 and 54 are in the sectional views of Fig. 2 not shown.
  • the valves may be located, for example, in the external periphery of the duct system or within the system, for example in the form of diaphragm valves.
  • the valves 51, 52, 53 and 54 open and close the fluidic paths to the external periphery or to a fluidic network associated with the inventive bubble trap means.
  • the pneumatic access 31 is arranged above the bubble trap chamber 10.
  • the bubble trap chamber 10 can be subjected to an overpressure in order to press the fluid through the semipermeable membrane 11.
  • existing gas volumes are retained on the semipermeable membrane 11 and discharged via the bubble outlet channel 41.
  • the bubble outlet channel 41 may be connected to an open outlet that is to be opened or closed with the valve 54 so that the gas bubbles removed from the fluid may be removed from the system.
  • a deflectable and flexible membrane 201 is provided in this embodiment, for example a flexible polymer membrane.
  • This flexible membrane abuts against the wall above the bubble trap space 10 and is movably supported so that it can move into the bubble trap space 10 outside of its fixed areas. This downward movement is effected by the application of positive pressure via the pneumatic access 31.
  • FIG. 2A shows the initial state in which all the valves 51, 52, 53 and 54 are closed.
  • the flexible membrane 201 abuts the upper polymer substrate 103.
  • FIG. 2B the state is shown in which the pneumatic access is opened by opening the valve 53 and subjected to an overpressure. Further, the bubble discharge passage 41 is opened, so that the flexible membrane 201 is deflected and the volume below it is displaced to the outside through the bubble discharge passage 41.
  • FIG. 2C shows the state after the bubble discharge passage 41 has been closed by closing the valve 54.
  • the pneumatic port 31 is set to atmosphere and the supply passage 21 is opened so that the flexible diaphragm 201 relaxes and moves back to its original state.
  • the flexible membrane 201 effectively acts as a pump to drive the fluid flow.
  • the pneumatic access 31 can be subjected to a vacuum at this stage, so that the flexible membrane 201 fits even better to the polymer substrate 103.
  • Fig. 2D illustrates the state in which the liquid 301 is forced through the semipermeable membrane 11.
  • the outlet channel 22 is opened and the pneumatic access 31 is pressurized.
  • the flexible membrane 201 deflects accordingly and displaces the liquid through the semipermeable membrane 11 into the fluidic network, which is connected downstream of the outlet channel 22.
  • Fig. 2E illustrates the case that is sucked with the liquid 301, a gas bubble 401, which is retained when passing the semipermeable membrane 11 on the membrane 11 due to their semi-permeable properties.
  • the bladder outlet channel 41 By opening the bladder outlet channel 41, the gas volume through this channel can be removed from the system and released into the environment, for example, via an open channel.
  • This process is in the Fig. 2F
  • the displacement of the gas volume 401 through the bubble outlet channel 41 is amplified by the applied overpressure, which leads to a deflection of the flexible membrane 201 in the direction of the semipermeable membrane 11.
  • the bubble outlet channel 41 or the valve 54 is initially kept closed during the passage of the liquid through the semi-permeable membrane 11.
  • the pressure on the inlet side of the semipermeable membrane greatly increases because hardly any liquid can flow through the semipermeable membrane 11.
  • the valve 54 which closes the bladder outlet channel 41, is designed as a pressure relief valve which automatically opens at an adjustable pressure, so that the gas volume 401 is automatically discharged through the bladder outlet channel 41.
  • the semipermeable membrane 11 is wetted again with liquid which can penetrate the membrane 11.
  • the pressure in the bubble trap chamber 10 drops again and the outlet valve 54 closes automatically until the passage through the membrane 11 is blocked again by a next bubble.
  • the exemplified mode of operation of the bubble trap device according to the invention also uses the flexible membrane 201 for a pumping function according to the subfigures Figs. 2B and 2C to drive the flow of fluid into the bubble trap device.
  • the fluid flow may be driven solely by pressure differences in the connected fluidic networks, such that the flexible membrane 201 is solely for removing gas bubbles from the fluid according to the subfigures Fig. 2E-F is being used.
  • Fig. 3 shows a further embodiment of the bubble trap device according to the invention, wherein with the previous embodiment, similar elements are denoted by the same reference numerals.
  • a further polymer layer 501 is provided, which is located between the polymer substrate 102 and the polymer substrate 101 in this illustration.
  • This embodiment has advantages in the production of the channel system, since here the semipermeable membrane 11 can be connected to the polymer substrate 102 in a fluid-tight manner, for example by means of laser transmission welding via the polymer layer 501 at the positions 510 (for example annularly).
  • thermoplastic materials can be used for the polymer substrates 101, 102 and 103, for example on the basis of polycarbonate, polypropylene, polyethylene, polymethyl methacrylates, cyclo-olefin polymers (COP) or cycloolefin copolymers ( COC).
  • Elastomers, in particular thermoplastic elastomers, other thermoplastic materials or hot-melt adhesive films can be used for the flexible membrane 201.
  • polymer films for example self-adhesive polymer films.
  • the semipermeable membrane 11 is preferably made of graphene-based materials, in particular graphene oxides.
  • the multilayer structure of the channel system can be advantageously made by laser transmission welding techniques, for example, the polymer substrate 101 can be welded to the semipermeable membrane 11.
  • the polymer layer 501 may be welded to the semipermeable membrane 511.
  • the bonding of the polymer layers with each other and with the flexible membrane 11 is possible and, for example, a welding by means of ultrasound.
  • the microfluidic channel system according to the invention with the bubble trap device can be used in various dimensions.
  • Exemplary dimensions with respect to the thickness of the Polymer substrates are, for example, 0.1 to 10 mm.
  • the diameter of the various channels in the polymer substrates may, for example, be between 200 ⁇ m and 3 mm.
  • the thickness of the flexible membrane 201 and also the thickness of the semipermeable membrane 11 can be selected, for example, between 5 and 500 ⁇ m. For example, ranges between 10 ⁇ 10 to 200 ⁇ 200 mm 2 may be provided as lateral dimensions of the entire channel system.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Degasification And Air Bubble Elimination (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
EP13198054.2A 2013-01-14 2013-12-18 Système de canaux microfluidique doté d'un dispositif de collecte de bulles et procédé d'élimination de bulles de gaz Withdrawn EP2754495A3 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE201310200363 DE102013200363A1 (de) 2013-01-14 2013-01-14 Mikrofluidisches Kanalsystem mit Blasenfängereinrichtung und Verfahren zum Entfernen von Gasblasen

Publications (2)

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EP2754495A2 true EP2754495A2 (fr) 2014-07-16
EP2754495A3 EP2754495A3 (fr) 2015-03-18

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EP13198054.2A Withdrawn EP2754495A3 (fr) 2013-01-14 2013-12-18 Système de canaux microfluidique doté d'un dispositif de collecte de bulles et procédé d'élimination de bulles de gaz

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170368548A1 (en) * 2016-06-27 2017-12-28 M2P-Labs Gmbh Microfluidic chip comprising a functional area, which is covered by a flexible or deformable cover, and microfluidic system
EP3263218A1 (fr) * 2016-06-27 2018-01-03 M2p-labs GmbH Puce microfluidique comprenant une zone fonctionnelle recouverte d'un couvercle souple ou déformable et système microfluidique
CN108217576A (zh) * 2016-12-21 2018-06-29 上海新微技术研发中心有限公司 膜片截止阀及其制造方法
CN115093962A (zh) * 2022-08-24 2022-09-23 翊新诊断技术(苏州)有限公司 基于柔性薄膜的微流控芯片及其在核酸扩增中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6673594B1 (en) * 1998-09-29 2004-01-06 Organ Recovery Systems Apparatus and method for maintaining and/or restoring viability of organs
WO2013086505A1 (fr) * 2011-12-09 2013-06-13 Vanderbilt University Système intégré d'organe sur puce et applications de celui-ci

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NAIR ET AL., SCIENCE, vol. 335, 2012, pages 442 - 444

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170368548A1 (en) * 2016-06-27 2017-12-28 M2P-Labs Gmbh Microfluidic chip comprising a functional area, which is covered by a flexible or deformable cover, and microfluidic system
EP3263218A1 (fr) * 2016-06-27 2018-01-03 M2p-labs GmbH Puce microfluidique comprenant une zone fonctionnelle recouverte d'un couvercle souple ou déformable et système microfluidique
CN108217576A (zh) * 2016-12-21 2018-06-29 上海新微技术研发中心有限公司 膜片截止阀及其制造方法
CN108217576B (zh) * 2016-12-21 2020-05-22 上海傲睿科技有限公司 膜片截止阀及其制造方法
CN115093962A (zh) * 2022-08-24 2022-09-23 翊新诊断技术(苏州)有限公司 基于柔性薄膜的微流控芯片及其在核酸扩增中的应用

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EP2754495A3 (fr) 2015-03-18

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