EP2308589A1 - Structure micro-fluidique - Google Patents

Structure micro-fluidique Download PDF

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Publication number
EP2308589A1
EP2308589A1 EP10180301A EP10180301A EP2308589A1 EP 2308589 A1 EP2308589 A1 EP 2308589A1 EP 10180301 A EP10180301 A EP 10180301A EP 10180301 A EP10180301 A EP 10180301A EP 2308589 A1 EP2308589 A1 EP 2308589A1
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EP
European Patent Office
Prior art keywords
fluid chamber
cross
liquid
section
holding position
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP10180301A
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German (de)
English (en)
Other versions
EP2308589B9 (fr
EP2308589B1 (fr
Inventor
Dr. Jan Claußen
Malte Weniger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut fuer Mikrotechnik Mainz GmbH
Original Assignee
Institut fuer Mikrotechnik Mainz GmbH
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Publication of EP2308589B1 publication Critical patent/EP2308589B1/fr
Publication of EP2308589B9 publication Critical patent/EP2308589B9/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
    • B01F33/3017—Mixing chamber
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/30—Injector mixers
    • B01F25/31—Injector mixers in conduits or tubes through which the main component flows
    • B01F25/314—Injector mixers in conduits or tubes through which the main component flows wherein additional components are introduced at the circumference of the conduit
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/433—Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/433—Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
    • B01F25/4337—Mixers with a diverging-converging cross-section
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3035—Micromixers using surface tension to mix, move or hold the fluids
    • B01F33/30351—Micromixers using surface tension to mix, move or hold the fluids using hydrophilic/hydrophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3035—Micromixers using surface tension to mix, move or hold the fluids
    • B01F33/30352—Micromixers using surface tension to mix, move or hold the fluids using roughness of the surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles

Definitions

  • Microfluidic systems have been the subject of biotechnological research and development in recent years and are increasingly being used in the form of so-called lab-on-a-chip systems, among others. also used for medical diagnosis in point-of-care products.
  • the terms microfluidic system and lab-on-a-chip are used synonymously here.
  • On these microfluidic chip systems previously processed protocols in the laboratory are converted as completely as possible into a microfluidic structure on the Lab-on-a-Chip, so that the protocols are largely automated and run with as few manual interventions as possible.
  • the chip systems are usually used with operator equipment, the operator equipment is equipped with a receptacle for the chip and possibly electrical, fluidic and aktuatorischen interfaces to the chip.
  • Pressure-actuated or pressure-operated in the sense of the microfluidic systems or structures according to the invention means that fluid volumes in the microfluidic systems or structures according to the invention can be driven or driven via a delivery pressure acting from outside the microfluidic system or the microfluidic structure, for example generated by a syringe pump.
  • a passive drive for example a drive acting solely by means of capillary forces, is not possible and provided for the microfluidic systems or structures according to the invention since the cross-sectional dimensions of the microfluidic structures in the microfluidic systems according to the invention are at least partially so large or the surface textures of the microfluidic structures are formed in this way in that there is insufficient capillary pressure for the reliable delivery of fluid through the microfluidic systems.
  • the fluid chamber has a preferably elongated shape, i. its length in the flow direction is greater than its largest cross-sectional dimension of the fluid chamber, more preferably by several times longer than the largest cross-sectional dimension of the fluid chamber.
  • the supply and / or discharge channel respectively opens at a short side or tip into the elongated fluid chamber.
  • the addition port is closable.
  • the pressure difference prevailing in the pressure-driven drive of the liquids can be kept at a lower level in the case of a closed addition opening and it is possible to operate at a pressure lowered in relation to the surroundings.
  • the addition opening is preferably designed to be self-closing, for example by attaching a septum or an elastic lidding film. It is therefore possible to apply sample liquids as second liquid volumes without the risk of the sample liquids emerging from the microfluidic structure according to the invention. Also, a contamination of the interiors of the microfluidic structure according to the invention can be prevented.
  • structures for the secure positioning of the second liquid volume on the holding position comprise in particular two-sided structures, such as stelae on both sides of the feed opening in the fluid chamber, since in this way the holding position for the second liquid volume is formed between these steles and additional holding surfaces for can form the second volume of fluid to the fluid chamber.
  • the feed opening 5 is formed via an opening 54 in the cover sheet 11.
  • the opening 54 can also be generated in this case via a syringe tip in the task of the second fluid volume 42 into the fluid chamber 2.
  • Adhesive structures 7 in the form of a plurality of short steles 71 are arranged in the base plate 10 in the region of the holding position 6 for the second liquid volume 42.
  • the feed opening 5 is formed via an opening 54 in the cover sheet 11.
  • a holding structure 7 in the form of a recess 72 in the region of the holding position 6 for the second liquid volume 42 is attached.
  • an oligonucleotide mixture 142 previously embedded in this microfluidic structure 102 according to the invention via an addition opening is combined with the amplified sample solution.
  • a further process step is at a second, located on the inlet channel 103 opening 112 to the environment, which also with a hydrophobic or non-wettable, semipermeable membrane is closed, over an operator device an overprint abandoned.
  • the entire solution present in the microfluidic structure 102 is thus separated from a supernatant outside that in the microfluidic structure 102.
  • the amplified sample solution is combined via two further microfluidic structures 161, 171 according to the invention for combining two liquid volumes with the required dilution buffer solutions 162, 172 and via an outlet opening 180 from the lab-on-a-chip 100 in a detection device (not shown) on.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
EP10180301.3A 2009-10-06 2010-09-27 Structure micro-fluidique Active EP2308589B9 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102009048378A DE102009048378B3 (de) 2009-10-06 2009-10-06 Mikrofluidische Struktur

Publications (3)

Publication Number Publication Date
EP2308589A1 true EP2308589A1 (fr) 2011-04-13
EP2308589B1 EP2308589B1 (fr) 2013-04-03
EP2308589B9 EP2308589B9 (fr) 2013-08-14

Family

ID=43446578

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10180301.3A Active EP2308589B9 (fr) 2009-10-06 2010-09-27 Structure micro-fluidique

Country Status (3)

Country Link
US (1) US9186638B2 (fr)
EP (1) EP2308589B9 (fr)
DE (1) DE102009048378B3 (fr)

Families Citing this family (10)

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DE102013220445B4 (de) * 2013-10-10 2016-04-07 Robert Bosch Gmbh Auslaufschutzeinheit für eine mikrofluidische Vorrichtung, mikrofluidische Vorrichtung, Verfahren zum Betreiben einer solchen Auslaufschutzeinheit und Verfahren zum Herstellen einer solchen Auslaufschutzeinheit
DE102015204235B4 (de) 2015-03-10 2016-12-15 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Fluidikstruktur mit Halteabschnitt und Verfahren zum Vereinigen zweier Flüssigkeitsvolumina
TWI557163B (zh) * 2015-12-15 2016-11-11 國立清華大學 用於微流道晶片裝置的模具
WO2018183744A1 (fr) 2017-03-29 2018-10-04 The Research Foundation For The State University Of New York Dispositif microfluidique et procédés
US10046322B1 (en) 2018-03-22 2018-08-14 Talis Biomedical Corporation Reaction well for assay device
WO2020093379A1 (fr) * 2018-11-09 2020-05-14 Jiangsu Jitri Micro-Nano Automation Institute Co., Ltd. Dispositifs microfluidiques à base de papier cellulosique nanofibrillé
CN109590035B (zh) * 2018-12-04 2020-09-25 中国农业大学 微流控芯片的制备方法及微流体驱动装置
US11008627B2 (en) 2019-08-15 2021-05-18 Talis Biomedical Corporation Diagnostic system
DE102021110094A1 (de) * 2021-04-21 2022-10-27 Fdx Fluid Dynamix Gmbh Vorrichtung und Verfahren zum Mischen von Fluiden und zum Erzeugen eines Fluidgemisches
CN115283034B (zh) * 2022-08-21 2023-05-16 东北电力大学 一种基于光温耦合响应水凝胶的微流控芯片

Citations (5)

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DE10013311C2 (de) 2000-03-17 2002-08-08 Inst Mikrotechnik Mainz Gmbh Verfahren zum Hydrophobieren der Oberfläche eines polymeren Werkstückes
WO2004012864A1 (fr) * 2002-08-06 2004-02-12 Canon Kabushiki Kaisha Appareil de transfert de liquide et procede pour produire cet appareil
US7247274B1 (en) * 2001-11-13 2007-07-24 Caliper Technologies Corp. Prevention of precipitate blockage in microfluidic channels
WO2008036997A1 (fr) * 2006-09-28 2008-04-03 Fluidyx Pty. Limited Système et procédé de commande de fluides à l'intérieur d'un dispositif microfluidique
EP1932593A1 (fr) 2006-11-22 2008-06-18 FUJIFILM Corporation Puce à micro-canaux et dispositif convergent

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DE10013311C2 (de) 2000-03-17 2002-08-08 Inst Mikrotechnik Mainz Gmbh Verfahren zum Hydrophobieren der Oberfläche eines polymeren Werkstückes
US7247274B1 (en) * 2001-11-13 2007-07-24 Caliper Technologies Corp. Prevention of precipitate blockage in microfluidic channels
WO2004012864A1 (fr) * 2002-08-06 2004-02-12 Canon Kabushiki Kaisha Appareil de transfert de liquide et procede pour produire cet appareil
WO2008036997A1 (fr) * 2006-09-28 2008-04-03 Fluidyx Pty. Limited Système et procédé de commande de fluides à l'intérieur d'un dispositif microfluidique
EP1932593A1 (fr) 2006-11-22 2008-06-18 FUJIFILM Corporation Puce à micro-canaux et dispositif convergent

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GÖTZ MÜNCHOW; DALIBOR DADIC; FRANK DOFFING; STEFFEN HARDT; KLAUS-STEFAN DRESE: "Automated chip-based device for simple and fast nucleic acid amplification", EXPERT REV. MOL. DIAGN., vol. 5, no. 4, 2005

Also Published As

Publication number Publication date
DE102009048378B3 (de) 2011-02-17
EP2308589B9 (fr) 2013-08-14
US9186638B2 (en) 2015-11-17
US20110081275A1 (en) 2011-04-07
EP2308589B1 (fr) 2013-04-03

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