EP2729251B1 - Structure microfluidique avec des cavités - Google Patents

Structure microfluidique avec des cavités Download PDF

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Publication number
EP2729251B1
EP2729251B1 EP12732642.9A EP12732642A EP2729251B1 EP 2729251 B1 EP2729251 B1 EP 2729251B1 EP 12732642 A EP12732642 A EP 12732642A EP 2729251 B1 EP2729251 B1 EP 2729251B1
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EP
European Patent Office
Prior art keywords
cavity
region
liquid
indentation
microfluidic
Prior art date
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Not-in-force
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EP12732642.9A
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German (de)
English (en)
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EP2729251A1 (fr
Inventor
Dirk Kurowski
Oliver Paul
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Microparts GmbH
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Boehringer Ingelheim Microparts GmbH
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0642—Filling fluids into wells by specific techniques
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation

Definitions

  • the invention relates to a microfluidic structure, comprising at least one cavity with at least one inlet opening and at least one outlet opening, wherein the cavity can be filled with a liquid or flowed through by a liquid and within the cavity at least one element is provided which the liquid in the flow within the cavity stops at least temporarily and / or at least partially deflects.
  • Microfluidic structures are components of microfluidic platforms or microfluidic components and essentially comprise cavities and / or channels in which sample liquids to be examined or manipulated can be taken up and transported by suitable means (for example capillary forces, generated pressure differences) to correspondingly provided reaction sites ,
  • the present invention encompasses microfluidic platforms such as, for example, sample carriers, test strips, biosensors, or the like, which may serve to perform individual tests or measurements.
  • biological fluids eg blood, urine or saliva
  • pathogens eg blood, urine or saliva
  • cholesterol blood fat
  • corresponding detection reactions or entire reaction cascades take place on the microfluidic platforms.
  • the biological sample liquid is transported to the appropriate reaction site or the reaction sites by suitable means.
  • a transport of the sample liquid can take place, for example, by means of passive capillary forces (by means of appropriate capillary systems or microchannels) or else by means of an active actuator.
  • active actuators syringe or diaphragm pumps are used, for example, which can be located outside of the microfluidic platform or on this and build up within a micro-fluidic structure consisting of micro channels and micro cavities in particular a corresponding pressure.
  • microfluidic platforms have a sample task on the order of a few millimeters to give up a sample liquid amount on the order of a few microliters, the sample liquid (for example, blood) must be transported via a micro-channel or via a micro-channel system to corresponding cavities, in which, for example chemical reactants are in dried form.
  • sample liquid for example, blood
  • a disadvantage of this structure is that volume actually consumed within the cavity is consumed by the web-like elements.
  • a microfluidic structure or a microfluidic platform for air bubble free filling known. Concretely, there is provided a cavity, with an inlet opening and an outlet opening. In the area of the inlet opening, the Cavity microstructure elements in the form of columns on. This area forms a site with increased capillary force. Due to the increased capillary force, first a complete and air bubble-free wetting of the entrance area of the cavity with sample liquid takes place. Only then is a wetting of the outlet opening facing part of the cavity.
  • EP 0 153 110 A2 shows a microfluidic structure with ribs.
  • a ramp is provided in the cavity, which raises the level of the cavity floor to the level of the outlet opening.
  • Such an arrangement is unsatisfactory for the filling of large, in particular (transverse to the inflow or throughflow direction of the liquid) wide and irregularly shaped cavities.
  • the invention is therefore based on the object to improve a microfluidic structure according to the preamble of claim 1 such that an improved, in particular substantially air bubble-free filling, in particular of large cavities is made possible. This object is achieved with the characterizing features of claim 1.
  • Advantageous developments of the invention can be taken from the subclaims.
  • the invention is therefore based on a microfluidic structure, comprising at least one cavity having at least one inlet opening and at least one outlet opening, wherein the cavity can be filled with a liquid or flowed through by a liquid and within the cavity at least one element is provided, which at the liquid whose flow within the cavity at least temporarily stops and / or at least partially deflects.
  • the at least one element is formed by a depression formed in a wall of the cavity, which has at least one first region at which the liquid is stopped and / or at least partially deflected at least temporarily and at least one second region at which the liquid preferably flows into the depression.
  • the liquid runs immediately upon reaching the second region, that is, without a significant stop into the depression and pulls from a certain filling level the well also in the first area of the well first stopped liquid into the recess.
  • the liquid in the cavity can be controlled so that the cavity is filled evenly and substantially free of air bubbles.
  • This is also possible with large, in particular wide and irregularly shaped cavities which, for example, have a filling volume of the order of magnitude of about 10 ⁇ l to 10 ml.
  • said wall of the cavity may be, for example, a bottom of the cavity. But there are also any other walls of the cavity conceivable.
  • the second region is formed by a ramp-like transition, which, starting from a bottom level of the cavity, passes over to a bottom level of the depression.
  • This ramp-like transition ensures in a simple manner that the sample liquid at this point runs into the depression without a stop and fills it.
  • the ramp-like transition starting from a boundary edge of the recess with a bottom plane of the cavity forms an angle of about 10 degrees to 60 degrees, more preferably of about 45 degrees.
  • the second region could also be formed by a "smooth" transition, for example by a convex or concave rounding.
  • a notch-like structure (seen in plan view of the depression) is conceivable.
  • the first region is expediently formed by a boundary edge of the depression on which the converging, forming the boundary edge Walls occupy an angle which is less than 120 degrees, preferably approximately between 95 degrees and 70 degrees, more preferably at about 90 degrees.
  • the first region forms a capillary stop in a very reliable manner, at which the inflowing liquid is first stopped or deflected.
  • the at least one recess is elongated, wherein the at least one first region facing an inflowing liquid and the at least one second region of an inflowing liquid is remote.
  • the inflowing liquid can be controlled such that it initially reaches the first area, is stopped there, deflected and preferably reaches the second area (without a noteworthy stop) into the depression and fills it.
  • the recess may be formed in plan view, for example, approximately rectangular. But it can also be different in plan view, for example, be arcuate. This may be expedient, for example, if the cavity to be filled is likewise curved in its longitudinal extension.
  • a further advantageous embodiment of the inventive idea provides that a plurality of depressions are provided, which are arranged starting from side walls of the cavity, mutually.
  • the at least one first region extends approximately over the entire length of one longitudinal side of the at least one depression and the at least one second region only over a portion of the length of another longitudinal face.
  • the invention also relates to a microfluidic platform with at least one microfluidic structure according to at least one of claims 1 to 7.
  • a microfluidic platform can be produced inexpensively and meets high demands on a process-reliable, in particular air bubble-free filling of the existing cavities.
  • the microfluidic structure 1 comprises a cavity 10, which has a filling volume of about 15 ⁇ l.
  • the cavity 10 is unevenly shaped and provided with an inlet opening 11, which connects the cavity 10 with a filling channel 16.
  • the filling channel 16 itself may be connected to an unspecified numbering filling opening (for example, a sample task area).
  • the cavity 10 is provided with an outlet opening 12 which, for example, releases the fluidic connection to a ventilation channel 17.
  • a capillary stop 24 is also provided in the usual way.
  • the cavity 10 may be connected via an outlet opening to a further microchannel 18 (indicated by dashed lines) if a liquid is to be transported through the cavity 10, for example into a further cavity (not shown).
  • the cavity 10 is a comparatively large cavity having dimensions of about 12 mm in width, 36 mm in length and about 1.5 mm in depth.
  • Each recess 13 has in plan view approximately a rectangular appearance with a length L and a width B.
  • the recesses 13, starting from longitudinal sides of the cavity 10, are mutually arranged.
  • each depression 13 has a first region 14 which faces an inflowing liquid F (cf. Fig. 4 ) and at which the inflowing liquid F is at least temporarily stopped and / or at least partially deflected.
  • each depression 13 is provided with a second region 15, at which an incoming liquid F runs into the depression 13 without stopping.
  • the cavity 10 is closed by a cover 21 (for example, a glued foil) and has a bottom 19.
  • a cover 21 for example, a glued foil
  • Each recess 13 has a bottom 20.
  • the first region 14 (capillary stop) is formed by a boundary edge 22 of the recess 13, at which the converging, the boundary edge 22 forming walls occupy an angle ⁇ , which is 90 degrees. Deviating from the embodiment, of course, other angles are conceivable, which may be greater or less than 90 degrees.
  • the second region 15 is formed by a ramp-like transition R, which, starting from the bottom level 19 of the cavity 10, to a bottom level 20 of the recess 13 passes.
  • the ramp-like transition R starting from a boundary edge 23 of the recess 13 with the bottom plane 19 of the cavity 10 forms an angle ⁇ of approximately 45 degrees. Again, angles greater or less than 45 degrees are conceivable.
  • the second area 15 does not necessarily have to be formed by a ramp-like transition, but other configurations are also conceivable. So is in Fig. 3 indicated that the second area, for example, by a “smooth" transition, such as by a concave (15 ") or convex (15"') rounding can be formed.
  • the liquid F is first stopped at the first region 14 or the boundary edge 22 and deflected ( Fig. 4b ).
  • the liquid F continues to the first region 14 of the second recess 13 and thereby also to the second region 15 of the first recess 13, whereby the liquid F via the second region 15, the first recess 13 fills (see dashed lines indicated arrow in FIG Fig. 4c ).
  • the liquid F is then stopped and deflected again at the first region 14 of the second depression 13 and a complete filling of the cavity 10 takes place, first leaving the second depression 13 (see FIG Fig. 4d ).
  • the second depression 13 is also filled via the second region 15 (ramp-like transition R).
  • the liquid front of the liquid F now extends to the first region 14 of the last depression 13 (FIG. Fig. 4e ).
  • the liquid F is again initially stopped and deflected until it subsequently reaches the second region 15 of the last depression 13 and, starting there, fills it.
  • the filling process extends to the capillary stop 24 in the region of the outlet opening 12 and essentially takes place without air inclusions (air bubbles) (cf. Fig. 4f ).
  • the second region 15 does not extend over the entire length L of a depression 13, but only constitutes part of this length. Furthermore, the region 15 also assumes a width which is significantly smaller than the width B of the entire depression 13. In particular, the width of the region 15 is preferably less than half the width B of the depression 13. This makes it possible, with sufficient filling function Area 15 well exploit the volume of the recess 13.
  • the regions 15 are positioned on the longitudinal sides of the depressions 13 facing away from the inflowing liquid F, it is also conceivable to provide such regions at least partially on the transverse sides of the depressions 13 (cf. dashed lines 15 'in FIG Fig. 1 ). It is also conceivable to provide several such areas at one depression (see also paragraphs 43 in FIG Fig. 6 ).
  • a second embodiment 3 for a microfluidic structure of a microfluidic component 4 is shown.
  • the microfluidic structure 3 comprises a cavity 30, with Each recess 31 is in turn equipped with a first region 32 in the form of a stop edge (capillary stop), which faces the flow direction S of an inflowing liquid.
  • a second region 33 in the form of a ramp is again provided on the longitudinal side of the depression 31 facing away from an inflowing liquid, the region 33 extending over an entire length L of the depression 31.
  • the width of the region 33 is in turn only about a maximum of half a width B of the recess 31.
  • a meandering flow of an incoming liquid is modeled by the mutual arrangement of the recesses 31.
  • a microfluidic structure 5 can be seen on a microfluidic component 6, which (in contrast to the preceding exemplary embodiments) has a curved cavity 40 viewed in the inflow direction S of a liquid.
  • each recess 41 has a longitudinal extent L and extends over this length L curved. Furthermore, it can be seen that each depression 41 is in turn provided with a first region 42 in the form of a stop edge (comparable to region 14 of the first embodiment) and on the longitudinal side facing away from an inflowing liquid, each having two regions 43 in the form of a ramp (comparable to FIG the area 15 in the first embodiment).
  • a microfluidic structure 7 of a microfluidic component 8 can be seen, in which, within a cavity 50 unlike the embodiments according to the invention, no depressions, but webs 51 are attached.
  • the webs 51 starting from longitudinal sides of the cavity 50, are mutually arranged and intended to meander a flow of an inflowing liquid (not shown) and thus enable a largely bubble-free filling of the cavity 50.
  • the webs 51 extend from a bottom 53 of the cavity 50 starting up to a cavity 50 upwards final cover 52 zoom.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micromachines (AREA)

Claims (6)

  1. Structure microfluidique (1, 3, 5), comprenant au moins une cavité (10, 30, 40) avec au moins une ouverture d'entrée (11) et au moins une ouverture de sortie (12), dans laquelle la cavité (10, 30, 40) peut être remplie d'un fluide (F) ou traversée d'un tel fluide et au moins un élément (13, 31, 41) est prévu à l'intérieur de la cavité (10, 30, 40), lequel stoppe au moins temporairement et dévie au moins par zone le fluide (F) dans son écoulement (S) à l'intérieur de la cavité (10, 30, 40), caractérisée en ce que l'au moins un élément (13, 31, 41) est formé par un évidement (13, 31, 41) ménagé dans une paroi (19) de la cavité (10, 30, 40), lequel (13, 31, 41) présente au moins une première zone (14, 32, 42), qui est formée par une arête de limitation (22) de l'évidement (13), au niveau de laquelle les parois convergentes formant l'arête de limitation (22) forment un angle (β), qui est inférieur à 120°, de manière particulièrement préférée d'environ 90° et la première zone (14, 32, 42) forme un arrêt capillaire, au niveau duquel le fluide (F) est arrêté au moins temporairement et dévié au moins par zone et l'au moins un élément (13, 31, 41) présente au moins une deuxième zone (15, 15', 15", 15"', 33, 43), qui est formée par une transition de type rampe (R), qui, partant d'un niveau de fond (19) de la cavité (10) passe à un niveau de fond (20) de l'évidement (13), au niveau duquel le fluide (F) pénètre dans l'évidement (13, 31, 41).
  2. Structure microfluidique (1, 3, 5) selon la revendication 1, caractérisée en ce que la transition de type rampe (R), partant d'une arête de limitation (23) de l'évidement (13) forme avec le plan de fond (19) de la cavité (10) un angle (α) d'environ 10° à 60°, de manière particulièrement préférée d'environ 45°.
  3. Structure microfluidique (1, 3, 5) selon l'une quelconque des revendications 1 ou 2, caractérisée en ce que l'au moins un évidement (13, 31, 41) est réalisé allongé, dans laquelle l'au moins une première zone (14, 32, 42) est tournée vers un fluide entrant (F) et l'au moins une deuxième zone (15, 33, 43) est opposée à un fluide entrant (F).
  4. Structure microfluidique (1, 3) selon l'une quelconque des revendications 1 à 3, caractérisée en ce que plusieurs évidements (13, 31, 41) sont prévus, qui sont agencés alternativement en partant de parois latérales de la cavité (10, 30, 40).
  5. Structure microfluidique (1, 5) selon l'une quelconque des revendications 1 à 4, caractérisée en ce que l'au moins une première zone (14, 32, 42) s'étend environ sur toute la longueur (L) d'un côté long de l'au moins un évidement (13, 31, 41) et l'au moins une deuxième zone (15, 33, 43) s'étend seulement sur une partie de la longueur (L) d'un autre côté long.
  6. Plateforme microfluidique (2, 4, 6) avec au moins une structure microfluidique (1, 3, 5) selon au moins l'une quelconque des revendications 1 à 5.
EP12732642.9A 2011-07-05 2012-07-02 Structure microfluidique avec des cavités Not-in-force EP2729251B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP12732642.9A EP2729251B1 (fr) 2011-07-05 2012-07-02 Structure microfluidique avec des cavités

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11172775 2011-07-05
PCT/EP2012/062863 WO2013004673A1 (fr) 2011-07-05 2012-07-02 Structure microfluidique comportant des creux
EP12732642.9A EP2729251B1 (fr) 2011-07-05 2012-07-02 Structure microfluidique avec des cavités

Publications (2)

Publication Number Publication Date
EP2729251A1 EP2729251A1 (fr) 2014-05-14
EP2729251B1 true EP2729251B1 (fr) 2018-11-14

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EP12732642.9A Not-in-force EP2729251B1 (fr) 2011-07-05 2012-07-02 Structure microfluidique avec des cavités

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US (2) US20140227148A1 (fr)
EP (1) EP2729251B1 (fr)
JP (1) JP6098020B2 (fr)
WO (1) WO2013004673A1 (fr)

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JP6240785B2 (ja) * 2013-12-20 2017-11-29 スリーエム イノベイティブ プロパティズ カンパニー サンプルの濃縮及び検出のためのシステム及び方法
WO2015184343A1 (fr) * 2014-05-30 2015-12-03 Absolute Exhibits, Inc. Film de finissage en moule thermodurci
TWI499637B (zh) * 2014-06-20 2015-09-11 Ind Tech Res Inst 發泡體及包含其之發光裝置
GB201617869D0 (en) 2016-10-21 2016-12-07 Blacktrace Holdings Limited A microfluidic device

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4271119A (en) * 1979-07-23 1981-06-02 Eastman Kodak Company Capillary transport device having connected transport zones
US4618476A (en) 1984-02-10 1986-10-21 Eastman Kodak Company Capillary transport device having speed and meniscus control means
DE10313201A1 (de) * 2003-03-21 2004-10-07 Steag Microparts Gmbh Mikrostrukturierte Trennvorrichtung und mikrofluidisches Verfahren zum Abtrennen von flüssigen Bestandteilen aus einer Flüssigkeit, die Partikel enthält
DE10360220A1 (de) 2003-12-20 2005-07-21 Steag Microparts Gmbh Mikrostrukturierte Anordnung zur blasenfreien Befüllung zumindest eines Systems zur Ableitung von Flüssigkeiten, Vorrichtung mit einer solchen Anordnung und Befüllungsverfahren
DE102004027422A1 (de) * 2004-06-04 2005-12-29 Boehringer Ingelheim Microparts Gmbh Vorrichtung zur Aufnahme von Blut und Abtrennung von Blutbestandteilen
EP1616619A1 (fr) * 2004-07-17 2006-01-18 Tecan Trading AG Dispositf et procédé ayant une influence sur bulles d'air dans une chambre hybridation
ATE503578T1 (de) * 2005-01-27 2011-04-15 Boehringer Ingelheim Micropart Verwendung einer vorrichtung zur untersuchung von probenflüssigkeit
DE102005017653A1 (de) * 2005-04-15 2006-10-19 Boehringer Ingelheim Microparts Gmbh Vorrichtung und Verfahren zur Manipulation einer Flüssigkeit
JP4685611B2 (ja) * 2005-12-02 2011-05-18 株式会社エンプラス 微小流体装置

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

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US9409171B2 (en) 2016-08-09
US20150251182A1 (en) 2015-09-10
JP2014521056A (ja) 2014-08-25
US20140227148A1 (en) 2014-08-14
JP6098020B2 (ja) 2017-03-22
WO2013004673A1 (fr) 2013-01-10
EP2729251A1 (fr) 2014-05-14

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