EP3541516B1 - Dispositif de prélèvement, d'expulsion et de déplacement de liquides - Google Patents

Dispositif de prélèvement, d'expulsion et de déplacement de liquides Download PDF

Info

Publication number
EP3541516B1
EP3541516B1 EP17808819.1A EP17808819A EP3541516B1 EP 3541516 B1 EP3541516 B1 EP 3541516B1 EP 17808819 A EP17808819 A EP 17808819A EP 3541516 B1 EP3541516 B1 EP 3541516B1
Authority
EP
European Patent Office
Prior art keywords
chamber
liquid
fluidic
channel
microfluidic system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP17808819.1A
Other languages
German (de)
English (en)
Other versions
EP3541516C0 (fr
EP3541516A1 (fr
Inventor
Christian Moche
Richard Klemm
Claudia Gärtner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microfluidic Chipshop GmbH
Original Assignee
Microfluidic Chipshop GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microfluidic Chipshop GmbH filed Critical Microfluidic Chipshop GmbH
Publication of EP3541516A1 publication Critical patent/EP3541516A1/fr
Application granted granted Critical
Publication of EP3541516B1 publication Critical patent/EP3541516B1/fr
Publication of EP3541516C0 publication Critical patent/EP3541516C0/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0638—Valves, specific forms thereof with moving parts membrane valves, flap valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent

Definitions

  • the invention relates to a device for receiving, dispensing, diluting or moving liquids and for adding liquid components, which can also be referred to as a microfluidic system.
  • the device can also be referred to as a chip.
  • the external pumps for manipulating lab-on-a-chip systems require a fluidic interface, which requires additional components and which, like all fluidic interfaces, involves the risk of leakage.
  • Syringe pumps integrated directly into fluidic systems avoid a fluidic interface to the outside, but require another element, the plunger, to move liquids.
  • Diaphragm valves offer the advantage that they do not require a fluidic interface or additional components and only require a preformed recess and a movable cover for actuation. These are designed in such a way that they can be operated both pneumatically and mechanically. These diaphragm valves are usually operated using a corresponding control gear.
  • handling usually takes place via external pumps and requires a device to control the system.
  • WO 2015/084458 A2 discloses a reagent dispensing device comprising: a first substrate having a first surface, the first substrate including one or more first chambers disposed within the first substrate; a first resistor unit disposed adjacent the surface of the first substrate and fluidly connected to at least one of the one or more first chambers, the first resistor unit including a first reagent.
  • WO 2015/077412-A1 , WO 2010/091246 A2 , DE 10 2009 032744 A1 and WO 2007/057744 A2 disclose fluidic systems with chambers, channel systems and flexible regions.
  • the present invention combines all handling steps, including the pre-storage of reagents, on a component that can also be operated manually.
  • the object of the invention is to be able to take up, dispense, dilute, transport and/or mix liquids both manually, i.e. without additional aids, and with appropriate devices. This should preferably be possible in fluidic systems without an external pump or suction device, preferably also manually.
  • a special feature of the system is that liquids can be taken up and dispensed multiple times and that desired volumes of the liquid taken up or dispensed can be precisely controlled.
  • a fluidic system comprising a structured component with a chamber and a channel system, which are sealed in a fluid-tight manner by a component, the chamber being fluidically connected to the outside world via the channel system and a fluidic interface.
  • the device has a flexible or moveable area that can be moved into the chamber area or beyond a level of the chamber.
  • the level of the chamber is the upper limit of the chamber on the side to the chamber, i.e. the underside of the component closing the chamber. Due to the movement of the flexible area, liquids or gases can be taken up or released through the fluidic interface or moved in the fluidic system.
  • the movable area can be moved manually or with an appropriate operating device. One option is to push the flexible area in or move it up to different positions.
  • the fluidic system preferably has an interface for a liquid reagent reservoir.
  • the configuration of the component closing the structured component as a film is particularly advantageous, the film being the movable component at the same time due to its intrinsic flexibility.
  • the liquid taken up is diluted or the reagents are supplied by emptying a liquid reservoir which is connected to the structured component and which can be configured as a blister.
  • the liquid intake and liquid removal can be influenced by the external geometry of the fluidic interfaces.
  • the volume can be defined by the corresponding outlet geometry of the fluidic interface, and this volume definition can be further influenced by a surface modification of the fluidic interface.
  • a further fluidic system comprising a structured component with a chamber and a channel system which are sealed with a further component, the chamber being fluidically connected to the outside world via the channel system and a fluidic interface.
  • the flexible area is formed by the walls of the chamber.
  • pressing the chamber laterally also allows the liquid to move or the compression effect can be intensified by the flexible chamber walls.
  • a further fluidic system comprising a structured component or structured component and a further component which seals the chamber and channel system tightly and the chamber is connected to the outside world via the channel system and the fluidic interface.
  • the structured component is designed in such a way that the chamber floor is flexible and can be pressed in or expanded.
  • the base can be designed to be particularly flexible and production by means of two-component injection molding is possible, so that a flexible component can be injected together with another component.
  • the base material of the structured component can also be sufficiently flexible to ensure the functionality of the component. An assembly of the flexible area in the structured component is also possible.
  • the chamber can be connected to a fluidic interface via a further channel system, it being possible for one of the fluidic interfaces to be closed with a cap.
  • the closure with a cap also prevents liquid leakage at this point.
  • valves for example capillary stop valves, which act by changing the capillary diameter, allows defined volumes to be taken up.
  • a valve function is preferably created by local modification of the surface or the function of existing geometrically acting valves is further reinforced by a surface modification in the valve area.
  • the fluidic system includes a venting option for the chamber, which can be via an additional channel communicating with the outside world or a gas-permeable membrane, and this venting device can optionally be closed.
  • the fluidic system preferably contains an inlet channel that has a passive stop function, for example a capillary stop valve, a channel narrowing or a corresponding surface modification, and either via capillary action, which can be enhanced by surface modifications in the area to be filled, or by one brought about by the moving components Change in chamber volume absorbs a defined amount of liquid.
  • a passive stop function for example a capillary stop valve, a channel narrowing or a corresponding surface modification, and either via capillary action, which can be enhanced by surface modifications in the area to be filled, or by one brought about by the moving components Change in chamber volume absorbs a defined amount of liquid.
  • the fluidic system contains an additional reagent reservoir. This can be formed as a blister, for example.
  • Dry reagents are preferably introduced into the structured component, which can be absorbed by the fluids flowing through and mixed with them.
  • a reagent is preferably placed at a defined point, which colors the liquid flowing over and thus indicates that the position at which the reagent is placed has been reached, and thus indicates that a specific volume or a residence time has been reached.
  • a magnification function is preferably introduced into the structured component at a defined position, which takes place e.g. in the form of a lens integrated into the structured component, in order to be able to better track the reaching of certain positions in the channel system through the liquid and also to be able to read color reactions as indicator reactions better can.
  • Longer channel elements are also preferably introduced into the course of the fluid as flow limiters in order to enable controlled fluid intake and discharge.
  • the reagent reservoir is in the form of a blister.
  • the reagent reservoir preferably has a blister seat which has pointed elements which pierce the blister which is seated above and which is connected in a liquid-tight manner.
  • This embodiment has a flap which, via guide elements in the blister seat, allows the flap to be inserted in a defined manner and thus allows a defined volume dosage.
  • the volume dosing can also take place in several stages thanks to the special design of the guide elements.
  • the liquid-tight closure of the fluidic interface for liquid intake is useful, for example via a cap.
  • the cap can also be provided with a transport element, e.g.
  • the cap can also have a flexible area which, after it has been put on, can be pressed in or pulled out in order to move the liquid in the channel or in the channel system. When pressed, the liquid is pushed further into the channel. When the flexible area is pulled out, liquid is conveyed out of the channel in the direction of the fluidic interface. This also allows small movements to be generated.
  • the fluidic system has a long channel leading to the chamber.
  • This long channel is particularly advantageous since it allows the rate at which liquid is taken up to be adjusted and reagents can be introduced into the channel which resuspend optimally as they are carried along in the channel for a long time.
  • the long channel has additional expansions towards the chamber. This embodiment is particularly advantageous since reagents can be prepackaged in the expansions and improved mixing can take place due to a different flow profile.
  • the fluidic system contains a cavity or detection chamber for optical readout and/or for reaction, which can preferably also have different depths. It is particularly advantageous here that optical detection can be carried out directly and the dynamic range can also be increased if the detection chamber is designed with several depths.
  • the fluidic system contains a lateral flow strip, the filling of which is made possible by the operation of the chamber.
  • a lateral flow strip the filling of which is made possible by the operation of the chamber.
  • One design variant includes a ventilation membrane, another a ventilation channel.
  • the possibility of liquid aspiration, which can be operated manually, with the direct option of reading out via the lateral flow strip, is particularly advantageous.
  • Specifically targeted venting options allow the combination of the vacuum-driven flow achieved through the chamber with the subsequent liquid movement through the suction effect of the lateral flow strip.
  • the fluidic system contains more than one chamber, which are connected to one another via a channel system and can be arranged in one or more levels. It is particularly advantageous that forwarding and pushing back and forth as well as active mixing are made possible by changing the chamber volume through the flexible elements.
  • the fluidic system contains attachments on the flexible components, which are either located outside the chamber or extend into the chamber.
  • attachments on the flexible components which are either located outside the chamber or extend into the chamber.
  • the fluidic system has reagents that are placed in the chamber. It is particularly advantageous here that the chamber not only serves to move the liquid, but that the chamber volume can be used directly for dissolving, reacting and mixing reagents. In particular, dry reagents that are provided allow a particularly advantageous use of the chamber.
  • the cap for emptying the blister is connected directly to pressure elements for moving the flexible area, possibly also realized in one piece.
  • mixing is possible by moving elements introduced into the chamber, such as balls or rods, which can also be magnetic.
  • the mixing can be additionally enhanced by or structural elements in the structured component.
  • a particular advantage here is that the simple structure of the system allows particularly effective mixing in the chamber.
  • mixing takes place in the chamber by manually moving the fluidic system.
  • a particular advantage here is that the simple structure of the system allows manual use.
  • mixing takes place in the chamber by means of an on-board mixing mechanism. It is particularly advantageous here that efficient mixing can take place.
  • the channel systems themselves contain alignment marks, or alignment marks are attached next to, below or above the channel system, which enable volume information to be given.
  • This marking is particularly advantageous, similar to a ruler, as it allows the user to read the volume taken up or dispensed and to end or continue taking up or dispensing volumes in order to add, dispense or move defined volumes.
  • fluidic interfaces are provided on the structured component, which point in different directions, for example perpendicular to the plane of the fluidic system or starting from the fluidic system at a specific angle. It is particularly advantageous here that a special geometry allows liquids to be applied or dispensed to specially shaped surfaces or vessels.
  • the intake or delivery of the liquids is controlled via membrane valves, see FIG. This is particularly advantageous since it allows fluid to be taken up or released individually at different fluidic interfaces as a result of the movement of the flexible elements in the chamber.
  • a special design is the integration of passive valves in the individual distribution channels in order to ensure uniform filling and thus uniform liquid transport and thus, for example, the delivery of the same volumes.
  • the uptake and delivery of the liquids is controlled via rotary valves.
  • the rotary valves preferably have a rotary valve seat (28a) and a rotating rotary valve body (28b) with a connecting channel that connects the different parts of the channel system. This is particularly advantageous since it allows fluid to be taken up or released individually at different fluidic interfaces as a result of the movement of the flexible elements in the chamber.
  • the fluidic system is designed as a microfluidic system.
  • the structured component is preferably and essentially made of plastic.
  • the entire component can be produced as a plastic film, for example.
  • a flexible plastic such as silicone or TPE that is introduced into the other components, or a movable mechanical element made of any material.
  • the fluidic system is also referred to as a thumb pump, since the flexible component can be operated particularly easily with the thumb.
  • the present invention describes a fluidic system with a chamber, which has a flexible or movable part, usually the base or lid, but also movable walls in special embodiments, which, by lifting or pressing down, allows the intake, delivery, displacement, dilution or Allows mixing of liquids or gases connected to the chamber via at least one channel or port.
  • the chamber and the movable part are designed in such a way that a predetermined but adjustable volume of the chamber is displaced by a movement of the movable part from its starting position.
  • predetermined volumes can be taken up or released when the movable part is returned to another position or to the starting position in the chamber.
  • the volume is predetermined by the properties of the fluidic system or can be adjustable by designing the fluidic system according to the invention.
  • Figures 1a to 1c show an embodiment of the fluidic system.
  • Figures 1a and 1c show a plan view of the fluidic system
  • Fig. 1b shows a cross-sectional view of the fluidic system.
  • the fluidic system has a structured component 1 with a chamber 2 , the chamber 2 being connected to a channel system 3 .
  • the structured component 1 is essentially flat or plate-like.
  • the structured component 1 has a first main side and a second main side, which face each other in parallel.
  • the chamber 2 and the channel system 3 are formed on the surface of the structured component 1 on the first main side.
  • the chamber 2 and the channel system 3 are let into the surface of the structured component 1 on the main side.
  • the chamber 2 and the channel system 3 therefore represent a depression on the surface of the structured component 1.
  • the first main side is, for example, a top side
  • the second main side is, for example, a bottom side of the structured component 1.
  • the structured component can be cuboid, for example.
  • the structured component 1 can also be disk-shaped.
  • the patterned member can take any shape as long as it is substantially flat.
  • the structured component 1 can be designed as a platform, for example.
  • the structured component 1 can also be referred to as a structured component 1 .
  • the structured component 1 can be of flat design.
  • the chamber 2 or the channel system 3 therefore has an upper side which corresponds to the upper side of the structured component 1 .
  • An underside of the chamber 2 or of the channel system 3 is formed within the structured component 1 .
  • the underside of chamber 2 can also be referred to as chamber floor 7 .
  • the interior of the chamber 2 is formed between the top of the chamber 2 and the bottom.
  • the chamber 2 or the channel system 3 can be designed as a depression in the structured component, e.g. on the upper side or the underside of the structured component 1.
  • the chamber 2 and the channel system 3 can be designed as depressions of different depths.
  • the chamber 2 or the channel system 3 is fluidically connected to the outside world via a fluidic interface 5 .
  • the fluidic interface 5 is an opening in the channel system on a side surface of the structured component 1.
  • the opening in the fluidic interface 5 can also be arranged on an upper side or underside of the fluidic system.
  • the structured interface 5 can protrude from a side surface of the structured component 1 as a projection.
  • the fluidic system can have multiple fluidic interfaces 5 which are each connected to the channel system 3 .
  • the fluidic interfaces 5 can be at different Be arranged surfaces of the structured component 1, such as the top, bottom, or side surfaces.
  • the openings of the fluidic interfaces 5 can point in different directions, ie they can have different orientations with respect to the center point of the structured component 1 .
  • a second component 4 closes the channel system 3 and the chamber 2 in a liquid-tight and gas-tight manner, so that liquids and gases can only be supplied and discharged via the fluidic interface 5 .
  • the second component 4 is arranged on the surface of the structured component 1 in such a way that it closes the chamber 2 and the channel system 3 on the upper side of the structured component 1 .
  • the second component 4 can, for example, be glued onto the structured component 1 or welded to the structured component.
  • the interior of the chamber 2 is delimited by the underside of the second component 4 on the upper side of the chamber 2 .
  • the chamber 2 can have a substantially flat oval, rectangular or round shape.
  • the chamber 2 or the interior of the chamber 2 is thus defined on the one hand by the structured component 1 and on the other hand by the second component 4 .
  • the second component 4 is flexible or the second component has a flexible or movable area 6 .
  • the flexible area 6 of the second component 4 is arranged above the chamber 2 as a direct part of the second component 4 .
  • the flexible or movable area 6 can be designed as a further component of the fluidic system.
  • the flexible or moveable area 6 of the second component 4 should be arranged at least on an area of the chamber 2 or on the outside of the chamber 2 .
  • the second component 4 can be formed, for example, as a film or strip and can be made of plastic or metal.
  • the structured component 1 has a flexible area 7 below the chamber.
  • the flexible area 7 is arranged between the chamber floor and the underside of the structured component 1 .
  • the flexible area 7 can either be implemented by applying a further component to the structured component 1 or directly via the material properties of the structured component 1 itself or by manufacturing it from more than one material, for example by multi-component injection molding.
  • the structured component 1 is with the second component 4 and beyond closed with another component 8, one or both components 4 and 8 can have a flexible or movable area.
  • the second component 4 is arranged on the upper side of the structured component 1 . That is, the top of the chamber 2 is closed with the second component 4 .
  • the further component 8 is arranged on the underside of the structured component 1 . That is, the underside of the chamber, ie the chamber floor, is closed with the additional component 8 .
  • a flexible area 9) is shown in the further component 8.
  • the structured component 1 is preferably formed with a cover film that has sufficient flexibility to be pressed in and lifted above or below the chamber 2 .
  • the chamber 2 is preferably designed in such a way that the flexible area(s) 6 , 7 , 9 does not fill the entire chamber 2 when it is pressed into the chamber 2 .
  • the flexible area 6, 7, 9 when the flexible area 6, 7, 9 is pushed into the chamber 2, the flexible area does not close flush with the bottom of the chamber. That is, liquid or gas located in the chamber 2 is not completely displaced from the chamber 2 by the flexible region 6, 7, 9 being pushed in.
  • a tight seal of the flexible areas 6, 7, 9 with the chamber floor or adjoining channel systems 3 is not necessary for the functionality.
  • Taking up liquid In order to take up liquids/gases into the fluidic system, more precisely into the chamber 2 of the fluidic system, the flexible area 6 is moved manually, eg with a finger of a user, or by means of an operating device from the starting position depressed. In other words, the flexible portion 6 is pressed into the chamber 2 from its initial position by pressure. That is, the flexible area 6 is pressed into the interior of the chamber 2 via the upper side. By pressing the flexible area 6 into the chamber 2, the interior space of the chamber 2 is reduced. The fluidic interface 5 is then immersed in a liquid.
  • the flexible area 6 moves either automatically, due to the material properties of the flexible area 6, partly or completely back into the starting position, or is moved back into the starting position by a movement of the operating device, eg suction or lifting.
  • the interior space of the chamber is increased again by moving the flexible area 6 back into the starting position.
  • the increase in volume of the interior creates a negative pressure in the chamber 2 or in the adjacent channel system 3, which is connected to the liquid via the fluidic interface. That is, liquid is drawn into the fluidic system by the negative pressure.
  • the negative pressure is first drawn into the channel system 3 and, if the negative pressure is sufficiently large, then also into the chamber 2 under negative pressure. Liquid is thus absorbed into the fluidic system.
  • the volume of the interior of the chamber 2 displaced by pressing down the flexible area 6 and/or by returning the flexible area 6 to the starting position in a defined manner, the volume of the liquid taken up or the positioning of the liquid in the channel system 3 or in of chamber 2 of the fluidic system.
  • the liquid taken up is mixed by first drawing liquid into chamber 2, i.e. liquid is first taken up into the fluidic system. Then either the flexible component 6 is moved or the fluidic system itself is moved. The fluidic system is moved, for example, by tilting the fluidic system multiple times. Rapid shaking should be avoided to prevent air bubbles from forming in the absorbed liquid.
  • the delivery of liquids from the fluidic system takes place in that the flexible component 6 or the flexible components are pressed into the chamber 2 .
  • the volume of the interior space of the chamber 2, which is delimited by the flexible component is reduced by the flexible component being pressed in.
  • the liquid, which is located either in the chamber 2 or in the channel system 3, is discharged from the fluidic system according to the volume displaced by the movement of the flexible area 6, ie by the flexible area 6 being pressed into the chamber 2. That is, the displaced liquid is released from the chamber 2 via the channel system 3 through the fluid interface 5 .
  • the volume of the liquid dispensed may correspond to the volume of the interior of the chamber 2 by which the chamber is reduced by pushing in the flexible portion.
  • volumes of liquid can be dispensed multiple times.
  • the multiple delivery can take place in that the flexible area 6 , 7 , 9 is gradually pressed further and further into the chamber 2 or the interior of the chamber 2 .
  • the multiple delivery can also take place in that the flexible area 6, 7, 9 is first pressed into the chamber 2 and that the flexible area 6, 7, 9 then moves out of the chamber 2 either independently as described above or with the help of a Operating device is moved out of the chamber 2.
  • the outward movement is accompanied by a backflow of at least part of the liquid in the channel system 3 connected to the chamber 2 .
  • the withdrawal is followed by a repeated depression of the flexible portion 6, 7, 9 into the chamber 2 for renewed liquid delivery.
  • a pumping movement or a pumping functionality is carried out. This leads to repeated and alternating liquid intake and liquid discharge.
  • Closure of the fluidic interface 5 for sampling The fluidic interface 5 for sampling is closed by a cap 14 . Due to the design of this cap 14, the volume in the channel system 3 can also be displaced by integrated projections.
  • One fluidic interface 5 is preferably configured as an input 5.1 and another fluidic interface 5 is configured as an output 5.2 of the fluidic system.
  • the entrance 5.1. and output 5.2 are preferably formed on the structured component 1.
  • the two fluidic interfaces 51 and 5.2 are formed on one side, preferably on an end face or narrow side of the chip (fluidic system). This means that the input and the output are arranged on one side of the system. This makes it possible to close the input and output with a cap 14, which is also referred to as a jumper.
  • the cap 14 is preferably attached to the fluidic system, preferably to the structured component 1 .
  • One or more caps 14 may be attached.
  • only one cap 14 is provided, which can be plugged onto either the input 5.1 or the output 5.2. A liquid can then be selectively taken up at the inlet or liquid can be dispensed at the outlet.
  • the one or more caps 14 are attached to the chip via a tab 44 .
  • the flexible region 6 can thus be pressed under a plane defined by the upper side of the structured component 1 into the chamber 2, more precisely into the interior of the chamber 2, by external pressure.
  • the flexible area 6 can be pulled out of the interior of the chamber 2 again by pulling from the outside, e.g. That is, it can be moved beyond the plane defined by the top of the structured component 1.
  • the design of the fluidic system according to the above-described embodiments means that the fluidic system can be used as a pipette with functions of liquid intake, liquid delivery and multiple intake and delivery of liquids due to the design of the chamber 2 and the flexible areas 6, 7, 9.
  • the operation can be carried out completely manually without additional tools or by means of an operating device.
  • FIGS. 4a to 4c show embodiments of the fluidic interface 5.
  • the embodiments of the fluidic interface 5 according to FIGS. 4a to 4c differ in their geometry. More precisely, the shown embodiments of the fluidic interface 5 each have an outlet 10, the shape of the outlet 10 being different in the shown embodiments.
  • the special or defined geometry of the outlet and/or a surface modification or a material composition of the outlet 10 of the fluidic interface can be used to set the volume of a droplet of the liquid dispensed at which the droplet breaks away from the outlet. Due to the defined geometry of the outlet 10 of the fluidic interface 5, volumes, i.e. desired volumes, of the liquid drop of the liquid dispensed can be preset.
  • the geometry of the outlet 10 of the fluidic interface 5 is also decisive for the volume of liquid dispensed.
  • the flexible area 6 , 7 , 9 is pressed into the chamber 2 so that a drop of liquid forms at the outlet 10 of the fluidic interface 5 .
  • the flexible area 6 , 7 , 9 is pressed further into the chamber 2 until the drop of liquid tears away from the outlet 10 .
  • the pressing in of the flexible area 6, 7, 9 or the dispensing of liquid can then be terminated.
  • the flexible portion 6, 7, 9 can be pushed further into the chamber 2 to create another drop of liquid.
  • Figures 5f to 5f 12 show pressure elements of the flexible regions according to various embodiments.
  • the flexible areas 6, 7, 9 can have pressure elements 11, 12, 13 in order to ensure that the flexible areas 6, 7, 9 are pressed into the chamber 2 in a defined manner or that the flexible areas 6, 7, 9 are pulled out or moved out in a defined manner to allow the chamber 2.
  • pressure elements 11, 12, 13 can be arranged or applied to the flexible areas 6, 7, 9.
  • the pressure elements 11, 12, 13 can ensure that the same volume of the interior of the chamber 2 is always displaced when the flexible region 6, 7, 9 is pressed into the chamber 2.
  • the pressure elements 11, 12, 13 can be operated either manually, for example with a finger, or by an operating device.
  • the Printing elements 11, 12, 13 can be materials applied to the flexible area 6.
  • the pressure elements 11 can be designed as a silicon hemisphere, such as in Figures 5a and 5b shown.
  • the pressure elements 12 can be manufactured directly with the flexible area 8, for example by multi-component injection molding, as in Figures 5b and 5c shown.
  • a defined impression can also be achieved using pressure elements 13, which are protruding elements in the structured component, as in Figures 5e and 5f shown.
  • Figures 5e and 5f The pressure elements 13 shown are arranged in the chamber 2 of the fluidic system, for example on the chamber floor, and protrude into the interior of the chamber 2 .
  • the movement of the flexible area 6 when it is pressed into the chamber 2 can thus be limited by means of the pressure elements 13, so that only a maximum volume of the interior space is displaced.
  • Figures 5a, 5b, and 5e each show the initial state of the flexible area 6, 7, 9, ie the state when no force or pressure is exerted on the flexible area 6, 7, 9.
  • Figures 5b, 5d and 5f each show a position before liquid is taken up or during liquid discharge, ie a position of the flexible region 6, 7, 9 when it is pressed into the chamber 2.
  • Figures 6a and 6b show further embodiments of the fluidic system. More precisely show Figures 6a and 6b a fluidic system which has two separate fluidic interfaces 5 .
  • the fluidic interfaces 5 are arranged on different, more precisely opposite, side surfaces of the structured component 1 and protrude from the respective side surfaces.
  • liquid can be taken up through one of the two fluidic interfaces 5 and liquid can be removed through the other of the two fluidic interfaces 5 .
  • the fluidic interfaces 5 can also be closed by a cap 14 in order to prevent contamination or liquid from escaping from the fluidic interface 5 .
  • the liquid contained in the fluidic system can be transported and stored in a particularly safe and simple manner through the cap 14 .
  • the cap 14 can be placed on the fluidic interface 5, more precisely, on the opening formed by the fluidic interface 5 in a side surface of the structured component, and the fluidic interface 5 can be closed off in a fluid-tight manner.
  • the fluidic system can be expanded by a liquid reservoir 16 .
  • the liquid reservoir 16 is connected to the channel system 3 or to the chamber 2 via a channel.
  • the channel can be part of the channel system 3.
  • the liquid reservoirs 16 can be formed, for example, by one or more so-called blisters, ie liquid-filled compartments which can be opened, for example by piercing, and which are mounted on the fluidic system in a liquid-tight manner.
  • a liquid intake from the blister is achieved by depressing the flexible portion 6 as described above and moving the flexible portion 6 out of the chamber 2, with the resulting negative pressure in the Chamber 2 and the channel system 3 is received via the connected channel liquid from the blister in the channel system 3 and the chamber 2.
  • a cap 14 is placed on the fluidic interface to prevent liquid from escaping from the fluidic interface 5 if further liquid is pushed into the chamber 2 by emptying the liquid reservoir 16 in the channel system 3 and the liquid from the liquid reservoir 16 is also pushed in chamber 2 is flowing.
  • liquid that is received into the fluidic system from the outside and is located in the channel system 3 or in the chamber can be mixed with the liquid in the liquid reservoir 16 .
  • the mixing can be facilitated or intensified by placing the cap 14 on the fluidic interface, since with the cap 14 in place the negative pressure resulting from the movement of the flexible area 6 acts on the liquid in the liquid reservoir 16 .
  • the liquid reservoir 16 may also be referred to as a reagent reservoir or liquid reagent reservoir and may contain any type of liquid.
  • the liquids can be mixed by a movement of the fluidic system, a movement of the flexible area 6, 7, 9 or introduced mixing elements.
  • the mixing elements e.g. silicone balls, can be created by manually moving the fluidic system.
  • the mixing can be done by means of elements made of magnetic materials, which are moved by an external mixing device.
  • Figures 7a and 7b show an embodiment of the fluidic system that combines two types of liquid intake.
  • the sample is taken up, for example, through the fluidic interface 5 serving as a liquid inlet, by moving the flexible area 6, 7, 8 of the chamber 2 into the chamber 2 and moving the flexible area out, as described above.
  • liquid can be taken up automatically into the fluidic system via passive filling, ie by means of capillary forces of the channel system 3 at the fluidic interface 5 .
  • the suction effect which is caused by the negative pressure or by the capillary forces, and thus the filling speed, can be intensified or accelerated by a surface modification, for example hydrophilicization of the channel surface of the channel system 3.
  • the channel system 3 by means of passive valves in the channel system 3, for example capillary stop valves and channel constrictions 41, see Figure 7a , the channel system 3, the volume of the recorded liquid can be determined. A defined quantity of liquid is thus taken up, with a closure cap preventing the liquid from escaping when the liquid reservoir 16 is emptied.
  • Figures 8a to 8e 12 show a liquid reservoir 16 ejection mechanism according to embodiments not falling under the scope of the invention.
  • the squeezing mechanism can be designed, for example, as a flap 19, with the latching of the flap 19, as in Figure 8d shown the introduction of a defined amount of liquid from the liquid reservoir 16 into the channel system 3 of the fluidic system, whereby a defined mixing ratio of the liquid from the liquid reservoir with the liquid contained in the fluidic system is achieved.
  • Figure 8d shows a state in which the flap 19 presses the liquid reservoir 16 onto the fluidic interface 5 of the channel of the channel system 3. This principle can be extended to other liquid reservoirs 16 and can therefore be used for multiple mixtures.
  • Figure 8a shows an ejection mechanism with a seat 17, which can be designed as a blister seat and has piercing elements 18, for example small tips.
  • Figure 8b 14 shows an embodiment of an ejection mechanism, wherein the seat 17 has detents 19 and the flap 19 is movably mounted on the detents 20 of the seat 17 in a hinge-like manner.
  • the liquid reservoir 16 is located on the flap.
  • the push-out mechanism shown can also have push-through elements 18 (not shown).
  • One of the latching lugs 20 serves as a hinge and another of the latching lugs 20 serves as a latching surface or bearing surface for the cap 19 in order to limit rotation of the cap 19 in this way.
  • a defined or predetermined amount of liquid can be released from the liquid reservoir to the fluidic system.
  • the seat 17 can also be referred to as the reservoir interface.
  • FIG. 1 shows an embodiment of the ejection mechanism in which the liquid reservoir 16 is arranged on the surface of the structured component 1.
  • the flap 19 can have a bulge or projection, as in FIG Figure 8d shown, so that the liquid reservoir 16 is expressed by the projection when the flap 19 is closed.
  • Figure 8d shows the depressed ejection mechanism, in this case the flap 19..
  • Figure 8e 14 is a plan view of a seated ejector mechanism 17 according to one embodiment.
  • Figures 9a and 9b show a fluidic system with a long channel system 3.
  • the channel system 3 meanders between the fluidic interface 5 and the chamber 2, whereby the length of the channel system 3 is increased. This creates a dwell stretch created for the liquid contained in the fluidic system.
  • the dwell zone can be filled with reagents, for example dried reagents.
  • a long channel system 3 can be formed.
  • the channel system 3 can also have widenings 22 for better mixing, as in Figure 9a shown, or have a further passive mixing element. As shown, the expansions can be elongate or in the direction of flow in the channel system 3 .
  • Liquid or reagents can be introduced into the expansions 22, which are mixed with the liquid taken up into the channel system 3 or the fluidic system or mixed with the liquid discharged from the fluidic system.
  • the channel system 3 can also have an optical detection chamber or reaction chamber 22, 21, as in Figure 9b shown.
  • a configuration of the detection chamber 21 at different depths is particularly advantageous in order to expand the dynamic range of the measurement.
  • the detection chamber 21 can be let into the structured component 1 at different depths, so that it has, for example, detection chamber floors of different depths in steps.
  • FIG. 10a to 10c Another option for expanding the chamber functionality is the introduction of a lateral flow strip 23, as in Figures 10a to 10c shown, which can be filled in a defined manner by means of the pumping function of the fluidic system.
  • a combination of filling by the pumping effect of the chamber 2 in the manual operation described above or by means of an operating device and the suction effect of the lateral flow strip can also take place.
  • the lateral flow strip is embedded or introduced into a further chamber, which is also connected to the channel system 3 .
  • the use of ventilation channels 25 or gas-permeable and liquid-tight membranes 24, which are respectively connected to the channel system 3 or the chamber of the lateral flow strip, for operating the system is particularly advantageous. This is the case, for example, for the gas-permeable and liquid-tight membranes 24 in Figure 10b and for vent ducts 25 in Figure 10c shown.
  • 11 12 shows a fluidic system according to yet another embodiment.
  • the structured component 1 has two chambers 2, which are embedded in the upper side of the structured component.
  • the two chambers 2 are directly connected to one another via a first channel system 3 or a channel.
  • the two chambers 2 are also each connected to the environment via a fluidic interface 5 via a respective second channel system 3 or channel.
  • This configuration of the fluidic system can also be referred to as a chamber system combined with one another.
  • the use of chamber systems combined with one another, which can then be used simultaneously as a mixing, reaction, pumping and/or dosing unit, is a further embodiment of the fluidic system.
  • Figures 12a to 12d show embodiments of the fluidic system with distribution systems 26.
  • a chamber 2 is connected to a manifold system 26 at one end.
  • the distribution system 26 can be part of the duct system 3 .
  • the distribution system 26 has one or more channels leading away from the chamber 2 and branching out. The ends of the respective branched channels of the distribution system 26 are each connected to a fluidic interface 5 .
  • one channel leads away from the chamber 2 and branches into four channels each, which are each connected to a fluidic interface.
  • Figures 12a and 12b show a fluidic system with a distribution system 26, the channel leading away from the chamber 2 branching off step by step, namely initially into two further channels. The two further channels then each branch into two further channels, so that the channel leading away from the chamber 2 branches into a total of four channels, which open into the respective fluidic interfaces 5 .
  • all fluidic interfaces 5 are controlled or actuated simultaneously by a movement of the flexible area 6, 7, 9.
  • the branched channels of the distribution system 26 can have diaphragm valves 27 .
  • diaphragm valves 27 require the diaphragm valves 27 to be pressed in and sealed in a liquid-tight manner in order to close the respective channels individually or together and thus to be able to take in or release liquid via the fluidic interfaces 5 .
  • the liquid flow within the respective channels can be controlled in a targeted and defined manner by means of the membrane valves 27 . That is, the individual fluidic interfaces 5 can be specifically controlled or actuated by means of the membrane valves 27 . That is, they can be controlled independently of one another.
  • the diaphragm valves 27 can be or can be brought into a state that does not allow liquid flow in the respective channel, a state that allows an unimpeded liquid flow in the respective channel and/or a state that allows a reduced liquid flow in the respective channel being controlled. In this way, a defined and/or simultaneous liquid intake or liquid discharge can be controlled in a targeted manner via the respective fluidic interfaces 5 .
  • Figures 12c and 12d show an embodiment of the fluidic system with a distribution system 26, in which the channel leading away from the chamber 2 branches at one point in a star shape into four further channels.
  • a rotary valve 28 can be arranged at the point of branching, which is operable from the outside manually or by means of a device. With the help of the rotary valve 28, a targeted flow of liquid can thus be connected between the channel leading away from the chamber 2 and one or more channels with the branched channels, ie channels connected to the fluidic interfaces 5.
  • the body of the rotary valve 28 may itself have one or more recessed channels 29 which, when appropriately positioned at the point of bifurcation, form the seat 28a of the rotary valve 28 can connect the branched or connected channels with each other.
  • a distribution channel 29 integrated in the rotary valve body (28b) the option with a rotary valve 28 allows sequential or parallel fluid intake or discharge via one or more fluidic interfaces 5, which in turn is controlled by changing the chamber volume.
  • the structured component 1 has a flexible area 7 below the chamber 2, which is realized either by applying another component in the structured component 1 or directly via the material properties of the structured component 1 itself or by manufacturing it from more than one material eg is implemented by multi-component injection molding.
  • Figures 14a and 14b shown as a top view or as a sectional view, with an enlargement function 42 being introduced into the structured component 1 at a defined position above or below the chamber 2 or the channel system 3, which is designed, for example, in the form of a lens, in order to reach certain positions in the channel system 3 to be able to track better through the liquid and also to be able to read color reactions better as indicator reactions.
  • FIG. 15a to 15c Another embodiment is in Figures 15a to 15c shown, with longer channel elements being introduced as flow limiters 43 into the course of the fluid in the channel system 3 in order to enable controlled liquid intake and discharge.
  • the flow restrictors have a meandering shape and/or can be designed as channel tapers in order to control the flow of a fluid and/or to limit the speed
  • the chamber 2 can be connected to a plurality of channels or channel systems 3, which each open into at least one fluidic interface 5.
  • the fluidic system can therefore have a plurality of fluidic interfaces 5 and the chamber 2 can have a plurality of channels or channel systems 3 branching off from them.
  • FIG. 16 shows an embodiment of the chip in a view from above.
  • the structured component 1 with a chamber 2 and the channel system 3 is shown.
  • the channel system 3 connects the input 5.1. with chamber 2 and connects the chamber to the outlet 5.2.
  • a flow restrictor 43 is integrated into the channel system 3, which is designed in a meandering manner and/or can contain narrowing of the channel, with which the flow rate of the fluid can be controlled or reduced.
  • a reservoir interface 17 with a liquid reservoir 16 is connected to the channel system 3 .
  • the input and output can be closed with a cap 14 which is attached to the chip by means of a tab 44 .
  • a cap 14 which can be plugged alternately onto the input or output, in order to enable the chip to selectively absorb fluids when the input is open, ie without a cap 14, and the output is 5.2. is closed with the cap 14.
  • a necessary negative pressure can be built up in order to take in a fluid via the fluidic interface 5.1 (input).
  • the fluid should be released again.
  • the cap 14 is then placed on the input and this is closed in a fluid-tight manner. Then the fluid via the output 5.2. be given. A switching between two functions of the chip can thus be made possible via the cap 14 .
  • caps 14 it is possible to attach several caps 14 to the chip, for example to enable the chip to be transported or stored, with either the inside of the chip being protected from dirt and/or fluids present inside being able to leak out is prevented.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)

Claims (15)

  1. Système microfluidique comprenant :
    - un composant structuré (1) réalisé de manière plate, ayant une chambre (2) et un système de canal (3),
    - au moins une interface fluidique (5) par l'intermédiaire de laquelle le système de canal (3) et la chambre (2) sont fluidiquement reliés à l'extérieur,
    caractérisé par :
    - un composant (4) agencé sur une surface du composant structuré (1) de telle sorte qu'il ferme la chambre (2) et le système de canal (3) de manière étanche aux fluides sur la surface du composant structuré (1),
    - dans lequel le composant (4) et/ou le composant structuré (1) comportent une zone souple ou mobile (6), accessible depuis l'extérieur, qui est au moins partiellement adjacente à la chambre (2),
    dans lequel la zone souple ou mobile (6) est conçue pour être poussée dans la chambre (2) ou être déplacée hors de la chambre (2) à la main ou avec un dispositif d'actionnement, de telle sorte que des liquides ou des gaz peuvent être admis ou évacués par l'intermédiaire de la au moins une interface fluidique (5) ou peuvent être mis en mouvement dans le système microfluidique, et
    dans lequel l'interface fluidique (5) fait saillie à partir d'une face latérale du composant structuré (1) sous la forme d'une saillie, afin d'ajuster un volume prédéfini d'une goutte de liquide à évacuer au moyen d'une géométrie de sortie de la saillie.
  2. Système microfluidique selon la revendication 1, dans lequel la chambre (2) est reliée à une autre interface fluidique (5) par l'intermédiaire d'un autre système de canal (3) et au moins une des interfaces fluidiques (5) peut être fermée avec un capuchon (14) du système microfluidique et/ou dans lequel un canal (3) menant à la chambre (2) comporte des élargissements (22) et/ou dans lequel des éléments de canal plus longs sont introduits en tant que limiteurs de débit (43) dans le trajet de fluide du système de canal (3), afin de permettre une admission et une évacuation contrôlées du liquide et/ou un mouvement défini de la zone souple (6, 7, 9) est permis au moyen d'éléments géométriques ou de supports (11, 12, 13).
  3. Système microfluidique selon la revendication 2, dans lequel le capuchon (14) comporte une zone souple conçue pour être poussée ou tirée après la mise en place, pour mettre ainsi en mouvement le liquide se trouvant dans le système de canal (3), et/ou dans lequel une interface fluidique (5) est formée comme une entrée (5.1) et une interface fluidique (5) est formée comme une sortie (5.2) du système fluidique, et l'entrée et la sortie sont agencées sur un côté du système, dans lequel un capuchon (14) est fixé sur le système fluidique, de préférence sur le composant structuré (1), lequel capuchon peut être monté sur l'entrée (5.1) ou la sortie (5.2) en permettant ainsi une admission d'un liquide au niveau de l'entrée (5.1) ou une évacuation de liquide au niveau de la sortie (5.2).
  4. Système microfluidique selon l'une des revendications 1 à 3, contenant en outre un dispositif d'évent pour la chambre (2), dans lequel le dispositif d'évent est agencé de telle sorte qu'un évent peut avoir lieu par l'intermédiaire d'un canal supplémentaire (25) du système microfluidique relié à l'extérieur ou d'une membrane perméable au gaz (24) du système microfluidique, et/ou dans lequel le dispositif d'évent peut être fermé.
  5. Système microfluidique selon l'une des revendications 1 à 4, contenant en outre un réservoir de réactifs supplémentaire (16) qui est configuré sous la forme d'une alvéole et/ou dans lequel la chambre (2) dispose de réactifs préalablement introduits.
  6. Système microfluidique selon la revendication 5, dans lequel le réservoir de réactifs (16) comprend :
    - un logement d'alvéole (17) comportant des éléments pointus (18) formés de manière à percer l'alvéole (16) reliée de manière étanche aux liquides au-dessus desdits éléments,
    - un clapet (19) qui peut être poussé par des éléments de guidage (20) dans le logement d'alvéole (17), en permettant ainsi le dosage d'un volume défini, dans lequel une configuration particulière des éléments de guidage (20) permet de préférence un dosage de volume en plusieurs étapes.
  7. Système microfluidique selon la revendication 6, dans lequel le clapet (19) permet une pression définie de volumes définis et/ou le clapet (19) et des éléments géométriques ou des supports (11, 12) formés comme des éléments de pression sont reliés, combinés ou couplés les uns aux autres sur la zone souple ou mobile (6, 7 9).
  8. Système microfluidique selon l'une des revendications 1 à 7, comportant une cavité (21) pour la lecture optique et/ou la réaction, ayant de préférence différentes profondeurs et/ou dans lequel le système microfluidique comporte une bande d'écoulement latéral (23) dont le remplissage est permis par un actionnement de la chambre, dans lequel une membrane d'évent (24) et/ou un canal d'évent (25) est de préférence couplé à la bande d'écoulement latéral (23).
  9. Système microfluidique selon l'une des revendications 1 à 8, ayant au moins deux chambres (2), dans lequel les au moins deux chambres (2) sont directement reliées l'une à l'autre par l'intermédiaire d'un système de canal (3), dans lequel les au moins deux chambres (2) sont agencées dans un ou plusieurs plans et/ou des supports (11, 12, 13) sont agencés sur le composant souple ou mobile (6), lesquels supports se situent à l'extérieur de la chambre (2) ou s'étendent dans la chambre (2).
  10. Système microfluidique selon l'une des revendications 1 à 9, comportant en outre des éléments mobiles insérés dans la chambre (2) en vue d'un mélange, lesquels éléments sont de préférence réalisés sous forme de billes ou de tiges et/ou des éléments structurels sont formés dans le composant structuré (1) afin d'améliorer le mélange et/ou un dispositif de mélange.
  11. Système microfluidique selon l'une des revendications 1 à 10, dans lequel le système de canal (3) comporte des repères d'ajustement ou des repères d'ajustement sont marqués à côté, en dessous ou au-dessus du système de canal (3) pour permettre une indication de volume.
  12. Système microfluidique selon l'une des revendications 1 à 11, comprenant au moins un élément parmi :
    - une soupape rotative (28) par l'intermédiaire de laquelle une admission ou une évacuation de fluides peut être commandée,
    - une soupape à diaphragme (27) par l'intermédiaire de laquelle une admission ou une évacuation de fluides peut être commandée,
    - des soupapes dans le système de canal (3) pour l'admission de volumes définis de liquide, dans lequel une fonction de soupape peut être générée ou améliorée par une fonctionnalisation de surface, dans lequel les soupapes (27, 28) permettent une admission de liquide sélective à partir d'interfaces fluidiques individuelles (5),
    - un système de distribution (26) relié à plusieurs interfaces fluidiques (5), dans lequel les interfaces fluidiques (5) peuvent être commandées de manière sélective et/ou un système de distribution (26) comprenant une pluralité de canaux, qui débouchent dans un nombre correspondant d'interfaces fluidiques (5) et permettent l'admission et l'évacuation simultanées de liquides et/ou une distribution uniforme de liquides dans le système de distribution (26) est assurée par des soupapes passives intégrées (41).
  13. Système microfluidique selon l'une des revendications 1 à 12, comportant en outre une interface de réservoir (17), au moyen de laquelle un réservoir de liquide (16) peut être relié au composant structuré (1), dans lequel l'interface de réservoir (17) est de préférence fluidiquement reliée au système de canal (3) et/ou à la chambre (2).
  14. Système microfluidique selon l'une des revendications 1 à 13, dans lequel des réactifs secs sont incorporés dans le système de canal (3) du composant structuré (1), dans lequel les réactifs secs sont absorbés par les fluides qui s'écoulent et sont mélangés avec ceux-ci ou un réactif est placé à une position définie dans ou sur le système de canal (3) afin de colorer le liquide qui s'écoule sur celui-ci, de sorte que l'atteinte de la position et ainsi l'obtention d'un volume prédéfini ou d'un temps de séjour défini est indiquée.
  15. Système microfluidique selon l'une des revendications 1 à 14, dans lequel un dispositif de grossissement, réalisé sous la forme d'une lentille, est agencé à au moins une position définie au-dessous ou en dessous du système de canal (3) ou de la chambre (2), de telle sorte que l'atteinte d'au moins une position déterminée dans le système de canal (3) peut être détectée par le liquide et/ou des réactions de coloration.
EP17808819.1A 2016-11-16 2017-11-16 Dispositif de prélèvement, d'expulsion et de déplacement de liquides Active EP3541516B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102016122056.6A DE102016122056B4 (de) 2016-11-16 2016-11-16 Mikrofluidisches System zur Aufnahme, Abgabe und Bewegung von Fluiden
PCT/EP2017/079510 WO2018091608A1 (fr) 2016-11-16 2017-11-16 Dispositif de prélèvement, d'expulsion et de déplacement de liquides

Publications (3)

Publication Number Publication Date
EP3541516A1 EP3541516A1 (fr) 2019-09-25
EP3541516B1 true EP3541516B1 (fr) 2023-08-23
EP3541516C0 EP3541516C0 (fr) 2023-08-23

Family

ID=60574548

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17808819.1A Active EP3541516B1 (fr) 2016-11-16 2017-11-16 Dispositif de prélèvement, d'expulsion et de déplacement de liquides

Country Status (7)

Country Link
US (1) US11446657B2 (fr)
EP (1) EP3541516B1 (fr)
KR (2) KR20190085043A (fr)
CN (1) CN110177620A (fr)
DE (1) DE102016122056B4 (fr)
ES (1) ES2959736T3 (fr)
WO (1) WO2018091608A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102018111822B4 (de) * 2018-05-16 2021-10-07 Microfluidic Chipshop Gmbh Fluidisches System zur Aufnahme, Abgabe und Bewegung von Flüssigkeiten, Verfahren zur Verarbeitung von Fluiden in einem fluidischen System
KR102272251B1 (ko) * 2019-05-02 2021-07-02 재단법인 오송첨단의료산업진흥재단 다중 분석을 위한 분기형 바이오 칩
GB2588760B (en) * 2019-10-30 2022-06-22 Thermo Fisher Scient Bremen Gmbh Interface system and corresponding method
WO2021164021A1 (fr) * 2020-02-21 2021-08-26 Boe Technology Group Co., Ltd. Structure microfluidique, système microfluidique, procédé microfluidique et procédé de fabrication de structure microfluidique
EP4129480A1 (fr) * 2021-08-06 2023-02-08 Microliquid SL Système d'ouverture de blister comprenant un blister et un poussoir d'actionnement
CN117916023A (zh) * 2021-09-14 2024-04-19 伊鲁米纳公司 井凹组件和相关方法
GB2616016A (en) 2022-02-23 2023-08-30 Mint Diagnostics Ltd Integrated microfluidic test strip

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19802368C1 (de) * 1998-01-22 1999-08-05 Hahn Schickard Ges Mikrodosiervorrichtung
BR0109432A (pt) 2000-03-21 2004-06-22 Univ Illinois Nariz artificial tendo um arranjo de corantes e método para olfação artificial
EP1205247A2 (fr) * 2000-10-16 2002-05-15 Ngk Insulators, Ltd. Micro-pipette, dispositif distributeur et méthode pour la production de bio-puces
GB0110476D0 (en) * 2001-04-30 2001-06-20 Secr Defence Reagent delivery system
DE10202996A1 (de) * 2002-01-26 2003-08-14 Eppendorf Ag Piezoelektrisch steuerbare Mikrofluidaktorik
DE102005052752A1 (de) * 2005-11-04 2007-05-10 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
WO2007057744A2 (fr) * 2005-11-15 2007-05-24 Inverness Medical Switzerland Gmbh Reference croisee a des demandes connexes
WO2008143902A2 (fr) * 2007-05-14 2008-11-27 American Bio Medica Corporation Dispositif de manipulation de fluide ou pipette comprenant une poire à surface aplatie
JP6110067B2 (ja) * 2009-02-06 2017-04-05 ノースウェスタン ユニバーシティ 破裂可能な液体パッケージングとその使用
DE102009032744A1 (de) 2009-07-11 2011-01-13 Thinxxs Microtechnology Ag Fluidspeicher
EP3046663A4 (fr) 2013-09-18 2017-05-31 California Institute of Technology Système et procédé de régulation de mouvement et de minutage
WO2015077412A1 (fr) 2013-11-22 2015-05-28 Rheonix, Inc. Pompe sans canal, ses procédés et ses applications
CN204093469U (zh) * 2014-09-25 2015-01-14 浙江硕华医用塑料有限公司 一次性扁平移液吸头
CN204514801U (zh) 2015-04-01 2015-07-29 杭州霆科生物科技有限公司 一种用于农药残留现场检测的可抛型微流控芯片

Also Published As

Publication number Publication date
CN110177620A (zh) 2019-08-27
KR20190085043A (ko) 2019-07-17
DE102016122056A1 (de) 2018-05-17
EP3541516C0 (fr) 2023-08-23
DE102016122056B4 (de) 2021-02-18
EP3541516A1 (fr) 2019-09-25
WO2018091608A1 (fr) 2018-05-24
KR102450612B1 (ko) 2022-10-06
ES2959736T3 (es) 2024-02-28
KR20210135648A (ko) 2021-11-15
US20200038862A1 (en) 2020-02-06
US11446657B2 (en) 2022-09-20

Similar Documents

Publication Publication Date Title
EP3541516B1 (fr) Dispositif de prélèvement, d'expulsion et de déplacement de liquides
EP2437890A1 (fr) Dispositif pour transporter un fluide dans un canal d'élément microfluidique
EP1426108B1 (fr) Dispositifs de dosages paralleles pour liquides
US20040208794A1 (en) Microfluidic mixing and dispensing
DE10022398A1 (de) Gaspolster-Mikrodosiersystem
EP3393661B1 (fr) Dispositif microfluidique et procédé de fonctionnement d'un dispositif microfluidique
DE102009048378B3 (de) Mikrofluidische Struktur
EP3793736A1 (fr) Système fluidique pour recevoir, délivrer et déplacer des fluides, procédé de traitement de fluides dans un système fluidique
EP2988871B1 (fr) Procédé de remplissage d'un dispositif microfluidique par un système de distribution et système d'essai correspondant
EP1256543A2 (fr) Dispositif microfluidique de réservoir et/ou de dosage
WO2018001647A1 (fr) Cuve à circulation dotée d'une zone de stockage de réactif
EP2905079A1 (fr) Dispositif de stockage préalable d'un fluide dans un système micro-fluidique, procédé de fonctionnement et procédé de fabrication d'un tel dispositif
DE102010047384B4 (de) Vorrichtung und Verfahren zur Erzeugung oder zur Ablage eines Fluidstroms aus Fluidsegmenten und ihre Verwendung
EP0187167B1 (fr) Appareil doseur pour liquides
DE10102152C1 (de) Vorrichtung und Verfahren zum Dosieren kleiner Flüssigkeitsmengen
DE102016015944B3 (de) Mikrofluidisches System zur Aufnahme, Abgabe und Bewegung von Fluiden
DE202014104510U1 (de) Vorrichtung zum Vorlagern eines Fluids in einem mikrofluidischen System
EP3260822B1 (fr) Seringue multivoie pour l'utilisation a l'aide d'un dispositif de dosage
EP3649471B1 (fr) Module d'analyse de fluide et analyseur de fluide
EP3182136A1 (fr) Sachet en film pour un système d'analyse microfluidique, système d'analyse microfluidique, procédé de fabrication et procédé de fonctionnement d'un système d'analyse microfluidique
DE2559090A1 (de) Behaelter zum aufbewahren und tropfenweisen abgeben einer fluessigkeit, insbesondere einer biologischen fluessigkeit
EP2759343A1 (fr) Unité de stockage d'un fluide et procédé pour la fabrication d'une unité de stockage d'un fluide
DE2611060A1 (de) Geraet mit abgemessenen fluessigkeitsvolumina

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: UNKNOWN

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20190606

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

17Q First examination report despatched

Effective date: 20210601

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

INTG Intention to grant announced

Effective date: 20230320

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

Free format text: NOT ENGLISH

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 502017015278

Country of ref document: DE

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

Free format text: LANGUAGE OF EP DOCUMENT: GERMAN

U01 Request for unitary effect filed

Effective date: 20230921

U07 Unitary effect registered

Designated state(s): AT BE BG DE DK EE FI FR IT LT LU LV MT NL PT SE SI

Effective date: 20230927

U20 Renewal fee paid [unitary effect]

Year of fee payment: 7

Effective date: 20231113

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20231124

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20231123

Year of fee payment: 7

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: ES

Payment date: 20231215

Year of fee payment: 7

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20231223

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230823

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20231123

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20231223

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230823

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20231124

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: CH

Payment date: 20231201

Year of fee payment: 7

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2959736

Country of ref document: ES

Kind code of ref document: T3

Effective date: 20240228

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230823

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230823

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230823

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230823

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20230823