EP3541516B1 - Device for receiving, dispensing, and moving liquids - Google Patents

Description

The invention relates to a device for receiving, dispensing, diluting or moving liquids and for adding liquid components, which can also be referred to as a microfluidic system. The device can also be referred to as a chip.

background

The uptake and delivery of liquids and gases and their movement, including mixing, in fluidic systems, particularly in microfluidic systems, often takes place via an externally connected pump, which is connected to the fluidic system via a fluidic interface, via syringe pumps integrated into the fluidic system or via diaphragm valves. All of these solutions require an appropriate operating device in order to be able to operate the pumps or valves and are not suitable for easily implementing functions such as the intake, delivery and/or movement of liquids in lab-on-a-chip systems.

The external pumps for manipulating lab-on-a-chip systems require a fluidic interface, which requires additional components and which, like all fluidic interfaces, involves the risk of leakage.

Syringe pumps integrated directly into fluidic systems avoid a fluidic interface to the outside, but require another element, the plunger, to move liquids.

Diaphragm valves offer the advantage that they do not require a fluidic interface or additional components and only require a preformed recess and a movable cover for actuation. These are designed in such a way that they can be operated both pneumatically and mechanically. These diaphragm valves are usually operated using a corresponding control gear.

Taking up and dispensing liquids, distributing them to different reaction cavities, moving liquids and adding reaction components requires manual handling steps or corresponding automation of these steps using large machines. This is done manually when taking samples and adding reagents by means of pipetting, mixing and incubating is done, for example, by shaking titer plates and for adding reagents, these are to be taken from appropriate storage containers. Both manual handling and automated handling requires a larger number of handling steps, additional equipment such as pipettes or pipetting machines and the possibility to store the corresponding reagents.

In microfluidic systems, handling usually takes place via external pumps and requires a device to control the system.

WO 2015/084458 A2 discloses a reagent dispensing device comprising: a first substrate having a first surface, the first substrate including one or more first chambers disposed within the first substrate; a first resistor unit disposed adjacent the surface of the first substrate and fluidly connected to at least one of the one or more first chambers, the first resistor unit including a first reagent.

WO 2015/077412-A1 , WO 2010/091246 A2 , DE 10 2009 032744 A1 and WO 2007/057744 A2 disclose fluidic systems with chambers, channel systems and flexible regions.

The present invention combines all handling steps, including the pre-storage of reagents, on a component that can also be operated manually.

Summary of the Invention

The object of the invention is to be able to take up, dispense, dilute, transport and/or mix liquids both manually, i.e. without additional aids, and with appropriate devices. This should preferably be possible in fluidic systems without an external pump or suction device, preferably also manually. A special feature of the system is that liquids can be taken up and dispensed multiple times and that desired volumes of the liquid taken up or dispensed can be precisely controlled.

The object is solved by the features of independent claim 1. Advantageous configurations are specified in the dependent claims.

A fluidic system is specified, comprising a structured component with a chamber and a channel system, which are sealed in a fluid-tight manner by a component, the chamber being fluidically connected to the outside world via the channel system and a fluidic interface. The device has a flexible or moveable area that can be moved into the chamber area or beyond a level of the chamber. The level of the chamber is the upper limit of the chamber on the side to the chamber, i.e. the underside of the component closing the chamber. Due to the movement of the flexible area, liquids or gases can be taken up or released through the fluidic interface or moved in the fluidic system. The movable area can be moved manually or with an appropriate operating device. One option is to push the flexible area in or move it up to different positions. The possibility of defined fluid delivery and intake through the combination of the chamber with a small channel system, the multiple intake and delivery of liquids and the possibility of manual operation are particularly advantageous.

The fluidic system preferably has an interface for a liquid reagent reservoir.

The configuration of the component closing the structured component as a film is particularly advantageous, the film being the movable component at the same time due to its intrinsic flexibility.

The liquid taken up is diluted or the reagents are supplied by emptying a liquid reservoir which is connected to the structured component and which can be configured as a blister. The liquid intake and liquid removal can be influenced by the external geometry of the fluidic interfaces.

The volume can be defined by the corresponding outlet geometry of the fluidic interface, and this volume definition can be further influenced by a surface modification of the fluidic interface.

A further fluidic system is also specified, comprising a structured component with a chamber and a channel system which are sealed with a further component, the chamber being fluidically connected to the outside world via the channel system and a fluidic interface. The flexible area is formed by the walls of the chamber.

It is particularly advantageous here that pressing the chamber laterally also allows the liquid to move or the compression effect can be intensified by the flexible chamber walls.

A further fluidic system is also specified, comprising a structured component or structured component and a further component which seals the chamber and channel system tightly and the chamber is connected to the outside world via the channel system and the fluidic interface. The structured component is designed in such a way that the chamber floor is flexible and can be pressed in or expanded.

It is particularly advantageous in this embodiment variant that the base can be designed to be particularly flexible and production by means of two-component injection molding is possible, so that a flexible component can be injected together with another component. Alternatively, the base material of the structured component can also be sufficiently flexible to ensure the functionality of the component. An assembly of the flexible area in the structured component is also possible.

The chamber can be connected to a fluidic interface via a further channel system, it being possible for one of the fluidic interfaces to be closed with a cap. The closure with a cap also prevents liquid leakage at this point.

The integration of valves, for example capillary stop valves, which act by changing the capillary diameter, allows defined volumes to be taken up.

A valve function is preferably created by local modification of the surface or the function of existing geometrically acting valves is further reinforced by a surface modification in the valve area.

What is particularly advantageous in this embodiment variant is that when liquid is taken up through the second fluidic interface, venting can take place and, moreover, liquid can be taken up and discharged at different points. The closure with a cap also prevents liquid leakage at this point. Furthermore, a corresponding positioning of the fluidic system so that the dispensing fluidic interface is inclined downwards during the dispensing of liquid is advantageous.

Preferably, the fluidic system includes a venting option for the chamber, which can be via an additional channel communicating with the outside world or a gas-permeable membrane, and this venting device can optionally be closed.

The fluidic system preferably contains an inlet channel that has a passive stop function, for example a capillary stop valve, a channel narrowing or a corresponding surface modification, and either via capillary action, which can be enhanced by surface modifications in the area to be filled, or by one brought about by the moving components Change in chamber volume absorbs a defined amount of liquid.

The recording of very precise volumes without using expensive pipetting units is particularly advantageous here.

In a preferred embodiment, the fluidic system contains an additional reagent reservoir. This can be formed as a blister, for example.

It is particularly advantageous here that several fluids or dry reagents can be mixed with one another and the reagent can be used to transport liquid that has been taken up or that has been placed in the system.

Dry reagents are preferably introduced into the structured component, which can be absorbed by the fluids flowing through and mixed with them.

A reagent is preferably placed at a defined point, which colors the liquid flowing over and thus indicates that the position at which the reagent is placed has been reached, and thus indicates that a specific volume or a residence time has been reached.

A magnification function is preferably introduced into the structured component at a defined position, which takes place e.g. in the form of a lens integrated into the structured component, in order to be able to better track the reaching of certain positions in the channel system through the liquid and also to be able to read color reactions as indicator reactions better can.

Longer channel elements are also preferably introduced into the course of the fluid as flow limiters in order to enable controlled fluid intake and discharge.

In a preferred embodiment, the reagent reservoir is in the form of a blister. The reagent reservoir preferably has a blister seat which has pointed elements which pierce the blister which is seated above and which is connected in a liquid-tight manner. This embodiment has a flap which, via guide elements in the blister seat, allows the flap to be inserted in a defined manner and thus allows a defined volume dosage. The volume dosing can also take place in several stages thanks to the special design of the guide elements.

The liquid-tight closure of the fluidic interface for liquid intake is useful, for example via a cap. The cap can also be provided with a transport element, e.g. In addition or as an alternative, the cap can also have a flexible area which, after it has been put on, can be pressed in or pulled out in order to move the liquid in the channel or in the channel system. When pressed, the liquid is pushed further into the channel. When the flexible area is pulled out, liquid is conveyed out of the channel in the direction of the fluidic interface. This also allows small movements to be generated.

It is particularly advantageous here that defined volumes of liquid can be dispensed from the blister and this can also be done manually with great precision. This means that an exact mixing ratio can be set in combination with a defined volume intake.

In a preferred embodiment, the fluidic system has a long channel leading to the chamber. This long channel is particularly advantageous since it allows the rate at which liquid is taken up to be adjusted and reagents can be introduced into the channel which resuspend optimally as they are carried along in the channel for a long time.

In a preferred embodiment, the long channel has additional expansions towards the chamber. This embodiment is particularly advantageous since reagents can be prepackaged in the expansions and improved mixing can take place due to a different flow profile.

In a preferred embodiment, the fluidic system contains a cavity or detection chamber for optical readout and/or for reaction, which can preferably also have different depths. It is particularly advantageous here that optical detection can be carried out directly and the dynamic range can also be increased if the detection chamber is designed with several depths.

In a preferred embodiment, the fluidic system contains a lateral flow strip, the filling of which is made possible by the operation of the chamber. One design variant includes a ventilation membrane, another a ventilation channel. The possibility of liquid aspiration, which can be operated manually, with the direct option of reading out via the lateral flow strip, is particularly advantageous. Specifically targeted venting options allow the combination of the vacuum-driven flow achieved through the chamber with the subsequent liquid movement through the suction effect of the lateral flow strip.

In a preferred embodiment, the fluidic system contains more than one chamber, which are connected to one another via a channel system and can be arranged in one or more levels. It is particularly advantageous that forwarding and pushing back and forth as well as active mixing are made possible by changing the chamber volume through the flexible elements.

In a preferred embodiment, the fluidic system contains attachments on the flexible components, which are either located outside the chamber or extend into the chamber. A precise definition of the volume to be taken up or released is particularly advantageous here, which is therefore independent of the strength or finger size of the user even when operated manually.

In a preferred embodiment, the fluidic system has reagents that are placed in the chamber. It is particularly advantageous here that the chamber not only serves to move the liquid, but that the chamber volume can be used directly for dissolving, reacting and mixing reagents. In particular, dry reagents that are provided allow a particularly advantageous use of the chamber.

In a preferred embodiment, the cap for emptying the blister is connected directly to pressure elements for moving the flexible area, possibly also realized in one piece.

In a preferred embodiment, mixing is possible by moving elements introduced into the chamber, such as balls or rods, which can also be magnetic. The mixing can be additionally enhanced by or structural elements in the structured component. A particular advantage here is that the simple structure of the system allows particularly effective mixing in the chamber.

In a preferred embodiment, mixing takes place in the chamber by manually moving the fluidic system. A particular advantage here is that the simple structure of the system allows manual use.

In a preferred embodiment, mixing takes place in the chamber by means of an on-board mixing mechanism. It is particularly advantageous here that efficient mixing can take place.

In a preferred embodiment, the channel systems themselves contain alignment marks, or alignment marks are attached next to, below or above the channel system, which enable volume information to be given. This marking is particularly advantageous, similar to a ruler, as it allows the user to read the volume taken up or dispensed and to end or continue taking up or dispensing volumes in order to add, dispense or move defined volumes.

In a preferred embodiment, it is possible to take up or dispense liquid several times. It is particularly advantageous here that the fluidic system can be used to take up and dispense liquids multiple times.

In a preferred embodiment, fluidic interfaces are provided on the structured component, which point in different directions, for example perpendicular to the plane of the fluidic system or starting from the fluidic system at a specific angle. It is particularly advantageous here that a special geometry allows liquids to be applied or dispensed to specially shaped surfaces or vessels.

In a preferred embodiment, several fluidic interfaces are provided.

This is particularly advantageous since liquids can then be dispensed and received at different points simultaneously or one after the other.

In combination with a distribution system, it is possible to pick up and drop off at several points at the same time or one after the other. When using a pure distribution system, a simultaneous delivery or intake of liquids via the movement of the flexible elements.

In a preferred embodiment, the intake or delivery of the liquids is controlled via membrane valves, see FIG. This is particularly advantageous since it allows fluid to be taken up or released individually at different fluidic interfaces as a result of the movement of the flexible elements in the chamber.

A special design is the integration of passive valves in the individual distribution channels in order to ensure uniform filling and thus uniform liquid transport and thus, for example, the delivery of the same volumes.

In a preferred embodiment, the uptake and delivery of the liquids is controlled via rotary valves. The rotary valves preferably have a rotary valve seat (28a) and a rotating rotary valve body (28b) with a connecting channel that connects the different parts of the channel system. This is particularly advantageous since it allows fluid to be taken up or released individually at different fluidic interfaces as a result of the movement of the flexible elements in the chamber.

In a special embodiment, the fluidic system is designed as a microfluidic system. The structured component is preferably and essentially made of plastic.

In the case of the flexible element, the entire component can be produced as a plastic film, for example. However, it is also possible to use a flexible plastic such as silicone or TPE that is introduced into the other components, or a movable mechanical element made of any material.

The fluidic system is also referred to as a thumb pump, since the flexible component can be operated particularly easily with the thumb.

Brief description of the drawings

Fig. 1a bis 1c

ein fluidisches System gemäß einer Ausführungsform.

Fig. 2

ein fluidisches System gemäß einer alternativen Ausführungsform.

Fig. 3

ein fluidisches System gemäß einer weiteren alternativen Ausführungsform.

Fig. 4a bis 4c

fluidische Schnittstellen eines fluidischen Systems gemäß Ausführungsformen.

Fig. 5a bis 5f

Druckelemente eines fluidischen Systems gemäß Ausführungsformen.

Fig.6a und 6f

ein fluidisches System gemäß einer weiteren Ausführungsform.

Fig.7a und 7b

ein fluidisches System gemäß einer noch weiteren Ausführungsform.

Fig.8a bis 8e

einen Ausdrückmechanismus eines fluidischen Systems gemäß Ausführungsformen, die nicht unter den Schutzumfang der Erfindung fallen.

Fig.9a und 9b

ein fluidisches System mit Aufweitungen und Detektionskammer gemäß Ausführungsformen.

Fig.10a bis 10c

ein fluidisches System mit einem Lateral-Flow-Streifen gemäß Ausführungsformen.

Fig. 1 1

ein fluidisches System gemäß einer anderen Ausführungsform.

Fig.12a bis 12d

ein fluidisches System mit einem Verteilersystem gemäß Ausführungsformen.

Fig.13

ein fluidisches System gemäß einer anderen Ausführungsform.

Fig.14a, 14b

ein fluidisches System mit einer Vergrößerungsvorrichtung gemäß einer Ausführungsform.

Fig.15a- 15c

zeigen ein fluidisches System mit Flussbegrenzern gemäß Ausführungsformen.

Fig. 16

zeigt eine Ausführungsform des Chips mit Kappe in einer Ansicht von oben

In the figures show:

Figures 1a to 1c

a fluidic system according to an embodiment.

2

a fluidic system according to an alternative embodiment.

3

a fluidic system according to a further alternative embodiment.

Figures 4a to 4c

fluidic interfaces of a fluidic system according to embodiments.

Figures 5a to 5f

Pressure elements of a fluidic system according to embodiments.

6a and 6f

a fluidic system according to another embodiment.

Figures 7a and 7b

a fluidic system according to yet another embodiment.

Figures 8a to 8e

an expression mechanism of a fluidic system according to embodiments not falling under the scope of the invention.

Figures 9a and 9b

a fluidic system with flares and detection chamber according to embodiments.

Figures 10a to 10c

a fluidic system with a lateral flow strip according to embodiments.

Figure 1 1

a fluidic system according to another embodiment.

Figures 12a to 12d

a fluidic system with a distribution system according to embodiments.

Fig.13

a fluidic system according to another embodiment.

Figures 14a, 14b

a fluidic system with an augmentation device according to an embodiment.

15a-15c

show a fluidic system with flow restrictors according to embodiments.

16

Figure 12 shows an embodiment of the capped chip in a top view

Detailed description

The present invention describes a fluidic system with a chamber, which has a flexible or movable part, usually the base or lid, but also movable walls in special embodiments, which, by lifting or pressing down, allows the intake, delivery, displacement, dilution or Allows mixing of liquids or gases connected to the chamber via at least one channel or port.

The chamber and the movable part are designed in such a way that a predetermined but adjustable volume of the chamber is displaced by a movement of the movable part from its starting position. In this way, predetermined volumes can be taken up or released when the movable part is returned to another position or to the starting position in the chamber. In other words, the volume is predetermined by the properties of the fluidic system or can be adjustable by designing the fluidic system according to the invention.

Figures 1a to 1c show an embodiment of the fluidic system. Figures 1a and 1c show a plan view of the fluidic system, Fig. 1b shows a cross-sectional view of the fluidic system.

The fluidic system has a structured component 1 with a chamber 2 , the chamber 2 being connected to a channel system 3 . The structured component 1 is essentially flat or plate-like. In other words, the structured component 1 has a first main side and a second main side, which face each other in parallel. The chamber 2 and the channel system 3 are formed on the surface of the structured component 1 on the first main side. In other words, the chamber 2 and the channel system 3 are let into the surface of the structured component 1 on the main side. The chamber 2 and the channel system 3 therefore represent a depression on the surface of the structured component 1. The first main side is, for example, a top side, the second main side is, for example, a bottom side of the structured component 1. Between the top side and the bottom side of the structured component 1 side surfaces of the structured component 1 are arranged. The structured component can be cuboid, for example. The structured component 1 can also be disk-shaped. However, the patterned member can take any shape as long as it is substantially flat.

The structured component 1 can be designed as a platform, for example. The structured component 1 can also be referred to as a structured component 1 . The structured component 1 can be of flat design.

The chamber 2 or the channel system 3 therefore has an upper side which corresponds to the upper side of the structured component 1 . An underside of the chamber 2 or of the channel system 3 is formed within the structured component 1 . The underside of chamber 2 can also be referred to as chamber floor 7 . The interior of the chamber 2 is formed between the top of the chamber 2 and the bottom.

The chamber 2 or the channel system 3 can be designed as a depression in the structured component, e.g. on the upper side or the underside of the structured component 1. The chamber 2 and the channel system 3 can be designed as depressions of different depths.

The chamber 2 or the channel system 3 is fluidically connected to the outside world via a fluidic interface 5 . In other words, the fluidic interface 5 is an opening in the channel system on a side surface of the structured component 1. The opening in the fluidic interface 5 can also be arranged on an upper side or underside of the fluidic system. As in Fig. 1a as can be seen, the structured interface 5 can protrude from a side surface of the structured component 1 as a projection. In this case, it is possible to use the fluidic system to take up liquid directly from a liquid surface, for example liquid that is in an open-topped container, by dipping the projection into the liquid and moving the flexible or movable component.

The fluidic system can have multiple fluidic interfaces 5 which are each connected to the channel system 3 . The fluidic interfaces 5 can be at different Be arranged surfaces of the structured component 1, such as the top, bottom, or side surfaces. In other words, the openings of the fluidic interfaces 5 can point in different directions, ie they can have different orientations with respect to the center point of the structured component 1 .

A second component 4 closes the channel system 3 and the chamber 2 in a liquid-tight and gas-tight manner, so that liquids and gases can only be supplied and discharged via the fluidic interface 5 . In other words, the second component 4 is arranged on the surface of the structured component 1 in such a way that it closes the chamber 2 and the channel system 3 on the upper side of the structured component 1 . The second component 4 can, for example, be glued onto the structured component 1 or welded to the structured component.

In other words, the interior of the chamber 2 is delimited by the underside of the second component 4 on the upper side of the chamber 2 . The chamber 2 can have a substantially flat oval, rectangular or round shape. The chamber 2 or the interior of the chamber 2 is thus defined on the one hand by the structured component 1 and on the other hand by the second component 4 .

In this case, the second component 4 is flexible or the second component has a flexible or movable area 6 . As in Fig. 1b shown, the flexible area 6 of the second component 4 is arranged above the chamber 2 as a direct part of the second component 4 . Alternatively, the flexible or movable area 6 can be designed as a further component of the fluidic system. The flexible or moveable area 6 of the second component 4 should be arranged at least on an area of the chamber 2 or on the outside of the chamber 2 .

The second component 4 can be formed, for example, as a film or strip and can be made of plastic or metal.

Alternative embodiments of the fluidic system are in 2 and 3 shown. According to the 2 In the alternative embodiment shown, the structured component 1 has a flexible area 7 below the chamber. In other words, the flexible area 7 is arranged between the chamber floor and the underside of the structured component 1 . The flexible area 7 can either be implemented by applying a further component to the structured component 1 or directly via the material properties of the structured component 1 itself or by manufacturing it from more than one material, for example by multi-component injection molding.

Another alternative embodiment is 3 shown. According to the further alternative embodiment, the structured component 1 is with the second component 4 and beyond closed with another component 8, one or both components 4 and 8 can have a flexible or movable area. In other words, the second component 4 is arranged on the upper side of the structured component 1 . That is, the top of the chamber 2 is closed with the second component 4 . The further component 8 is arranged on the underside of the structured component 1 . That is, the underside of the chamber, ie the chamber floor, is closed with the additional component 8 . As in 3 shown, a flexible area 9) is shown in the further component 8.

The structured component 1 is preferably formed with a cover film that has sufficient flexibility to be pressed in and lifted above or below the chamber 2 .

The chamber 2 is preferably designed in such a way that the flexible area(s) 6 , 7 , 9 does not fill the entire chamber 2 when it is pressed into the chamber 2 . In other words, when the flexible area 6, 7, 9 is pushed into the chamber 2, the flexible area does not close flush with the bottom of the chamber. That is, liquid or gas located in the chamber 2 is not completely displaced from the chamber 2 by the flexible region 6, 7, 9 being pushed in. Furthermore, a tight seal of the flexible areas 6, 7, 9 with the chamber floor or adjoining channel systems 3 is not necessary for the functionality.

An exemplary operation of the in Figures 1A to 1C illustrated embodiment is described below:
Taking up liquid: In order to take up liquids/gases into the fluidic system, more precisely into the chamber 2 of the fluidic system, the flexible area 6 is moved manually, eg with a finger of a user, or by means of an operating device from the starting position depressed. In other words, the flexible portion 6 is pressed into the chamber 2 from its initial position by pressure. That is, the flexible area 6 is pressed into the interior of the chamber 2 via the upper side. By pressing the flexible area 6 into the chamber 2, the interior space of the chamber 2 is reduced. The fluidic interface 5 is then immersed in a liquid. The flexible area 6 moves either automatically, due to the material properties of the flexible area 6, partly or completely back into the starting position, or is moved back into the starting position by a movement of the operating device, eg suction or lifting. In other words, the interior space of the chamber is increased again by moving the flexible area 6 back into the starting position. The increase in volume of the interior creates a negative pressure in the chamber 2 or in the adjacent channel system 3, which is connected to the liquid via the fluidic interface. That is, liquid is drawn into the fluidic system by the negative pressure. In other words, through the A part of the liquid is first drawn into the channel system 3 and, if the negative pressure is sufficiently large, then also into the chamber 2 under negative pressure. Liquid is thus absorbed into the fluidic system. By adjusting the volume of the interior of the chamber 2 displaced by pressing down the flexible area 6 and/or by returning the flexible area 6 to the starting position in a defined manner, the volume of the liquid taken up or the positioning of the liquid in the channel system 3 or in of chamber 2 of the fluidic system.

Mixing of liquids: The liquid taken up is mixed by first drawing liquid into chamber 2, i.e. liquid is first taken up into the fluidic system. Then either the flexible component 6 is moved or the fluidic system itself is moved. The fluidic system is moved, for example, by tilting the fluidic system multiple times. Rapid shaking should be avoided to prevent air bubbles from forming in the absorbed liquid.

Delivery of liquids: The delivery of liquids from the fluidic system takes place in that the flexible component 6 or the flexible components are pressed into the chamber 2 . In other words, the volume of the interior space of the chamber 2, which is delimited by the flexible component, is reduced by the flexible component being pressed in. The liquid, which is located either in the chamber 2 or in the channel system 3, is discharged from the fluidic system according to the volume displaced by the movement of the flexible area 6, ie by the flexible area 6 being pressed into the chamber 2. That is, the displaced liquid is released from the chamber 2 via the channel system 3 through the fluid interface 5 . The volume of the liquid dispensed may correspond to the volume of the interior of the chamber 2 by which the chamber is reduced by pushing in the flexible portion. Volumes of liquid can be dispensed multiple times. The multiple delivery can take place in that the flexible area 6 , 7 , 9 is gradually pressed further and further into the chamber 2 or the interior of the chamber 2 . The multiple delivery can also take place in that the flexible area 6, 7, 9 is first pressed into the chamber 2 and that the flexible area 6, 7, 9 then moves out of the chamber 2 either independently as described above or with the help of a Operating device is moved out of the chamber 2. The outward movement is accompanied by a backflow of at least part of the liquid in the channel system 3 connected to the chamber 2 . The withdrawal is followed by a repeated depression of the flexible portion 6, 7, 9 into the chamber 2 for renewed liquid delivery. In other words, by repeatedly and alternately pressing the flexible area 6, 7, 9 into the chamber 2 and moving it out of the chamber 2, a pumping movement or a pumping functionality is carried out. This leads to repeated and alternating liquid intake and liquid discharge.

Closure of the fluidic interface 5 for sampling: The fluidic interface 5 for sampling is closed by a cap 14 . Due to the design of this cap 14, the volume in the channel system 3 can also be displaced by integrated projections.

One fluidic interface 5 is preferably configured as an input 5.1 and another fluidic interface 5 is configured as an output 5.2 of the fluidic system. The entrance 5.1. and output 5.2 are preferably formed on the structured component 1. The two fluidic interfaces 51 and 5.2 are formed on one side, preferably on an end face or narrow side of the chip (fluidic system). This means that the input and the output are arranged on one side of the system. This makes it possible to close the input and output with a cap 14, which is also referred to as a jumper.

The cap 14 is preferably attached to the fluidic system, preferably to the structured component 1 . One or more caps 14 may be attached.

In a preferred embodiment, only one cap 14 is provided, which can be plugged onto either the input 5.1 or the output 5.2. A liquid can then be selectively taken up at the inlet or liquid can be dispensed at the outlet.

The one or more caps 14 are attached to the chip via a tab 44 .

Addition of liquid: By completely or partially emptying a liquid reservoir 16, the taken sample is transported by a liquid and diluting or adding reagents becomes possible.

Due to its flexibility, the flexible region 6 can thus be pressed under a plane defined by the upper side of the structured component 1 into the chamber 2, more precisely into the interior of the chamber 2, by external pressure. On the other hand, the flexible area 6 can be pulled out of the interior of the chamber 2 again by pulling from the outside, e.g. That is, it can be moved beyond the plane defined by the top of the structured component 1.

These basic functionalities, i.e. the intake of liquid into the fluidic system, the release of liquid from the fluidic system and the mixing of liquid contained in the fluidic system, result in the following characteristics for the fluidic system:
It is possible to absorb, dilute, dispense, dose or transport liquids. Liquid that has been added to the fluidic system can use the fluidic system can be transported and stored. Multiple intake and multiple dispensing of liquids is possible. Mixing of liquids is possible.

The design of the fluidic system according to the above-described embodiments means that the fluidic system can be used as a pipette with functions of liquid intake, liquid delivery and multiple intake and delivery of liquids due to the design of the chamber 2 and the flexible areas 6, 7, 9. The operation can be carried out completely manually without additional tools or by means of an operating device.

FIGS. 4a to 4c show embodiments of the fluidic interface 5. The embodiments of the fluidic interface 5 according to FIGS. 4a to 4c differ in their geometry. More precisely, the shown embodiments of the fluidic interface 5 each have an outlet 10, the shape of the outlet 10 being different in the shown embodiments. The special or defined geometry of the outlet and/or a surface modification or a material composition of the outlet 10 of the fluidic interface can be used to set the volume of a droplet of the liquid dispensed at which the droplet breaks away from the outlet. Due to the defined geometry of the outlet 10 of the fluidic interface 5, volumes, i.e. desired volumes, of the liquid drop of the liquid dispensed can be preset. That is to say, the geometry of the outlet 10 of the fluidic interface 5 is also decisive for the volume of liquid dispensed. In other words, when liquid is to be dispensed from the fluidic system, the flexible area 6 , 7 , 9 is pressed into the chamber 2 so that a drop of liquid forms at the outlet 10 of the fluidic interface 5 . The flexible area 6 , 7 , 9 is pressed further into the chamber 2 until the drop of liquid tears away from the outlet 10 . The pressing in of the flexible area 6, 7, 9 or the dispensing of liquid can then be terminated. Alternatively, the flexible portion 6, 7, 9 can be pushed further into the chamber 2 to create another drop of liquid.

Figures 5f to 5f 12 show pressure elements of the flexible regions according to various embodiments. The flexible areas 6, 7, 9 can have pressure elements 11, 12, 13 in order to ensure that the flexible areas 6, 7, 9 are pressed into the chamber 2 in a defined manner or that the flexible areas 6, 7, 9 are pulled out or moved out in a defined manner to allow the chamber 2. In other words, in order to prevent differences due to a person-dependent force or finger size even during manual actuation or manual operation, pressure elements 11, 12, 13 can be arranged or applied to the flexible areas 6, 7, 9. In other words, the pressure elements 11, 12, 13 can ensure that the same volume of the interior of the chamber 2 is always displaced when the flexible region 6, 7, 9 is pressed into the chamber 2. The pressure elements 11, 12, 13 can be operated either manually, for example with a finger, or by an operating device. The Printing elements 11, 12, 13 can be materials applied to the flexible area 6. For example, the pressure elements 11 can be designed as a silicon hemisphere, such as in Figures 5a and 5b shown. Alternatively, the pressure elements 12 can be manufactured directly with the flexible area 8, for example by multi-component injection molding, as in Figures 5b and 5c shown. Alternatively, a defined impression can also be achieved using pressure elements 13, which are protruding elements in the structured component, as in Figures 5e and 5f shown. In other words, the in Figures 5e and 5f The pressure elements 13 shown are arranged in the chamber 2 of the fluidic system, for example on the chamber floor, and protrude into the interior of the chamber 2 . The movement of the flexible area 6 when it is pressed into the chamber 2 can thus be limited by means of the pressure elements 13, so that only a maximum volume of the interior space is displaced. Figures 5a, 5b, and 5e each show the initial state of the flexible area 6, 7, 9, ie the state when no force or pressure is exerted on the flexible area 6, 7, 9. Figures 5b, 5d and 5f each show a position before liquid is taken up or during liquid discharge, ie a position of the flexible region 6, 7, 9 when it is pressed into the chamber 2.

Figures 6a and 6b show further embodiments of the fluidic system. More precisely show Figures 6a and 6b a fluidic system which has two separate fluidic interfaces 5 . As in Figures 6a and 6b shown, the fluidic interfaces 5 are arranged on different, more precisely opposite, side surfaces of the structured component 1 and protrude from the respective side surfaces. Here, liquid can be taken up through one of the two fluidic interfaces 5 and liquid can be removed through the other of the two fluidic interfaces 5 . As in Figure 6b shown, the fluidic interfaces 5 can also be closed by a cap 14 in order to prevent contamination or liquid from escaping from the fluidic interface 5 . The liquid contained in the fluidic system can be transported and stored in a particularly safe and simple manner through the cap 14 . In other words, the cap 14 can be placed on the fluidic interface 5, more precisely, on the opening formed by the fluidic interface 5 in a side surface of the structured component, and the fluidic interface 5 can be closed off in a fluid-tight manner.

As in Figures 7a and 7b shown, the fluidic system can be expanded by a liquid reservoir 16 . The liquid reservoir 16 is connected to the channel system 3 or to the chamber 2 via a channel. The channel can be part of the channel system 3. The liquid reservoirs 16 can be formed, for example, by one or more so-called blisters, ie liquid-filled compartments which can be opened, for example by piercing, and which are mounted on the fluidic system in a liquid-tight manner. A liquid intake from the blister is achieved by depressing the flexible portion 6 as described above and moving the flexible portion 6 out of the chamber 2, with the resulting negative pressure in the Chamber 2 and the channel system 3 is received via the connected channel liquid from the blister in the channel system 3 and the chamber 2. A cap 14 is placed on the fluidic interface to prevent liquid from escaping from the fluidic interface 5 if further liquid is pushed into the chamber 2 by emptying the liquid reservoir 16 in the channel system 3 and the liquid from the liquid reservoir 16 is also pushed in chamber 2 is flowing. In other words, liquid that is received into the fluidic system from the outside and is located in the channel system 3 or in the chamber can be mixed with the liquid in the liquid reservoir 16 . The mixing can be facilitated or intensified by placing the cap 14 on the fluidic interface, since with the cap 14 in place the negative pressure resulting from the movement of the flexible area 6 acts on the liquid in the liquid reservoir 16 .

The liquid reservoir 16 may also be referred to as a reagent reservoir or liquid reagent reservoir and may contain any type of liquid.

The liquids can be mixed by a movement of the fluidic system, a movement of the flexible area 6, 7, 9 or introduced mixing elements. The mixing elements, e.g. silicone balls, can be created by manually moving the fluidic system. Alternatively or additionally, the mixing can be done by means of elements made of magnetic materials, which are moved by an external mixing device.

Figures 7a and 7b show an embodiment of the fluidic system that combines two types of liquid intake. On the one hand, the sample is taken up, for example, through the fluidic interface 5 serving as a liquid inlet, by moving the flexible area 6, 7, 8 of the chamber 2 into the chamber 2 and moving the flexible area out, as described above. Alternatively, liquid can be taken up automatically into the fluidic system via passive filling, ie by means of capillary forces of the channel system 3 at the fluidic interface 5 . The suction effect, which is caused by the negative pressure or by the capillary forces, and thus the filling speed, can be intensified or accelerated by a surface modification, for example hydrophilicization of the channel surface of the channel system 3.

Furthermore, by means of passive valves in the channel system 3, for example capillary stop valves and channel constrictions 41, see Figure 7a , the channel system 3, the volume of the recorded liquid can be determined. A defined quantity of liquid is thus taken up, with a closure cap preventing the liquid from escaping when the liquid reservoir 16 is emptied.

Figures 8a to 8e 12 show a liquid reservoir 16 ejection mechanism according to embodiments not falling under the scope of the invention. . The squeezing mechanism can be designed, for example, as a flap 19, with the latching of the flap 19, as in Figure 8d shown the introduction of a defined amount of liquid from the liquid reservoir 16 into the channel system 3 of the fluidic system, whereby a defined mixing ratio of the liquid from the liquid reservoir with the liquid contained in the fluidic system is achieved. Figure 8d shows a state in which the flap 19 presses the liquid reservoir 16 onto the fluidic interface 5 of the channel of the channel system 3. This principle can be extended to other liquid reservoirs 16 and can therefore be used for multiple mixtures.

Figure 8a shows an ejection mechanism with a seat 17, which can be designed as a blister seat and has piercing elements 18, for example small tips.

Figure 8b 14 shows an embodiment of an ejection mechanism, wherein the seat 17 has detents 19 and the flap 19 is movably mounted on the detents 20 of the seat 17 in a hinge-like manner. As in Figure 8b shown, the liquid reservoir 16 is located on the flap. the inside Figure 8b The push-out mechanism shown can also have push-through elements 18 (not shown). One of the latching lugs 20 serves as a hinge and another of the latching lugs 20 serves as a latching surface or bearing surface for the cap 19 in order to limit rotation of the cap 19 in this way. This means that when the flap 19 is closed, the liquid reservoir 16 is punctured and the liquid from the liquid reservoir can be taken up into the channel system 3 of the fluidic interface. By limiting the rotation of the flap 19 by the locking lugs, a defined or predetermined amount of liquid can be released from the liquid reservoir to the fluidic system.

The seat 17 can also be referred to as the reservoir interface.

Figure 8c FIG. 1 shows an embodiment of the ejection mechanism in which the liquid reservoir 16 is arranged on the surface of the structured component 1. FIG. In this case, the flap 19 can have a bulge or projection, as in FIG Figure 8d shown, so that the liquid reservoir 16 is expressed by the projection when the flap 19 is closed. Figure 8d shows the depressed ejection mechanism, in this case the flap 19..

Figure 8e 14 is a plan view of a seated ejector mechanism 17 according to one embodiment.

Figures 9a and 9b show a fluidic system with a long channel system 3. As in Figures 9a and 9b shown, the channel system 3 meanders between the fluidic interface 5 and the chamber 2, whereby the length of the channel system 3 is increased. This creates a dwell stretch created for the liquid contained in the fluidic system. The dwell zone can be filled with reagents, for example dried reagents. As a result, a long channel system 3 can be formed. The channel system 3 can also have widenings 22 for better mixing, as in Figure 9a shown, or have a further passive mixing element. As shown, the expansions can be elongate or in the direction of flow in the channel system 3 . Liquid or reagents can be introduced into the expansions 22, which are mixed with the liquid taken up into the channel system 3 or the fluidic system or mixed with the liquid discharged from the fluidic system. The channel system 3 can also have an optical detection chamber or reaction chamber 22, 21, as in Figure 9b shown. A configuration of the detection chamber 21 at different depths is particularly advantageous in order to expand the dynamic range of the measurement. In other words, the detection chamber 21 can be let into the structured component 1 at different depths, so that it has, for example, detection chamber floors of different depths in steps.

Another option for expanding the chamber functionality is the introduction of a lateral flow strip 23, as in Figures 10a to 10c shown, which can be filled in a defined manner by means of the pumping function of the fluidic system. A combination of filling by the pumping effect of the chamber 2 in the manual operation described above or by means of an operating device and the suction effect of the lateral flow strip can also take place. As in Figures 10a to 10c shown, the lateral flow strip is embedded or introduced into a further chamber, which is also connected to the channel system 3 . The use of ventilation channels 25 or gas-permeable and liquid-tight membranes 24, which are respectively connected to the channel system 3 or the chamber of the lateral flow strip, for operating the system is particularly advantageous. This is the case, for example, for the gas-permeable and liquid-tight membranes 24 in Figure 10b and for vent ducts 25 in Figure 10c shown.

11 12 shows a fluidic system according to yet another embodiment. As in 11 shown, the structured component 1 has two chambers 2, which are embedded in the upper side of the structured component. The two chambers 2 are directly connected to one another via a first channel system 3 or a channel. The two chambers 2 are also each connected to the environment via a fluidic interface 5 via a respective second channel system 3 or channel. This configuration of the fluidic system can also be referred to as a chamber system combined with one another. The use of chamber systems combined with one another, which can then be used simultaneously as a mixing, reaction, pumping and/or dosing unit, is a further embodiment of the fluidic system.

Figures 12a to 12d show embodiments of the fluidic system with distribution systems 26. As in Figures 12a to 12d As shown, a chamber 2 is connected to a manifold system 26 at one end. The distribution system 26 can be part of the duct system 3 . The distribution system 26 has one or more channels leading away from the chamber 2 and branching out. The ends of the respective branched channels of the distribution system 26 are each connected to a fluidic interface 5 . As in the embodiments of the fluidic system of Figures 12a to 12d shown, one channel leads away from the chamber 2 and branches into four channels each, which are each connected to a fluidic interface. Through the movement of the flexible area 6, 7, 9 and the associated change in the chamber volume, the distribution systems allow fluid to be taken up or released simultaneously or sequentially.

Figures 12a and 12b show a fluidic system with a distribution system 26, the channel leading away from the chamber 2 branching off step by step, namely initially into two further channels. The two further channels then each branch into two further channels, so that the channel leading away from the chamber 2 branches into a total of four channels, which open into the respective fluidic interfaces 5 . In 12a all fluidic interfaces 5 are controlled or actuated simultaneously by a movement of the flexible area 6, 7, 9. As in Figure 12b shown, the branched channels of the distribution system 26 can have diaphragm valves 27 . The use of diaphragm valves 27 requires the diaphragm valves 27 to be pressed in and sealed in a liquid-tight manner in order to close the respective channels individually or together and thus to be able to take in or release liquid via the fluidic interfaces 5 . In other words, the liquid flow within the respective channels can be controlled in a targeted and defined manner by means of the membrane valves 27 . That is, the individual fluidic interfaces 5 can be specifically controlled or actuated by means of the membrane valves 27 . That is, they can be controlled independently of one another. The diaphragm valves 27 can be or can be brought into a state that does not allow liquid flow in the respective channel, a state that allows an unimpeded liquid flow in the respective channel and/or a state that allows a reduced liquid flow in the respective channel being controlled. In this way, a defined and/or simultaneous liquid intake or liquid discharge can be controlled in a targeted manner via the respective fluidic interfaces 5 .

Figures 12c and 12d show an embodiment of the fluidic system with a distribution system 26, in which the channel leading away from the chamber 2 branches at one point in a star shape into four further channels. As in Figure 12c As shown, a rotary valve 28 can be arranged at the point of branching, which is operable from the outside manually or by means of a device. With the help of the rotary valve 28, a targeted flow of liquid can thus be connected between the channel leading away from the chamber 2 and one or more channels with the branched channels, ie channels connected to the fluidic interfaces 5. The body of the rotary valve 28 may itself have one or more recessed channels 29 which, when appropriately positioned at the point of bifurcation, form the seat 28a of the rotary valve 28 can connect the branched or connected channels with each other. Depending on the design of a distribution channel 29 integrated in the rotary valve body (28b), the option with a rotary valve 28 allows sequential or parallel fluid intake or discharge via one or more fluidic interfaces 5, which in turn is controlled by changing the chamber volume. It is also possible to combine one or more membrane valves 27 and/or rotary valves 28 in one fluidic system. This means that the individual fluidic interfaces 5 can also be specifically controlled or actuated by means of rotary valves 28 . That is, they can be controlled independently of one another.

In general, for the fluidic system according to the present invention, all processes described for the use of liquids are equivalent to gases and a combination of liquid and gaseous substances is also possible with this fluidic system, for example the targeted supply of gases in liquids.

Another embodiment is in 13 shown. Here the structured component 1 has a flexible area 7 below the chamber 2, which is realized either by applying another component in the structured component 1 or directly via the material properties of the structured component 1 itself or by manufacturing it from more than one material eg is implemented by multi-component injection molding.

Another embodiment is in Figures 14a and 14b shown as a top view or as a sectional view, with an enlargement function 42 being introduced into the structured component 1 at a defined position above or below the chamber 2 or the channel system 3, which is designed, for example, in the form of a lens, in order to reach certain positions in the channel system 3 to be able to track better through the liquid and also to be able to read color reactions better as indicator reactions.

Another embodiment is in Figures 15a to 15c shown, with longer channel elements being introduced as flow limiters 43 into the course of the fluid in the channel system 3 in order to enable controlled liquid intake and discharge. The flow restrictors have a meandering shape and/or can be designed as channel tapers in order to control the flow of a fluid and/or to limit the speed

As in Figures 6a to 7b and Figure 9a and 15c shown, according to all embodiments, the chamber 2 can be connected to a plurality of channels or channel systems 3, which each open into at least one fluidic interface 5. The fluidic system can therefore have a plurality of fluidic interfaces 5 and the chamber 2 can have a plurality of channels or channel systems 3 branching off from them.

16 shows an embodiment of the chip in a view from above. The structured component 1 with a chamber 2 and the channel system 3 is shown. The channel system 3 connects the input 5.1. with chamber 2 and connects the chamber to the outlet 5.2.

A flow restrictor 43 is integrated into the channel system 3, which is designed in a meandering manner and/or can contain narrowing of the channel, with which the flow rate of the fluid can be controlled or reduced. A reservoir interface 17 with a liquid reservoir 16 is connected to the channel system 3 .

The input and output can be closed with a cap 14 which is attached to the chip by means of a tab 44 . preferably only one cap 14 is provided, which can be plugged alternately onto the input or output, in order to enable the chip to selectively absorb fluids when the input is open, ie without a cap 14, and the output is 5.2. is closed with the cap 14. In this way, a necessary negative pressure can be built up in order to take in a fluid via the fluidic interface 5.1 (input). After recording and corresponding analysis in the chip, the fluid should be released again. For this purpose, the cap 14 is then placed on the input and this is closed in a fluid-tight manner. Then the fluid via the output 5.2. be given. A switching between two functions of the chip can thus be made possible via the cap 14 .

In a further embodiment, it is possible to attach several caps 14 to the chip, for example to enable the chip to be transported or stored, with either the inside of the chip being protected from dirt and/or fluids present inside being able to leak out is prevented.

Reference list:

1

structured part/structured component

2

chamber

3

canal system/canal

4

component

5

fluidic interface

5.1

Entrance

5.2

Exit

6

flexible or movable area (on component 4)

7

flexible or movable area (on the structured component 1)

8th

second component

9

flexible or movable area (on the second component 8)

10

outlet (of the fluidic interface 5)

11, 12, 13

Printing elements, geometric elements, attachments

14

cap

16

fluid reservoir

17

Seat/Reservoir Interface

18

piercing elements

19

flap

20

detents

21

detection chamber

22

flares

24

membrane

25

ventilation channels

26

distribution system

27

diaphragm valve

28

rotary valve

28a

rotary valve seat

28b

rotary valve body

29

distribution channel

41

Capillary stop valves/channel tapers

42

magnifying device

43

flow restrictor

44

tab

Claims (15)

1. A microfluidic system, comprising:

   - a planar structured component (1) having a chamber (2) and a channel system (3),

   - at least one fluidic interface (5) via which the channel system (3) and the chamber (2) are fluidically connected to the outside,
   characterized by

   - a component (4) being arranged at a surface of the structured component (1), so that the chamber (2) and the channel system (3) are closed liquid-tightly at the surface of the structured component (1),

   - wherein the component (4) and/or the structured component (1) has a region (6) that is accessible from the outside, flexible, and movable and that is at least partially adjacent to the chamber (2),

   wherein the flexible and movable region (6) is formed to be pushed into the chamber (2) or to be moved out of the chamber (2) manually or by an operating device, so that liquids or gases can be taken in or discharged via the at least one fluidic interface (5) or can be moved within the microfluidic system, and

   wherein the fluidic interface (5) protrudes as a projection from a side surface of the structured component (1) to preset a predetermined volume of a drop of liquid to be discharged by means of an outlet geometry of the projection.
2. The microfluidic system according to claim 1, wherein the chamber (2) is connected via a further channel system (3) to a further fluidic interface (5), and wherein at least one of the fluidic interfaces (5) is closable with a cap (14) of the microfluidic system, and/or wherein a channel (3) leading to the chamber (2) has widenings (22), and/or wherein longer channel elements are incorporated as flow limiters (43) into a fluid path of the channel system (3) in order to enable a controlled liquid intake and liquid discharge, and/or wherein a defined movement of the flexible region (6, 7, 9) is achieved by means of geometric elements or attachments (11, 12, 13).
3. The microfluidic system according to claim 2, wherein the cap (14) has a flexible region that is formed to be pushed in or pulled out after attachment to thereby move the liquid in the channel system (3), and/or wherein one fluidic interface (5) is formed as an inlet (5.1) of the microfluidic system and one fluidic interface (5) is formed as an outlet (5.2) of the microfluidic system, and the inlet and outlet are arranged on one side of the system, wherein a cap (14) is fixed to the microfluidic system, preferably to the structured component (1), wherein the cap can be fitted either to the inlet (5.1) or to the outlet (5.2), thus enabling a liquid to be taken in at the inlet (5.1) or a liquid to be discharged at the outlet (5.2).
4. The microfluidic system according to any of claims 1 to 3, further containing a venting device for the chamber (2), wherein the venting device is arranged such that venting can take place via an additional channel (25) of the microfluidic system connected to the outside or a gas-permeable membrane (24) of the microfluidic system, and/or wherein the venting device is closable.
5. The microfluidic system according to any of claims 1 to 4, further containing an additional reagent reservoir (16) that is formed as a blister and/or wherein the chamber (2) is provided with reagents.
6. The microfluidic system according to claim 5, wherein the reagent reservoir (16) comprises:

   - a blister seat (17) having pointed elements (18), which are formed to pierce the blister (16) that is connected liquid-tightly and positioned above,

   - a flap (19) that can be pushed in using guide elements (20) in the blister seat (17), whereby a defined volume dosing is possible, wherein preferably a special configuration of the guide elements (20) enables a multi-stage volume dosing.
7. The microfluidic system according to any of claims 6, wherein a defined ejection of defined volumes is achieved by means of the flap (19), and/or wherein the flap (19) and the geometric elements or attachments (11, 12) formed as pushing elements are connected, combined, or coupled to one another on the flexible and movable region (6, 7, 9).
8. The microfluidic system according to any of claims 1 to 7, having a cavity (21) for optical readout and/or reaction, which preferably has different depths, and/or wherein the microfluidic system has a lateral flow strip (23), the filling of which is enabled by an operation of the chamber, wherein a venting membrane (24) and/or a venting channel (25) is coupled with the lateral flow strip (23).
9. The microfluidic system according to any of claims 1 to 8, having at least two chambers (2), wherein the at least two chambers (2) are directly connected to one another via a channel system (3), wherein the at least two chambers (2) are arranged in one or more planes, and/or attachments (11, 12, 13) on the flexible and movable component (6), which are located outside the chamber (2) or extend into the chamber (2).
10. The microfluidic system according to any of claims 1 to 9, further having movable elements inserted in the chamber (4) for mixing, which are preferably formed as balls or rods, and/or structural elements that are formed in the structured component (1) to enhance mixing, and/or a mixing device.
11. The microfluidic system according to any of claims 1 to 10, wherein the channel system (3) has alignment marks, which allow a volume indication, or wherein alignment marks, which allow a volume indication, are attached next to, below or above the channel system (3).
12. The microfluidic system according to any of claims 1 to 11, comprising at least one of:

    - a rotary valve (28), via which an intake or discharge of fluids is controllable,

    - membrane valves (27), via which an intake or discharge of fluids is controllable,

    - valves in the channel system (3) for intake of defined liquid volumes, wherein a valve function can be generated or enhanced by surface functionalization, wherein the valves (27, 28) enable a selective liquid discharge from individual fluidic interfaces (5),

    - a distribution system (26), which is connected to several fluidic interfaces (5), wherein the several fluidic interfaces (5) are selectively controllable, and/or a distribution system (26) comprising several channels, which opens into a corresponding number of fluidic interfaces (5) and enables a simultaneous intake and discharge of liquids, and/or wherein a uniform distribution of liquids in the distribution system (26) is supported by integrated passive valves (41).
13. The microfluidic system according to any of claims 1 to 12, further having a reservoir interface (17) by means of which a liquid reservoir (16) is connectable to the structured component (1), wherein the reservoir interface (17) is fluidically connected to the channel system (3) and/or to the chamber (2).
14. The microfluidic system according to any of claims 1 to 13, wherein dry reagents are incorporated in the channel system (3) of the structured component (1), wherein the dry reagents are taken in by the fluids flowing through and are mixed therewith, or wherein a reagent is provided at a defined position in or at the channel system (3) to colour liquid flowing over it so that a reaching of the position and thus a reaching of a predetermined volume or a defined dwell time is indicated.
15. The microfluidic system according to any of claims 1 to 14, wherein a magnifying device, which is formed as a lens, is arranged at at least one defined position above or below the channel system (3) or the chamber (2), so that a reaching of at least one specific position in the channel system (3) can be detected by liquid and/or colour reactions.

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