EP3793736A1 - Système fluidique pour recevoir, délivrer et déplacer des fluides, procédé de traitement de fluides dans un système fluidique - Google Patents

Système fluidique pour recevoir, délivrer et déplacer des fluides, procédé de traitement de fluides dans un système fluidique

Info

Publication number
EP3793736A1
EP3793736A1 EP19726597.8A EP19726597A EP3793736A1 EP 3793736 A1 EP3793736 A1 EP 3793736A1 EP 19726597 A EP19726597 A EP 19726597A EP 3793736 A1 EP3793736 A1 EP 3793736A1
Authority
EP
European Patent Office
Prior art keywords
fluidic
chamber
functional element
fluid
flexible
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19726597.8A
Other languages
German (de)
English (en)
Inventor
Claudia Gärtner
Richard Klemm
Christian Moche
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microfluidic Chipshop GmbH
Original Assignee
Microfluidic Chipshop GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microfluidic Chipshop GmbH filed Critical Microfluidic Chipshop GmbH
Publication of EP3793736A1 publication Critical patent/EP3793736A1/fr
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent

Definitions

  • the invention relates to a device for receiving, dispensing, diluting or moving liquids and for adding liquid components, for separating, filtering, fractionating, enriching liquids and / or their constituents, as well as modifying liquids and their constituents and for detection the ingredients of the liquids, which may also be referred to as a fluidic system, in particular a microfluidic system.
  • the device may also be referred to as a chip.
  • the invention relates to a method for processing fluids in a fluidic system.
  • the external pumps for the manipulation of Fab-on-a-Chip systems require a fluidic interface, for the use of which additional components are necessary and which, like all fluidic interfaces, involve the risk of feckage.
  • additional connected devices for carrying out the abovementioned sub-functions, in addition to a time delay, also present the risk of contamination and / or falsification of the analysis results.
  • Syringe pumps integrated directly into fluidic systems avoid a fluidic interface to the outside, but require another element, the plunger, to move liquids.
  • Diaphragm valves offer the advantage that they do without fluidic interface and without other components and only need a preformed well and a movable lid for actuation. These are designed so that they can be operated both pneumatically and mechanically. The operation of these diaphragm valves is usually carried out via an ent speaking operating device.
  • the recording and dispensing of liquids, the distribution to different reaction cavities, the movement of liquids and the addition of reaction components requires manual handling steps and / or a corresponding automation of these steps using large machines. This is done manually in the sample receiving and reagent supply by means of pipetting, the mixing and incubated, for example, by shaking titer plates and for the supply of reagents they are from appropriate storage containers to entneh men. Both the manual handling and the automated handling require a larger number of handling steps, additional equipment such as pipettes or pipetting machines as well as the Fagerjankeit the corresponding reagents. In microfluidic systems, handling is usually done via external pumps and the need for a device to control the system.
  • the present invention combines all handling steps including reagent pre-storage on a hand-operated component.
  • the object of the invention is to share the intake, delivery, dilution, transport and / or mixing of liquids and the separation, filtering, enrichment and / or fractionation of liquids and their components as well as the modification of liquids and their constituents and the Detection of the ingredients of the liquids both manually, ie to operate without further aids, as well as with appropriate devices.
  • a special feature of the system is that a multiple intake and delivery of liquids is possible and that desired volumes of the recorded and / or given off fluid can be precisely controlled. It is also an object to provide a method for the processing of fluids with such fluidic systems.
  • a fluidic system comprising a structured component with a chamber and a channel system, which are fluid-tightly sealed with a component, wherein the chamber via the channel system and a fluidic interface with the outside world is fluidically verbun the.
  • the component has a flexible or movable area which can be moved into the chamber area or beyond a level of the chamber.
  • the plane of the chamber is the upper boundary of the chamber on the side of the chamber, i. the underside of the chamber occluding component. Due to the movement of the flexible region, liquids or gases can be absorbed or discharged through the fluidic interface and / or moved in the fluidic system.
  • the movable area can be moved manually or with a corre sponding operating device.
  • One option is to push or pull up the flexible area to different positions. Particularly advantageous are the possibility of defined fluid delivery and recording by combining the chamber with a small Ka nalsystem, the Mehrfachaufhahme and delivery of liquids and the possibility of manual operation.
  • the fluidic system has an interface for a remplisstechniksreagenzi enreservoir.
  • the flexible or movable area is accessible from the outside.
  • the configuration of the component closing the structured component as a film wherein the film is at the same time the movable component due to its intrinsic flexibility.
  • the structured component may preferably be provided on its upper and lower sides with a respective covering component.
  • channel systems and / or parts thereof can be arranged on both sides of the structured component and sealed with the components.
  • the channel system can be easily inserted into the structured component on one or both sides from the respective surface and the channels on both sides can be connected by bores.
  • the component can be designed flexible, or it is designed as a foil, which then serves as a flexible region in the region of the chamber, since it is flexible in its basic property.
  • the volume can be defined by the corresponding outlet geometry of the fluidic interface, wherein this volume definition can be further influenced by a surface modification of the fluidic interface.
  • a further fluidic system comprising a structured compo nent with a chamber and a channel system which are sealed with another component, wherein the chamber is fluidly connected via the channel system and a fluidic interface with the outside world.
  • the flexible area is formed by the walls of the chamber.
  • a lateral pressing of the chamber allows the movement of the liquid or the compression effect can be enhanced by the flexible chamber walls.
  • yet another fluidic system comprising a struktu rators component or structured component and another component, the chamber and KanalSys tightly seals and the chamber is connected via the channel system and the fluid interface with the outside world.
  • the structured component is designed such that the Kammerbo is the flexible and can be pressed or expanded.
  • the floor can be made very flexible and a production by means of two-component injection molding is possible, so that a flexible component can be injected together with another component.
  • the basic material of the structured component can be sufficiently flexible to ensure the functionality of the component. An assembly of the flexible area in which the structured component is also possible.
  • the chamber may be verbun via another channel system with a fluidic interface, wherein one of the fluidic interfaces can be closed with a cap.
  • the closure with a cap further prevents fluid leakage at this point.
  • valves such as capillary stop valves, which act by changing the capillary diameter, the recording of defined volumes it allows.
  • a valve function and / or the function of existing geometrically acting valves is enhanced by a surface modification in the valve region, the geometrically generated valve function.
  • venting can take place in the event of fluid uptake by the second fluidic interface and, in addition, that fluid can be removed. take-off and delivery at different points.
  • the cap with a cap prevents further leakage of fluid at this point.
  • a corresponding positioning of the fluidic system, that the dispensing fluidic interface is inclined downwards in the liquid dispensing, is advantageous.
  • the fluidic system preferably contains a ventilation option for the chamber, which can take place via an additional channel communicating with the outside world or a gas-permeable membrane and this venting device can optionally be closed.
  • the fluidic system includes an inlet channel having a passive stop function, such as a capillary stop valve, a channel taper, or a corresponding surface modification, and either capillary action, which may be enhanced by surface modifications in the area to be filled, or one by the movable one Components caused change in the chamber volume takes a defined amount of liquid.
  • a passive stop function such as a capillary stop valve, a channel taper, or a corresponding surface modification
  • the fluidic system contains one or more reagents zienreservoire. These may be formed, for example, as blisters.
  • a plurality of fluids or dry reagents can be mixed with one another and the liquid reagents can be used for transporting absorbed or system-introduced dry reagents.
  • Dry reagents are preferably introduced into the structured component, which can be taken up by the fluids flowing through and mixed with them.
  • a reagent is presented at a defined location, which colors the fluid flowing over it and thus indicates the reaching of the position at which the reagent is present, and thus the achievement of a certain volume or a residence time.
  • an enlargement function is incorporated in the structured component, e.g. in the form of a Lin integrated into the structured component, in order to be able to better follow the attainment of certain positions in the channel system by the liquid and also to better read color reactions as indicator reactions.
  • longer channel elements are introduced as a flow restrictor in the fluid flow in order to allow a controlled fluid intake and delivery.
  • the reagent reservoir is formed as a blister.
  • the reagent reservoir has a blister seat which has pointed elements which pierce the overlying liquid-tightly connected blister.
  • This embodiment can be extended by a flap which, via guide elements in the blister seat, permits a defined insertion of the flap and thus a defined volume metering.
  • the Volumendosie tion can also be done in several stages by special design of the guide elements.
  • the liquid-tight closure of the fluidic interface is useful for fluid uptake, for example via a cap.
  • the cap may also be provided with a transport member, eg, a dome or plunger, which projects into the channel and thus conveys the fluid therein by volume displacement when the cap is placed on the fluidic interface.
  • the cap may also have a flexible Be rich, which can be pressed or pulled out after placement in order to move thus located in the channel and / or channel system liquid. When pushing the liquid is pushed further into the channel. As the flexible portion is withdrawn, liquid is conveyed from the channel toward the fluidic interface. This also allows small movements to be generated.
  • fluid volumes defined in this way can be dispensed from the blister, and that this can also be done manually with high precision.
  • an exact mixing ratio can be set.
  • the fluidic system has a long channel to the chamber. Particularly advantageous is this long channel, since it allows a speed of fluid intake can be adjusted and reagents can be introduced into the channel, which optimally resuspend by the long entrainment in the channel.
  • the long channel towards the chamber on additional expansions.
  • This embodiment is particularly advantageous because in the widening reagents can be pre-assembled and an improved mixing can be done by a different flow profile.
  • the fluidic system includes a cavity or Detekti onshunt for optical readout and / or reaction which may preferably have different depths.
  • a cavity or Detekti onshunt for optical readout and / or reaction which may preferably have different depths.
  • directly an optical detection can be done and in an embodiment of the detection chamber with several depths and the dynamic range can be enlarged ver.
  • the fluidic system includes a lateral flow strip, the filling of which is made possible by the operation of the chamber.
  • One embodiment leg holds a vent membrane, another a vent channel.
  • Particularly advantageous is the possibility of liquid intake, which can be operated manually, with the direct possi probability of readout on the lateral flow strip.
  • Just targeted ventilation options allow the combination of the achieved by the chamber vacuum-driven flow with the fol lowing fluid movement by the suction of the lateral flow strip.
  • a lateral flow strip serves to detect target molecules present in the fluid, whereby individual target molecules as well as different target molecules can be detected depending on the configuration of the lateral flow strip.
  • Wines of particular characteristics of the lateral flow strip is the integration of an array for the parallel detection of several target molecules.
  • the fluidic system includes more than one chamber, which are interconnected via a channel system and / or can be angeord net in one or more levels. It is particularly advantageous that a forwarding and a pushing back and forth and an active mixing on the change of the chamber volumes are made possible by the flexible elements.
  • the fluidic system includes attachments on the flexible components that are either external to the chamber or extend into the chamber.
  • attachments on the flexible components that are either external to the chamber or extend into the chamber.
  • the fluidic system has provided reagents in the chamber. It is particularly advantageous here that the chamber not only serves for the liquid movement, but the chamber volume directly for dissolving, reacting and mixing reagents can be used. Especially presented dry reagents allow a particularly advantageous use of the chamber here.
  • the cap for emptying the blister is directly connected to pressure elements for moving the flexible region, if necessary also realized in one piece.
  • mixing by movable elements introduced into the chamber is possible, such as spheres or rods, which may also be magnetic.
  • the mixing can be enhanced by structural elements in the structured component or be carried out entirely by them. It is particularly advantageous here that the simple structure of the system allows a particularly effective mixing in the chamber.
  • mixing takes place in the chamber by moving the fluidic system manually. It is particularly advantageous here that the simple design of the system allows manual use.
  • mixing takes place in the chamber through a device-side mixing mechanism. It is particularly advantageous here that efficient mixing can take place.
  • the channel systems themselves include alignment marks or adjacency marks are attached beside, below or above the channel system, which allow a volume indication. This marking is particularly advantageous, similar to a ruler, since it allows the user to read the recorded or dispensed volume and to stop or continue the recording or dispensing of volumes in order to set up, dispense or move defined volumes.
  • multiple liquid intake and / or dispensing is possible. It is particularly advantageous here that the fluidic system can be used for multiple picking and dispensing of liquids.
  • fluidic interfaces are provided on the structured component, which point in different directions, for example, perpendicular to the plane of the fluidic system or at a specific angle outgoing from the fluidic system.
  • Particularly advantageous here is that by a special geometry, the up and / or release of liquids in specially shaped surfaces or vessels can be done.
  • a plurality of fluidic interfaces are provided. This is particularly advantageous because then a delivery and absorption of liquids can take place at different locations simultaneously or sequentially.
  • the recording and delivery at several Stel len simultaneously or successively possible In combination with a distribution system, the recording and delivery at several Stel len simultaneously or successively possible.
  • a simultaneous delivery or absorption of liquids via the movement of the flexible elements can take place.
  • the intake and / or delivery of the fluids is controlled via diaphragm valves see. This is particularly advantageous because it allows an individual fluid intake and / or fluid delivery can be done at different fluidic interfaces by the movement of the flexible elements in the chamber.
  • a particular embodiment is the integration of passive valves in the individual distribution channels Ver a uniform filling and thus a uniform liquid transport and thus, for. to secure the delivery of equal volumes.
  • the intake and / or discharge of the fluids is controlled by rotary valves.
  • the rotary valves preferably have a rotary valve seat and a rotating the, the different parts of the channel system connecting rotary valve body with connecting channel on. This is particularly advantageous because it allows an individual fluid intake and / or fluid delivery at different fluidic interfaces by the movement of the flexible elements in the chamber.
  • the fluidic system is designed as a microfluidic system.
  • the structured component is preferably and essentially made of plastic.
  • the entire component may be e.g. be made as a plastic film. But it is also possible to apply a introduced into the other components flexible plastic such as silicone or TPE or a movable mechanical element made of any material to ver.
  • the abovementioned embodiments of the fluidic system have further fiction, according to at least one functional element, which can be realized for example by a filter, a Memb ran, a frit, or a functional paper or similar elements.
  • the one or more functional elements may be realized by one or more filters, membranes, frits, paper, or similar elements containing reagents or applied to the reagents.
  • Reagents may be applied to the structured component and / or the at least one component and / or the one or more functional elements and / or may contain these reagents, for example also in the form of arrays of the same or different avas.
  • a fluidic system comprising: a structured component having a chamber and a channel system, wherein the chamber and / or the Kanalysys system have at least one functional element, wherein at least the chamber is fluid-tightly sealed with a component and the channel system and at least one fluidic interface is fluidly connected to the outside world, the component having a flexible or movable area that can be moved into at least a portion of the chamber or beyond a level of the chamber, and liquids or liquid by movement of the flexible or movable portion Gases taken over the fluidic interface or istge ben and / or can be moved in the fluidic system, wherein the flexible or bewegli che range manually or with an operating device is movable and a pushing or moving up of the flexible or movable area is possible.
  • a fluidic system comprising: a structured component having a chamber and a channel system, wherein the chamber and / or the Kanalysys system have at least one functional element, and the at least one functional element is provided with reagents, wherein at least the Chamber is fluid-tightly sealed with a component and is fluidly connected via the channel system and at least one fluidic interface with the outside world, wherein the component has a flexible or movable area, we we least moved into a region of the chamber or beyond a plane of the chamber can be to receive or deliver fluids or Ga se via the fluidic interface by movement of the flexible or movable area and / or move in the fluidic sys tem, wherein the flexible or movable area by hand or with a loading operating device is movable and an impressions or Moving up the flexible o the movable range is possible.
  • a fluidic system comprising: a structured component having a chamber and a channel system, wherein reagents are applied to the structured component and / or on the component closing it and / or on the at least functional element, wherein at least the chamber is fluid-tightly sealed with a component and is fluidically connected to the outside world via the channel system and at least one fluidic interface, the component having a flexible or movable region extending at least into a region of the chamber or beyond a plane of the chamber can be moved, and by a movement of the flexible or movable area liquids or gases can be absorbed or discharged through the fluidic interface and / or moved in the fluidic system, wherein the flexible or movable portion is movable by hand or with an operating device and an impressions or Upward movement of the flexible or movable area is possible.
  • the functional element is realized by a filter, a membrane, a frit and / or a functional paper. All these examples of the functional elements are at least partially passable for fluids, that is porous. They may be membranes and / or filters for size exclusion such as laser-structured membranes (track etch) with well-defined pore size, silicon screens, filter paper with a coarse-meshed net. Functional elements which use the size exclusion and / or connection to the surface of the functional element are different elements, such as e.g.
  • porous three-dimensional structures such as frits, silicon membranes, silica membranes, three-dimensionally aggregated particles, filter mats of various materials, silica mats, PET filters, thin-layer chromatography material or plasma / serum generation membranes, to name but a few examples. All of these functional elements can additionally be provided with reagents in order to implement a specific binding of target molecules to these functional elements and to realize a targeted detachment of the target molecules from functional elements.
  • the fluidic interface there is a recording of a fluid or fluid via the fluidic interface, wherein the liquid is passed through or over the functional element.
  • the fluid can then be released again by pressurizing the chamber of the thumb pump.
  • the absorption of the fluid via capillary forces or a surface tension which is caused by the surfaces of the channel system and / or the chamber and / or the fluidi's interface.
  • the recording of the fluid via the operation of the chamber of Dau menpumpe takes place.
  • the fluidic system comprises at least one valve which is arranged in the channel system, wherein the integration of the valve a defined volume can be accommodated men.
  • the at least one functional element on a Saugglantation whereby the fluid intake is driven.
  • the suction function can be caused by a hygroscopic property of the materials of the functional element.
  • the entrance can be closed with a cap.
  • the input and output can be closed with a cap.
  • the at least one functional element causes in a flow of blood that only plasma or serum passes through the functional element and other blood components are held back by the functional element, wherein a discharge of the recovered plasma or serum can be done via the fluid outlet.
  • two functional elements are connected in series. That is, in the channel system, two functional elements with the same or different properties are arranged.
  • the two functional elements are connected in series, wherein initially the one functional element is flowed through and the liquid passes to the chamber of the thumb pump. Then, the fluid inlet is closed with a cap, wherein the liquid passes to the second functional element by a movement of the flexible component and is then discharged via the fluid outlet. That the first functional element is arranged in the flow direction from the entrance to the exit in front of the chamber in the channel system and the second functional element is arranged in the flow direction Rich behind the chamber or between the chamber and fluid outlet.
  • two functional elements are connected in series, wherein the first ei ne functional element is flowed through, and then the liquid flowed through penetrates through the second functional element and is discharged by a movement of the movable member via the fluid idausgang. After receiving the liquid via the fluid inlet of this is closed, for example, with a cap.
  • the at least one functional element is used to generate plasma or
  • the first functional element is for the production of plasma or serum, where the second functional element removes hemolyzed red blood cells.
  • a liquid reservoir is connected to the structured component, where at by the liquid discharge from the liquid reservoir, a dilution of the fluid takes place within the channel system.
  • a liquid reservoir is connected to the structured component, where a dilution of the fluid takes place within the channel system via the liquid discharge from the liquid reservoir, wherein a defined volume can be dispensed from the liquid reservoir to a defined volume of the liquid received.
  • a liquid reservoir is connected to the structured component, where at the liquid discharge from the liquid reservoir, a dilution takes place and wherein a defined volume from the liquid reservoir is added to a defined volume of aufgenomme nen liquid that has already been passed through the functional element. That is, in the flow direction after the fluid inlet, the first functional element is arranged to perform a first treatment of the liquid absorbed, then a remplisstechniksre reservoir is connected to the channel system to the already treated by the first functional element th liquid a defined amount of existing in the liquid reservoir Add liquid.
  • a liquid can be taken up via the fluidic interface, mixed with liquid in a reaction cavity and then guided over and / or through at least one functional element, wherein target molecules of the liquid remain on the functional element and the target molecules by a Liquid can be detached from a liquid reservoir and discharged via the fluidic interface (output, 5.2).
  • At least one further liquid reservoir is fluidically connected to the functional element, whereby the functional element can be flowed through by different liquids in order to free the functional element of unwanted constituents or to displace the dissolved target molecules.
  • the detachment of target molecules from the at least one functional element is preferably effected by a temperature change.
  • the absorbed liquid is first passed through a first functional element, wherein particles are retained on the first functional element. These particles are then broken down into smaller particles and fed to the next functional element, where part of the smaller particles produced is retained by the functional element and can be released again, the target particles being found in a different fraction of the eluate than the undesired ones to be separated Components or other target components.
  • a cleaning wherein unwanted particles are removed from the functional element.
  • the particles are cells, wherein the particle size reduction step is the cell fysis.
  • biological components such as nucleic acids, proteins, metabolites and / or antibodies are extracted, concentrated and / or purified.
  • the resulting target component is subsequently processed via integrated reagents such as arrays, i. an array of capture molecules, guided to attach the target molecules / target particles to the Arraymoleküle and subsequently detected.
  • integrated reagents such as arrays, i. an array of capture molecules
  • the resulting target component is detected with the system and / or identi fied, further preferably detected quantitatively.
  • one or more functional elements are arranged in parallel. That is, the channel system has a plurality of parallel strands in each of which one or more functional elements are arranged.
  • the reagents applied to the structured component, the component, and / or the functional element, upon contact with liquid exhibit a color change indicating a fill indicator.
  • these reagents are on, in or on the functional element.
  • the fluidic system is also referred to as a thumb pump, because the flexible component is particularly easy to operate with the thumb.
  • FIGS. 1 to 16 show basic variants of the thumb pump, which according to the invention have been extended in their functionality by further elements, which are shown in FIGS. 17 to 25.
  • Fig. La to lc a fluidic system according to an embodiment.
  • Fig. 2 is a fluidic system according to an alternative embodiment.
  • Fig. 3 is a fluidic system according to another alternative embodiment. 4 shows fluidic interfaces of a fluidic system according to embodiments.
  • FIGS. 7a and 7b show a fluidic system according to yet another embodiment.
  • FIG. 11 shows a fluidic system according to another embodiment.
  • FIGS. 12 a to 12 d illustrate a fluidic system with a distributor system according to embodiments.
  • Fig. 13 shows a fluidic system according to another embodiment.
  • FIGS. 14 a, 14 b show a fluidic system with a magnification device according to an embodiment.
  • FIGS. 15A-15C show a fluidic system with flow restrictors according to embodiments
  • FIG. 16 shows an embodiment of the chip with cap in a view from above FIG. 17A-D an embodiment with functional element such as membrane, filter, frit, paper or similar element, FIG.
  • FIGS. 18 a - c show an embodiment with integrated reagents, for example as an array, FIGS. 18 a and 18c as a cross section, FIG.
  • Fig. L9a, 19b, 19c an embodiment with the chamber upstream and downstream
  • Fig. L9a filter, membrane, frit, paper or similar element
  • Fig. 19b as a cross section
  • Fig. L9c as a cross section with cap
  • FIG. 20a, 20b, 20c show an embodiment with two functional elements as a plan view (FIG. 20a) and a cross section (FIG. 20b, c), FIG.
  • 2la, 2lb, 2lc an embodiment with two functional elements as a plan view (Fig. 2la) and cross-section (Fig. 2lb, c), which has a liquid reservoir
  • Fig. 22a, 22b, 22c an embodiment with two functional elements as a plan view ( 22a) and cross-section (FIGS. 22b, 22c)
  • a reagent reservoir and capillaries that can be closed by capillaries
  • Fig. 23a, 23b, 23c an embodiment with a functional element as a plan view (Fig. 23a) and
  • FIGS. 23b, 23c Cross section (FIGS. 23b, 23c), a venting membrane and fluidic interfaces that can be closed by means of caps,
  • Fig. 24a, 24b, 24c an embodiment with a functional element as a plan view (Fig. 24a) and
  • Fig. 25a, 25b, 25c an embodiment with two functional elements as a plan view (Fig. 25a) and cross-section at the level of liquid reservoirs (Fig. 25b) and on a cross-section at the level of the first functional element and the chamber (Fig. 25c), and three closable via caps fluidic interfaces and, for example, three fluid reservoirs.
  • 26a, 26b show an embodiment with a functional element as a plan view (FIG. 26a) and as a cross-section (FIG. 26b), a fluidic interface that can be closed by a cap, a vent opening provided with a gas-permeable membrane 24 as a further fluidic interface, and FIG a Fateral Flow Strei fen 23.
  • FIG. 27a, 27b show an embodiment with two functional elements as a top view (FIG. 27a) and a cross section (FIG. 27b), of a fluidic sealable via a cap Interfaces, provided with a gas-permeable membrane 24 vent opening as a further fluidic interface, a lateral flow strip 23rd
  • FIG. 28a, 28b, 28c show an embodiment with two functional elements as a plan view (FIG. 28a) and cross section (FIG. 28b, c) of a fluidic interface which can be closed by a cap, a vent opening provided with a gas-permeable membrane 24 as a further fluidic interface , a lateral flow strip 23 and a reagent reservoir 16 opening at any point into the channel system 3 via a feed channel and a cavity serving as a waste reservoir 49 as a component of the channel system 3.
  • the present invention describes a fluidic system having a chamber which has a flexible or movable part, mostly the bottom and / or lid, in special embodiments, but also movable walls, which, by lifting or pushing down, picking up , Moving, diluting or mixing of liquids or gases, which are connected via at least one channel or opening to the chamber.
  • An inven tion proper extension is achieved either by additional elements such as filters, membranes, frits or similar elements and / or integrated reagents, which may be arranged for example in the form of an array of the same or different reagents. This can enable separation, filtering, fractionation, enrichment of liquids and their constituents as well as the modification of liquids and their constituents and the detection of the ingredients of the liquids.
  • the individual use and the combination of the additional elements can be arbitrary.
  • the chamber and the movable part are designed so that a predetermined, but adjustable volume of the chamber is displaced by a movement of the movable part from its initial position.
  • predetermined volumes in the return of the movable part can be added to a different position or in the starting position in the chamber or give ist.
  • the volume is predetermined by the properties of the fluidic system or can be adjustable by the configuration of the fluidic system according to the invention.
  • FIGS. 1 a to 1 c show an embodiment of the fluidic system.
  • 1 a and 1c show a top view of the fluidic system
  • FIG. 1 b shows a cross-sectional view of the fluidic system.
  • the fluidic system has a structured component 1 with a chamber 2, wherein the chamber 2 is connected to a channel system 3.
  • the structured component 1 is essentially flat, planar and / or plate-like.
  • the structured component 1 has a first main side and a second main side which are parallel availabilitylie conditions.
  • the chamber 2 and the channel system 3 are formed on the first main page and / or in the Oberflä surface of the structured component 1.
  • the chamber 2 and the channel system 3 on the main side are embedded in the surface of the structured component 1.
  • the chamber 2 and the channel system 3 thus represent a depression on the surface of the structured component 1.
  • the first main page is for example an upper side
  • the second main page is, for example, an underside of the structured component 1, wherein the orientation of Obersei te and bottom is arbitrary and turning the structured component to the top for and vice versa.
  • the structured component may be formed, for example cuboid.
  • the structured component 1 may also be formed slices shaped.
  • the structured component may take any form as long as it is substantially flat.
  • the structured component 1 can be designed, for example, as a platform.
  • the structured component 1 can be designed flat.
  • the chamber 2 and / or the channel system 3 thus has an upper side which corresponds to the upper side of the structured component 1.
  • An underside of the chamber 2 and / or of the channel system 3 is formed within the structured component 1.
  • the underside of the chamber 2 can also be referred to as a chamber bottom 7. Between the top of the chamber 2 and the lower side of the interior of the chamber 2 is formed, in turn, that the top and bottom can be according to the consideration both bottom and top.
  • the chamber 2 and / or the channel system 3 may be formed as a depression in the structured component 1, e.g. be formed on the top or the bottom of the structured component 1.
  • the chamber 2 and the channel system 3 can be thoroughlybil Det as different deep wells, which in turn is that the top and bottom can be according to the consideration both bottom and top.
  • the chamber 2 and / or the channel system 3 are connected via one or more fluidic interface 5 fluidly connected to the outside world.
  • the fluidic interface 5 is an opening of the channel system 3, preferably on a side surface of the structured component 1.
  • the opening of the fluidic interface 5 may also be arranged on an upper side or lower side of the fluidic system.
  • the fluidic interface 5 can protrude as a projection from a side surface of the structured component 1.
  • the fluidic system can have a plurality of fluidic interfaces 5 which are each connected to the channel system 3.
  • the fluidic interfaces 5 can be arranged on different surfaces of the structured component 1, e.g. the top, bottom, or sides preferably surfaces on opposite side surfaces.
  • the outlets of the fluidic interfaces 5 may point in different directions. Thus, they may have different orientations with respect to the center of the structured component 1.
  • a component 4 closes the channel system 3 and the chamber 2 liquid and, if necessary, gas-tight, so that the supply and delivery of liquids and gases can be made only via the one or more fluidic interfaces 5.
  • the component 4 is arranged on the surface of the structured component 1 such that it closes the chamber 2 and the channel system 3 at the top of the structured component 1.
  • the component 4 may e.g. be glued to the structured component 1, bonded, pressed, pressed or welded to the structured component 1 or be sealed by means of sealing elements such as sealing Weichkompo components.
  • the component 4 thus serves as a lid to close the structured component 1.
  • the interior of the chamber 2 is bounded at the top of the chamber 2 by the underside of the component 4.
  • the component 4 can essentially consist of a transparent Material be designed to observe the flow of fluids in the channel system 3 and / or in the chamber 2.
  • the chamber 2 may have a substantially flat oval, rectangular or round shape.
  • the chamber 2 and / or the interior or the volume of the chamber 2 is thus defined on the one hand by the structured component 1 and on the other hand by the component 4.
  • either the entire component 4 is flexible or the component 4 has a flexible or movable region 6.
  • the flexible portion 6 of the component 4 is disposed above half of the chamber 2 as a direct part of the component 4.
  • the flexible or movable region 6 may be formed as a further component of the fluidic system.
  • the flexible and / or movable region 6 of the component 4 should be arranged at least on a region of the chamber 2 and / or on an outer side of the chamber 2.
  • the component 4 may e.g. be formed as a film or strip and may be made of plastic or metal.
  • FIGS. 2 and 3 Alternative embodiments of the fluidic system are shown in FIGS. 2 and 3.
  • the structured component 1 has a flexible region 7 below the chamber 2.
  • the flexible region 7 below the chamber 2.
  • the flexible region 7 can be realized either by attaching or attaching the component for the flexible region 7 in or on the structured component 1.
  • the flexible region 7 can also be embodied as a partial material property of the structured component 1 itself or by the production of more than one material, e.g. be implemented by multi-component injection molding.
  • the structured component 1 is closed with the component 4 and moreover with a further component 8, wherein one or both components 4 and 8 can have a flexible or movable region 9.
  • the component 4 is arranged on the upper side of the structured component 1. That is, the top of the chamber 2 is closed with the component 4.
  • the further component 8 is arranged. That is, the underside of the chamber 2, so the chamber bottom, with the other component
  • a flexible region 9 is shown in the further component 8. Again, the top and bottom can be both bottom and top, as viewed.
  • the structured component 1 is preferably formed with a cover film which has sufficient flexibility for pressing in and lifting above and / or below the chamber 2.
  • the chamber 2 is designed such that the and / or the flexible regions 6, 7, 9 do not fill the entire chamber 2 when pushed into the chamber 2.
  • the flexible region 6, 7, 9 does not terminate flush with the chamber bottom when the flexible region 6, 7, 9 is pressed into the chamber 2. That is, liquid or gas that is in the chamber 2 and is not completely displaced from the chamber 2 by pushing the flexible portion 6, 7, 9.
  • a tight closure of the flexible regions 6, 7, 9 with the chamber bottom or the subsequent channel systems 3 is not necessary for the functionality, but the movement of the flexible regions 6, 7, 9 causes the movement of the medium.
  • the flexible area 6 is manually and / or dog-like, e.g. pressed down with a user's finger, or by means of an operating device from the starting position.
  • the flexible portion 6 is pressed from its initial position by pressure in the chamber 2. That is, the flexible portion 6 is pushed into the interior of the chamber 2 via the upper side.
  • the fluidic interface 5 is immersed in a liquid.
  • the flexible region 6 either automatically or partially returns to the starting position due to the material property of the flexible region 6, or is caused by movement of the operating device, e.g.
  • the interior of the chamber 2 is increased again by moving the flexible area 6 back into the starting position. Due to the increase in volume of the interior creates a negative pressure in the chamber 2 and / or in the adjacent channel system 3, which communicates via the fluidic interface with the liquid. That is, the negative pressure draws liquid into the fluidic system. In other words, a part of the liquid is initially drawn into the channel system 3 by the suppression and then also into the chamber 2 if the pressure is sufficiently great. Liquid is thus absorbed into the fluidic system.
  • the volume of the liquid and / or the Positionie tion of the liquid in the channel system 3 and / or in the chamber 2 of the fluidic system By adjusting the displaced by the depression of the flexible portion 6 volume of the inner space of the chamber 2 and / or by a defined return of the flexible portion 6 in the starting position, the volume of the liquid and / or the Positionie tion of the liquid in the channel system 3 and / or in the chamber 2 of the fluidic system.
  • Mixing Liquids Mixing of the collected liquid is accomplished by first drawing liquid into the chamber 2, i. Liquid is first added to the fluidic system. Subsequently, either the flexible member 6 is moved, or the flu idische system itself is moved. The movement of the fluidic system takes place, for example, by a multiple tilting of the fluidic system. In this case, a rapid shaking should be the avoided to avoid that arise Fuftblasen in the liquid absorbed. As a result of the movement, the fluids in the fluidic system are mixed with one another.
  • the discharge of liquids from the fluidic system takes place in that the flexible component 6 and / or the flexible components are pressed into the chamber 2.
  • the volume or the interior of the chamber 2 which is limited by the flexible member, is reduced by the pushing of the flexible member.
  • the liquid speed which is located either in the chamber 2 or in the channel system 3, according to the displaced by the movement of the flexible portion 6, ie by pushing the flexible portion 6 into the chamber 2, volume discharged from the fluidic system. That is, the displaced liquid is discharged from the chamber 2 via the channel system 3 through the fluidic interface 5 from.
  • the volume of the discharged liquid may correspond to the volume of the interior of the Kam mer 2, by which the chamber 2 is reduced by the pushing of the flexible portion 6.
  • multiple volumes of liquid can be dispensed.
  • the multiple delivery can take place in that the flexible region 6, 7, 9 is gradually pushed further into the chamber 2 and / or the interior of the chamber 2.
  • the multiple donation can also take place in that the flexible region 6, 7, 9 is first pressed into the chamber 2 once and that the flexible region 6, 7, 9 then either moves out of the chamber 2 independently or as described above using an operating device the Kam mer 2 is moved out.
  • the outward movement at least a portion of the liquid flows back into the channel system 3 connected to the chamber 2.
  • the outward movement is followed by repeated impressions of the flexible area 6, 7, 9 into the second liquid dispensing chamber 2.
  • a pumping movement and / or a pumping functionality is performed by the repeated and alternate impressions in the chamber 2 and moving out of the chamber 2 of the flexible portion 6, 7, 9. This leads to a repeated and changing liquid absorption and liquid delivery.
  • the fluidic interface 5 for sampling is closed by a cap 14.
  • the cap 14 may also have integrated projections before, which protrude into the channel system 3, when the cap on the fluidic interface 5 is placed. As a result, liquid can be displaced in the channel system 3 and pressed into the further channel system 3.
  • a fluidic interface 5 is designed as input 5.1 and another fluidic interface 5 as output 5.2 of the fluidic system.
  • the entrance 5.1. and output 5.2 are preferably formed on the structured components 1.
  • the two fluidic interfaces 5.1 and 5.2 are formed on one side, preferably on one end face or narrow side of the chip (fluidic system). That the input and the output are located on one side of the system. This makes it possible to close the input and output with a cap 14, which is also referred to as a jumper.
  • the cap 14 is preferably attached to the fluidic system, preferably to the structured component 1.
  • One or more caps 14 may be attached.
  • only one cap 14 is provided, which can be plugged either to the input 5.1 or output 5.2. This can then selectively picking up a liq fluid at the entrance 5.1. or dispensing liquid at the exit 5.2.
  • the one or more caps 14 are attached via a tab 44 on the chip (fluidic system).
  • the flexible region 6 can thus be pressed into the chamber 2, more precisely into the interior of the chamber 2 by pressure from the outside due to its flexibility under ei ne level defined by the top of the structured component 1.
  • the flexible region 6 may be formed by external pulling, e.g. be pulled out of the interior of the chamber 2 by means of negative pressure or an attached device. That is, it may be moved beyond the plane defined by the top of the structured component 1.
  • a recording, dilution delivery, dosage and / or transport of liquids is possible. Liquid that has been taken up in the fluidic system can be see systems are transported and stored. Multiple intake and multiple delivery of liquids is possible. Mixing liquids is possible.
  • the fluidic system can be used by the design of the chamber 2 and the flexible regions 6, 7, 9 as a pipette with functions of fluid intake, fluid delivery and the multiple Aufhehmen and dispensing fluids.
  • the operation can be done completely manually without further aids or by means of an operating device.
  • FIG. 4 shows embodiments of the fluidic interface 5.
  • the embodiments of the fluidic interface 5 according to FIG. 4 differ in terms of their geometry. More specifically, the illustrated embodiments of the fluidic interface 5 each have an outlet 10, wherein the shape of the outlet 10 differs in the embodiments shown.
  • the geometry of the outlet 10 of the fluidic interface 5 is also decisive for the volume of liquid dispensed.
  • the flexible region 6, 7, 9 hineinge presses into the chamber 2, so that forms a drop of the liquid at the outlet 10 of the fluidic interface 5.
  • the flexible portion 6, 7, 9 is further pressed into the chamber 2 as long as the liquid droplets from the outlet 10 tears off. Subsequently, the pushing in of the flexible region 6, 7, 9 and / or the dispensing of liquid can be ended. Alternatively, the flexible portion 6, 7, 9 can be further pressed into the chamber 2, to generate a further drop of liquid erzeu.
  • FIGS 5 a to 5f show pressure elements of the flexible regions according to various Ausry tion forms.
  • the flexible regions 6, 7, 9 may have pressure elements 11, 12, 13 in order to extend a defined depression of the flexible regions 6, 7, 9 into the chamber 2 and / or a defined fro and / or moving out of the flexible regions 6, 7 To allow 9 out of the chamber 2.
  • pressure elements 11, 12, 13 may be arranged on the flexible regions 6, 7, 9, and / or applied in order to prevent differences due to a person-dependent force or finger size even with manual actuation and / or manual operation.
  • the printing elements 11, 12, 13 can be operated either manually and / or manually, eg with a finger, or by an operating device.
  • the pressure elements 11, 12, 13 may be applied to the flexible region 6 materials.
  • the pressure elements 11 may be formed as a silicone hemisphere, as shown for example in Figures 5a and 5b.
  • the pressure elements 12 can be manufactured directly with the flexible region 6, for example by multi-component injection molding, as shown in FIGS. 5b and 5c.
  • a defined press A can also be adjusted via pressure elements 13 which are arranged as upstanding elements in the structured component, as shown in Figures 5e and 5f.
  • FIGS. 5a, 5c and 5e respectively show the initial state of the flexible region 6, 7, 9, ie the state when no force and / or no pressure is exerted on the flexible region 6, 7, 9.
  • FIGS. 5b, 5d and 5f respectively show a position before a fluid intake and / or during fluid delivery, ie a position of the flexible region 6, 7, 9 when it is pressed into the chamber 2.
  • FIGS. 6a and 6b show further embodiments of the fluidic system in which two separate fluidic interfaces 5 are arranged.
  • the fluidic interfaces 5 are arranged on different, more specifically opposite, side surfaces of the structured component 1 and protrude from the respective side surfaces.
  • the liquid absorption can take place by one of the two fluidic interfaces 5, and the liquid removal can be carried out by the other of the two fluidic interfaces 5.
  • the fluidic interfaces 5 can also be closed by one or more caps 14 in order to avoid contamination or leakage of fluid from the fluidic interface 5.
  • Fig. 6b only one cap 14 is shown.
  • the caps 14 the fluid received in the fluidic system can be transported and stored very safe and easy.
  • the caps 14 can be placed on the fluidic interface 5, more precisely, on the openings formed by the fluidic interface 5, on the respective side surface of the structured component 1, and terminate the fluidic interfaces 5 in a fluid-tight manner.
  • the fluidic system can have a liquid reservoir 16.
  • the liquid reservoir 16 is connected via a channel to the channel system 3 and / or to the chamber 2.
  • the channel may be part of the channel system 3.
  • the diesstechniksreser rium 16 may be formed for example by one or more so-called blisters, ie, liquid-filled, for example, by a piercing opening compartments, which are mounted liquid-tight on the fluidic system.
  • a pesstechniksaufhahme from the blister is achieved by expressing the blister itself, ie with positive pressure or by a above-described be writing the flexible portion 6 and a moving out of the flexible portion 6 from the chamber 2, wherein by the resulting negative pressure in the chamber.
  • the mixing can be facilitated by the placement of the cap 14 on the fluidic interface 5 and / or reinforced, since with attached cap 14 of the resulting by moving the flexible portion 6 suppression of the liquid speed in the liquid reservoir 16 acts.
  • the liquid reservoir 16 may also be referred to as a reagent reservoir or liquid reagent reservoir, and may include any type of liquid. In a particular embodiment, these reagent reservoirs may also include gases.
  • a mixing of the liquids can be effected by a movement of the fluidic system, a movement of the flexible region 6, 7, 9 or introduced mixing elements.
  • the mixing elements for example balls made of silicone, hard plastic balls, metallic components or other particles, can be done by the manual movement of the fluidic system.
  • the mixing may be accomplished by mixing elements of magnetic materials moved through an outside mixing device.
  • Figures 7a and 7b show an embodiment of the fluidic system combining two types of fluid intake.
  • the sample intake takes place by moving the flexible area 6, 7, 8 of the chamber 2 into the chamber 2 and moving out the flexible area, as described above.
  • an independent fluid intake into the fluidic system via a passive filling, i. by capillary forces of the channel system 3 or special surface properties of the channel system at the fluidic interface 5 done.
  • the suction effect produced by the suppression and / or by the capillary forces, and thus the filling speed can be achieved by surface modification, e.g. a hydrophilization of the channel surface of the channel system 3, reinforced and / or accelerated.
  • the volume of the liquid taken up can be defined.
  • a defined amount of liquid will be received, with a cap 14 prevents the discharge of the liquid reservoir 16, the leakage of the liquid.
  • FIGS. 8a to 8e show a fluid reservoir ejection mechanism 16 in accordance with embodiments.
  • the Ausdrückmechanismus can be formed, for example, as a flap 19, wherein the engagement of the flap 19, as shown in Fig. 8d, the introduction of a defined amount of liquid from the liquid reservoir 16 into the channel system 3 of the fluidic system be, with a defined mixing ratio of the liquid is reached from the liquid reservoir 16 with the liquid (sample) received in the fluidic system.
  • 8d shows a state in which the flap 19 presses the liquid reservoir (blister) 16 onto the fluidic interface 5 of the channel of the channel system 3. This principle is erber terbar on more liquid reservoirs 16 and thus used for multiple blends.
  • Fig. 8a shows a squeeze-out mechanism with seat 17, which may be formed as a blister seat and via piercing elements 18, e.g. small tips, features.
  • the piercing elements 18 are shown only in Fig. 8a.
  • Fig. 8b shows an embodiment of a Ausdrückmechanismus, wherein the seat 17 Rastna sen 20 and the flap 19 has a hinge-like manner on the locking lugs 20 of the seat 17 is mounted on it.
  • the liquid reservoir 16 is arranged on the flap 16.
  • the ejection mechanism shown in Fig. 8b may also have through-hole elements 18 (not shown) beyond.
  • one of the latching lugs 20 serves as a hinge and another of the latching lugs 20 as a latching surface and / or support surface for the cap 19, so as to limit a rotation of the cap 19.
  • the liquid reservoir 16 is pierced and the liquid from the liquid reservoir 16 can be received in the channel system 3 of the fluidic system.
  • the seat 17 may also be referred to as a reservoir interface.
  • FIG. 8 c shows an embodiment of the ejection mechanism, in which the liquid reservoir 16 is arranged on the surface of the structured component 1.
  • the flap 19 can have a bulge and / or projection, as shown in Fig. 8d, so that the liquid reservoir 16 is expressed by the projection when the flap 19 is closed.
  • Fig. 8d shows the depressed push-out mechanism, in this case the flap 19.
  • FIG. 8e is a plan view of a seated expressing mechanism 17 according to an embodiment.
  • FIG. 8e is a plan view of a seated expressing mechanism 17 according to an embodiment.
  • Figures 9a and 9b show a fluidic system with a long channel system 3.
  • the channel system 3 meanders between the fluidic interface 5 and the chamber 2, whereby the length of the channel system 3 is increased. As a result, an indwelling stretch is created for the liquid taken up in the fluidic system.
  • the residence section can be filled with reagents, for example dried reagents.
  • a long channel system 3 can be formed.
  • the channel system 3 may further comprise widenings 22 for better mixing, as shown in Fig. 9a, or another passive mixing element.
  • the widenings 22 can, as shown, be designed as elongated and / or in the flow direction in the channel system 3.
  • liquid and / or reagents can be introduced, which mix with the liquid received in the channel system 3 and / or the fluidic system and / or with the liquid dispensed by the fluidic system.
  • the channel system 3 may also have an optical detection chamber or reaction chamber 21 as shown in Fig. 9b.
  • An embodiment of the detection chamber 21 at different depths is particularly advantageous in order to expand the dynamic range of the measurement.
  • the detection chamber 21 may be inserted at different depths into the structured component 1, so that it may be e.g. has stepwise different depths Detektionsschöden.
  • a further option of the extension is the introduction of a lateral flow strip 23, as shown in FIGS. 10a to 10c, which can be defined by means of the pumping function of the fluidic system and / or, after wetting with liquid, self-inflates via capillary forces.
  • a combination of a filling by the pumping action of the chamber 2 in the above-described manual operation and / or by means of an operating device and the suction effect of the late-flow strip can take place.
  • the lateral flow strip is introduced into and / or introduced into a further chamber, which is likewise connected to the channel system 3.
  • vent channels 25 or gas-permeable and liquid-tight Membra nen 24, which are each connected to the channel system 3 and / or the chamber of the lateral flow strip ver, for operating the system is particularly advantageous. This is illustrated, for example, for the gas-permeable and liquid-tight membranes 24 in FIG. 10b and for venting channels 25 in FIG. 10c.
  • FIG. 11 shows a fluidic system according to yet another embodiment.
  • the structured component 1 has two chambers 2, which are embedded in the top of the structured component 1 Tur.
  • the two chambers 2 are directly connected to each other via a first channel system 3a and / or a channel 3a.
  • a respective second channel system 3b and / or a channel 3b the two chambers 2 are also each connected to a fluidic interface 5.
  • This embodiment of the fluidic system can also be referred to as a combined chamber system.
  • the use of combined Kammersys A further embodiment of the fluidic system can then be used simultaneously as a mixing, reaction, pumping and / or metering unit.
  • Figures l2a to l2d show embodiments of the fluidic system with Verteilersyste men 26.
  • a chamber 2 at one end to a manifold system 26 is connected.
  • the distribution system 26 may be part of the channel system 3.
  • the Verteilersys system 26 has one or more channels, which drove away from the chamber 2 and thereby ver branches.
  • the ends of the respective branched channels of the manifold system 26 are each connected to egg ner fluidic interface 5.
  • one channel in each case leads away from the chamber 2 and branches into in each case 4 channels, which are each connected to a fluidic interface.
  • the Verteilersys systems allow a simultaneous or sequentially switched fluid intake and / or - by the movement of the flexible portion 6, 7, 9 and the associated change in the Kammervo lumens.
  • FIGS. 12 a and 12 b show a fluidic system with a distributor system 26, wherein the channel leading away from the chamber 2 branches stepwise, namely initially into two further channels. The two further channels then each branch once again into two further channels, so that the channel leading away from the chamber 2 branches off into a total of four channels, which open into the respective plurality of fluidic interfaces 5.
  • all fluidic interfaces 5 are controlled and / or activated simultaneously by a movement of the flexible region 6, 7, 9.
  • the branched channels of the distribution system 26 may have membrane valves 27.
  • the use of diaphragm valves 27 requires impressions of the diaphragm valves 27 and a liquid-tight closure of the same, to close the respective channels individually or together and thus to be able to implement via the fluidic interfaces 5, the fluid intake and / or delivery can.
  • the liquid flow within the respective channels can be controlled in a targeted and defined manner. That is, the individual fluidic interfaces 5 can be controlled and / or controlled by means of the diaphragm valves 27. That is, they can be driven independently, i. open or closed.
  • the diaphragm valves 27 can be brought or controlled to a state that does not allow liquid flow in the respective channel, a state that allows unimpeded liquid flow in the respective channel, and / or a state that allows a reduced liquid flow in the respective channel.
  • a defined and / or simultaneous fluid intake and / or fluid delivery via the respective fluidic interfaces 5 can be controlled.
  • FIGS. 12c and 12d show an embodiment of the fluidic system with a distributor system 26 in which the channel leading away from the chamber 2 at one point branches in a star shape into four further channels.
  • a rotary valve 28 can be arranged, which can be operated manually or by means of a device from the outside. By means of the rotary valve 28 can thus be a targeted fluid flow between the leading away from the chamber 2 channel and one or more connected to the branched, ie connected to the fluidi rule interfaces 5 channels.
  • the body of the rotary valve 28 may itself have one or more recessed channels 29 which, when appropriately positioned at the point of branching capable of forming the seat 28a of the rotary valve 28, interconnect the branched and / or connected channels.
  • the option with a rotary valve 28 Depending on the design of a distribution channel 29 integrated in the rotary valve body 28b, the sequential or parallel liquid intake and / or delivery can be controlled via one or more fluidic interfaces 5, which in turn is controlled by the change in the chamber volume. It is also possible to combine one or more diaphragm valves 27 and / or rotary valves 28 in a fluidic system. That is, by means of rotary valves 28, the individual fluidic interfaces 5 can be selectively controlled and / or controlled. That is, they can be controlled independently of each other.
  • the structured component 1 has a flexible region 7 below the chamber 2, which is realized either by the application of another component in the structured component 1 or directly on the Materi aleigenschaft the structured component 1 itself or by the production of more than a material eg is implemented by multi-component injection molding.
  • FIGS. 14a and 14b A further embodiment is shown in FIGS. 14a and 14b as a top view and / or as a sectional view, wherein at a defined position above or below the chamber 2 and / or the channel system 3 a magnification function component 42 is incorporated into the structured component 1, e.g. is formed in the form of fin, in order to better track the achievement of certain positions in the channel system 3 by the liquid and also to be able to better read color reactions as indicator reactions.
  • a magnification function component 42 is incorporated into the structured component 1, e.g. is formed in the form of fin, in order to better track the achievement of certain positions in the channel system 3 by the liquid and also to be able to better read color reactions as indicator reactions.
  • FIGS. 15 a to 15 c A further embodiment is shown in FIGS. 15 a to 15 c, wherein longer channel elements than flow limiter 43 are introduced into the fluid path in the channel system 3 in order to enable a controlled liquid intake and delivery.
  • the flow restrictors are meandering forms and / or be formed as a channel taper 41 to control the flow of a fluid and / or limit the speed.
  • the chamber 2 can be connected to a plurality of channels and / or channel systems 3, each opening into at least one fluidic interface 5.
  • the fluidic system may thus have a plurality of fluidic interfaces 5 and the chamber 2 may have several outgoing channels and / or Ka nalsysteme 3.
  • Fig. 16 shows an embodiment of the fluidic system (chips) in a top view. It is the structured component 1 with a chamber 2 and the channel system 3 shown. The channel system 3 connects the input 5.1. with the chamber 2 and connects the chamber 2 to the output 5.2.
  • a Flußbe dictionary 43 is integrated, which is meandering and / or channel tapers 41 (not shown here) may contain, with the flow rate of the fluid can be controlled and / or reduced.
  • a reservoir interface 17 is ruled out with a liquid reservoir.
  • the entrance 5.1. and output 5.2 can be closed with a cap 14 which is secured by means of a pocket 44 on the chip.
  • a cap 14 is provided, which can be alternately plugged onto the input 5.1 or output 5.2, thus selectively the chip in the Fage to add fluids if input 5.1 is open, ie without cap 14, and output 5.2. is closed with the cap 14.
  • a necessary negative pressure can be built up to receive a fluid via the fluidic interface 5.1 (input).
  • the fluid should be released again.
  • the cap 14 is then placed on the input 5.1 and this closed fluid-tight. Then the fluid through the output 5.2. be delivered.
  • a circuit between two functions of the chip can be made possible.
  • a plurality of caps 14 on the chip to allow, for example, a transport of the chip or a storage of it, wherein either the interior of the chip is protected from contamination and / or leakage from inside before existing Fluids is prevented.
  • a fluidic system comprising a structured component 1 with a chamber 2 and a channel system 3, wherein at least the chamber 2 is fluid-tightly sealed with a component 4 and is fluidically connected to the outside world via the channel system 3 and a fluidic interface 5, wherein the component 4 has a flexible or movable region 6 which can be moved at least into a region of the chamber 2 or beyond a plane of the chamber 2, fluid or gas passing through the fluidic fluid by movement of the flexible or movable region 6 Interface 5 can be recorded or delivered and / or moved in the fluidic system, wherein the flexible or movable portion 6 is movable by hand or with an operating device and a pushing or moving up of the flexible or movable portion 6 is possible.
  • the invention relates to a fluidic system, comprising: a flat structured component 1 having a chamber 2 and a channel system 3, wherein at least the chamber 2 is fluid-tightly sealed with at least a component 4, the chamber 2 via the channel system 3 and at least one fluidic Interface 5 is fluidly connected to the outside world, wherein the component 4 and / or the structured component 1 has a flexible or movable portion 6, which at least partially adjacent to the chamber 2, wherein the flexible or movable portion 6 is formed, manually or with an operating device pushed into the chamber 2 or moved out of the chamber 2, so that liquids or gases are received or discharged via the at least one fluidic interface 5 and / or moved in the fluidic system.
  • a fluidic system may comprise: a structured component 1 having a chamber 2 and a channel system 3, the chamber 2 and the channel system 3 being fluid-tightly sealed with a component 4, the chamber 2 being connected via the channel system 3 and the fluidic interface 5 is fluidly connected to the outside world, wherein the structured component 1 has a flexible or movable portion 6, the side walls of the chamber 2 forms.
  • a fluidic system may comprise: a structured component 1 with a chamber 2 and a channel system 3, a component 4, which closes the chamber 2 and the channel system 3 fluid-tight ver, wherein the chamber 2 via the channel system 3 and a fluidic interface 5 with the Au DTwelt is connected, and wherein the structured component 1 is designed such that a Bo of the chamber 7 is designed to be flexible and can be pressed.
  • the flexible or movable region 6 is formed on at least one side wall of the chamber 2 within the structured component 1.
  • the chamber 2 may be connected before preferably via a further channel system 3 with a further fluidic interface 5.
  • at least one of the fluidic interfaces 5 with a cap 14 is closable ver.
  • the fluidic system may further include a venting device for the chamber (2), wherein the venting device is arranged so that venting via an additional communicating with the outside world channel 25 or a gas-permeable membrane 24 can take place.
  • the fluidic system may further include an inlet channel having a passive stop and filled either by capillary action or by a change in chamber volume caused by the flexible or movable components and receiving a defined amount of fluid.
  • the fluidic system may further include an additional reagent reservoir 16.
  • the additional reagent reservoir may be configured as a blister 16.
  • the reagent reservoir 16 can comprise: a blister seat 17, which can have pointed elements 18 which are designed to puncture the overlying liquid-tightly connected blister 16, a flap 19 which can be pressed in defined manner via guide elements 20 in the blister seat 17 , whereby a defined volume dosage is possible.
  • a channel 2 leading to the channel 3 expansions 22 have.
  • a cavity or detection chamber 21 for optical readout and / or reaction observation may be coupled to the channel system 3, which preferably has different depths.
  • the outwardly facing surface of the cavity may be transparent in order to effect a reaction of the fluid by the incident light and / or to be able to optically read the reaction taking place in the detection chamber 21 or the present ingredients.
  • the component 4 and / or the structured component 1 may be transparent at least in certain areas. This allows an observation of the movement of the fluid within the channel system 3.
  • the component 4 and or the structured component 1 may also be at least partially opaque, depending on the analyzes to be carried out in order to prevent a reaction of the fluid with the incident light.
  • the fluidic system may have a lateral flow strip 23, the filling of which is made possible by an operation of the chamber 2, wherein a venting membrane 24 and / or a venting channel 25 is coupled to the lateral flow strip 23.
  • the fluidic system may comprise at least two chambers 2, wherein the at least two chambers 2 are directly connected to each other via a channel system 3a.
  • the fluidic system may have attachments 11, 12, 13 on the flexible or movable union member 6, which are either outside the chamber 2 or extend into the chamber 2.
  • the chamber 2 may comprise pre-dispensed reagents.
  • the fluidic system may have mixing mixing elements incorporated in the chamber (2).
  • mixing of fluids within the chamber (2) may be accomplished by manually moving the fluidic system and / or by a mixing device.
  • the duct system (3) may have alignment marks, which are mounted next to, below or above the channel system (3) and allow a volume indication. With the fluidic system it is possible to carry out a multiple liquid uptake and / or release.
  • the fluidic system may comprise a rotary valve 28, via which an on acquisition and / or release of fluids is controllable.
  • the fluidic system may preferably have one or more diaphragm valves 27, which are coupled to the channel system 3 and with which the intake and / or delivery of fluids can be controlled.
  • the fluidic system may preferably have a passive stop function, which is designed as a capillary stop valve, a channel taper and / or a surface modification.
  • the reagent reservoir 16 may comprise guide elements 20, which allow a multistage volume dosing.
  • the fluidic system may have a cap as a liquid-tight closure of the fluidic interface 5.
  • the cap 14 may have a flexible portion which is adapted to be pushed or pulled out after being placed on the interface to thereby move the liquid in the channel system 3.
  • the gas-permeable membrane and / or the venting device is designed ver closable.
  • the at least two chambers 2 are arranged in one and / or more levels.
  • the movable mixing elements are designed as balls or rods.
  • the fluidic system includes structural elements in the chamber 2 and / or channel system 3 to enhance mixing.
  • the fluidic interface 5 has an outlet 10, wherein by means of a geometry of the outlet 10, the volume of a dispensed liquid drop is preset.
  • the fluidic system may have a plurality of fluidic interfaces 5, which are connected to a distributor system 26 in the structured component, wherein the plurality of fluidic interfaces 5 can be specifically controlled.
  • the channel system 3 and / or the fluidic interface 5 is designed so that an independent fluid intake into the fluidic system by means of the capillary forces of the Ka nalsystems 3 takes place at the fluidic interface 5.
  • the fluidic system may include an input 5.1 and an output 5.2 disposed on one side of the system, with a cap 14 attached to the fluidic system, preferably to the structured component 1, which may be either input 5.1 or output 5.2 is plugged, so as to allow a recording of a liquid at the input 5.1 or a dispensing of liquid at the output 5.2.
  • a cap 14 attached to the fluidic system, preferably to the structured component 1, which may be either input 5.1 or output 5.2 is plugged, so as to allow a recording of a liquid at the input 5.1 or a dispensing of liquid at the output 5.2.
  • the fluidic system may preferably have a reservoir interface 17, by means of which a liquid reservoir 16 can be connected to the structured component 1.
  • the reser voir motherboard 17 may be fluidly connected to the channel system 3 and / or with the chamber 2.
  • the channel system 3 can have valves, which makes it possible to accommodate defined liquid volumes. The valve function can be witnessed and / or enhanced by surface functionalization.
  • dry reagents are arranged or held, wherein the dry reagents are taken up by the fluids flowing through and mixed with them.
  • a reagent is present at a defined position in or on the channel system 3 and colors liquid flowing over, so that thereby a reaching of a position and thus the reaching of a certain volume or a defined residence time is indicated.
  • a magnification device is arranged so that reaching at least one specific position in the channel system 3 by liquid and / or by a color reactions can be seen.
  • the enlargement device can be designed as a lens.
  • the fluidic system may preferably have elongated channel elements as flow restrictors 43, which are introduced into the fluid path of the channel system 3, in order to enable controlled liquid absorption and delivery.
  • the reservoir interface 17 may include a flap 19 to allow for defined expression of defined volumes in the blister 16.
  • geometric elements or attachments 11, 12, 13 are provided to allow a defined movement of the flexible region 6, 7, 9.
  • the flap 19 and formed as printing elements geometric elements or on sets 11, 12 are preferably on the flexible or movable portion 6, 7, 9 together ver combined, combined and / or coupled.
  • a multi-channel distribution system 26 may be provided, which opens into a ent speaking number of fluidic interfaces 5, to allow simultaneous recording and dispensing of liquids.
  • Equal distribution of liquids in the manifold system 26 may be assisted by integrated passive valves 27.
  • the channel system 3 and / or the associated distribution system 26 may have one or more valves 27, 28, len to enable a targeted fluid delivery from individual fluidic Stel 5.
  • the fluid absorption through the fluidic interface 5 can passively be effected without movement of the flexible or movable region 6, 7, 9.
  • the one or more functional elements 45 such as filters, membranes, frits, paper or similar elements, are in or on a structured component.
  • these functional elements 45 can be mounted so that they are flooded by penetrating the liquids or gases vertically (FIG. 17 a) or horizontally (FIG. 17 c).
  • FIG. 17 comprises the FIGS. 17 a, 17 b and 17 c, in which a respective fluidic system is shown in a sectional representation.
  • the fluidic system has two fluidic interfaces 5.1 and 5.2, which are also referred to as a fluidic inlet or fluidic outlet.
  • the fluidic system has a structured component 1 with a chamber 2 and a channel system 3.
  • the channel system 3 can run on the underside and / or the top of the structured component 1, wherein the channel sections are connected to each other on the bottom and / or the top of the structured component 1 by holes or openings.
  • the structured component 1 is covered in this embodiment by two components 4, namely on the bottom and on the top.
  • the structured component 1 has, in addition to the chamber 2, a reaction cavity or a cavity 47, in which a functional element 45, in particular a membrane 45 is inserted, which is arranged so that a fluid, which from a 5.1 gear in the channel system. 3 is introduced, can penetrate through the membrane 45 therethrough. Over the membrane 45, a cavity 47 is present. After passing through the membrane 45, the fluid enters the chamber 2, which can generate a negative pressure by actuation of the flexible portion 6 to suck the fluid to suck through the membrane 45, as well as output from the output 5.2.
  • FIG. 17a shows a vertical flow through the membrane 45 or the functional element 45 in only one direction (throughflow direction 46).
  • Figure l7d shows a variant in which the functional element 45 is traversed both horizontally and ver tikal by the fluid.
  • two parallel channel strands 3 are provided.
  • the flow can be active or passive. It can be a pressure or a vacuum to be created. However, a passive exchange via concentration gradients or interactions between the regions separated by the functional element 45 is also possible.
  • the functional element 45 may be a cavity 47, which is part of the channel system 3, wherein the functional element 45 is fluidically connected to the channel system 3.
  • this invention comprises a combination of several of these functional elements on the thumb pump.
  • a further functional extension learns the thumb pump, if according to the invention Rea genzien in or on the functional elements, such as filters, membrane, frits, paper or similar ele ments are applied to react with the medium or fluid flowing through, and / or with the components or Fluid on one side or the other of the chamber.
  • a time-delayed re-suspension of incorporated reagents is particularly advantageous if Partikcl / Komponcntcn are initially retained by the functional element and they should then react with the upstream reagents.
  • reagents can be applied to the structured component 1 or the at least one component 4 (cover, bottom), these reagents being present as an arrangement or array 48 in a particularly preferred variant.
  • An array may be of the same or different reagents, e.g. DNA molecules, antibodies, apatmers, etc. can be formed as a capture molecule, this can e.g. a THEN or protein arrays.
  • reaction space The range of applied reagents is referred to as the reaction space and may well be part of the channel system 3 and / or an expansion (reaction cavity, cavity 47) or Ver depression of the channel system.
  • these reagents can also be applied to the one or more functional elements 45 introduced in accordance with the invention, such as filters, membranes, frit, paper or similar elements (FIG. 18 c).
  • FIG. 18 a shows an embodiment in which an array 48 with reagents is arranged in the reaction cavity 47.
  • This array 48 with reagents is flowed through by the fluid according to the direction of flow 46 in the channel system 3 from left to right or from the input interface 5.1 to the output interface 5.2.
  • the figure l8a has only one component 4, which covers the structured component 1 from above.
  • FIG. 18b shows a view of the reaction cavity 47 from above, wherein the array 48 is arranged in the reaction cavity 47, and the reaction cavity 47 is respectively connected to the channel system 3 and the fluid flows through it.
  • FIG. 18c shows a structured component 1 which is covered in each case with a component 4 on the upper side and on the underside of the structured component 1, since the channel system 3 is arranged on both sides of the structured component 1, or on the upper side and on the upper side Bottom is arranged.
  • FIG. 18c shows an arrangement in which a functional element 45, here in particular a membrane 45 with an array 48 of reagents, for example in the form of an arrangement of fibers, is provided, which are arranged in a reaction cavity 47, the fluid being in the flow direction 46 in the channel system 3 flows from the input interface 5.1 to the output interface 5.2 via the chamber 2 upon actuation of the flexible region 6.
  • a functional element 45 here in particular a membrane 45 with an array 48 of reagents, for example in the form of an arrangement of fibers
  • FIG. 19 A particular embodiment is shown in FIG. 19, in which first the fluid is taken up via the fluidic interface, the input 5.1, then passed through the functional element (filter / membrane / frit / paper or similar element) into the chamber 2 of the thumb pump and then passed through a further functional element 45 when pressurized and discharged via the output 5.2.
  • the functional element filter / membrane / frit / paper or similar element
  • a further functional element 45 when pressurized and discharged via the output 5.2.
  • preferably serving as the input fluidi cal interface 5.1 is closed after receiving the fluid with a cap 14.
  • FIG. 19 a shows an embodiment of a fluidic system in which two functional elements 45 in the form of a filter, a membrane, a frit or a functional paper are connected in front of and behind the chamber 2.
  • the functional elements 45 may be the same but also different, that is, the functional element 45 in front of the chamber 2 may be configured as a filter, wherein the functional element 45 is configured behind the chamber 2 or between the chamber 2 and the outlet 5.2 of the fluidic system as a filter 45, membrane 45 or frit 45 or functional paper 45 which, for example, allow fluids with a different particle size to pass.
  • the structured component 1 is covered at its top and bottom, each with a component 4 to cover the channel system 3 on the top and bottom and seal.
  • the reaction cavity 47 is connected upstream of the chamber 2, wherein a first functional component 45 is arranged therein, wherein a second reaction cavity 47 is connected downstream of the chamber 2 and is provided with a further functional element 45.
  • the input is 5.1. closed with the cap 14 to the fluid upon pressurization of the chamber 2 targeted only at the output 5.2. leave.
  • the fluid is initially taken up via the fluidic interface (input 5.1), then subsequently guided through the first functional element 45 (filter / membrane / frit / paper or similar element), and subsequently when the thumb pump is pressurized by a further functional element 45 guided and finally Lich via another fluidic interface (5.2, output) by pressurizing the Kam mer 2 delivered.
  • the fluidic interface (5.1) serving as an input is preferably closed with a cap (14) or otherwise after the fluid has been taken up.
  • two functional elements 45 are likewise arranged in FIG. 20a.
  • the second functional element 45 is not directly connected to the chamber 2 ver before the output 5.2.
  • the first upstream functional element 45 after the input 5.1 is coupled to the chamber 2, so that a flow direction in the first functional element 45 can be predetermined by a suppression or overpressure in the chamber 2 at Actuate supply of the flexible portion 6.
  • the fluid 3 is passed in the parallel channel strand past the chamber 2 directly to the second functional element 45.
  • Figure 20c is then shown to close the input 5.1 with a cap, so as to Actu conditions of the flexible portion 6 of the chamber 2 to cause a flow through the first functional element 45 through the parallel channel strand to the second functional element 45 to the fluid over to hand over the output 5.2.
  • FIG. 21 A further embodiment is shown in FIG. 21, in which first the fluid is taken up via the fluidic interface (input 5.1), then passed through the first functional element 45 (filter / membrane / frit / paper or similar element) and then into the chamber 2 of FIG Dau menpumpe arrives.
  • a liquid reservoir 16 e.g. in the form of a blister
  • a mixture of fluid and supplied liquid can be done either by the addition of the liquid itself or a movement of the thumb pump or the flexible portion 6 and can be passed through a pressurization by another functional element 45 and the diluted fluid or added with the added liquid keit Fluid is selectge via the output 5.2 by pressurizing the chamber 2 ben.
  • the input 5.1 is closed after receiving the fluid with a cap 14.
  • FIG. 2la an alternative embodiment of the fluidic system is shown, in which the first functional element 45 is connected directly to a liquid reservoir 16 and wherein Liquid addition from the liquid reservoir 16, a dilution of the fluid in the functional element 45 can take place.
  • a fluid can first be taken up in the inlet 5.1 and passed through the first functional element 45, whereby, for example, certain particles can deposit.
  • FIG. 2lb the sectional view of this embodiment is shown, is shown in Fi gur 2lc to close the input 5.1 with a cap 14 so as to then make the addition of liquid from the liquid reservoir 16, wherein after actuation of the liquid reservoir 16 and the release of the liquid by pressurization of the flexible region 6 of the chamber 2, a dilution of the fluid in the first functional element 45 and also in the second functional element 45 between chamber 2 and 5.2 output is effected.
  • FIG. 22 A further embodiment is shown in FIG. 22, in which the fluid is initially taken up via the fluidic interface (input 5.1), then guided through the first functional element 45 (filter / membrane / frit / paper or similar element) and then into the chamber 2 of FIG Continuous pump arrives.
  • the fluid is initially taken up via the fluidic interface (input 5.1), then guided through the first functional element 45 (filter / membrane / frit / paper or similar element) and then into the chamber 2 of FIG Continuous pump arrives.
  • a liquid is added to the already passed through the functional element 45 fluids.
  • the addition of the liquid from the blister 16 takes place only after the passage of the functional element 45, i.
  • a mixture of fluid and supplied liquid after processing of the fluid in the functional element 45 is achieved.
  • This mixing can be done either by the addition of the fluid itself and / or movement of the thumb pump or flexible region 6.
  • the mixed fluid can then be guided via a pressurization by a further functional element 45 and discharged via the further fluidic interface (5.2, outlet) by pressurizing the chamber 2.
  • the fluidic interface 5.1 serving as the input is closed with a cap 14 after the fluid has been taken up. That when adding the liquid from the blister 16 is the 5.1. Entrance closed.
  • FIG. 23 shows a further embodiment in which the outlet 5.2 is closed by a cap 14. That is, upon pressurization of the flexible portion 6 of the chamber 2, a fluid is received via the input 5.1 and passed through the functional element 45 and then enters the chamber 2.
  • the chamber 2 is coupled to a venting membrane 24 to vent from within the system remaining gap.
  • the input 5.1 is closed by means of a cap (Fig. 23c) and there is a liquid discharge from one of liq stechniksreservoire 16, whereby the functional element 45 is flushed in front of the chamber 2 with the liquid from the one liquid reservoir 16.
  • constituents can be removed from the functional element 45, or can be triggered by the fluid of a reaction on the functional element 45.
  • the supplied liquid then collects in the chamber 2 which can be vented through the venting membrane 24.
  • Fig. 23 shows that serving as the output fluidic interface 5.2 is initially closed ver, for example, with a cap 14 and the fluid via the fluidic interface (a gear 5.1) recorded, then by the functional element (filter / membrane / frit / Paper or similar element) is guided and enters the chamber 2 of the thumb pump. After receiving the fluid, the input 5.1 is closed, preferably by a cap 14.
  • the functional element filter / membrane / frit / Paper or similar element
  • the input 5.1 is closed, preferably by a cap 14.
  • the supplied liquid collects in the chamber 2, which is vented through a venting membrane 24.
  • a complete filling of the chamber 2 can also be ensured that before flushing the functional element 45 with a liquid which dissolves the target components, they reach the output 5.2, from which the cap 14 was previously removed.
  • the liquid discharge through the outlet 5.2 takes place in this case by the fluid flow generated by the liquid reservoir 16.
  • FIG 24 a further embodiment of the fluidic system is shown similar to Figure 23, in which the chamber 2 via a further fluidic interface 5 with the outside world is a related party, the chamber 2 can be vented through this interface 5. If this additional Liche interface 5 is closed by a cap 14, not shown, the cap can be removed at the output 5.2, which keitsreservoire 16 by further liquid supply from one of the liquid, the fluid from the functional element 45 and the components detached thereby can be flushed out. It is also possible that the cap 14 remains at the outlet 5.2 and the liquid is discharged via the further interface 5.
  • FIG. 24 A further embodiment is shown in FIG. 24, in which the fluidic interface 5.2 serving as the outlet is closed at the beginning, for example with a cap 14 and the fluid via the fluidic interface, the inlet 5.1, and subsequently through the functional element 45 (filter / membrane / Frit / paper or similar element) is guided and enters the chamber 2 of the thumb pump.
  • the inlet 5.1 Upon receipt of the fluid, the inlet 5.1 is closed, preferably by a cap 14.
  • the functional element 45 is flooded and thus a component is removed or a reaction is effected with components located on the functional element 45, e.g.
  • Anti-bodies for binding the antigens of a sample reagents that cause cell fysing, salts that alter the properties of the sample, or dyes for visualization, etc.
  • the supplied liquid collects in the chamber 2, which is ventilated via a fluidic interface 5. If this fluidic interface, e.g. closed by a cap 14 and the cap 14 is removed at the output 5.2, the liquid and components detached from the functional element 45 are flushed by liquid supply from one of the liquid reservoirs 16.
  • FIG. 25 A further embodiment is shown in FIG. 25, in which the fluidic interface 5.2 serving as output is closed at the beginning, for example with a cap 14, and the fluid is taken up via the fluidic interface, the input 5.1, and subsequently through the functional element 45 (FIG. Filter / membrane / frit / paper or similar element) is guided and enters the chamber 2 of the thumb pump.
  • the input 5.1 is closed, preferably by a cap 14.
  • 25a, 25b and 25c show a further embodiment of the fluidic system in which two functional elements 45 are present and three liquid reservoirs 16 each of which can deliver a liquid and can supply the first functional element 45 or the channel system 3 ,
  • the chamber 2 is further connected to a further interface 5, which either serves to ventilate the chamber 2, so that the chamber 2 can completely fill with liquid and there a good mixing of the fluids and the liquid from the liquid reservoirs 16 can be made.
  • the further interface 5 can also be used as an alternative output. If this alternative outlet 5 is closed, the diluted fluid can also be removed via the second functional element 45 via the outlet 5.2.
  • FIG 26a, b an alternative embodiment of the fluidic system is shown, in which the first functional element 45 in the flow direction of the fluidic interface, input 5.1, flows through and the fluid then via capillary forces, surface forces, etc. or the actuation of the flexible portion 6 in Contact with the lateral flow strip 23 passes and the lateral llow strip 23 is flooded by its intrinsic suction forces with the lluid or via the designed as a gas-permeable membrane 24 fluidic interface, a negative pressure is applied, which supports the transfer of the liquid on the lateral Llow strip 23 ,
  • the entrance 5.1 is preferably closed with a cap.
  • Ligur 27a, b an alternative embodiment of the fluidic system is shown in which the first Lunktionselement 45 is flowed through in the flow direction of the fluidic interface 5.1, the Lluid then passes through another Lunktionselement 45 and the Lluid then via capillary forces, surface forces, etc. or Pressing the flexible portion 6 comes in contact with the lateral Llow strip 23 and the Lateral Llow strip 23 is flooded by its intrinsic suction with the Lluid or via the designed as a gas-permeable membrane 24 fluidic interface, a suppression is applied, which the transfer of the liquid on supports the late llow strip.
  • the input 5.1 is preferably closed with a cap.
  • Ligur 28a-28c an alternative embodiment of the fluidic system is shown, in which the first Lunktionselement 45 is flowed through in the flow direction of the fluidic interfaces, input, 5.1, the Lluid then passes through another Lunktionselement 45 and the Lluid then via capillary forces, surface forces etc or the actuation of the flexible region 6 comes into contact with the lateral llow strip 23 and the lateral llow strip 23 is flooded by its intrinsic suction forces with the lluid or via the designed as a gas-permeable membrane 24 fluidic interface, a suppression is applied, which is the transfer the liquid continues to be supported on the lateral llow strip.
  • the entrance 5.1 is preferably closed with a cap.
  • a channel which opens at any point before or after the function elements 45 but before the lateral llow strip 23 or in the region of the lateral llow strip 23 and which is connected to one or more liquid reservoirs 16, permits lipid transfer, dilution and the delivery of reagents.
  • a waste reservoir 49 can accommodate used reagents, which is preferably connected to the channel system 3 at the end of the lateral llow strip 23.
  • Reagent Array Integrated Reagents (e.g., DNA, RNA, Protein Arrays)

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  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)

Abstract

Un système fluidique contient une chambre ayant des éléments mobiles. La chambre est reliée à un canal. Le système contient au moins un composant structuré et au moins un composant qui lui est appliqué et au moins une interface fluidique qui peut être fermée au moyen d'un bouchon ou d'une soupape. En déplaçant l'élément mobile à l'intérieur et à l'extérieur de la chambre, il est possible de déplacer des liquides ou des gaz à travers un ou plusieurs canal/canaux et de les décharger ou de les absorber à partir du système situé au-dessus. Un réservoir de réactif liquide peut être connecté à la chambre de la pompe ou au système de canaux pour obtenir des dilutions ainsi que pour alimenter les composants de réaction ou les liquides de lavage. Le système peut être utilisé pour prélever, pomper, diluer, mélanger et distribuer des liquides ou des gaz. Un élément supplémentaire (filtre, membrane, fritte ou élément similaire) ou des réactifs intégrés, qui peuvent être disposés sous la forme d'un ensemble de réactifs identiques ou différents, sont prévus pour permettre la séparation, le filtrage, le fractionnement, l'enrichissement des liquides et de leurs composants, la modification des liquides et/ou de leurs composants et la détection du contenu des liquides. Le système peut être commandé manuellement ou à l'aide de dispositifs ou d'appareils simples.
EP19726597.8A 2018-05-16 2019-05-16 Système fluidique pour recevoir, délivrer et déplacer des fluides, procédé de traitement de fluides dans un système fluidique Pending EP3793736A1 (fr)

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DE102018111822.8A DE102018111822B4 (de) 2018-05-16 2018-05-16 Fluidisches System zur Aufnahme, Abgabe und Bewegung von Flüssigkeiten, Verfahren zur Verarbeitung von Fluiden in einem fluidischen System
PCT/EP2019/062679 WO2019219844A1 (fr) 2018-05-16 2019-05-16 Système fluidique pour recevoir, délivrer et déplacer des fluides, procédé de traitement de fluides dans un système fluidique

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EP (1) EP3793736A1 (fr)
CN (1) CN112423884A (fr)
BR (1) BR112020023193A2 (fr)
CA (1) CA3100268C (fr)
DE (1) DE102018111822B4 (fr)
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CA3100268C (fr) 2023-03-07
BR112020023193A2 (pt) 2021-02-09
DE102018111822B4 (de) 2021-10-07
US20210291175A1 (en) 2021-09-23
CA3100268A1 (fr) 2019-11-21
WO2019219844A1 (fr) 2019-11-21
DE102018111822A1 (de) 2019-11-21
CN112423884A (zh) 2021-02-26
RU2765214C1 (ru) 2022-01-26

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