EP3557995A1 - Préparation d'un extrait de biomasse sèche riche en polyphenols - Google Patents
Préparation d'un extrait de biomasse sèche riche en polyphenolsInfo
- Publication number
- EP3557995A1 EP3557995A1 EP17825851.3A EP17825851A EP3557995A1 EP 3557995 A1 EP3557995 A1 EP 3557995A1 EP 17825851 A EP17825851 A EP 17825851A EP 3557995 A1 EP3557995 A1 EP 3557995A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- extract
- biomass
- treatment
- aqueous solvent
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3499—Organic compounds containing oxygen with doubly-bound oxygen
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/05—Phenols
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Definitions
- the present invention relates to a process for preparing a dry plant biomass extract, in particular vine parts, rich in polyphenols, the extract obtained and its use for antifungal applications, in particular for the prevention and treatment of infections. fungal on post-harvest fruits and vegetables, but also for applications related to its antibacterial and antioxidant properties.
- Plant biomass extracts rich in polyphenols are known to those skilled in the art. Many studies have focused on the extraction of biomass to obtain an extract rich in polyphenols, all with several drawbacks as to the economic viability of processes, such as low yields or very long extraction times, or limiting their post-extraction use, including the use of toxic organic solvents (Goupil et al 2012, Alexa et al 2012, Sanchez et al 2008, Pezet et al 2004).
- the present invention relates to a process for the preparation of a dry plant biomass extract, in particular an extract of Vitis sp., Rich in polyphenols comprising (a) a step of extracting the dry biomass by being in contact with a solvent aqueous solution and (b) a step of recovering the aqueous phase enriched in polyphenols, characterized in in the extraction step (a), the vegetable biomass / aqueous solvent mixture is treated simultaneously or sequentially by the combination
- the invention also relates to the extract obtainable by this method and its use for antifungal applications, in particular for the prevention and treatment of fungal infections on fruits and vegetables after harvest.
- the invention relates to the preparation of a polyphenol-rich extract by extraction of dry biomass with an aqueous solvent.
- the term "aqueous solvent” means water or an aliphatic alcohol / water mixture, in particular ethanol / water.
- the alcohol / water mixture in particular ethanol / water, comprises from 10 to 70% by volume of ethanol.
- the aqueous solvent is an ethanol / water mixture comprising from 15 to 40% of ethanol.
- the plant biomass according to the invention is a biomass which is itself known to contain polyphenols, in particular trans-resveratrol, trans-e-viniferine, trans-piceatannol and trans-vitisine.
- polyphenols in particular trans-resveratrol, trans-e-viniferine, trans-piceatannol and trans-vitisine.
- Those skilled in the art are familiar with this biomass from plants known to contain these polyphenols, used alone or in mixtures, generally dicotyledonous plants such as vine, tea or Japanese knotweed, more particularly the vine.
- the biomass according to the invention we will take whole plants or parts of plants, more particularly the aerial parts of plants such as fruits, flowers, leaves, stems, branches or fragments of trunks (ceps), and their mixtures.
- the biomass may also include or consist of plant roots.
- the biomass is dry, that is to say obtained by drying plants or parts of plants mentioned above. Drying can be carried out by any method known to those skilled in the art. Advantageously, the drying is carried out by letting the plants or their parts dry in the open air. According to a preferred embodiment of the invention, the plants or their separated parts are allowed to dry in the open air which ranges from 1 month to 4 years, preferably from 1 to 2 years.
- the dry biomass consists of dry parts of Vitis-like plants, in particular Vitis vinifera Vitis labrusca, Vitis riparia, Vitis rupestris, Vitis berlandieri, Vitis amurensis, Vitis coignetiae, Vitis vulpina, Vitis acerifolia, Vitis aestivalis, Vitis rotundifolia and more particularly Vitis vinifera.
- the dry biomass comprises or consists of vine shoots.
- this dried biomass consists of plant fragments, in particular shredded plants or parts of plants.
- the fragments have a particle size of less than 1 cm, more preferably less than or equal to 5 mm, more preferably ranging from 1 to 5 mm.
- This granulometry of dry matter fragments / crumbs is measured according to the usual methods well known to those skilled in the art.
- fragmentation of dry plants will be carried out according to the usual methods known to those skilled in the art. According to a particular embodiment of the invention, these fragments are obtained firstly by shredding the dry matter to obtain fragments of 1 to 20 cm which are then ground to obtain the fragments of preferably 1 to 5 mm, which correspond to at a preferred particle size for extraction.
- the mass ratio of dry vegetable biomass to aqueous solvent ranges from 1/5 to 1/30, preferably from 1/10 to 1/20.
- the process according to the invention is characterized by the combination of at least two stresses applied simultaneously or successively to the extraction mixture (biomass / solvent):
- simultaneous or sequential mixing is meant according to the invention that the treatment with microwaves and at least the stirring and / or the pressure of less than or equal to 950 mbar are implemented at the same time, or one after the other , especially according to sequences (i) + (ii), or (i) + (iii), or (i) + (ii) + (iii), or [(i), (ii)] + (iii), or [(ii), (iii)] + (i), in the order of his choice.
- sequential treatment the skilled person may choose to repeat each sequence several times, or combine different sequences according to a predetermined program, possibly combined with one or more simultaneous processing sequences.
- the three treatments are implemented simultaneously [(i), (ii), (iii)].
- Step a) may also include ultrasonic treatment (iv). This treatment can be applied sequentially or simultaneously with treatments (i), (ii) and / or (iii) defined above.
- Electromagnetic waves with a frequency of 915 MHz and 28 GHz correspond to waves of the microwave domain.
- the frequency of the electromagnetic waves ranges from 915 MHz to 2.45 GHz.
- the frequency of the electromagnetic waves will be chosen as a function of the known industrial devices for generating electromagnetic waves on an industrial scale, in particular wave generators with frequencies below 1500 MHz, more advantageously around 915 MHz.
- the person skilled in the art will also be able to choose the power of the waves most adapted to the biomass and extracts obtained.
- the power of electromagnetic waves advantageously ranges from 300 W to 100 kW, more preferably from 1 to 75 kW.
- the power of the electromagnetic waves are chosen according to the following ratio: for 1 kg of material to extract the power of electromagnetic waves is 1 to 10 kW ideally 2 to 6 kW.
- the extraction time will be at least 20 min, or even at least 30 min and preferably less than 125 min, it being understood that too short a time can be associated with too strong power conditions. may cause degradation of the extracted products, and therefore in both cases a risk of loss of polyphenols.
- the process according to the invention is suitable for use on different volumes of biomass / solvent mixture, in particular on at least 1 L, preferably at least 5 L.
- the process according to the invention is more particularly suitable for the industrial extraction of dried biomass.
- the process will be carried out on volumes of biomass / solvent mixture of at least 30 L, which may be greater than 50 L or more than 100 L.
- the method according to the invention can be used on volumes of more than 200 L.
- the biomass / solvent mixture is subjected to stirring (ii), said stirring (ii) being implemented preferentially at the same time as the electromagnetic wave treatment (i).
- stirring (ii) being implemented preferentially at the same time as the electromagnetic wave treatment (i). This mixing is important to allow the electromagnetic waves to reach the entire mixture, otherwise only the surface of the mixture would be subjected to the action of these waves.
- the person skilled in the art knows the means adapted to the implementation of a mixing according to the volumes of mixture to be treated.
- means are known for rotating the mixture to be treated, generally by means of rotating blades inside the mixing, or alternatively means for rotating the reactor containing the mixture to be treated.
- the skilled person may consider other means of mixing, such as setting the mixture in motion by bubbling air or inert gas.
- the stirring is carried out by rotating the mixture at a speed of rotation ranging from 3 to 20 revolutions per minute, more preferably by means of rotating blades inside the mixture.
- the method also comprises depressurizing (iii) the mixture, preferably during the electromagnetic wave (i) treatment and the stirring (ii).
- the method also includes ultrasound treatment, the man will know how to choose the frequency as the power of the ultrasound.
- the ultrasound frequency ranges from 25 kHz to 1 MHz.
- the power of the ultrasound ranges from 200 to 4000 W.
- the invention also relates to a process as defined above and in the examples, which comprises an additional step (c) of concentration of the plyphenols by partial or total evaporation of the aqueous solvent.
- this concentration (c) is by lyophilization of the recovered aqueous phase.
- the dried biomass is subjected, prior to the extraction step a), to leaching with a suitable solvent to eliminate the sugars.
- a suitable solvent to eliminate the sugars.
- the invention also relates to a biomass extract rich in polyphenols that can be obtained by the process according to the invention.
- These extracts advantageously have 4 molecules of interest which are polyphenols of the stilbene family. These 4 molecules are trans-piceatanol, trans-resveratrol, trans-e-viniferine and trans-vitisine.
- the extract contains at least 2 times more trans-e-viniferine (or at least 2.5 times more) at least 3 times more trans-vitisine (or at least 6 times more) than an ethanolic extract conventional.
- the invention also relates to a biomass extract rich in polyphenols that can be obtained by the process according to the invention for a biomass consisting of dry parts of plants of the genus Vitis, in particular Vitis vinifera Vitis labrusca, Vitis riparia, Vitis rupestris, Vitis berlandieri, Vitis amurensis, Vitis coignetiae, Vitis vulpina, Vitis acerifolia, Vitis aestivalis, Vitis rotundifolia and more particularly Vitis vinifera.
- the extract rich in polyphenols is an extract of vine shoots, more particularly vine shoots of Vitis vinifera.
- the extracts according to the invention have fungicidal, bactericidal and antioxidant properties, as shown by the examples for a vine extract of Vitis vinifera.
- the extracts according to the invention are active as fungicides for controlling (preventing) the development of harmful fungi in the diet and in agriculture, such as canned fruit contaminants (Byssochlamys nivea), or diseases of fruit trees, vegetables and plants, such as apple canker (Nectria galligena), fruit tree monilosis (Monilinia fructicola, Monilinia fructigena and Monillinia laxa), carrot alternaria (Alternaria daucil), the silver gall of the potato (Helminthosporium solani), the fusariosis of corn (Gibberella zeae), the fusariosis of wheat (Fusarium culmorum), the septoria of wheat (Mycosphaerella graminicola), or various rots (in particular Penicillium digitatum, Penicillium italicum) Phytophthora syringae or Rhizopus stolonifer).
- apple canker Nectria galligena
- the invention also relates to a plant fungicidal treatment method for preventing or controlling the development of pathogenic fungi on said plants, said method comprising the application to said plants of an effective amount of extract according to the invention, in particular of vine shoot extract as defined above and in the examples.
- the plants which can be treated with the extracts according to the invention are advantageously plants whose culture is intended for human consumption, such as market garden crops, potato crops, field crops such as cereal crops, in particular barley, wheat and maize, soybean or oilseed crops such as rapeseed, sunflower, flax, hemp, or cotton. Treatment can also be applied on fruit trees, especially apple, pear, apricot, peach, or on the vine.
- the application on the plants is done as a need, either as a preventive measure, when the climatic conditions are favorable for the development of the mushrooms, or because of the curative after the appearance of the diseases.
- the application will generally be made according to usual methods of spraying.
- Those skilled in the art will be able to determine when and how to treat the plants with an extract according to the invention, used alone or as a mixture with other products known for their fungicidal properties, such as copper sulphate, Bordeaux mixture, or other plant protection products from the agrochemical industry.
- the treatment is done without using pesticides from the agrochemical industry, for organic or biodynamic farming.
- the invention also relates to a method for preventing and / or treating post-harvest fruit and vegetable degradation, in particular the development of mushroom rot, said method comprising applying to said fruits and vegetables an appropriate amount of extract capable of be obtained by the method according to the invention as defined above and in the examples.
- the extracts according to the invention in particular extracts of vine shoots, can also be used for the preservation of food compositions or cosmetic compositions.
- the extract according to the invention in particular vine extract, can be used to prevent and / or treat oxidative stress in plants, by application to these plants. or on parts of plants, especially on oxidation-sensitive fruits such as bananas or tropical or exotic fruits such as mangoes, passion fruits, etc., which are sensitive to oxidative stress when stored at lower temperatures, lower than those encountered in their natural environment.
- the invention also relates to the use of the extract according to the invention, in particular the extract of vine shoots, or a composition which contains it for the prevention and / or the treatment of oxidative stress in plants.
- the extract according to the invention in particular vine shoot extract, can also be used for its antioxidant properties for the prevention and treatment of oxidative stress in humans and animals.
- the extracts according to the invention in particular extracts of vine shoots, have a broad-spectrum antimicrobial activity, with greater efficacy on skin pathogens.
- the invention therefore relates to an extract according to the invention, particularly a vine shoot extract, for its use in therapy, in particular for the treatment and prophylaxis of bacterial infections, more particularly for the treatment and prophylaxis of cutaneous bacterial infections. , especially for the treatment and prophylaxis of acne.
- the invention also relates to a composition
- a composition comprising an extract obtainable by the process according to the invention as defined above and in the examples in the examples and a suitable vehicle for its use.
- compositions intended to be applied to the plants before or after harvest, or a pharmaceutical composition.
- a pharmaceutical composition Such compositions are known and those skilled in the art will be able to determine the appropriate vehicle with respect to this use.
- the present invention relates in particular to a phytopharmaceutical composition
- a phytopharmaceutical composition comprising at least the extract as described above and at least one compatible formulation agent.
- This phytopharmaceutical composition may be in dry form, for example in the form of a powder or in the form of granules, or in liquid form, for example in the form of a suspension, a concentrated or non-concentrated dispersion, a gel, an emulsion, etc.
- the formulating agent is a natural agent or derived from synthetic chemistry, and conventionally used in phytopharmaceutical compositions.
- the formulating agent may be a dispersant, a stabilizer, a surfactant, a preservative, a wetting agent, an adhesion agent, a buffer, a pH regulator, a photoprotector, etc. They may be used alone or as a mixture .
- the extract obtainable by the process according to the invention as the compositions containing it are suitable in particular for use in the prevention and treatment of fungal infections on fruits and vegetables after harvest.
- the method according to the invention comprises the implementation of several combined techniques on the same extraction medium.
- Each technique electromagtic waves, ultrasound, depression
- FIG. 1 represents a comparison of the relative amounts of trans-piceatanol, trans-resveratrol, trans-e-viniferine and trans-vitisin contained in an extract obtained by the process according to the invention and in an extract obtained by a conventional method.
- the displayed values represent the relative concentrations (in arbitrary units) of each of the 4 molecules, calculated from the areas under the curve of the HPLC peaks.
- FIG. 2 represents the comparison of the antifungal activity of an extract obtained by the process according to the invention (round abbreviations) and of an extract obtained by a conventional process (square acronyms).
- the starting material is Vitis vinifera (baked shoots).
- the shoots are dried (in the open air or in an oven). Once dry, the shoots are first shredded into fragments of 2 to 10 cm and then finely ground to a particle size of between 1 and 5 mm.
- the vitis vinifera shoot powder thus obtained is extracted in a 30% aqueous or ethanolic solution.
- the extraction time is between 30 min and 1 h 30, preferably 45 min to 60 min.
- the techniques applied to extract are microwaves, ultrasounds vacuum and soldering simultaneously.
- the extract thus obtained is filtered under vacuum at 20 microns.
- the extract is then evaporated under vacuum and then spray-dried or lyophilized.
- the dry extract thus obtained is then stored at room temperature in the dark.
- the column used contains a stationary phase grafted with functions C18. UV detection is carried out between 200 and 800nm. The mass detection is carried out in ESI, in negative mode. Four major compounds are obtained whose retention times, absorbances and molecular weights are shown in the table below.
- Botrytis cinerea spores are deposited at the bottom of wells of 96-well transparent plates, in which nutritive agar medium was previously cast.
- the relative density of the mycelium in each well is measured via an absorbance measurement at 800 nm.
- the antifungal efficacy is then calculated from these absorbance values.
- FIG. 2 The antifungal efficacy, observed at 4 days of incubation, of an extract obtained with the process according to the invention and of an extract obtained with a conventional method is represented in FIG. 2. The results are similar to those presented in FIG. Figure 2 until at least 15 days of incubation.
- MICs Minimum Inhibitory Concentrations
- the MICs of the extract of Example 2 were determined for different species of bacteria. The tests are performed on 27 bacterial strains listed in the table below. The study is conducted in rich nutrient media, with a concentration of tested colonies calibrated at 5.10 5 -10 6 CFU / mL.
- Example 2 shows an inhibitory activity on all the tested strains except Lactobacillus plantarum and Vibrio anguillarum.
- the most sensitive strain tested is P. acnes a pathogen responsible for acne.
- bacteria of the order Bacillales, Enterococcus sp. and some Streptococcus sp. are the most sensitive to the extract according to Example 2.
- the other strains tested are less sensitive, including the beneficial bacteria Bifidobacterium sp. and Lactobacillus sp, as well as Gram-negative bacteria.
- the extract of Example 2 is a broad-spectrum antimicrobial with greater efficacy on skin pathogens.
- the antioxidant activity of the extract of Example 2 is studied by comparing the state of the skin of bananas kept in the open air with or without application of the extract of Example 2 at 7 days and 9 days. after application.
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Abstract
Description
Claims
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FR1663205A FR3061176B1 (fr) | 2016-12-22 | 2016-12-22 | Preparation d'un extrait de biomasse seche riche en polyphenols |
PCT/EP2017/084116 WO2018115296A1 (fr) | 2016-12-22 | 2017-12-21 | Préparation d'un extrait de biomasse sèche riche en polyphenols |
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EP3557995A1 true EP3557995A1 (fr) | 2019-10-30 |
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US (2) | US11517015B2 (fr) |
EP (1) | EP3557995A1 (fr) |
JP (2) | JP2020509993A (fr) |
KR (2) | KR20240087766A (fr) |
CN (1) | CN110213967A (fr) |
AU (1) | AU2017382943B2 (fr) |
BR (1) | BR112019012712A2 (fr) |
CA (1) | CA3046113A1 (fr) |
CL (1) | CL2019001748A1 (fr) |
FR (1) | FR3061176B1 (fr) |
IL (1) | IL267396B (fr) |
MA (1) | MA47080A (fr) |
WO (1) | WO2018115296A1 (fr) |
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KR102565532B1 (ko) * | 2023-01-31 | 2023-08-09 | 경북대학교 산학협력단 | 피토케미컬 추출방법 및 피토케미컬 추출액을 유효성분으로 포함하는 식물 영양제 제조방법 |
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US7314634B2 (en) | 2002-02-22 | 2008-01-01 | Steven Hernandez | Use of polyphenols to treat skin conditions |
JP2004089786A (ja) | 2002-08-29 | 2004-03-25 | Croda Japan Kk | 植物抽出物の抽出方法 |
US20070243271A1 (en) | 2003-01-13 | 2007-10-18 | Steven Hernandez | Use of polyphenols to treat skin conditions |
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BRPI0925040A2 (pt) * | 2009-04-03 | 2015-07-21 | Dianaplantsciences Inc | Produção e extração de procianidinas a partir da cultura celular de plantas |
CN101816349B (zh) | 2010-05-20 | 2012-08-15 | 北京市科威华食品工程技术有限公司 | 速溶茶膏及其加工工艺 |
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CN102757509B (zh) | 2011-04-29 | 2014-12-03 | 北京联合大学生物化学工程学院 | 一种香菇多糖的提取和纯化方法 |
CN102188463A (zh) | 2011-05-23 | 2011-09-21 | 黑龙江大学 | 榛叶提取物的制备方法 |
FR2976061B1 (fr) | 2011-05-31 | 2013-08-23 | Idco | Dispositif de traitement thermique, en particulier de materiaux divises, au moins par rayonnement micro-ondes |
FR2976062B1 (fr) | 2011-05-31 | 2013-07-19 | Idco | Procede de traitement thermique flash, par rayonnement micro-ondes et dispositif associe |
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CN103875842B (zh) * | 2014-04-16 | 2016-03-30 | 江苏安康生物科技发展有限公司 | 一种抗电磁辐射的保健茶及其制备方法 |
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CN104177463A (zh) | 2014-09-04 | 2014-12-03 | 南京标科生物科技有限公司 | 一种从刺槐花中提取刺槐苷的方法 |
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CN104256641B (zh) | 2014-10-09 | 2015-08-26 | 宁波海通食品科技有限公司 | 一种以热风脱水大麦苗粉为原料制备速溶调理麦绿素产品的方法 |
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CN104435135B (zh) | 2014-12-04 | 2017-01-25 | 黑龙江中医药大学 | 从川楝子和延胡索中提取非生物碱的方法 |
CN205759861U (zh) * | 2016-05-19 | 2016-12-07 | 江西富之源生物科技有限公司 | 一种大规模茶多酚高效分离的萃取装置 |
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US11517015B2 (en) | 2022-12-06 |
KR20190098983A (ko) | 2019-08-23 |
IL267396A (en) | 2019-08-29 |
AU2017382943A1 (en) | 2019-07-25 |
US20230120978A1 (en) | 2023-04-20 |
FR3061176A1 (fr) | 2018-06-29 |
WO2018115296A1 (fr) | 2018-06-28 |
JP2022188772A (ja) | 2022-12-21 |
FR3061176B1 (fr) | 2020-09-11 |
AU2017382943B2 (en) | 2023-01-19 |
CL2019001748A1 (es) | 2019-10-25 |
CA3046113A1 (fr) | 2018-06-28 |
KR20240087766A (ko) | 2024-06-19 |
US20200077647A1 (en) | 2020-03-12 |
JP2020509993A (ja) | 2020-04-02 |
MA47080A (fr) | 2019-10-30 |
BR112019012712A2 (pt) | 2019-11-26 |
CN110213967A (zh) | 2019-09-06 |
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