EP3476489B1 - Insert de centrifugeuse - Google Patents

Insert de centrifugeuse Download PDF

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Publication number
EP3476489B1
EP3476489B1 EP18201796.2A EP18201796A EP3476489B1 EP 3476489 B1 EP3476489 B1 EP 3476489B1 EP 18201796 A EP18201796 A EP 18201796A EP 3476489 B1 EP3476489 B1 EP 3476489B1
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EP
European Patent Office
Prior art keywords
centrifuge
insert
centrifuge insert
sample
elements
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Application number
EP18201796.2A
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German (de)
English (en)
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EP3476489A1 (fr
Inventor
Robert GÖLLNITZ
Sebastian RYK
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Eppendorf SE
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Eppendorf SE
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Publication of EP3476489A1 publication Critical patent/EP3476489A1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B04B7/12—Inserts, e.g. armouring plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B2005/0435—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles with adapters for centrifuge tubes or bags

Definitions

  • the present invention relates to a centrifuge insert for holding one or more elements from the group of sample containers and sample carriers in a laboratory centrifuge, which is specified in the preamble of claim 1.
  • Centrifuge rotors are used in centrifuges, especially laboratory centrifuges, to separate the components of samples centrifuged therein using inertia. In order to achieve high segregation rates, ever higher rotation speeds are used.
  • Laboratory centrifuges are centrifuges whose rotors work at preferably at least 3,000, preferably at least 10,000, in particular at least 15,000 revolutions per minute and are usually placed on tables. In order to be able to place them on a work table, they in particular have a form factor of less than 1 m ⁇ 1 m ⁇ 1 m, so their installation space is limited.
  • the device depth is preferably limited to a maximum of 70 cm.
  • samples be centrifuged at certain temperatures.
  • samples that contain proteins and similar organic substances must not be overheated, so that the upper limit for temperature control of such samples is standard in the range of +40°C.
  • certain samples are cooled by default in the +4°C range (the water anomaly starts at 3.98°C).
  • Active and passive systems can be used for temperature control.
  • Active cooling systems have a refrigerant circuit that controls the temperature of the centrifuge bowl, thereby indirectly cooling the centrifuge rotor and the sample containers contained therein.
  • Passive systems are based on exhaust air-supported cooling or ventilation. This air is led directly past the centrifuge rotor, which results in temperature control. The air is sucked into the centrifuge bowl through openings, with the suction taking place automatically through the rotation of the centrifuge rotor.
  • the samples to be centrifuged are stored in sample containers or sample carriers and these sample containers are rotationally driven by the centrifuge rotor.
  • the centrifuge rotors are usually set in rotation by means of a vertical drive shaft that is driven by an electric motor. There are different centrifuge rotors that are used depending on the application.
  • centrifuge rotors usually have a lower part and a lid, with an interior space forming between the lower part and the lid in the closed state of the lid, in which the sample containers can be arranged in order to centrifuge the samples in a suitable centrifuge. If the sample containers or sample carriers are arranged at a fixed angle in the centrifuge rotor, then it is a so-called fixed-angle rotor. If, on the other hand, the sample containers or sample carriers are arranged to be pivotable in a radial direction with respect to the axis of rotation of the centrifuge rotor, then it is a swing-out rotor.
  • the centrifuge inserts are used to arrange and centrifuge the sample containers or sample carriers in the rotors of the centrifuges.
  • swing-bucket rotors which have a pivotable centrifuge cup into which such a centrifuge insert can be adjusted.
  • sample containers such as sample bottles, sample cups, sample tubes, tubes, reaction vessels, centrifuge vessels, bottles, microreaction vessels or cell culture bottles, which can also be available in different sizes. These sample containers can also have different bottom designs, for example flat, conical or rounded.
  • sample carriers such as microplates, microtiter plates (MTP), PCR plates and deep well plates (DWP). In general, it can be stated that sample containers have a single receptacle for samples and sample carriers have a plurality of receptacles for samples.
  • centrifuge container which can be suspended in a swing-out rotor, the centrifuge container having a special geometric design of its inner contour. More specifically, there are wall sections that define a receiving area for a substantially cuboid sample carrier, and there are wall sections that define a cylindrical receiving area for a centric sample bottle.
  • the DE 20 202013 006 223 U1 shows a centrifuge container in which sample holders are set to hold sample tubes.
  • the sample holder insert consists of a lower part and an upper part, which can optionally be connected to one another can be combined, whereby either only the lower part or the lower part and upper part can be combined with each other.
  • the upper part is always placed on the lower part and, if necessary, locked so that short or long sample tubes can be held.
  • the US 2016/250608 A1 shows a sample shaker in which there is a shaking device with a connecting piece onto which a carrier can be attached.
  • the carrier has a central area in which there are receptacles for the vertical arrangements of sample containers. On the side of the central area there are four bowl-like receptacles for horizontally holding the corresponding sample containers.
  • the object of the present invention is to eliminate these disadvantages.
  • a centrifuge insert which is suitable not only for one type of sample container or sample carrier, but for at least two geometrically differently designed elements from the group of sample containers and sample carriers.
  • One type should have a cross-section that is at least partially circular or oval and the other type should have a cross-section that is at least partially polygonal, in particular rectangular. This means that geometrically different elements can be centrifuged using the centrifuge insert with the centrifuge rotor.
  • centrifuge insert could also be adapted to accommodate both different elements at the same time.
  • This solution according to the invention ensures that the same centrifuge insert can still be used with geometrically different elements. This leads to space savings, especially in the laboratory. This also reduces the acquisition costs.
  • the centrifuge insert according to the invention for receiving one or more elements from the group of sample containers and sample carriers in a centrifuge rotor, preferably designed as a swing-out rotor, of a centrifuge, in particular a laboratory centrifuge, is therefore characterized in that the centrifuge insert is adapted, at least a first element, which is at least partially has a circular or oval cross-section, and to optionally accommodate at least one second element, which has a polygonal cross-section at least in some areas.
  • the polygonal cross section is preferably a rectangular cross section.
  • the first element is a sample container, preferably a sample bottle, a sample tube, a tube, a reaction vessel, a centrifuge vessel, a bottle, a microreaction vessel or a cell culture bottle and in particular has a conical, flat or rounded bottom design .
  • Standard sample containers can then be centrifuged using the centrifuge insert.
  • the second element is a sample carrier, preferably a plate-shaped element, in particular in the form of a microplate, a microtiter plate, a PCR plate or a deepwell plate, with several receptacles for samples. Then you can use usual sample carriers Centrifuge with numerous holders for a variety of samples using the centrifuge insert.
  • the centrifuge insert is adapted to accommodate the elements in a form-fitting manner. This means that the elements are held very securely even during centrifugation.
  • the bottom design of receptacles of the centrifuge insert is adapted to the bottom design of sample containers and/or sample carriers to be accommodated and preferably has a flat, conical or rounded contour. This ensures that the sample containers or sample carriers are held particularly securely in the centrifuge insert.
  • the centrifuge insert is designed to be modular with a base body and at least one supplementary part, the supplementary part being designed to be adjustable into the base body, in particular to be plugged in and/or set up on the base body, in particular to be plugged on, with a plug-in connection in particular between the base body and the supplementary part.
  • the plug connection provides a force and/or positive connection, preferably has clamping properties. This means that the additional part is held particularly securely in the base body.
  • the supplementary part has one or more receptacles for first elements.
  • the supplementary part has an external geometry which, together with a corresponding internal geometry of the base body, forms one or more receptacles for first elements. This means that the centrifuge insert can be designed in a particularly variable manner and can still accommodate a maximum number of sample containers.
  • the centrifuge insert is designed for lateral fixation of the first and/or the second elements.
  • a lateral fixation can be achieved, for example, by depressions or grooves that are present at least in some areas and/or by webs or projections that exist at least in some areas and which correspond to corresponding elements, such as edges, of the first and second elements.
  • This enables a very simple and yet effective recording, and by providing different grids of such depressions or webs and projections, differently sized first and second elements can also be accommodated.
  • Such a second element can be designed as a sample carrier, for example as a microtiter plate. Such a second element can be at least partially enclosed laterally by the webs or projections and/or the depressions or grooves.
  • the centrifuge insert is designed for vertical fixation of the first and/or second elements.
  • a vertical fixation could be achieved, for example, by a snap and/or clip connection.
  • the webs or projections could lock with a peripheral edge of the first or second element.
  • the centrifuge insert is adapted, the first elements being inserted into corresponding receptacles of the centrifuge insert. Then the first elements, especially in the form of sample containers, are held very securely.
  • the centrifuge insert is adapted to carry the second elements, the second elements preferably being fixed laterally in a form-fitting manner on a base surface. Then the second elements, especially in the form of sample carriers, are held very securely.
  • the centrifuge insert has at least two guides arranged opposite each other, which are adapted to laterally support at least one supplementary part and/or at least one second element, preferably at least two supplementary parts or second elements stacked on top of each other. This ensures particularly secure recording.
  • the guides are designed as handles for gripping the centrifuge insert.
  • the centrifuge insert can then be transported particularly easily and manipulated in a centrifuge.
  • the handles can also not fulfill a guiding function, i.e. they can only be intended for the handle function.
  • the guides have locking connection elements which are adapted to provide a locking connection between the guides and the supplementary part and/or the second element. This makes the recording even better secured.
  • a preferred embodiment of the centrifuge insert 10 according to the invention is shown in various views.
  • FIG. 1 shows Fig. 1 the base body 12 of the centrifuge insert 10, which Fig. 2a and 2 B show two supplementary parts 14, 16 for this base body 12 and the Fig. 3 to 5 show various possible combinations of base body 12 and additional parts 14, 16 as well as the resulting applications.
  • the base body 12 is essentially octagonal in cross section and has a central recess 18, which corresponds to six cylindrical segment-shaped recesses 20 which are equally spaced with respect to the circumference. Between two adjacent recesses 20 there is a vertically extending groove 22, which springs back outwards in relation to the central recess 18.
  • a central recess 18 which corresponds to six cylindrical segment-shaped recesses 20 which are equally spaced with respect to the circumference.
  • a vertically extending groove 22 which springs back outwards in relation to the central recess 18.
  • handles 36 extending vertically upwards from the base surface 32 are provided, each of which comprises an approximately semi-cylindrical space 38 and has a top surface 40 at the top. This provides a handle 36 for gripping the centrifuge insert 10, which can be gripped particularly securely through the spaces 38.
  • An edge 42 extends below the base surface 32, relative to which the outer walls 44 of the central recess 18 and the recesses 20 extend radially back.
  • a first supplementary part 14 for the base body 12 is shown, which is essentially designed as a hollow cylinder with a wall 46 on which outwardly projecting ribs 48 extend at equal radial distances.
  • the wall 46 has an outer circumference which is matched to the inner circumference of the central recess 18.
  • the ribs 48 run downwards, becoming more pointed and becoming flatter on the other hand, and correspond to the grooves 22, which also become narrower and flatter away from the base surface 32.
  • a large axial receptacle 59 is formed here, which can positively accommodate a circular cross section of a first element, for example a sample container.
  • a second supplementary part 16 is shown for the base body 12, which is essentially star-shaped.
  • the base body 12 In the middle there is an axial receptacle 60 and on the edge there are six cylindrical segment-shaped recesses 62 arranged at equal distances, with webs 64 extending between the recesses 62, on which ribs 66 in turn extend accordingly Fig. 2a are arranged.
  • These ribs 66 can in turn fit snugly into the grooves 22 of the base body 12 in order to achieve a clamping effect when the supplementary part 16 is inserted into the base body 12 and thus hold the supplementary part 16 securely in the base body 12.
  • the recesses 62 taper conically downwards 68, this shape corresponding to conical tapers 70 of the recesses 20.
  • receptacles 72 are formed (cf. Fig. 4 ), which taper conically at the bottom and are therefore suitable for sample containers with a conically tapered bottom area.
  • the axial receptacle 60 also has exactly the same geometry, so that there are seven identical receptacles 60, 72 here.
  • the centrifuge insert 10a is designed as a combination of base body 12 and supplementary part 14 and is shown in the swing-out cup 110 of a swing-out rotor 108 of a laboratory centrifuge 100, which is only partially shown.
  • This allows a single sample container 74, which is designed, for example, as a wide-neck bottle with a volume of 250 ml, to be centrifuged. Since the axial receptacle 59 has a width adapted to the circular cross section of the sample container 74, the sample container 74 is accommodated in the centrifuge insert 10a in a form-fitting and therefore secure manner.
  • the centrifuge insert 10b is designed as a combination of base body 12 and supplementary part 16 and is shown in the swing-out cup 110 of a swing-out rotor 108 of a laboratory centrifuge 100, which is only partially shown.
  • This allows individual sample containers 76, which are designed as sample tubes with a volume of 50 ml, to be centrifuged. Since the receptacles 60, 72 have a width adapted to the circular cross section of the sample containers 76, the sample containers 76 are also accommodated in a form-fitting and therefore secure manner in the centrifuge insert 10b.
  • the centrifuge insert 10c is designed as a combination of base body 12 and supplementary part 14 and is shown in the swing-out cup 110 of a swing-out rotor 108 of a laboratory centrifuge 100, which is only partially shown.
  • This allows, for example, individual sample carriers 78, which are designed as microtiter plates, to be centrifuged. Since the one from the webs 28, 30, 34, 34', 34" is adapted to the essentially rectangular base area 80 of the microtiter plate 78 and the edge 82 of the base area 80 can dip into the grooves 35, 35', 35" and the depressions 29, 31, the microtiter plate 78 is positively secured laterally to the centrifuge insert 10c and is therefore securely received (cf. also Fig. 9b , which shows a similar interaction). In this form of use, the supplementary part 16 could also be inserted or no supplementary part 14, 16 is inserted - the sample carrier 78 is always held securely laterally.
  • the supplementary part 16 can also be inserted into the base body 12.
  • no additional part 14, 16 needs to be inserted into the base body 12 at all, since the sample carrier 78 is supported on the base surface 32, so that the base body 12 alone can form the centrifuge insert 10.
  • Fig. 6 the centrifuge insert 10a according to the invention is shown in cooperation with a laboratory centrifuge 100 in a perspective view.
  • the laboratory centrifuge 100 has a housing 102 with a lid 104, which can close the sample space 106, in which a motor-driven (not shown) centrifuge rotor 108 is located.
  • the centrifuge rotor 108 is designed as a swing-out rotor and has centrifuge cups 110, which can pivot away from the axis of rotation D, i.e. outwards, during centrifugation.
  • the centrifuge inserts 10a according to the invention are accommodated in these centrifuge cups 110.
  • a second embodiment of a centrifuge insert 200 not according to the invention is shown in various views.
  • this centrifuge insert 200 differs from the centrifuge egg set 10 in that here the base body 202 with the handles 204 is designed without a central recess for receiving additional parts.
  • nine identical receptacles 206 are arranged directly in the base body 202, which in principle correspond to the receptacles 60, 72 of the centrifuge insert 10a and are designed with the same inner diameter.
  • vertically rising webs 210, 212, 214, 216 are provided on a base surface 208, which enclose a rectangular surface. At the same time there are recesses 211, 213 and grooves 215, 217, 218, 219.
  • the handles 204 are not arranged symmetrically with respect to the webs 210, 212, but are slightly offset so that they can be arranged between two receptacles 206. However, this does not affect the grip function of these handles 204, since they are nevertheless arranged symmetrically with respect to the center of mass, which extends in the middle of the central receptacle 206.
  • Fig. 8 the centrifuge insert 200 is shown in a first application of holding nine sample containers 220, which are designed as sample tubes with a capacity of 50 ml and can therefore be centrifuged in the laboratory centrifuge 100. Since the receptacles 206 have a width adapted to the circular cross section of the sample containers 220, the sample containers 220 are accommodated in the centrifuge insert 200 in a form-fitting and therefore secure manner.
  • the centrifuge insert 200 is shown in two views in a second application of receiving a sample carrier 222, which is designed as a microtiter plate. Since in turn the rectangular area encompassed by the webs 210, 212, 214, 216 is adapted to the essentially rectangular base area 224 of the microtiter plate 222 and the edge 226 dips into the depressions 211, 213 and grooves 215, 217, 218, 219 , the microtiter plate 222 is positively secured laterally to the centrifuge insert 200 and is therefore securely received, as shown in particular Fig. 9b clearly shows. In addition, one could There is a clip connection or latching between the webs 210, 212, 214, 216 and the edge 226 in order to also fix the sample carrier 222 vertically.
  • centrifuge insert 300 is shown in various views.
  • Fig. 10 shows the base body 302 of the centrifuge insert 300, which differs from the base body 202 of the centrifuge insert 200 in that a larger number of receptacles 304 are provided in the base surface 306.
  • These receptacles 304 are adapted to accommodate sample containers 308, which are in the form of reaction vessels with a volume of 5 ml, that is, since the receptacles 304 have a width adapted to the circular cross section of the sample containers 308, the sample containers 308 are form-fitting in the centrifuge insert 300 and therefore safely recorded (cf. in Fig. 11 corresponding first use case shown).
  • the base surface 306 does not protrude so far compared to the bottom surface 310 of the base body 302. Therefore, with respect to a specific centrifuge cup 110 (cf. Fig. 6 ) the handles 312 can be made longer.
  • this base body 302 also encompasses a rectangular surface in the form of webs 314, 316, 318, 318', 318", 318" as well as depressions 315, 317 and grooves 319, 319', 319".
  • Fig. 10 also shows a supplementary part 320, which has a base surface 322 and walls 324 extending downwards from it, which provide numerous receptacles 326, the bottom surfaces of which are missing, so that the receptacles are designed as hollow cylinders with a constant cross-section over their entire length.
  • These receptacles 326 are adapted to accommodate sample containers 328, which are in the form of sample bottles with a volume of 15 ml.
  • Fig. 12 It can also be seen that the handles 312 engage with their outer contour in the indentations 330 of the supplementary part 320 in a form-fitting manner, so that the supplementary part is fixed laterally between the handles 312 and is therefore securely arranged on the base body 302 even in centrifugation mode.
  • this design forms a threading and positioning aid for the supplementary part 320, so that the receptacles 326 of the supplementary part 320 can be positioned exactly over the receptacles 304 of the base body 302.
  • the handles 312 here also form guides for the supplementary part 320.
  • the supplementary part 320 has annular projections 336 on the receptacles 326 'at some points on its base surface 334, which engage in a positive and non-positive manner in corresponding recesses 304' of the base body 302.
  • these receptacles 304 'of the base body 302 have an additional groove 337 into which the annular projections 336 can be inserted. This results in a further lateral fixation of the supplementary part 320 relative to the base body 302.
  • the inner diameters of the receptacles 326, 326' of the supplementary part 320 and the receptacles 304, 304' of the base body 302 are identical, so that there is a continuously continuous receptacle 304, 304', 326, 326' results.
  • annular projections 336 and groove 337 Due to this non-positive and positive connection of annular projections 336 and groove 337, an additional positive or positive connection with the handles 312 could also be dispensed with.
  • the sample bottles 328 are relatively long. Due to the continuous design of the receptacles 304, 304 ', 326, 326' and the adaptation of their width to the circular cross section of the sample containers 328, the sample containers 328 are form-fitting in the centrifuge insert 300a, which is formed from the base body 302 and the supplementary part 320 and therefore safely recorded, whereby the sample containers 328 extend into the base body 302 and are held laterally by both the base body 302 and the supplementary part 320, as Fig. 12 shows.
  • a sample carrier 338 which is designed as a microtiter plate, is arranged on the base body 302. Since in turn the rectangular area encompassed by the webs 314, 316, 318, 318 ', 318", 318' is adapted to the essentially rectangular base area 340 of the microtiter plate 338 and the edge 342 in the depressions 315, 317 and grooves 319 , 319 ', 319", the microtiter plate 338 is in turn secured laterally to the centrifuge insert 300 in a form-fitting manner and is therefore securely received.
  • receptacles 400, 410, 420 of centrifuge inserts are shown purely schematically in cross-section in interaction with each recorded sample containers 402, 412, 422. It can be seen that the bottom designs of receptacles 400, 410, 420 and sample containers 402, 412, 422 are adapted to one another in such a way that there is a positive connection (the distance between the side walls is only drawn for better visibility), whereby the sample containers 402 , 412, 422 can be held particularly securely in the holders. Show it Fig. 14 a a flat, Fig. 14b a rounded and Fig. 14c a conical bottom design of the respective receptacle 400, 410, 420 and sample containers 402, 412, 422.
  • centrifuge insert according to the invention can easily be applied to other sample containers and sample carriers, in particular those with a different geometry, including the floor design, and / or volume of the recording can be adjusted.
  • the present invention provides a centrifuge insert 10, 10a, 10b, 10c, which can be used for different sample containers 74, 76 or sample carriers 78.
  • This leads to space savings, especially in the laboratory.
  • this reduces the acquisition costs and by reducing the manual effort, processes are accelerated and the laboratory throughput is increased.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)

Claims (10)

  1. Insert de centrifugeuse (10) destiné à recevoir un ou plusieurs éléments du groupe des récipients d'échantillon (74, 76) et des porte-échantillons (78) dans un rotor de centrifugeuse (108), de préférence réalisé sous la forme d'un rotor libre, d'une centrifugeuse (100), en particulier d'une centrifugeuse de laboratoire, l'insert de centrifugeuse (10) étant adapté pour recevoir au choix au moins un premier élément (74, 76), qui présente au moins par endroits une section transversale circulaire ou ovale, et au moins un second élément (78), qui présente au moins par endroits une section transversale polygonale, caractérisé en ce que l'insert de centrifugeuse (10) est réalisé de manière modulaire avec un corps de base (12) et au moins une pièce complémentaire (16), la pièce complémentaire (16) possédant une géométrie extérieure qui forme, conjointement avec une géométrie intérieure correspondante du corps de base (12), un ou plusieurs seconds logements (72) pour des premiers éléments (76).
  2. Insert de centrifugeuse (10) selon la revendication 1, caractérisé en ce que le premier élément est un récipient d'échantillon, de préférence un flacon pour échantillon (74), un bécher pour échantillon, un tube à essai (76), un tube souple, un récipient de réaction, un récipient de centrifugation, un flacon, un récipient de microréaction ou un flacon de culture cellulaire et présente en particulier une partie de fond réalisée conique et/ou en ce que le second élément est un porte-échantillons (78), de préférence un élément en forme de plaque, en particulier sous la forme d'une microplaque, d'une plaque microtitre, d'une plaque PCR ou d'une plaque Deepwell, avec plusieurs logements pour des échantillons.
  3. Insert de centrifugeuse (10) selon la revendication 1 ou 2, caractérisé en ce que l'insert de centrifugeuse (10) est adapté pour recevoir les éléments (74, 76, 78) par complémentarité de forme et/ou en ce que la configuration du fond de logements de l'insert de centrifugeuse est adaptée à la configuration du fond des récipients d'échantillon (74, 76) et/ou porte-échantillons (78) à recevoir, et présente de préférence un contour plat, conique ou arrondi.
  4. Insert de centrifugeuse (10) selon l'une des revendications précédentes, caractérisé en ce que la pièce complémentaire (16) est réalisée de manière à pouvoir être réglée, de préférence enfichée, dans le corps de base (12) et/ou de manière à pouvoir être posée, de préférence emboîtée, sur le corps de base, un assemblage par emboîtement, de préférence sous la forme d'un assemblage à rainure et languette, pouvant en particulier être formé entre le corps de base (12) et la pièce complémentaire (16).
  5. Insert de centrifugeuse (10) selon l'une des revendications précédentes, caractérisé en ce que la pièce complémentaire présente un ou plusieurs premiers logements pour des premiers éléments.
  6. Insert de centrifugeuse (10) selon l'une des revendications précédentes, caractérisé en ce que l'insert de centrifugeuse (10) est conçu pour la fixation latérale des premiers (74, 76) et/ou des seconds éléments (78).
  7. Insert de centrifugeuse (10) selon l'une des revendications précédentes, caractérisé en ce que l'insert de centrifugeuse (10) présente un ou plusieurs rebords (28, 30, 34, 34', 34") ou saillies, qui sont adaptés pour entourer latéralement, au moins en partie, au moins un des premiers et seconds éléments (78) et/ou en ce que l'insert de centrifugeuse présente un ou plusieurs creux (29, 31) ou rainures (35, 35', 35"), qui sont adaptés pour entourer latéralement, au moins en partie, au moins un des premiers et seconds éléments (78), l'insert de centrifugeuse (10) étant en particulier adapté pour recevoir les premiers éléments (74, 76) par enfichage dans des logements (59, 72) correspondants de l'insert de centrifugeuse (10) et/ou pour porter les seconds éléments (78), les seconds éléments (78) étant bloqués latéralement par complémentarité de forme de préférence sur une surface de base (32).
  8. Insert de centrifugeuse (10) selon l'une des revendications précédentes, caractérisé en ce que l'insert de centrifugeuse (10) présente au moins deux guidages (312) disposés de manière opposée, lesquels sont adaptés pour soutenir latéralement au moins une pièce complémentaire (320) et/ou au moins un second élément, de préférence au moins deux pièces complémentaires ou seconds éléments empilés les uns sur les autres.
  9. Insert de centrifugeuse (10) selon la revendication 8, caractérisé en ce que les guidages présentent des éléments d'assemblage par encliquetage, qui sont adaptés pour fournir un assemblage par encliquetage entre les guidages et la pièce complémentaire et/ou le second élément.
  10. Insert de centrifugeuse (10) selon l'une des revendications précédentes, caractérisé en ce que l'insert de centrifugeuse présente des poignées (36) pour saisir l'insert de centrifugeuse (10), lesquelles sont réalisées en particulier sous la forme des guidages selon l'une des revendications 8 ou 9.
EP18201796.2A 2017-10-27 2018-10-22 Insert de centrifugeuse Active EP3476489B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102017125306.8A DE102017125306A1 (de) 2017-10-27 2017-10-27 Zentrifugeneinsatz

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EP3476489A1 EP3476489A1 (fr) 2019-05-01
EP3476489B1 true EP3476489B1 (fr) 2023-09-20

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US (1) US11666927B2 (fr)
EP (1) EP3476489B1 (fr)
JP (1) JP6843102B2 (fr)
CN (1) CN109718951B (fr)
DE (1) DE102017125306A1 (fr)

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* Cited by examiner, † Cited by third party
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US10625273B2 (en) * 2012-09-03 2020-04-21 Eppendorf Ag Centrifuge insert and carrier for centrifuge insert with snap locking connection
CN112517103B (zh) * 2020-12-24 2024-07-19 苏州市立医院 一种可控式离心管架

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US20160250608A1 (en) * 2015-02-27 2016-09-01 Heathrow Scientific Llc Head for a mixing apparatus

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DD149615A1 (de) * 1980-03-26 1981-07-22 Roland Lippold Gefaesstraeger fuer laborzentrifugen zur aufnahme von probengefaessen
JPS59171759A (ja) 1983-03-16 1984-09-28 Hitachi Ltd 電動式パワステアリング装置
JPS59171759U (ja) * 1983-03-25 1984-11-16 株式会社久保田製作所 遠心分離機用バケツト
US4531652A (en) 1984-06-25 1985-07-30 Kabushiki Kaisha Kubota Seisakusho Bucket for use in centrifugal separators
DE3425922C2 (de) * 1984-07-13 1986-05-28 Kabushiki Kaisha Kubota Seisakusho, Tokio/Tokyo Kübel zum Aufhängen an einem Schwenkrotor einer Zentrifuge
US5549540A (en) * 1994-10-13 1996-08-27 Baxter International Inc. Centrifuge insert for support of fluid containing bags
DE102012013642B4 (de) * 2012-07-09 2020-12-24 Thermo Electron Led Gmbh Zentrifugengefäßeinheit
US10625273B2 (en) * 2012-09-03 2020-04-21 Eppendorf Ag Centrifuge insert and carrier for centrifuge insert with snap locking connection
CN202778745U (zh) * 2012-09-28 2013-03-13 黑龙江八一农垦大学 套装组合式离心机适配器
US9180461B2 (en) * 2012-10-22 2015-11-10 Qiagen Gaithersburg, Inc. Condensation-reducing incubation cover
DE202013006223U1 (de) * 2013-07-10 2014-10-13 Eppendorf Ag Probenhaltereinsatz zum Halten von mehreren Probenröhrchen in einem Laborbehälter und Laborbehälter mit diesem Probenhaltereinsatz
WO2016052265A1 (fr) * 2014-09-30 2016-04-07 日立工機株式会社 Centrifugeuse et rotor oscillant pour centrifugeuse
US20170368560A1 (en) * 2016-06-27 2017-12-28 Flotek Chemistry, Llc Centrifuge container adapter and related articles, systems, and methods
JP2018201439A (ja) 2017-06-07 2018-12-27 則雄 安井 焙煎器

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US20160250608A1 (en) * 2015-02-27 2016-09-01 Heathrow Scientific Llc Head for a mixing apparatus

Also Published As

Publication number Publication date
CN109718951A (zh) 2019-05-07
CN109718951B (zh) 2022-06-03
DE102017125306A1 (de) 2019-05-02
US20190151862A1 (en) 2019-05-23
JP2019081169A (ja) 2019-05-30
JP6843102B2 (ja) 2021-03-17
EP3476489A1 (fr) 2019-05-01
US11666927B2 (en) 2023-06-06

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