EP3476489B1 - Centrifuge insert - Google Patents
Centrifuge insert Download PDFInfo
- Publication number
- EP3476489B1 EP3476489B1 EP18201796.2A EP18201796A EP3476489B1 EP 3476489 B1 EP3476489 B1 EP 3476489B1 EP 18201796 A EP18201796 A EP 18201796A EP 3476489 B1 EP3476489 B1 EP 3476489B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- centrifuge
- insert
- centrifuge insert
- sample
- elements
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000005119 centrifugation Methods 0.000 claims description 5
- 238000004113 cell culture Methods 0.000 claims description 3
- 230000000295 complement effect Effects 0.000 claims 5
- 239000000969 carrier Substances 0.000 description 18
- 238000011161 development Methods 0.000 description 14
- 230000018109 developmental process Effects 0.000 description 14
- 238000001816 cooling Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000007373 indentation Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/08—Rotary bowls
- B04B7/12—Inserts, e.g. armouring plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
- B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
- B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B2005/0435—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles with adapters for centrifuge tubes or bags
Definitions
- the present invention relates to a centrifuge insert for holding one or more elements from the group of sample containers and sample carriers in a laboratory centrifuge, which is specified in the preamble of claim 1.
- Centrifuge rotors are used in centrifuges, especially laboratory centrifuges, to separate the components of samples centrifuged therein using inertia. In order to achieve high segregation rates, ever higher rotation speeds are used.
- Laboratory centrifuges are centrifuges whose rotors work at preferably at least 3,000, preferably at least 10,000, in particular at least 15,000 revolutions per minute and are usually placed on tables. In order to be able to place them on a work table, they in particular have a form factor of less than 1 m ⁇ 1 m ⁇ 1 m, so their installation space is limited.
- the device depth is preferably limited to a maximum of 70 cm.
- samples be centrifuged at certain temperatures.
- samples that contain proteins and similar organic substances must not be overheated, so that the upper limit for temperature control of such samples is standard in the range of +40°C.
- certain samples are cooled by default in the +4°C range (the water anomaly starts at 3.98°C).
- Active and passive systems can be used for temperature control.
- Active cooling systems have a refrigerant circuit that controls the temperature of the centrifuge bowl, thereby indirectly cooling the centrifuge rotor and the sample containers contained therein.
- Passive systems are based on exhaust air-supported cooling or ventilation. This air is led directly past the centrifuge rotor, which results in temperature control. The air is sucked into the centrifuge bowl through openings, with the suction taking place automatically through the rotation of the centrifuge rotor.
- the samples to be centrifuged are stored in sample containers or sample carriers and these sample containers are rotationally driven by the centrifuge rotor.
- the centrifuge rotors are usually set in rotation by means of a vertical drive shaft that is driven by an electric motor. There are different centrifuge rotors that are used depending on the application.
- centrifuge rotors usually have a lower part and a lid, with an interior space forming between the lower part and the lid in the closed state of the lid, in which the sample containers can be arranged in order to centrifuge the samples in a suitable centrifuge. If the sample containers or sample carriers are arranged at a fixed angle in the centrifuge rotor, then it is a so-called fixed-angle rotor. If, on the other hand, the sample containers or sample carriers are arranged to be pivotable in a radial direction with respect to the axis of rotation of the centrifuge rotor, then it is a swing-out rotor.
- the centrifuge inserts are used to arrange and centrifuge the sample containers or sample carriers in the rotors of the centrifuges.
- swing-bucket rotors which have a pivotable centrifuge cup into which such a centrifuge insert can be adjusted.
- sample containers such as sample bottles, sample cups, sample tubes, tubes, reaction vessels, centrifuge vessels, bottles, microreaction vessels or cell culture bottles, which can also be available in different sizes. These sample containers can also have different bottom designs, for example flat, conical or rounded.
- sample carriers such as microplates, microtiter plates (MTP), PCR plates and deep well plates (DWP). In general, it can be stated that sample containers have a single receptacle for samples and sample carriers have a plurality of receptacles for samples.
- centrifuge container which can be suspended in a swing-out rotor, the centrifuge container having a special geometric design of its inner contour. More specifically, there are wall sections that define a receiving area for a substantially cuboid sample carrier, and there are wall sections that define a cylindrical receiving area for a centric sample bottle.
- the DE 20 202013 006 223 U1 shows a centrifuge container in which sample holders are set to hold sample tubes.
- the sample holder insert consists of a lower part and an upper part, which can optionally be connected to one another can be combined, whereby either only the lower part or the lower part and upper part can be combined with each other.
- the upper part is always placed on the lower part and, if necessary, locked so that short or long sample tubes can be held.
- the US 2016/250608 A1 shows a sample shaker in which there is a shaking device with a connecting piece onto which a carrier can be attached.
- the carrier has a central area in which there are receptacles for the vertical arrangements of sample containers. On the side of the central area there are four bowl-like receptacles for horizontally holding the corresponding sample containers.
- the object of the present invention is to eliminate these disadvantages.
- a centrifuge insert which is suitable not only for one type of sample container or sample carrier, but for at least two geometrically differently designed elements from the group of sample containers and sample carriers.
- One type should have a cross-section that is at least partially circular or oval and the other type should have a cross-section that is at least partially polygonal, in particular rectangular. This means that geometrically different elements can be centrifuged using the centrifuge insert with the centrifuge rotor.
- centrifuge insert could also be adapted to accommodate both different elements at the same time.
- This solution according to the invention ensures that the same centrifuge insert can still be used with geometrically different elements. This leads to space savings, especially in the laboratory. This also reduces the acquisition costs.
- the centrifuge insert according to the invention for receiving one or more elements from the group of sample containers and sample carriers in a centrifuge rotor, preferably designed as a swing-out rotor, of a centrifuge, in particular a laboratory centrifuge, is therefore characterized in that the centrifuge insert is adapted, at least a first element, which is at least partially has a circular or oval cross-section, and to optionally accommodate at least one second element, which has a polygonal cross-section at least in some areas.
- the polygonal cross section is preferably a rectangular cross section.
- the first element is a sample container, preferably a sample bottle, a sample tube, a tube, a reaction vessel, a centrifuge vessel, a bottle, a microreaction vessel or a cell culture bottle and in particular has a conical, flat or rounded bottom design .
- Standard sample containers can then be centrifuged using the centrifuge insert.
- the second element is a sample carrier, preferably a plate-shaped element, in particular in the form of a microplate, a microtiter plate, a PCR plate or a deepwell plate, with several receptacles for samples. Then you can use usual sample carriers Centrifuge with numerous holders for a variety of samples using the centrifuge insert.
- the centrifuge insert is adapted to accommodate the elements in a form-fitting manner. This means that the elements are held very securely even during centrifugation.
- the bottom design of receptacles of the centrifuge insert is adapted to the bottom design of sample containers and/or sample carriers to be accommodated and preferably has a flat, conical or rounded contour. This ensures that the sample containers or sample carriers are held particularly securely in the centrifuge insert.
- the centrifuge insert is designed to be modular with a base body and at least one supplementary part, the supplementary part being designed to be adjustable into the base body, in particular to be plugged in and/or set up on the base body, in particular to be plugged on, with a plug-in connection in particular between the base body and the supplementary part.
- the plug connection provides a force and/or positive connection, preferably has clamping properties. This means that the additional part is held particularly securely in the base body.
- the supplementary part has one or more receptacles for first elements.
- the supplementary part has an external geometry which, together with a corresponding internal geometry of the base body, forms one or more receptacles for first elements. This means that the centrifuge insert can be designed in a particularly variable manner and can still accommodate a maximum number of sample containers.
- the centrifuge insert is designed for lateral fixation of the first and/or the second elements.
- a lateral fixation can be achieved, for example, by depressions or grooves that are present at least in some areas and/or by webs or projections that exist at least in some areas and which correspond to corresponding elements, such as edges, of the first and second elements.
- This enables a very simple and yet effective recording, and by providing different grids of such depressions or webs and projections, differently sized first and second elements can also be accommodated.
- Such a second element can be designed as a sample carrier, for example as a microtiter plate. Such a second element can be at least partially enclosed laterally by the webs or projections and/or the depressions or grooves.
- the centrifuge insert is designed for vertical fixation of the first and/or second elements.
- a vertical fixation could be achieved, for example, by a snap and/or clip connection.
- the webs or projections could lock with a peripheral edge of the first or second element.
- the centrifuge insert is adapted, the first elements being inserted into corresponding receptacles of the centrifuge insert. Then the first elements, especially in the form of sample containers, are held very securely.
- the centrifuge insert is adapted to carry the second elements, the second elements preferably being fixed laterally in a form-fitting manner on a base surface. Then the second elements, especially in the form of sample carriers, are held very securely.
- the centrifuge insert has at least two guides arranged opposite each other, which are adapted to laterally support at least one supplementary part and/or at least one second element, preferably at least two supplementary parts or second elements stacked on top of each other. This ensures particularly secure recording.
- the guides are designed as handles for gripping the centrifuge insert.
- the centrifuge insert can then be transported particularly easily and manipulated in a centrifuge.
- the handles can also not fulfill a guiding function, i.e. they can only be intended for the handle function.
- the guides have locking connection elements which are adapted to provide a locking connection between the guides and the supplementary part and/or the second element. This makes the recording even better secured.
- a preferred embodiment of the centrifuge insert 10 according to the invention is shown in various views.
- FIG. 1 shows Fig. 1 the base body 12 of the centrifuge insert 10, which Fig. 2a and 2 B show two supplementary parts 14, 16 for this base body 12 and the Fig. 3 to 5 show various possible combinations of base body 12 and additional parts 14, 16 as well as the resulting applications.
- the base body 12 is essentially octagonal in cross section and has a central recess 18, which corresponds to six cylindrical segment-shaped recesses 20 which are equally spaced with respect to the circumference. Between two adjacent recesses 20 there is a vertically extending groove 22, which springs back outwards in relation to the central recess 18.
- a central recess 18 which corresponds to six cylindrical segment-shaped recesses 20 which are equally spaced with respect to the circumference.
- a vertically extending groove 22 which springs back outwards in relation to the central recess 18.
- handles 36 extending vertically upwards from the base surface 32 are provided, each of which comprises an approximately semi-cylindrical space 38 and has a top surface 40 at the top. This provides a handle 36 for gripping the centrifuge insert 10, which can be gripped particularly securely through the spaces 38.
- An edge 42 extends below the base surface 32, relative to which the outer walls 44 of the central recess 18 and the recesses 20 extend radially back.
- a first supplementary part 14 for the base body 12 is shown, which is essentially designed as a hollow cylinder with a wall 46 on which outwardly projecting ribs 48 extend at equal radial distances.
- the wall 46 has an outer circumference which is matched to the inner circumference of the central recess 18.
- the ribs 48 run downwards, becoming more pointed and becoming flatter on the other hand, and correspond to the grooves 22, which also become narrower and flatter away from the base surface 32.
- a large axial receptacle 59 is formed here, which can positively accommodate a circular cross section of a first element, for example a sample container.
- a second supplementary part 16 is shown for the base body 12, which is essentially star-shaped.
- the base body 12 In the middle there is an axial receptacle 60 and on the edge there are six cylindrical segment-shaped recesses 62 arranged at equal distances, with webs 64 extending between the recesses 62, on which ribs 66 in turn extend accordingly Fig. 2a are arranged.
- These ribs 66 can in turn fit snugly into the grooves 22 of the base body 12 in order to achieve a clamping effect when the supplementary part 16 is inserted into the base body 12 and thus hold the supplementary part 16 securely in the base body 12.
- the recesses 62 taper conically downwards 68, this shape corresponding to conical tapers 70 of the recesses 20.
- receptacles 72 are formed (cf. Fig. 4 ), which taper conically at the bottom and are therefore suitable for sample containers with a conically tapered bottom area.
- the axial receptacle 60 also has exactly the same geometry, so that there are seven identical receptacles 60, 72 here.
- the centrifuge insert 10a is designed as a combination of base body 12 and supplementary part 14 and is shown in the swing-out cup 110 of a swing-out rotor 108 of a laboratory centrifuge 100, which is only partially shown.
- This allows a single sample container 74, which is designed, for example, as a wide-neck bottle with a volume of 250 ml, to be centrifuged. Since the axial receptacle 59 has a width adapted to the circular cross section of the sample container 74, the sample container 74 is accommodated in the centrifuge insert 10a in a form-fitting and therefore secure manner.
- the centrifuge insert 10b is designed as a combination of base body 12 and supplementary part 16 and is shown in the swing-out cup 110 of a swing-out rotor 108 of a laboratory centrifuge 100, which is only partially shown.
- This allows individual sample containers 76, which are designed as sample tubes with a volume of 50 ml, to be centrifuged. Since the receptacles 60, 72 have a width adapted to the circular cross section of the sample containers 76, the sample containers 76 are also accommodated in a form-fitting and therefore secure manner in the centrifuge insert 10b.
- the centrifuge insert 10c is designed as a combination of base body 12 and supplementary part 14 and is shown in the swing-out cup 110 of a swing-out rotor 108 of a laboratory centrifuge 100, which is only partially shown.
- This allows, for example, individual sample carriers 78, which are designed as microtiter plates, to be centrifuged. Since the one from the webs 28, 30, 34, 34', 34" is adapted to the essentially rectangular base area 80 of the microtiter plate 78 and the edge 82 of the base area 80 can dip into the grooves 35, 35', 35" and the depressions 29, 31, the microtiter plate 78 is positively secured laterally to the centrifuge insert 10c and is therefore securely received (cf. also Fig. 9b , which shows a similar interaction). In this form of use, the supplementary part 16 could also be inserted or no supplementary part 14, 16 is inserted - the sample carrier 78 is always held securely laterally.
- the supplementary part 16 can also be inserted into the base body 12.
- no additional part 14, 16 needs to be inserted into the base body 12 at all, since the sample carrier 78 is supported on the base surface 32, so that the base body 12 alone can form the centrifuge insert 10.
- Fig. 6 the centrifuge insert 10a according to the invention is shown in cooperation with a laboratory centrifuge 100 in a perspective view.
- the laboratory centrifuge 100 has a housing 102 with a lid 104, which can close the sample space 106, in which a motor-driven (not shown) centrifuge rotor 108 is located.
- the centrifuge rotor 108 is designed as a swing-out rotor and has centrifuge cups 110, which can pivot away from the axis of rotation D, i.e. outwards, during centrifugation.
- the centrifuge inserts 10a according to the invention are accommodated in these centrifuge cups 110.
- a second embodiment of a centrifuge insert 200 not according to the invention is shown in various views.
- this centrifuge insert 200 differs from the centrifuge egg set 10 in that here the base body 202 with the handles 204 is designed without a central recess for receiving additional parts.
- nine identical receptacles 206 are arranged directly in the base body 202, which in principle correspond to the receptacles 60, 72 of the centrifuge insert 10a and are designed with the same inner diameter.
- vertically rising webs 210, 212, 214, 216 are provided on a base surface 208, which enclose a rectangular surface. At the same time there are recesses 211, 213 and grooves 215, 217, 218, 219.
- the handles 204 are not arranged symmetrically with respect to the webs 210, 212, but are slightly offset so that they can be arranged between two receptacles 206. However, this does not affect the grip function of these handles 204, since they are nevertheless arranged symmetrically with respect to the center of mass, which extends in the middle of the central receptacle 206.
- Fig. 8 the centrifuge insert 200 is shown in a first application of holding nine sample containers 220, which are designed as sample tubes with a capacity of 50 ml and can therefore be centrifuged in the laboratory centrifuge 100. Since the receptacles 206 have a width adapted to the circular cross section of the sample containers 220, the sample containers 220 are accommodated in the centrifuge insert 200 in a form-fitting and therefore secure manner.
- the centrifuge insert 200 is shown in two views in a second application of receiving a sample carrier 222, which is designed as a microtiter plate. Since in turn the rectangular area encompassed by the webs 210, 212, 214, 216 is adapted to the essentially rectangular base area 224 of the microtiter plate 222 and the edge 226 dips into the depressions 211, 213 and grooves 215, 217, 218, 219 , the microtiter plate 222 is positively secured laterally to the centrifuge insert 200 and is therefore securely received, as shown in particular Fig. 9b clearly shows. In addition, one could There is a clip connection or latching between the webs 210, 212, 214, 216 and the edge 226 in order to also fix the sample carrier 222 vertically.
- centrifuge insert 300 is shown in various views.
- Fig. 10 shows the base body 302 of the centrifuge insert 300, which differs from the base body 202 of the centrifuge insert 200 in that a larger number of receptacles 304 are provided in the base surface 306.
- These receptacles 304 are adapted to accommodate sample containers 308, which are in the form of reaction vessels with a volume of 5 ml, that is, since the receptacles 304 have a width adapted to the circular cross section of the sample containers 308, the sample containers 308 are form-fitting in the centrifuge insert 300 and therefore safely recorded (cf. in Fig. 11 corresponding first use case shown).
- the base surface 306 does not protrude so far compared to the bottom surface 310 of the base body 302. Therefore, with respect to a specific centrifuge cup 110 (cf. Fig. 6 ) the handles 312 can be made longer.
- this base body 302 also encompasses a rectangular surface in the form of webs 314, 316, 318, 318', 318", 318" as well as depressions 315, 317 and grooves 319, 319', 319".
- Fig. 10 also shows a supplementary part 320, which has a base surface 322 and walls 324 extending downwards from it, which provide numerous receptacles 326, the bottom surfaces of which are missing, so that the receptacles are designed as hollow cylinders with a constant cross-section over their entire length.
- These receptacles 326 are adapted to accommodate sample containers 328, which are in the form of sample bottles with a volume of 15 ml.
- Fig. 12 It can also be seen that the handles 312 engage with their outer contour in the indentations 330 of the supplementary part 320 in a form-fitting manner, so that the supplementary part is fixed laterally between the handles 312 and is therefore securely arranged on the base body 302 even in centrifugation mode.
- this design forms a threading and positioning aid for the supplementary part 320, so that the receptacles 326 of the supplementary part 320 can be positioned exactly over the receptacles 304 of the base body 302.
- the handles 312 here also form guides for the supplementary part 320.
- the supplementary part 320 has annular projections 336 on the receptacles 326 'at some points on its base surface 334, which engage in a positive and non-positive manner in corresponding recesses 304' of the base body 302.
- these receptacles 304 'of the base body 302 have an additional groove 337 into which the annular projections 336 can be inserted. This results in a further lateral fixation of the supplementary part 320 relative to the base body 302.
- the inner diameters of the receptacles 326, 326' of the supplementary part 320 and the receptacles 304, 304' of the base body 302 are identical, so that there is a continuously continuous receptacle 304, 304', 326, 326' results.
- annular projections 336 and groove 337 Due to this non-positive and positive connection of annular projections 336 and groove 337, an additional positive or positive connection with the handles 312 could also be dispensed with.
- the sample bottles 328 are relatively long. Due to the continuous design of the receptacles 304, 304 ', 326, 326' and the adaptation of their width to the circular cross section of the sample containers 328, the sample containers 328 are form-fitting in the centrifuge insert 300a, which is formed from the base body 302 and the supplementary part 320 and therefore safely recorded, whereby the sample containers 328 extend into the base body 302 and are held laterally by both the base body 302 and the supplementary part 320, as Fig. 12 shows.
- a sample carrier 338 which is designed as a microtiter plate, is arranged on the base body 302. Since in turn the rectangular area encompassed by the webs 314, 316, 318, 318 ', 318", 318' is adapted to the essentially rectangular base area 340 of the microtiter plate 338 and the edge 342 in the depressions 315, 317 and grooves 319 , 319 ', 319", the microtiter plate 338 is in turn secured laterally to the centrifuge insert 300 in a form-fitting manner and is therefore securely received.
- receptacles 400, 410, 420 of centrifuge inserts are shown purely schematically in cross-section in interaction with each recorded sample containers 402, 412, 422. It can be seen that the bottom designs of receptacles 400, 410, 420 and sample containers 402, 412, 422 are adapted to one another in such a way that there is a positive connection (the distance between the side walls is only drawn for better visibility), whereby the sample containers 402 , 412, 422 can be held particularly securely in the holders. Show it Fig. 14 a a flat, Fig. 14b a rounded and Fig. 14c a conical bottom design of the respective receptacle 400, 410, 420 and sample containers 402, 412, 422.
- centrifuge insert according to the invention can easily be applied to other sample containers and sample carriers, in particular those with a different geometry, including the floor design, and / or volume of the recording can be adjusted.
- the present invention provides a centrifuge insert 10, 10a, 10b, 10c, which can be used for different sample containers 74, 76 or sample carriers 78.
- This leads to space savings, especially in the laboratory.
- this reduces the acquisition costs and by reducing the manual effort, processes are accelerated and the laboratory throughput is increased.
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Description
Die vorliegende Erfindung betrifft einen Zentrifugeneinsatz zur Aufnahme ein oder mehrerer Elemente aus der Gruppe Probenbehälter und Probenträgern in einer Laborzentrifuge, der im Oberbegriff von Anspruch 1 angegeben ist.The present invention relates to a centrifuge insert for holding one or more elements from the group of sample containers and sample carriers in a laboratory centrifuge, which is specified in the preamble of
Zentrifugenrotoren werden in Zentrifugen, insbesondere Laborzentrifugen, dazu eingesetzt, um die Bestandteile von darin zentrifugierten Proben unter Ausnutzung der Massenträgheit zu trennen. Dabei werden zur Erzielung hoher Entmischungsraten immer höhere Rotationsgeschwindigkeiten eingesetzt. Laborzentrifugen sind dabei Zentrifugen, deren Rotoren bei vorzugsweise mindestens 3.000, bevorzugt mindestens 10.000, insbesondere mindestens 15.000 Umdrehungen pro Minute arbeiten und zumeist auf Tischen platziert werden. Um sie auf einem Arbeitstisch platzieren zu können, weisen sie insbesondere einen Formfaktor von weniger als 1 m × 1 m × 1 m auf, ihr Bauraum ist also beschränkt. Vorzugsweise ist dabei die Gerätetiefe auf max. 70 cm beschränkt.Centrifuge rotors are used in centrifuges, especially laboratory centrifuges, to separate the components of samples centrifuged therein using inertia. In order to achieve high segregation rates, ever higher rotation speeds are used. Laboratory centrifuges are centrifuges whose rotors work at preferably at least 3,000, preferably at least 10,000, in particular at least 15,000 revolutions per minute and are usually placed on tables. In order to be able to place them on a work table, they in particular have a form factor of less than 1 m × 1 m × 1 m, so their installation space is limited. The device depth is preferably limited to a maximum of 70 cm.
Zumeist ist vorgesehen, dass die Proben bei bestimmten Temperaturen zentrifugiert werden. Beispielsweise dürfen Proben, die Eiweiße und dgl. organische Substanzen enthalten, nicht überhitzt werden, so dass die Obergrenze für die Temperierung solcher Proben standardmäßig im Bereich von +40°C liegt. Andererseits werden bestimmte Proben standardmäßig im Bereich +4°C (die Anomalie des Wassers beginnt bei 3,98°C) gekühlt.It is usually intended that the samples be centrifuged at certain temperatures. For example, samples that contain proteins and similar organic substances must not be overheated, so that the upper limit for temperature control of such samples is standard in the range of +40°C. On the other hand, certain samples are cooled by default in the +4°C range (the water anomaly starts at 3.98°C).
Neben solchen vorbestimmten Höchsttemperaturen von beispielsweise ca. +40°C und Standarduntersuchungstemperaturen wie beispielsweise +4°C sind auch weitere Standarduntersuchungstemperaturen vorgesehen, wie beispielsweise bei +11°C, um bei dieser Temperatur zu prüfen, ob die Kälteanlage der Zentrifuge unterhalb Raumtemperatur geregelt läuft. Andererseits ist es aus Arbeitsschutzgründen notwendig, ein Anfassen von Elementen zu verhindern, die eine Temperatur von größer gleich +60°C aufweisen.In addition to such predetermined maximum temperatures of, for example, approximately +40 ° C and standard examination temperatures such as +4 ° C, other standard examination temperatures are also provided, such as +11 ° C, in order to check at this temperature whether the centrifuge's refrigeration system is running in a controlled manner below room temperature . On the other hand, for occupational safety reasons, it is necessary to prevent touching elements that have a temperature greater than or equal to +60°C.
Zur Temperierung können grundsätzlich aktive und passive Systeme verwendet werden. Aktive Kühlungssysteme besitzen einen Kältemittelkreislauf, der den Zentrifugenkessel temperiert, wodurch indirekt der Zentrifugenrotor und die darin aufgenommenen Probenbehälter gekühlt werden.In principle, active and passive systems can be used for temperature control. Active cooling systems have a refrigerant circuit that controls the temperature of the centrifuge bowl, thereby indirectly cooling the centrifuge rotor and the sample containers contained therein.
Passive Systeme basieren auf einer abluftunterstützten Kühlung bzw. Belüftung. Diese Luft wird direkt an dem Zentrifugenrotor vorbeigeführt, wodurch eine Temperierung erfolgt. Die Luft wird dabei durch Öffnungen in den Zentrifugenkessel gesaugt, wobei das Ansaugen selbständig durch die Drehung des Zentrifugenrotors erfolgt.Passive systems are based on exhaust air-supported cooling or ventilation. This air is led directly past the centrifuge rotor, which results in temperature control. The air is sucked into the centrifuge bowl through openings, with the suction taking place automatically through the rotation of the centrifuge rotor.
Die zu zentrifugierenden Proben werden in Probenbehältern oder Probenträger gelagert und diese Probenbehälter mittels des Zentrifugenrotors rotatorisch angetrieben. Dabei werden die Zentrifugenrotoren üblicherweise mittels einer senkrechten Antriebswelle, die von einem elektrischen Motor angetrieben wird, in Rotation versetzt. Es gibt verschiedene Zentrifugenrotoren, die je nach Anwendungszweck eingesetzt werden.The samples to be centrifuged are stored in sample containers or sample carriers and these sample containers are rotationally driven by the centrifuge rotor. The centrifuge rotors are usually set in rotation by means of a vertical drive shaft that is driven by an electric motor. There are different centrifuge rotors that are used depending on the application.
Ganz allgemein weisen solche Zentrifugenrotoren üblicherweise ein Unterteil und einen Deckel auf, wobei im geschlossenen Zustand des Deckels sich zwischen Unterteil und Deckel ein Innenraum ausbildet, in dem die Probenbehälter angeordnet werden können, um die Proben in einer geeigneten Zentrifuge zu zentrifugieren. Wenn die Probenbehälter oder Probenträger unter einem fest vorgegebenen Winkel in dem Zentrifugenrotor angeordnet sind, dann handelt es sich um einen sogenannten Festwinkelrotor. Wenn die Probenbehälter oder Probenträger dagegen in einer radialen Richtung in Bezug auf die Drehachse des Zentrifugenrotors verschwenkbar angeordnet sind, dann handelt es sich um einen Ausschwingrotor.In general, such centrifuge rotors usually have a lower part and a lid, with an interior space forming between the lower part and the lid in the closed state of the lid, in which the sample containers can be arranged in order to centrifuge the samples in a suitable centrifuge. If the sample containers or sample carriers are arranged at a fixed angle in the centrifuge rotor, then it is a so-called fixed-angle rotor. If, on the other hand, the sample containers or sample carriers are arranged to be pivotable in a radial direction with respect to the axis of rotation of the centrifuge rotor, then it is a swing-out rotor.
Die Zentrifugeneinsätze werden verwendet, um die Probenbehälter oder Probenträger in den Rotoren der Zentrifugen anordnen und zentrifugieren zu können.The centrifuge inserts are used to arrange and centrifuge the sample containers or sample carriers in the rotors of the centrifuges.
Beispielsweise sind Ausschwingrotoren bekannt, die einen verschwenkbaren Zentrifugenbecher aufweisen, in den ein solcher Zentrifugeneinsatz einstellbar ist.For example, swing-bucket rotors are known which have a pivotable centrifuge cup into which such a centrifuge insert can be adjusted.
Es sind viele verschiedene Arten von Probenbehältern bekannt, wie beispielsweise Probenflaschen, Probenbecher, Probenröhrchen, Tubes, Reaktionsgefäße, Zentrifugengefäße, Flaschen, Mikroreaktionsgefäße oder Zellkulturflaschen, die auch noch in unterschiedlichen Größen vorliegen können. Diese Probenbehälter können auch unterschiedliche Bodengestaltungen aufweisen, beispielsweise flach, konisch oder abgerundet. Außerdem gibt es auch unterschiedliche Arten von Probenträgern, wie beispielsweise Mikroplatten, Mikrotiter-Platten (MTP), PCR-Platten und Deepwellplatten (DWP). Allgemein lässt sich feststellen, dass Probenbehälter eine einzelne Aufnahme für Proben und Probenträger eine Mehrzahl an Aufnahmen für Proben aufweisen.Many different types of sample containers are known, such as sample bottles, sample cups, sample tubes, tubes, reaction vessels, centrifuge vessels, bottles, microreaction vessels or cell culture bottles, which can also be available in different sizes. These sample containers can also have different bottom designs, for example flat, conical or rounded. There are also different types of sample carriers, such as microplates, microtiter plates (MTP), PCR plates and deep well plates (DWP). In general, it can be stated that sample containers have a single receptacle for samples and sample carriers have a plurality of receptacles for samples.
Nachteilig daran ist, dass für jeden Probenbehälter bzw. jeden Probenträger eigene Zentrifugeneinsätze erforderlich sind. Dadurch müssen die Anwender viele verschiedene Zentrifugeneinsätze vorrätig halten, was zum einen kostenintensiv in Bezug auf die Anschaffung und zum anderen auch platzintensiv in Bezug auf deren Lagerung ist.The disadvantage of this is that separate centrifuge inserts are required for each sample container or sample carrier. This means that users have to keep many different centrifuge inserts in stock, which is both costly in terms of purchasing and also space-intensive in terms of storing them.
Aus der
Die
Die
Die Aufgabe der vorliegenden Erfindung besteht darin, diese Nachteile zu beseitigen.The object of the present invention is to eliminate these disadvantages.
Diese Aufgabe wird gelöst mit dem erfindungsgemäßen Zentrifugeneinsatz nach Anspruch 1. Vorteilhafte Weiterbildungen sind in den Unteransprüchen und in der nachfolgenden Beschreibung zusammen mit den Figuren angegeben.This object is achieved with the centrifuge insert according to the invention according to
Erfinderseits wurde erkannt, dass diese Aufgabe in überraschender Art und Weise dadurch besonders einfach gelöst werden kann, wenn ein Zentrifugeneinsatz bereitgestellt wird, der nicht nur für eine Art Probenbehälter bzw. Probenträger geeignet ist, sondern für mindestens zwei geometrisch unterschiedlich ausgebildete Elemente aus der Gruppe Probenbehälter und Probenträger. Dabei soll die eine Art einen zumindest bereichsweise kreisförmigen oder ovalen Querschnitt und die andere Art einen zumindest bereichsweise vieleckigen, insbesondere rechteckigen Querschnitt aufweisen. Damit können wahlweise geometrisch unterschiedliche Elemente durch den Zentrifugeneinsatz mit dem Zentrifugenrotor zentrifugiert werden.On the part of the inventor, it was recognized that this task can be solved particularly easily in a surprising manner if a centrifuge insert is provided which is suitable not only for one type of sample container or sample carrier, but for at least two geometrically differently designed elements from the group of sample containers and sample carriers. One type should have a cross-section that is at least partially circular or oval and the other type should have a cross-section that is at least partially polygonal, in particular rectangular. This means that geometrically different elements can be centrifuged using the centrifuge insert with the centrifuge rotor.
Hierzu können Ergänzungsteile verwendet werden. Dies ist allerdings nicht zwingend notwendig. Es kann sich um eine alternative Anordnungsmöglichkeit der unterschiedlichen Elemente handeln, allerdings könnte der Zentrifugeneinsatz auch angepasst sein, zugleich beide unterschiedliche Elemente aufzunehmen.Additional parts can be used for this. However, this is not absolutely necessary. This may be an alternative arrangement option for the different elements, but the centrifuge insert could also be adapted to accommodate both different elements at the same time.
Durch diese erfindungsgemäße Lösung wird erreicht, dass bei geometrisch unterschiedlichen Elementen dennoch derselbe Zentrifugeneinsatz verwendet werden kann. Dies führt zu einer Platzersparnis, insbesondere im Labor. Außerdem werden dadurch die Anschaffungskosten reduziert.This solution according to the invention ensures that the same centrifuge insert can still be used with geometrically different elements. This leads to space savings, especially in the laboratory. This also reduces the acquisition costs.
Der erfindungsgemäße Zentrifugeneinsatz zur Aufnahme ein oder mehrerer Elemente aus der Gruppe Probenbehälter und Probenträger in einem, bevorzugt als Ausschwingrotor ausgebildeten Zentrifugenrotor einer Zentrifuge, insbesondere einer Laborzentrifuge, zeichnet sich daher dadurch aus, dass der Zentrifugeneinsatz angepasst ist, zumindest ein erstes Element, das zumindest bereichsweise einen kreisförmigen oder ovalen Querschnitt aufweist, und zumindest ein zweites Element, das zumindest bereichsweise einen vieleckigen Querschnitt aufweist, wahlweise aufzunehmen. Bei dem vieleckigen Querschnitt handelt es sich bevorzugt um einen rechteckigen Querschnitt.The centrifuge insert according to the invention for receiving one or more elements from the group of sample containers and sample carriers in a centrifuge rotor, preferably designed as a swing-out rotor, of a centrifuge, in particular a laboratory centrifuge, is therefore characterized in that the centrifuge insert is adapted, at least a first element, which is at least partially has a circular or oval cross-section, and to optionally accommodate at least one second element, which has a polygonal cross-section at least in some areas. The polygonal cross section is preferably a rectangular cross section.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass das erste Element ein Probenbehälter, bevorzugt eine Probenflasche, ein Probenröhrchen, ein Tube, ein Reaktionsgefäß, ein Zentrifugengefäß, eine Flasche, ein Mikroreaktionsgefäß oder eine Zellkulturflasche ist und insbesondere einen konisch, flach oder abgerundet ausgebildete Bodengestaltung aufweist. Dann lassen sich übliche Probenbehälter mit dem Zentrifugeneinsatz zentrifugieren.In an advantageous development it is provided that the first element is a sample container, preferably a sample bottle, a sample tube, a tube, a reaction vessel, a centrifuge vessel, a bottle, a microreaction vessel or a cell culture bottle and in particular has a conical, flat or rounded bottom design . Standard sample containers can then be centrifuged using the centrifuge insert.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass das zweite Element ein Probenträger, bevorzugt ein plattenförmiges Element, insbesondere in der Form einer Mikroplatte, einer Mikrotiter-Plate, einer PCR-Platte oder einer Deepwell-Platte, mit mehreren Aufnahmen für Proben ist. Dann lassen sich übliche Probenträger mit zahlreichen Aufnahmen für eine Vielzahl von Proben mit dem Zentrifugeneinsatz zentrifugieren.In an advantageous development it is provided that the second element is a sample carrier, preferably a plate-shaped element, in particular in the form of a microplate, a microtiter plate, a PCR plate or a deepwell plate, with several receptacles for samples. Then you can use usual sample carriers Centrifuge with numerous holders for a variety of samples using the centrifuge insert.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass der Zentrifugeneinsatz angepasst ist, die Elemente formschlüssig aufzunehmen. Dadurch werden die Elemente sehr sicher auch im Zentrifugierbetrieb gehalten.In an advantageous development it is provided that the centrifuge insert is adapted to accommodate the elements in a form-fitting manner. This means that the elements are held very securely even during centrifugation.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass die Bodengestaltung von Aufnahmen des Zentrifugeneinsatzes an die Bodengestaltung aufzunehmender Probenbehälter und/oder Probenträger angepasst ist und bevorzugt eine flache, konische oder abgerundete Kontur aufweist. Dadurch besteht eine besonders sichere Halterung der Probenbehälter bzw. Probenträger in dem Zentrifugeneinsatz.In an advantageous development it is provided that the bottom design of receptacles of the centrifuge insert is adapted to the bottom design of sample containers and/or sample carriers to be accommodated and preferably has a flat, conical or rounded contour. This ensures that the sample containers or sample carriers are held particularly securely in the centrifuge insert.
Erfindungsgemäß ist vorgesehen, dass der Zentrifugeneinsatz modular mit einem Grundkörper und zumindest einem Ergänzungsteil ausgebildet ist, wobei das Ergänzungsteil in den Grundkörper einstellbar, insbesondere einsteckbar und/oder auf den Grundkörper aufstellbar, insbesondere aufsteckbar ausgebildet ist, wobei zwischen Grundkörper und Ergänzungsteil insbesondere eine Steckverbindung, vorzugsweise in Form einer Nut-Feder-Verbindung ausbildbar ist. Dadurch lässt sich der Zentrifugeneinsatz sehr leicht an bestimmte Erfordernisse einzelner Probenbehälter oder Probenträger anpassen. Dennoch bleibt die Platz- und Kostenersparnis gewahrt, weil nur ein Grundkörper und ein oder mehrere Ergänzungsteile angeschafft und vorrätig gehalten werden müssen.According to the invention, it is provided that the centrifuge insert is designed to be modular with a base body and at least one supplementary part, the supplementary part being designed to be adjustable into the base body, in particular to be plugged in and/or set up on the base body, in particular to be plugged on, with a plug-in connection in particular between the base body and the supplementary part. can preferably be formed in the form of a tongue-and-groove connection. This means that the centrifuge insert can be easily adapted to the specific requirements of individual sample containers or sample carriers. Nevertheless, the space and cost savings are maintained because only a basic body and one or more additional parts have to be purchased and kept in stock.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass die Steckverbindung einen Kraft- und/oder Formschluss bereitstellt, bevorzugt Klemmeigenschaften aufweist. Dadurch wird das Ergänzungsteil besonders sicher in dem Grundkörper gehalten. Beispielsweise könnte eine Nur-Feder-Verbindung bestehen, bei der die Feder sich in Bezug auf die Einsteckrichtung konisch erweitert.In an advantageous development it is provided that the plug connection provides a force and/or positive connection, preferably has clamping properties. This means that the additional part is held particularly securely in the base body. For example, there could be a spring-only connection in which the spring expands conically in relation to the insertion direction.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass das Ergänzungsteil ein oder mehrere Aufnahmen für erste Elemente aufweist. Erfindungsgemäß ist vorgesehen dass das Ergänzungsteil eine Außengeometrie besitzt, die zusammen mit einer korrespondierenden Innengeometrie des Grundkörpers ein oder mehrere Aufnahmen für erste Elemente bildet. Dadurch ist der Zentrifugeneinsatz besonders variabel gestaltbar und kann insbesondere dennoch jeweils eine maximale Anzahl von Probenbehältern aufnehmen.In an advantageous development it is provided that the supplementary part has one or more receptacles for first elements. According to the invention it is provided that the supplementary part has an external geometry which, together with a corresponding internal geometry of the base body, forms one or more receptacles for first elements. This means that the centrifuge insert can be designed in a particularly variable manner and can still accommodate a maximum number of sample containers.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass der Zentrifugeneinsatz zur lateralen Fixierung der ersten und/oder der zweiten Elemente ausgebildet ist. Eine solche laterale Fixierung kann beispielsweise durch zumindest bereichsweise vorliegende Vertiefungen oder Nuten und/oder durch zumindest bereichsweise bestehende Stege oder Vorsprünge erreicht werden, die mit entsprechenden Elementen, wie beispielsweise Rändern, der ersten bzw. der zweiten Elemente korrespondieren. Dadurch wird eine sehr einfache und dennoch wirksame Aufnahme ermöglicht, wobei durch Vorsehung von verschiedenen Rastern solcher Vertiefungen bzw. Stege und Vorsprünge auch unterschiedlich dimensionierte erste bzw. zweite Elemente aufgenommen werden können. Ein solches zweites Element kann als Probenträger, beispielsweise als eine Mikrotiter-Platte ausgebildet sein. Ein solches zweites Element kann durch die Stege bzw. Vorsprünge und/oder die Vertiefungen bzw. Nuten seitlich zumindest teilweise umfasst werden.In an advantageous development it is provided that the centrifuge insert is designed for lateral fixation of the first and/or the second elements. Such a lateral fixation can be achieved, for example, by depressions or grooves that are present at least in some areas and/or by webs or projections that exist at least in some areas and which correspond to corresponding elements, such as edges, of the first and second elements. This enables a very simple and yet effective recording, and by providing different grids of such depressions or webs and projections, differently sized first and second elements can also be accommodated. Such a second element can be designed as a sample carrier, for example as a microtiter plate. Such a second element can be at least partially enclosed laterally by the webs or projections and/or the depressions or grooves.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass der Zentrifugeneinsatz zur vertikalen Fixierung der ersten und/oder zweiten Elemente ausgebildet ist. Eine solche vertikale Fixierung könnte beispielsweise durch eine Rast- und/oder Clipverbindung erreicht werden. Beispielsweise könnten die Stege oder Vorsprünge mit einem umlaufenden Rand des ersten bzw. zweiten Elements verrasten.In an advantageous development it is provided that the centrifuge insert is designed for vertical fixation of the first and/or second elements. Such a vertical fixation could be achieved, for example, by a snap and/or clip connection. For example, the webs or projections could lock with a peripheral edge of the first or second element.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass der Zentrifugeneinsatz angepasst ist, die ersten Elemente durch Einstecken in entsprechende Aufnahmen des Zentrifugeneinsatzes aufzunehmen. Dann sind die ersten Elemente, insbesondere in Form von Probenbehältern sehr sicher gehalten.In an advantageous development it is provided that the centrifuge insert is adapted, the first elements being inserted into corresponding receptacles of the centrifuge insert. Then the first elements, especially in the form of sample containers, are held very securely.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass der Zentrifugeneinsatz angepasst ist, die zweiten Elemente zu tragen, wobei die zweiten Elemente vorzugsweise auf einer Basisfläche formschlüssig lateral festgelegt sind. Dann sind die zweiten Elemente, insbesondere in Form von Probenträgern sehr sicher gehalten.In an advantageous development it is provided that the centrifuge insert is adapted to carry the second elements, the second elements preferably being fixed laterally in a form-fitting manner on a base surface. Then the second elements, especially in the form of sample carriers, are held very securely.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass der Zentrifugeneinsatz zumindest zwei gegenüberliegend angeordnete Führungen aufweist, die angepasst sind, zumindest ein Ergänzungsteil und/oder zumindest ein zweites Element, bevorzugt zumindest zwei aufeinander gestapelte Ergänzungsteile bzw. zweite Elemente lateral abzustützen. Dadurch besteht eine besonders sichere Aufnahme.In an advantageous development, it is provided that the centrifuge insert has at least two guides arranged opposite each other, which are adapted to laterally support at least one supplementary part and/or at least one second element, preferably at least two supplementary parts or second elements stacked on top of each other. This ensures particularly secure recording.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass die Führungen als Griffe zum Ergreifen des Zentrifugeneinsatzes ausgebildet sind. Dann lässt sich der Zentrifugeneinsatz besonders leicht transportieren und in einer Zentrifuge manipulieren. Die Griffe können alternativ aber auch keine Führungsfunktion erfüllen, also nur für die Grifffunktion vorgesehen sein.In an advantageous development it is provided that the guides are designed as handles for gripping the centrifuge insert. The centrifuge insert can then be transported particularly easily and manipulated in a centrifuge. Alternatively, the handles can also not fulfill a guiding function, i.e. they can only be intended for the handle function.
In einer vorteilhaften Weiterbildung ist vorgesehen, dass die Führungen Rastverbi n-dungselemente aufweisen, die angepasst sind, eine Rastverbindung zwischen den Führungen und dem Ergänzungsteil und/oder dem zweiten Element bereitzustellen. Dadurch wird die Aufnahme noch besser gesichert.In an advantageous development it is provided that the guides have locking connection elements which are adapted to provide a locking connection between the guides and the supplementary part and/or the second element. This makes the recording even better secured.
Die Merkmale und weitere Vorteile der vorliegenden Erfindung werden im Folgenden anhand der Beschreibung bevorzugter Ausführungsbeispiele im Zusammenhang mit den Figuren deutlich werden. Dabei zeigen rein schematisch:
- Fig. 1
- den Grundkörper des erfindungsgemäßen Zentrifugeneinsatzes nach einer bevorzugten Ausgestaltung in einer perspektivischen Ansicht,
- Fig. 2a und 2b
- zwei Ergänzungsteile für den Grundkörper des erfindungsgemäßen Zentrifugeneinsatzes nach
Fig. 1 in perspektivischen Ansichten, - Fig. 3
- den erfindungsgemäßen Zentrifugeneinsatz nach
Fig. 1 in einer ersten Anwendung in einer perspektivischen Ansicht, - Fig. 4
- den erfindungsgemäßen Zentrifugeneinsatz nach
Fig. 1 in einer zweiten Anwendung in einer perspektivischen Ansicht, - Fig. 5
- den erfindungsgemäßen Zentrifugeneinsatz nach
Fig. 1 in einer dritten Anwendung in einer perspektivischen Ansicht, - Fig. 6
- eine Laborzentrifuge mit Zentrifugenrotor und darin angeordnetem Zentrifugeneinsatz in einer perspektivischen Ansicht,
- Fig. 7
- den erfindungsgemäßen Zentrifugeneinsatz gemäß einer nicht erfindungsgemäßen zweiten Ausgestaltung in einer perspektivischen Ansicht,
- Fig. 8
- den Zentrifugeneinsatz nach
Fig. 7 in einer ersten Anwendung in einer perspektivischen Ansicht, - Fig. 9a, 9b
- den Zentrifugeneinsatz nach
Fig. 7 in einer zweiten Anwendung in einer perspektivischen Ansicht und einer Seitenansicht, - Fig. 10
- den Grundkörper und das Ergänzungsteil des Zentrifugeneinsatzes nach einer dritten Ausgestaltung in einer perspektivischen Ansicht,
- Fig. 11
- den Zentrifugeneinsatz nach
Fig. 10 in einer ersten Anwendung in einer perspektivischen Ansicht, - Fig. 12
- den Zentrifugeneinsatz nach
Fig. 10 in einer zweiten Anwendung in einer perspektivischen Ansicht, - Fig. 13
- den Zentrifugeneinsatz nach
Fig. 10 in einer dritten Anwendung in einer perspektivischen Ansicht und - Fig. 14a, b, c
- die Aufnahmen erfindungsgemäßer Zentrifugeneinsätze im Zusammenwirken mit aufgenommenen Probenbehältern im Querschnitt.
- Fig. 1
- the base body of the centrifuge insert according to the invention according to a preferred embodiment in a perspective view,
- Figs. 2a and 2b
- two additional parts for the base body of the centrifuge insert according to the invention
Fig. 1 in perspective views, - Fig. 3
- the centrifuge insert according to the invention
Fig. 1 in a first application in a perspective view, - Fig. 4
- the centrifuge insert according to the invention
Fig. 1 in a second application in a perspective view, - Fig. 5
- the centrifuge insert according to the invention
Fig. 1 in a third application in a perspective view, - Fig. 6
- a laboratory centrifuge with a centrifuge rotor and a centrifuge insert arranged therein in a perspective view,
- Fig. 7
- the centrifuge insert according to the invention according to a second embodiment not according to the invention in a perspective view,
- Fig. 8
- the use of the centrifuge
Fig. 7 in a first application in a perspective view, - 9a, 9b
- the use of the centrifuge
Fig. 7 in a second application in a perspective view and a side view, - Fig. 10
- the base body and the additional part of the centrifuge insert according to a third embodiment in a perspective view,
- Fig. 11
- the use of the centrifuge
Fig. 10 in a first application in a perspective view, - Fig. 12
- the use of the centrifuge
Fig. 10 in a second application in a perspective view, - Fig. 13
- the use of the centrifuge
Fig. 10 in a third application in a perspective view and - Fig. 14a, b, c
- the recordings of centrifuge inserts according to the invention in cooperation with sample containers in cross section.
In den
Genauer gesagt zeigt
Es ist zu erkennen, dass der Grundkörper 12 im Wesentlichen oktogonal im Querschnitt ausgebildet ist und eine zentrale Ausnehmung 18 aufweist, die mit sechs in Bezug auf den Umfang gleich beabstandeten zylindersegmentförmigen Ausnehmungen 20 korrespondiert. Zwischen jeweils zwei benachbarten Ausnehmungen 20 befindet sich eine vertikal verlaufende Nut 22, die in Bezug auf die zentrale Ausnehmung 18 nach außen zurückspringt. Anstelle eines oktogonalen kann auch ein anders gestalteter Querschnitt vorliegen.It can be seen that the
An den beiden Außenseiten 24, 26 des oktogonalen Querschnitts befinden sich Stege 28, 30, die gegenüber der Basisfläche 32 des Grundkörpers 12 vertikal nach oben auskragen. Außerdem befinden sich auf der Basisfläche 32 gegenüberliegend angeordnete Stege 34, 34', 34", wobei die Stege 28, 30, 34, 34', 34" zwischen sich einen rechteckigen Bereich umfassen, der formschlüssig einen rechteckigen Querschnitt eines zweiten Elements, bspw. eines Probenträgers, aufnehmen kann. Zugleich befinden sich vor den Stegen 28, 30 in Bezug auf die Basisfläche 32 Vertiefungen 29, 31 und vor den Stegen 34, 34', 34" Nuten 35, 35', 35".On the two
Schließlich sind vertikal von der Basisfläche 32 nach oben sich erstreckende Griffe 36 vorgesehen, die jeweils einen etwa halbzylindrischen Raum 38 umfassen und oben eine Deckfläche 40 aufweisen. Dadurch wird ein Griff 36 zum Ergreifen des Zentrifugeneinsatzes 10 bereitgestellt, der durch die Räume 38 besonders sicher ergriffen werden kann.Finally, handles 36 extending vertically upwards from the
Unterhalb der Basisfläche 32 erstreckt sich ein Rand 42, gegenüber dem radial zurückversetzt die Außenwände 44 der zentralen Ausnehmung 18 und der Ausnehmungen 20 verlaufen.An
In
Durch die Ausbildung als Hohlzylinder des Ergänzungsteils 14 wird hier eine große axiale Aufnahme 59 gebildet, die formschlüssig einen kreisförmigen Querschnitt eines ersten Elements, bspw. eines Probenbehälters, aufnehmen kann.By designing the
In
Weiterhin ist zu erkennen, dass die Ausnehmungen 62 sich nach unten hin konisch verjüngen 68, wobei diese Formgebung mit konischen Verjüngungen 70 der Ausnehmungen 20 korrespondiert. Nach dem Einstecken des Ergänzungsteils 16 in den Grundkörper 12 bilden sich dadurch Aufnahmen 72 (vgl.
In
In
In
Alternativ zu dieser Ausgestaltung kann auch das Ergänzungsteil 16 in den Grundkörper 12 eingesetzt sein. Es muss allerdings auch überhaupt kein Ergänzungsteil 14, 16 in den Grundkörper 12 eingesetzt sein, da sich der Probenträger 78 auf der Basisfläche 32 abstützt, so dass der Grundkörper 12 allein schon den Zentrifugeneinsatz 10 bilden kann.As an alternative to this embodiment, the
In
In den
Es ist zu erkennen, dass sich dieser Zentrifugeneinsatz 200 von dem Zentrifugenei n-satz 10 dadurch unterscheidet, dass hier der Grundkörper 202 mit den Griffen 204 ohne eine zentrale Ausnehmung zur Aufnahme von Ergänzungsteilen ausgebildet ist.It can be seen that this
Stattdessen sind neun identische Aufnahmen 206 direkt im Grundkörper 202 angeordnet, die prinzipiell den Aufnahmen 60, 72 des Zentrifugeneinsatzes 10a entsprechen, und mit gleichem Innendurchmesser ausgebildet sind.Instead, nine
Auch hier sind wieder auf einer Basisfläche 208 vertikal nach oben aufstrebende Stege 210, 212, 214, 216 vorgesehen, die eine Rechteckfläche umschließen. Zugleich bestehen wieder die Vertiefungen 211, 213 und Nuten 215, 217, 218, 219.Here too, vertically rising
Die Griffe 204 sind hier im Gegensatz zum Zentrifugeneinsatz 10 nicht symmetrisch in Bezug auf die Stege 210, 212 angeordnet, sondern leicht versetzt, damit sie zwischen zwei Aufnahmen 206 angeordnet werden können. Dadurch wird aber die Grifffunktion dieser Griffe 204 nicht beeinträchtigt, da sie dennoch in Bezug auf den Massenschwerpunkt, der sich in der Mitte der zentralen Aufnahme 206 erstreckt, symmetrisch angeordnet sind.In contrast to the
In
In den
In den
Weiterhin ist zu erkennen, dass die Basisfläche 306 gegenüber der Bodenfläche 310 des Grundkörpers 302 nicht so weit hochsteht. Daher können in Bezug auf einen bestimmten Zentrifugenbecher 110 (vgl.
Ansonsten besteht auch bei diesem Grundkörper 302 eine Umfassung einer rechteckigen Fläche in Form von Stegen 314, 316, 318, 318', 318", 318‴ sowie Vertiefungen 315, 317 und Nuten 319, 319', 319".Otherwise, this
In
Außerdem weist das Ergänzungsteil 320 an einigen Stellen seiner Bodenfläche 334 ringförmige Vorsprünge 336 an den Aufnahmen 326' auf, die in entsprechende Ausnehmungen 304' des Grundkörpers 302 form- und kraftschlüssig eingreifen. Dazu weisen diese Aufnahmen 304' des Grundkörpers 302 eine zusätzliche Kehlung 337 auf, in die die ringförmigen Vorsprünge 336 einsteckbar sind. Dadurch erfolgt eine weitere laterale Festlegung des Ergänzungsteils 320 gegenüber dem Grundkörper 302. Die Innendurchmesser der Aufnahmen 326, 326' des Ergänzungsteils 320 und der Aufnahmen 304, 304' des Grundkörpers 302 sind identisch, so dass sich eine kontinuierlich durchgehende Aufnahme 304, 304',326, 326' ergibt.In addition, the
Aufgrund dieser kraft- und formschlüssigen Verbindung von ringförmigen Vorsprüngen 336 und Kehlung 337 könnte auch auf einen zusätzlichen Kraft- bzw. Formschluss mit den Griffen 312 verzichtet werden.Due to this non-positive and positive connection of
Die Probenflaschen 328 weisen eine relativ große Länge auf. Durch die durchgehende Ausbildung der Aufnahmen 304, 304',326, 326' und der Anpassung deren Weite an den kreisförmigen Querschnitt der Probenbehälter 328, sind die Probenbehälter 328 in dem Zentrifugeneinsatz 300a, der aus dem Grundkörper 302 und dem Ergänzungsteil 320 gebildet wird, formschlüssig und damit sicher aufgenommen, wobei die Probenbehälter 328 bis in den Grundkörper 302 durchreichen und lateral sowohl von dem Grundkörper 302 als auch von dem Ergänzungsteil 320 gehalten werden, wie
In
In den
Auch wenn im Rahmen der vorstehenden Beschreibung bestimmte Probenbehälter 74, 76, 220, 308, 328, 402, 412, 422 und Probenträger 78, 222, 338 beschrieben wurden, kann der erfindungsgemäße Zentrifugeneinsatz ohne weiteres auch an andere Probenbehälter und Probenträger, insbesondere solche mit einer anderen Geometrie, einschließlich der Bodengestaltung, und/oder Volumen der Aufnahme angepasst werden.Even if
Aus der vorstehenden Darstellung ist deutlich geworden, dass mit der vorliegenden Erfindung ein Zentrifugeneinsatz 10, 10a, 10b, 10c bereitgestellt wird, der für unterschiedliche Probenbehälter 74, 76 oder Probenträger 78 verwendet werden kann. Dies führt zu einer Platzersparnis, insbesondere im Labor. Außerdem werden dadurch die Anschaffungskosten reduziert und durch eine Reduzierung des manuellen Aufwandes werden Handlungsabläufe beschleunigt und dadurch der Labordurchsatz erhöht.From the above illustration it is clear that the present invention provides a
- 10, 10a, 10b, 10c10, 10a, 10b, 10c
- erste bevorzugte Ausgestaltung des erfindungsgemäßen Zentrifugeneinsatzesfirst preferred embodiment of the centrifuge insert according to the invention
- 1212
- Grundkörper des ZentrifugeneinsatzesBasic body of the centrifuge insert
- 14, 1614, 16
- Ergänzungsteile für den GrundkörperAdditional parts for the basic body
- 1818
- zentrale Ausnehmungcentral recess
- 2020
- zylindersegmentförmige Ausnehmungencylindrical segment-shaped recesses
- 2222
- vertikal verlaufende Nutvertical groove
- 24, 2624, 26
- Außenseitenoutsides
- 28, 3028, 30
- StegeBridges
- 3232
- Basisfläche GrundkörpersBase area of the base body
- 34, 34', 34"34, 34', 34"
- StegeBridges
- 29, 3129, 31
- Vertiefungendepressions
- 35, 35', 35"35, 35', 35"
- Nutengrooves
- 3636
- GriffeHandles
- 3838
- halbzylindrischer Raumsemi-cylindrical space
- 4040
-
Deckfläche 40
Cover area 40 - 4242
- Randedge
- 4444
-
Außenwände der zentralen Ausnehmung 18 und der Ausnehmungen 20Outer walls of the central recess 18 and the
recesses 20 - 4646
-
Wand des Ergänzungsteils 14Wall of
supplementary part 14 - 4848
-
nach außen vorspringende Rippen 48outwardly projecting
ribs 48 - 5959
- axiale Aufnahmeaxial recording
- 6060
-
axiale Aufnahme des Ergänzungsteils 16axial recording of the
additional part 16 - 6262
- zylindersegmentförmige Ausnehmungencylindrical segment-shaped recesses
- 6464
- StegeBridges
- 6666
- RippenRibs
- 6868
-
konische Verjüngung der Ausnehmung 62conical taper of the
recess 62 - 7070
-
konische Verjüngung der Ausnehmung 20conical taper of the
recess 20 - 7272
- AufnahmenRecordings
- 7474
- Probenbehälter, Weithalsflasche 250 mlSample container, wide-mouth bottle 250 ml
- 7676
- Probenbehälter, Probenröhrchen 50 mlSample container, sample tube 50 ml
- 7878
- Probenträger, Mikrotiter-PlatteSample carrier, microtiter plate
- 8080
-
Grundfläche der Mikrotiter-Platte 78Base area of the
microtiter plate 78 - 8282
-
Rand der Grundfläche 80Edge of the
base area 80 - 100100
- LaborzentrifugeLaboratory centrifuge
- 102102
- GehäuseHousing
- 104104
- DeckelLid
- 106106
- ProbenraumRehearsal room
- 108108
- Zentrifugenrotor, AusschwingrotorCentrifuge rotor, swing-bucket rotor
- 110110
- ZentrifugenbecherCentrifuge beaker
- 200200
- zweite bevorzugte Ausgestaltung des erfindungsgemäßen Zentrifugeneinsatzessecond preferred embodiment of the centrifuge insert according to the invention
- 202202
-
Grundkörper des Zentrifugeneinsatzes 200Basic body of the
centrifuge insert 200 - 204204
- GriffeHandles
- 206206
- AufnahmenRecordings
- 208208
- Basisflächebase area
- 210, 212, 214, 216210, 212, 214, 216
- StegeBridges
- 211, 213211, 213
- Vertiefungendepressions
- 215, 217, 218, 219215, 217, 218, 219
- Nutengrooves
- 220220
- Probenbehälter, Probenröhrchen 50 mlSample container, sample tube 50 ml
- 222222
- Probenträgers, Mikrotiter-PlatteSample carrier, microtiter plate
- 224224
-
Grundfläche der Mikrotiter-Platte 222Base area of the
microtiter plate 222 - 226226
-
Rand der Grundfläche 224Edge of the
base area 224 - 300300
- dritte bevorzugte Ausgestaltung des erfindungsgemäßen Zentrifugeneinsatzesthird preferred embodiment of the centrifuge insert according to the invention
- 302302
-
Grundkörper des Zentrifugeneinsatzes 300Basic body of the
centrifuge insert 300 - 304304
- AufnahmenRecordings
- 304'304'
-
Aufnahme mit ringförmigem Vorsprung 336Recording with
annular projection 336 - 306306
- Basisflächebase area
- 308308
- Probenbehälter, Reaktionsgefäß 5 mlSample container, reaction vessel 5 ml
- 310310
-
Bodenfläche des Grundkörpers 302Bottom area of the
base body 302 - 312312
- Führungen, GriffeGuides, handles
- 314, 316, 318, 318', 318"314, 316, 318, 318', 318"
- StegeBridges
- 315, 317315, 317
- Vertiefungendepressions
- 319, 319', 319"319, 319', 319"
- Nutengrooves
- 320320
- ErgänzungsteilSupplementary part
- 322322
- Basisflächebase area
- 324324
- Wandungenwalls
- 326326
- AufnahmenRecordings
- 326'326'
- Aufnahmen mit Kehlung 327Recordings with throat 327
- 327327
- Kehlung der Aufnahmen 326'Fillet of the recordings 326'
- 328328
- Probenbehälter, Probenröhrchen 50 mlSample container, sample tube 50 ml
- 330330
-
Einbuchtungen des Ergänzungsteils 320Indentations in the
supplementary part 320 - 334334
-
Bodenfläche des Ergänzungsteils 320Floor area of the
supplementary part 320 - 336336
-
ringförmige Vorsprünge des Ergänzungsteils 320annular projections of the
supplementary part 320 - 338338
- Probenträger, Mikrotiter-PlatteSample carrier, microtiter plate
- 340340
-
Grundfläche der Mikrotiter-Platte 338Base area of the
microtiter plate 338 - 342342
-
Rand der Grundfläche 340Edge of the
base area 340 - 400, 410, 420400, 410, 420
- Aufnahmen von ZentrifugeneinsätzenPhotographs of centrifuge inserts
- 402, 412, 422402, 412, 422
- ProbenbehälterSample container
- DD
- DrehachseAxis of rotation
Claims (10)
- Centrifuge insert (10) for receiving one or more elements from the group of sample containers (74, 76) and sample holders (78) in a centrifuge rotor (108), preferably configured as a free rotor, of a centrifuge (100), in particular a laboratory centrifuge, wherein the centrifuge insert (10) is configured to selectively receive at least one first element (74, 76), which has a circular or oval cross-section at least in some areas, and at least one second element (78), which has a polygonal cross-section at least in some areas, characterized in that the centrifuge insert (10) is configured in a modular manner with a centrifuge body (10) and a centrifuge rotor (108), and at least one second element (78), which has a polygonal cross-section at least in some areas, characterized in that the centrifuge insert (10) is of modular design with a base body (12) and at least one complementary part (16), wherein the complementary part (16) has an outer geometry which, together with a corresponding inner geometry of the base body (12), forms one or more second receptacles (72) for first elements (76).
- Centrifuge insert (10) according to claim 1, characterized in that the first element is a sample container, preferably a sample vial (74), a sample beaker, a test tube (76), a flexible tube, a reaction vessel, a centrifugation vessel, a vial, a microreaction vessel or a cell culture flask and in particular has a conically designed bottom part and/or in that the second element is a sample holder (78), preferably a plate-shaped element, in particular in the form of a microplate, microtitre plate, PCR plate or Deepwell plate, with a plurality of receptacles for sample.
- Centrifuge insert (10) according to claim 1 or 2, characterized in that the centrifuge insert (10) is configured to receive the elements (74, 76, 78) in a form-fitting manner and/or in that the configuration of the bottom of the receptacles of the centrifuge insert is adapted to the configuration of the bottom of the sample containers (74, 76) and/or sample holders (78) to be received, and preferably has a flat, conical or rounded contour.
- Centrifuge insert (10) according to one of the preceding claims, characterized in that the counterpart (16) is configured such that it can be adjusted, preferably plugged, into the basic body (12) and/or can be placed, preferably plugged, onto the basic body, in particular a plug-in connection, preferably in the form of a tongue-and-groove connection, can be formed between the basic body (12) and the counterpart (16).
- Centrifuge insert (10) according to one of the preceding claims, characterized in that the insert comprises one or more first receptacles for first elements.
- Centrifuge insert (10) according to one of the preceding claims, characterized in that the centrifuge insert (10) is configured for lateral attachment of first (74, 76) and/or second elements (78).
- Centrifuge insert (10) according to one of the preceding claims, characterized in that the centrifuge insert (10) comprises one or more flanges (28, 30, 34, 34', 34") or projections, which are adapted to laterally surround, at least in part, at least one of the first and second elements (78) and/or in that the centrifuge insert has one or more recesses (29, 31) or grooves (35, 35', 35"), which are adapted to laterally surround, at least partially, at least one of the first and second elements (78), wherein the centrifuge insert (10) is configured in particular to receive the first elements (74, 76) by plugging into corresponding receptacles (59, 72) of the centrifuge insert (10) and/or to carry the second elements (78), wherein the second elements (78) are locked laterally by positive fit preferably on a base surface (32).
- Centrifuge insert (10) according to one of the preceding claims, characterized in that the centrifuge insert (10) comprises at least two oppositely arranged guides (312), which are configured to laterally support at least one complementary part (320) and/or at least one second element, preferably at least two complementary parts or second elements stacked one on top of the other.
- Centrifuge insert (10) according to claim 8, characterized in that the guides have snap-fit connection elements, which are adapted to provide a snap-fit connection between the guides and the complementary part and/or the second element.
- Centrifuge insert (10) according to one of the preceding claims, characterized in that the centrifuge insert comprises handles (36) for gripping the centrifuge insert (10), which are configured in particular in the form of the guides according to one of claims 8 or 9.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102017125306.8A DE102017125306A1 (en) | 2017-10-27 | 2017-10-27 | centrifuge insert |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3476489A1 EP3476489A1 (en) | 2019-05-01 |
EP3476489B1 true EP3476489B1 (en) | 2023-09-20 |
Family
ID=63965263
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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EP18201796.2A Active EP3476489B1 (en) | 2017-10-27 | 2018-10-22 | Centrifuge insert |
Country Status (5)
Country | Link |
---|---|
US (1) | US11666927B2 (en) |
EP (1) | EP3476489B1 (en) |
JP (1) | JP6843102B2 (en) |
CN (1) | CN109718951B (en) |
DE (1) | DE102017125306A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US10625273B2 (en) * | 2012-09-03 | 2020-04-21 | Eppendorf Ag | Centrifuge insert and carrier for centrifuge insert with snap locking connection |
CN112517103A (en) * | 2020-12-24 | 2021-03-19 | 苏州市立医院 | Controllable formula centrifuge tube rack |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20160250608A1 (en) * | 2015-02-27 | 2016-09-01 | Heathrow Scientific Llc | Head for a mixing apparatus |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5610946U (en) | 1979-07-04 | 1981-01-30 | ||
DD149615A1 (en) * | 1980-03-26 | 1981-07-22 | Roland Lippold | SUBSTANCE FOR LABORATORY CENTRIFUGE FOR THE RECORDING OF SAMPLE WEBSITES |
JPS59171759A (en) | 1983-03-16 | 1984-09-28 | Hitachi Ltd | Electrically-driven power steering apparatus |
US4531652A (en) | 1984-06-25 | 1985-07-30 | Kabushiki Kaisha Kubota Seisakusho | Bucket for use in centrifugal separators |
DE3425922C2 (en) * | 1984-07-13 | 1986-05-28 | Kabushiki Kaisha Kubota Seisakusho, Tokio/Tokyo | Bucket for hanging on a swivel rotor of a centrifuge |
US5549540A (en) * | 1994-10-13 | 1996-08-27 | Baxter International Inc. | Centrifuge insert for support of fluid containing bags |
DE102012013642B4 (en) * | 2012-07-09 | 2020-12-24 | Thermo Electron Led Gmbh | Centrifuge tube unit |
US10625273B2 (en) * | 2012-09-03 | 2020-04-21 | Eppendorf Ag | Centrifuge insert and carrier for centrifuge insert with snap locking connection |
CN202778745U (en) * | 2012-09-28 | 2013-03-13 | 黑龙江八一农垦大学 | Set combined type centrifugal-machine adapter |
US9180461B2 (en) * | 2012-10-22 | 2015-11-10 | Qiagen Gaithersburg, Inc. | Condensation-reducing incubation cover |
DE202013006223U1 (en) * | 2013-07-10 | 2014-10-13 | Eppendorf Ag | Sampler insert for holding multiple sample tubes in a laboratory container and laboratory container with this sample holder insert |
DE112015004494B4 (en) * | 2014-09-30 | 2021-03-04 | Eppendorf Himac Technologies Co., Ltd. | Centrifuge and swing-bucket rotor for a centrifuge |
US20170368560A1 (en) * | 2016-06-27 | 2017-12-28 | Flotek Chemistry, Llc | Centrifuge container adapter and related articles, systems, and methods |
JP2018201439A (en) | 2017-06-07 | 2018-12-27 | 則雄 安井 | Roasting device |
-
2017
- 2017-10-27 DE DE102017125306.8A patent/DE102017125306A1/en active Pending
-
2018
- 2018-10-22 EP EP18201796.2A patent/EP3476489B1/en active Active
- 2018-10-26 JP JP2018201439A patent/JP6843102B2/en active Active
- 2018-10-27 US US16/172,752 patent/US11666927B2/en active Active
- 2018-10-29 CN CN201811266338.6A patent/CN109718951B/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20160250608A1 (en) * | 2015-02-27 | 2016-09-01 | Heathrow Scientific Llc | Head for a mixing apparatus |
Also Published As
Publication number | Publication date |
---|---|
JP2019081169A (en) | 2019-05-30 |
CN109718951A (en) | 2019-05-07 |
US20190151862A1 (en) | 2019-05-23 |
DE102017125306A1 (en) | 2019-05-02 |
US11666927B2 (en) | 2023-06-06 |
JP6843102B2 (en) | 2021-03-17 |
EP3476489A1 (en) | 2019-05-01 |
CN109718951B (en) | 2022-06-03 |
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