EP3485977B1 - Centrifugeuse et rotor libre - Google Patents
Centrifugeuse et rotor libre Download PDFInfo
- Publication number
- EP3485977B1 EP3485977B1 EP18206065.7A EP18206065A EP3485977B1 EP 3485977 B1 EP3485977 B1 EP 3485977B1 EP 18206065 A EP18206065 A EP 18206065A EP 3485977 B1 EP3485977 B1 EP 3485977B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- rotor
- swing
- centrifuge
- recesses
- axis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000008878 coupling Effects 0.000 claims description 7
- 238000010168 coupling process Methods 0.000 claims description 7
- 238000005859 coupling reaction Methods 0.000 claims description 7
- 238000011161 development Methods 0.000 description 9
- 230000018109 developmental process Effects 0.000 description 9
- 238000009434 installation Methods 0.000 description 5
- 239000000969 carrier Substances 0.000 description 3
- 238000001816 cooling Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
- B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
- B04B11/04—Periodical feeding or discharging; Control arrangements therefor
- B04B2011/046—Loading, unloading, manipulating sample containers
Definitions
- the present invention relates to a centrifuge according to the preamble of claim 1 and a swing-bucket rotor according to the preamble of claim 8.
- Centrifuge rotors are used in centrifuges, in particular laboratory centrifuges, in order to separate the components of samples centrifuged therein using mass inertia. Increasingly higher rotation speeds are used to achieve higher segregation rates.
- Laboratory centrifuges are centrifuges whose rotors work at preferably at least 3,000, preferably at least 10,000, in particular at least 15,000 revolutions per minute and are mostly placed on tables. In order to be able to place them on a work table, they have in particular a form factor of less than 1 m ⁇ 1 m ⁇ 1 m, so their installation space is limited.
- the device depth is preferably limited to a maximum of 70 cm.
- centrifuges are used in the fields of medicine, pharmacy, biology and chemistry and the like.
- samples are centrifuged at certain temperatures.
- samples that contain proteins and similar organic substances must not be overheated, so that the upper temperature limit for such samples is usually around +40°C.
- certain samples are cooled by default in the +4°C range (water anomaly starts at 3.98°C).
- Active and passive systems can be used for temperature control.
- Active cooling systems have a refrigerant circuit that covers the centrifuge container tempered, which indirectly cools the centrifuge rotor and the sample containers contained therein.
- Passive systems are based on exhaust air-supported cooling or ventilation. This air is routed directly past the centrifuge rotor, which results in temperature control. The air is sucked into the centrifuge chamber through openings, whereby the suction takes place automatically through the rotation of the centrifuge rotor.
- the samples to be centrifuged are stored in sample containers and these sample containers are driven in rotation by means of a centrifuge rotor.
- the centrifuge rotors are usually set in rotation by means of a vertical drive shaft that is driven by an electric motor. There are different centrifuge rotors that are used depending on the application.
- the sample containers can contain the samples directly, or separate sample containers containing the sample are inserted into the sample containers, so that a large number of samples can be centrifuged simultaneously in one sample container.
- centrifuge rotors are known in the form of fixed-angle rotors and swing-bucket rotors.
- Such fixed-angle rotors usually have a lower part and a cover, with the closed state of the cover forming an interior space between the lower part and the cover, in which the sample vessels can be arranged in order to centrifuge the samples in a suitable centrifuge.
- a swing-out rotor is present if there is at least one hanger in the centrifuge rotor which can swing out from a vertical position to a horizontal position depending on the rotational speed of the centrifuge rotor.
- the hanger is mounted on a rotor body so that it can swing out.
- Such hangers are usually designed as centrifuge buckets.
- hangers are also known in which the sample container itself is provided with appropriate coupling means for coupling to the centrifuge rotor and is thus held in the centrifuge rotor so that it can swing out.
- sample is therefore understood not only as a holder for sample containers, but also as sample containers that are mounted such that they can swing out.
- the present invention is based on such swing-bucket rotors.
- the swing-bucket rotor For connection to the centrifuge, the swing-bucket rotor is usually provided with a hub which can be coupled to the motor-driven drive shaft of the centrifuge.
- the hangers of the swing-bucket rotor usually have a lower part that is closed by a cover.
- the lid of the hanger is designed so that it can normally be closed with the lower part.
- hangers are also known which do not have a cover.
- aerosol-tight seal between the cover and the base.
- the aerosol-tight seal allows the carriers to be easily transported and manipulated without the risk of the samples contaminating the centrifuge or the environment.
- the closure between the lid and the base can be designed in different ways.
- swing-bucket rotors take up a relatively large amount of space due to the pivoting of the hanger that occurs during swing-out, so that the centrifuges used for centrifuging must be dimensioned relatively large compared to fixed-angle rotors.
- the U.S. 3,028,075 A describes a centrifuge with a swing-bucket rotor having six trunnion rings. These trunnion rings are mounted on bolts, allowing them to oscillate in a radial plane. Within the trunnion ring there are sample holders, which are mounted horizontally pivotable relative to the trunnion ring via bolts, the pivotability taking place here in a tangential plane. There are also recesses for the bearing journal rings.
- the DE 10 2006 027 680 A1 shows a swing-out rotor with a swing-out container, which has stops for maximum deflection positions opposite the arms of the centrifuge rotor.
- the swing-out container has a horizontal stop edge and a inclined running stop edge on and on the rotor arms are corresponding stop edges.
- the U.S. 3,377,021 A shows a swing-bucket rotor that has receptacles for sample containers to be suspended. These sample containers have a receiving area for samples that can be closed with a lid.
- the U.S. 4,435,168 A shows a centrifuge rotor with swing-out containers, which are rotatably mounted between rotor arms about pivots.
- the rotor arms have a continuous slot that ends in a recess. Bearing pieces are inserted into this recess, with the two webs of the rotor arm being able to be bent up slightly by means of the slot in order to be able to insert and clamp the bearing pieces.
- the object of the present invention to design the swing-bucket rotor in such a way that this disadvantage is reduced.
- the swing-bucket rotor should require less installation space with the same load capacity (ie sample quantity to be taken up).
- the centrifuge provided with the swing-bucket rotor should preferably be able to have smaller dimensions or, with the same dimensions, be able to take up a larger sample quantity.
- the inventors recognized that this task can be solved in a surprising manner in a particularly simple manner if recesses are provided in the swing-bucket rotor, into which at least parts of the hanger and/or sample containers mounted therein can dip when the hanger swings out.
- the swing-bucket rotor can be designed much more compactly with the same loading capacity of the hanger.
- areas of the cover, areas of the lower part or areas of both the lower part and the cover dip into the recesses when swinging out.
- the centrifuge according to the invention which is in particular a laboratory centrifuge, comprises a swing-bucket rotor driven by drive means, which has one or more hangers, preferably buckets, and a rotor hub for coupling to the drive means, with the hanger being mounted so that it can swing out between two rotor arms of a rotor base body, and is characterized characterized in that the rotor arms have recesses into which the hanger and/or the sample containers mounted therein dip at least partially in the swung-out state.
- the recesses are formed as grooves, in particular as fluting. They can then be introduced into the swing-bucket rotor in a particularly simple manner, for example as part of a forming process when producing the basic rotor body.
- the recesses are rounded. As a result, only the smallest possible recess is required for immersing the hanger.
- the recesses extend over a length of the rotor arm that is shorter than the distance between the rotor hub and the mounting of the hangers. Then the stability of the swing-bucket rotor is guaranteed even in the limit range.
- the recesses extend over a height of the rotor arm which is less than the height of the rotor arm at the location of the recess, the recesses being delimited in particular by upper and lower webs. Then the stability of the swing-bucket rotor is guaranteed even in the limit range.
- two recesses arranged on different sides of a rotor arm are connected by an opening.
- the connected cavities may have the cross-sectional shape of a diabolos with a central passage.
- the rotor arms are designed with a smaller cross-section at the location of the recess than in areas without a recess. This reduces the inertial mass of the swing-bucket rotor, which reduces the energy requirement when starting centrifugation.
- the recesses cover a region of the rotor arms which, in relation to the radius of the swing-out rotor, corresponds to half the distance between the axis of rotation of the swing-out rotor and the bearing of the hanger. Then the swing-bucket rotor is particularly compact.
- the swing-bucket rotor has at least three, preferably at least four, rotor arms for at least three, preferably at least four hangers mounted between them.
- rotor arms for at least three, preferably at least four hangers mounted between them.
- five or more rotor arms can also be provided in order to store an equal number of hangers in between so that they can swing out.
- the swing-bucket rotor according to the invention for a centrifuge, which can be driven by a drive means of the centrifuge, the swing-bucket rotor having one or more hangers, preferably cups, and a rotor hub for coupling to the drive means, the hanger between two rotor arms of a rotor base body is mounted such that it can swing out, and is characterized in that the rotor arms have recesses into which the hanger and/or the sample container mounted therein at least partially immerse in the swing-out state.
- the swing-bucket rotor has at least one of the features of the centrifuge according to the invention.
- the centrifuge 10 is designed as a laboratory centrifuge which has a housing 14 with a cover 16 .
- the swing-bucket rotor 12 is arranged in the centrifuge container 18 of the centrifuge 10 on a drive shaft (not shown) of a centrifuge motor (not shown).
- the swing-bucket rotor 12 has a basic rotor body 20 with a hub 22 which is coupled to the drive shaft via screws 23 .
- the basic rotor body 20 has four rotor arms 24, which are each offset from one another in the circumferential direction by 90° on the hub 22.
- the rotor arms 24 open into arm branches 26, on each of which two retaining pins 28 are arranged, the oppositely arranged retaining pins 28, 28' of two oppositely arranged arm branches 26, 26' being aligned in such a way that they lie in one line and thus form a swing-out axis form A.
- the hangers 30 are designed as cups 32 with lids 34 .
- samples that are included in sample containers or sample carriers and where the sample containers or sample carriers are optionally arranged in adapters (all not shown), are arranged in order to centrifuge them using the centrifuge 10 .
- the hangers 30 have pin receptacles 36 which have an upper stop 38 and two lateral guides 40 .
- the hangers 30 can be arranged on the retaining pins 28 with the aid of these pin receptacles 36 .
- the upper stops 38 rest on the retaining pins 28 and the lateral guides 40, together with a design of the pin receptacles 36 that fits in relation to the retaining pins 28, form a pivot bearing 41 about the swing-out axis A, so that the hanger 30 about the swing-out axis A from the in 2 non-swing position shown in the in 3 swing out position shown.
- the non-swinged-out position in which the longitudinal axis L of the hanger extends parallel to the axis of rotation D of the swing-out rotor 12, is assumed by the hanger 30 while the swing-out rotor 12 is at a standstill.
- the swing-out position in which the longitudinal axis L of the hanger extends perpendicularly to the axis of rotation D of the swing-bucket rotor 12, is assumed by the hanger 30 while the swing-bucket rotor 12 is operating at a sufficient speed.
- the rotor arms 24 have a slightly conically tapering cross section, the thickness B being constant over the entire height H, except for the areas in which the recesses 42 are provided. There, the thickness B' is significantly reduced, as is particularly evident in figure 5 can be seen. These recesses 42 extend over an area that lies halfway between the swing-out axis A and the axis of rotation D in relation to the radius of the swing-out rotor 12 .
- the recesses 42 are fluted and rounded and the lids 34 of the cups 32 can dip into them when swung out, as in FIG 3 can be seen.
- the swing-out axes A can be brought closer to the axis of rotation D with an identical hanger 30 .
- the centrifuge containers 18 and thus also the centrifuges 10 can be made much more compact because swinging out now requires less installation space.
- both the centrifuge container 18 and the basic rotor body 20 can be retained and instead the hangers 30 can be enlarged so that a larger sample quantity can be centrifuged.
- the swing-bucket rotor 12 can also be designed in such a way that only the cup 32 itself or the cup 32 and cover 34 together can enter the recess.
- the hanger 52 itself does not have to dip into the recess 42, but it can also be provided that a sample container 54 arranged in the hanger 52, in which a sample 56 is located , Immersed in this recess 42 during swing, as 6 and according to the relevant detailed view 7 can be removed. The installation space is then also made more compact.
- the present invention provides a centrifuge 10 and a swing-bucket rotor 12, 50 with which the installation space within the centrifuge 10 can be used much better.
Landscapes
- Centrifugal Separators (AREA)
Claims (9)
- Centrifugeuse (10), en particulier centrifugeuse de laboratoire, comportant un rotor libre (12 ; 50) entraîné par un moyen d'entraînement, ledit rotor libre possédant un axe de rotation (D) et présentant un ou plusieurs éléments suspendus (30 ; 52), de préférence des godets (32), qui présentent un axe longitudinal d'élément suspendu (L), et un moyeu de rotor (22) destiné à l'accouplement (23) avec le moyen d'entraînement, l'élément suspendu (30 ; 52) étant monté de manière à pouvoir basculer entre deux bras de rotor (24) d'un corps de base de rotor (20), un état basculé étant présent lorsque l'axe longitudinal d'élément suspendu (L) s'étend perpendiculairement à l'axe de rotation (D) du rotor libre (12), les bras de rotor (24) présentant des évidements (42), dans lesquels l'élément suspendu (30) et/ou des récipients d'échantillon (54) logés dans celui-ci s'enfoncent au moins par endroits dans l'état basculé, caractérisée en ce que les évidements (42) recouvrent une partie des bras de rotor (24) qui correspond, par rapport au rayon du rotor libre (12), à la moitié de la distance entre l'axe de rotation (D) du rotor libre (12) et le logement (41) de l'élément suspendu (30 ; 52).
- Centrifugeuse (10) selon la revendication 1, caractérisée en ce que les évidements (42) sont réalisés sous la forme de gorges, en particulier sous la forme de cannelures et/ou en ce que les évidements (42) sont réalisés arrondis.
- Centrifugeuse (10) selon la revendication 1 ou 2, caractérisée en ce que les évidements (42) s'étendent sur une longueur du bras de rotor (24) qui est inférieure à la distance entre le moyeu de rotor (22) et le logement (41) des éléments suspendus (30).
- Centrifugeuse (10) selon l'une des revendications précédentes, caractérisée en ce que les évidements (42) s'étendent sur une hauteur du bras de rotor (24) qui est inférieure à la hauteur (H) du bras de rotor (24) à l'endroit de l'évidement (42), les évidements (42) étant en particulier limités par des rebords (44) supérieurs et inférieurs.
- Centrifugeuse selon l'une des revendications précédentes, caractérisée en ce que deux évidements disposés sur des côtés différents d'un bras de rotor sont reliés par une ouverture.
- Centrifugeuse (10) selon l'une des revendications précédentes, caractérisée en ce que le bras de rotor (24), à l'endroit de l'évidement (42), est réalisé avec une section transversale inférieure à celle de zones sans évidement (42).
- Centrifugeuse (10) selon l'une des revendications précédentes, caractérisée en ce que le rotor libre (12) présente au moins trois, de préférence au moins quatre, bras de rotor (24) pour au moins trois, de préférence au moins quatre, éléments suspendus (30) logés entre ceux-ci.
- Rotor libre (12 ; 50) pour une centrifugeuse (10), qui peut être entraînée par un moyen d'entraînement de la centrifugeuse (10), le rotor libre (12 ; 50) possédant un axe de rotation (D) et présentant un ou plusieurs éléments suspendus (30 ; 52), de préférence des godets (32), et un moyeu de rotor (22) destiné à l'accouplement (23) avec le moyen d'entraînement, l'élément suspendu (30 ; 52) étant monté de manière à pouvoir basculer entre deux bras de rotor (24) d'un corps de base de rotor (20), un état basculé étant présent lorsque l'axe longitudinal d'élément suspendu (L) s'étend perpendiculairement à l'axe de rotation (D) du rotor libre (12), les bras de rotor (24) présentant des évidements (42), dans lesquels l'élément suspendu (30) et/ou des récipients d'échantillon (54) logés dans celui-ci s'enfoncent, au moins par endroits, dans l'état basculé, caractérisé en ce que les évidements (42) recouvrent une partie des bras de rotor (24) qui correspond, par rapport au rayon du rotor libre (12), à la moitié de la distance entre l'axe de rotation (D) du rotor libre (12) et le logement (41) de l'élément suspendu (30 ; 52).
- Rotor libre (12 ; 52) selon la revendication 8, caractérisé par les caractéristiques concernant le rotor libre d'au moins une des revendications 2 à 7.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102017127039.6A DE102017127039A1 (de) | 2017-11-16 | 2017-11-16 | Zentrifuge und Ausschwingrotor |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3485977A1 EP3485977A1 (fr) | 2019-05-22 |
EP3485977B1 true EP3485977B1 (fr) | 2023-08-23 |
Family
ID=64316301
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP18206065.7A Active EP3485977B1 (fr) | 2017-11-16 | 2018-11-13 | Centrifugeuse et rotor libre |
Country Status (5)
Country | Link |
---|---|
US (1) | US11247215B2 (fr) |
EP (1) | EP3485977B1 (fr) |
JP (1) | JP7161921B2 (fr) |
CN (1) | CN109794365A (fr) |
DE (1) | DE102017127039A1 (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113145319B (zh) * | 2021-04-30 | 2022-03-08 | 江苏汉典生物科技股份有限公司 | 一种益生菌粉的制备工艺 |
CN113578537A (zh) * | 2021-09-09 | 2021-11-02 | 北京金诺美生物技术有限公司 | 一种离心角度自适应的离心机 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3028075A (en) * | 1959-01-12 | 1962-04-03 | Sorvall Inc Ivan | Swinging bucket centrifuge |
US3377021A (en) * | 1965-05-17 | 1968-04-09 | Internat Equipment Company | Centrifuge rotors, buckets and combinations of such buckets and rotors |
US3393864A (en) * | 1966-04-11 | 1968-07-23 | Beckman Instruments Inc | Centrifuge apparatus |
US3687359A (en) | 1971-01-07 | 1972-08-29 | Damon Corp | Centrifuge rotor |
JPS4833059A (fr) | 1971-05-19 | 1973-05-07 | ||
US4009824A (en) * | 1975-12-31 | 1977-03-01 | Beckman Instruments, Inc. | Swinging bucket centrifuge rotor |
JPS5632029A (en) | 1979-08-23 | 1981-04-01 | Nissan Motor Co Ltd | Cooling system for automobile internal-combustion engine |
US4344563A (en) | 1980-12-23 | 1982-08-17 | E. I. Du Pont De Nemours And Company | Centrifuge rotor having vertically offset trunnion pins |
US4400166A (en) | 1981-12-28 | 1983-08-23 | Beckman Instruments, Inc. | Top loading centrifuge rotor |
US4435168A (en) * | 1982-06-04 | 1984-03-06 | Damon Corporation | Centrifuge rotor apparatus with sling arms |
JP4713764B2 (ja) | 2001-05-18 | 2011-06-29 | オリンパス株式会社 | データ再生装置 |
TWI372050B (en) | 2003-07-03 | 2012-09-11 | Astex Therapeutics Ltd | (morpholin-4-ylmethyl-1h-benzimidazol-2-yl)-1h-pyrazoles |
DE102006027680A1 (de) * | 2006-06-14 | 2007-12-20 | Qiagen Gmbh | Apparat zum Prozessieren von biologischem Material |
EP2295982B1 (fr) | 2005-09-26 | 2018-09-05 | QIAGEN GmbH | Dispositif pour le traitement des matériau biologique |
JP5707882B2 (ja) * | 2010-11-12 | 2015-04-30 | 日立工機株式会社 | 遠心分離機用スイングロータ及び遠心分離機 |
JP6435778B2 (ja) | 2014-10-30 | 2018-12-12 | 工機ホールディングス株式会社 | 遠心機用スイングロータ及び遠心機 |
JP6406033B2 (ja) * | 2015-01-28 | 2018-10-17 | 工機ホールディングス株式会社 | 遠心機及び遠心機用スイングロータ |
-
2017
- 2017-11-16 DE DE102017127039.6A patent/DE102017127039A1/de active Pending
-
2018
- 2018-11-13 EP EP18206065.7A patent/EP3485977B1/fr active Active
- 2018-11-15 JP JP2018214700A patent/JP7161921B2/ja active Active
- 2018-11-15 US US16/191,942 patent/US11247215B2/en active Active
- 2018-11-16 CN CN201811366960.4A patent/CN109794365A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
US11247215B2 (en) | 2022-02-15 |
CN109794365A (zh) | 2019-05-24 |
JP7161921B2 (ja) | 2022-10-27 |
EP3485977A1 (fr) | 2019-05-22 |
DE102017127039A1 (de) | 2019-05-16 |
US20190143341A1 (en) | 2019-05-16 |
JP2019089061A (ja) | 2019-06-13 |
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