EP2885054A1 - Préparation à l'aide de plantes pour la stimulation de la croissance capillaire, la lutte contre la chute des cheveux, les pellicules et les infections de ceux-ci à l'aide d'ageratum spp - Google Patents

Préparation à l'aide de plantes pour la stimulation de la croissance capillaire, la lutte contre la chute des cheveux, les pellicules et les infections de ceux-ci à l'aide d'ageratum spp

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Publication number
EP2885054A1
EP2885054A1 EP13879457.3A EP13879457A EP2885054A1 EP 2885054 A1 EP2885054 A1 EP 2885054A1 EP 13879457 A EP13879457 A EP 13879457A EP 2885054 A1 EP2885054 A1 EP 2885054A1
Authority
EP
European Patent Office
Prior art keywords
hair
herbal
acid
herbal extract
ageratum spp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP13879457.3A
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German (de)
English (en)
Inventor
Vaiyapuri Ramalingam PRABAVATHY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gencor Armats Biotek Pvt Ltd
Original Assignee
Gencor Armats Biotek Pvt Ltd
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Filing date
Publication date
Application filed by Gencor Armats Biotek Pvt Ltd filed Critical Gencor Armats Biotek Pvt Ltd
Publication of EP2885054A1 publication Critical patent/EP2885054A1/fr
Withdrawn legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/31—Hydrocarbons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/342—Alcohols having more than seven atoms in an unbroken chain
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/35—Ketones, e.g. benzophenone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/368—Carboxylic acids; Salts or anhydrides thereof with carboxyl groups directly bound to carbon atoms of aromatic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/37—Esters of carboxylic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/63—Steroids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/69—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing fluorine
    • A61K8/70—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing fluorine containing perfluoro groups, e.g. perfluoroethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/006—Antidandruff preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth

Definitions

  • Embodiments are generally related to the field of pharmaceutics, cosmetics and allied industries. Embodiments are also related to herbal extracts for stimulation of hair growth, control of hair fall, dandruff and infections thereof. Embodiments are particularly related to an herbal extraction process for preparing hair growth simulator using herbaceous plants, Ageratum spp. (Ageratum conyzoides (Synonym: Ageratum cperuleum) and Ageratum houstonianum)
  • Hair loss/hair fall has become a ubiquitous affliction of human beings (both male and female gender) with the growing impact of global pollution and lifestyle changes.
  • Most common reasons of radical or premature hair loss/hair fall are genetic predisposition, endocrine disorders, medication, radiation, chemotherapy, and exposure to chemicals, nutritional factors, generalized or local skin diseases, stress, child birth, Alopecia areata and mechanical damage such as Trichotillomania, hair styling treatment, hair braids and weaves.
  • the hair in humans is generated by the hair follicles implanted in the scalp.
  • a healthy head of hair is said to contain between 100000 and 150000 hairs, and each hair within this head of hair possesses its own cycle.
  • the life cycle of the hair can be. described in three successive physiological phases such as, Anagen, Catagen and Telogen.
  • Anagen is a phase of hair growth which may last from a few weeks to 10 years
  • Catagen phase is a transient phase of involution of the follicle and ceasing of hair growth with degeneration of the root which may last for a few weeks
  • Telogen is a phase of shedding of the hair with the root moving up towards the surface by lasting 1 to 5 months.
  • Telogen phase the hair therefore disappears from the scalp and this disappearance may extend from a few days to a few months before the follicle is reactivated to give a new hair in the Anagen phase.
  • plugs of skin containing hair are transplanted from areas of the scalp where hair was growing to bald or balding areas of the scalp.
  • the transplantation technique can be a time-consuming and relatively costly affair.
  • Other approaches include ultra-violet radiation and exercise therapy. While cosmetic aids are another significant approach for the stimulation of hair-growth, there is still a need in the art for compounds which may have greater stimulatory effect on hair growth.
  • An herbal preparation for stimulation of hair growth and control of hair fall, dandruff and infections thereof using herbaceous plants, Ageratum spp. (including Ageratum conyzoides, Ageratum coeru!eum and Ageratum houstonianum) is disclosed herein. At least one compound of plants, Ageratum spp.
  • CPEA Common Plant Extraction Approach
  • the extraction of compounds can be performed utilizing an ethanol solvent and a methanol solvent (i.e., alcoholic solvents) separately and the concentrated materials obtained at both the solvents are pooled together (or separately) in order to obtain an herbal extract for simulating the follicles of the hair.
  • the compounds of plants, Ageratum spp. in combination with an acceptable carrier or diluent can be further prepared into an herbal extract formulations such as, a liquid, oil or gel formulation for topical application.
  • Such an herbal preparation using plants, Ageratum spp. can be utilized as an efficient hair care solution in a wide range of hair growth related problems of humans in a cost effective manner.
  • the Common Plant Extraction Approach (CPEA) described herein can be a principle extraction technique that is well known in the art for extracting the compounds of a plant. Initially, the leaves and soft stems of Ageratum spp. (preferably 60 to 90 days old plants) can be sliced into pieces (approx. 1 cm size) and shade dried at room temperature. The sliced pieces can be also dried using a fluid flash dryer. Further, a mechanized pulverizing machine can be employed to grind the dried plant slices into coarse powder in order to thereby extract the compounds using the ethanol and methanol solvents separately.
  • the solvent extraction process described herein can be performed at a 1 :10 (W/V) ratio using a soxhlet apparatus in order to thereby separate the green colored solvent extracts using a rotoevaporator.
  • the compounds obtained at both the ethanol solvent extraction process and the methanol solvent extraction process are further pooled together (or used separately) in order to form the herbal extract for simulating the hair growth.
  • the compounds extracted at the methanol solvent extraction process can be such as, for example, but not limited to, N ) N-bis(trimethylsilyl)-2-phenyl-7-(trifluoromethyl)quinolon-4-amine, 1 - deoxy-d-mannitol, 15-hydroxypentadecanoic acid, 2-chromenone, 3',4 ⁇ 5, 6,7,8- hexamethoxyflavone, 3,5-di-tert-butylbenzoic acid, 3,7, 1 ,15-tetramethylhexadec-2-eh- 1-ol, 4-tert-butyl-2,6-dimethylacetophenone, 6-vinyl-7-methoxy-2,2-dimethylchromene, Anhydro-d-mannosan, Beta-Funebre
  • the compounds extracted at the ethanol solvent extraction process can be such as, for example, but not limited to, N,N-bis(trimethylsilyl)-2-phenyl-7- (trifluoromethyl)quinolon-4-amine, (Z)-,Beta-farnesene, 1-(3,5-ditert-butyl-4- hydroxyphenyl)ethanone, 2-(1 ,3-benzodioxol-5-yl)-5-hydroxy-3,6,7,8-tetramethoxy-4h- chromen-4-one, 2-(3,4-dimethoxyphenyl)-5,6,7,8-tetramethoxy-4H-1 -benzopyran-4-one, 2,4,6-tri-tert-butylphenol, 2-chromanone, 2H-chromen-2-one, 3', 4', 5,6,7, 8- hexamethoxyflavone, 3,5-di-tert-butylbenzoic acid, 3,7, 11
  • the solvents utilized for extracting compounds herein should not be construed in any limited sense. Those skilled in the art can understand that similar kind of solvents such as hexane, ethyl acetate, acetone, chloroform, dichloromethane, etc. can also tried for preparing herbal extraction and developing the hair growth stimulant formulation.
  • the herbal extract is further formulated with an acceptable carrier or diluent in order to formulate as liquid, shampoo, oil or gel for topical application.
  • the .'nerbai extract can be dissolved in the sterile Distilled water (at 1 -5 g/l) and the aqueous suspension can be filtered (through 1 to 5 ⁇ filter, pH adjusted to neutral) in order to form a liquid formulation of the herbal extract.
  • the herbal extract is dissolved in vegetable oil or PG/PL and the suspension is filtered (through 1 to 5 ⁇ filter) in order to form an oil formulatidn.
  • the herbal extract can be dissolved in a small quantity of ethanol and filtered (through 1 to 5 ⁇ filter) in order to form the gel formulation.
  • the mixture can be mixed vigorously using mechanized homogenizer to get the uniform gel product.
  • FIG. 1 illustrates a perspective view of a process for preparing an herbal extract formulation using herbaceous plants, Ageratum spp., in accordance with the disclosed embodiments.
  • FIG. 2 illustrates a high level flow chart of operations illustrating logical operational steps of a method for preparing herbal extract formulation using herbaceous plants, Ageratum spp., in accordance with the disclosed embodiments.
  • FIG. 1 illustrates a perspective view of a process 100 for preparing an herbal extract formulation 190 using herbaceous plants, Ageratum spp. (including Ageratum conyzoides, Synonym: Ageratum coeruleum) and Ageratum houstonianum) .1 10, in accordance with the disclosed embodiments.
  • Ageratum spp. are annual herbaceous plants belonging to the family Asteraceae. They are common weeds and grow rapidly. They are known by several names such as goat weed, chick weed, white weed, etc.
  • Ageratum spp. are found in many countries ranging from Southeastern North America to Central America, Asia and Africa; but the center of origin is in Central America and the Caribbean. Ageratum spp. are also found in several countries in tropical and subtropical regions, including India, Brazil, Vietnam, Nigeria, Kenya, Congo, Cameroon, etc. in abundance.
  • Ageratum spp. are very valuable herbs as they contain many bioactive compounds including flavonoids, alkaloids, cumarins, essential oils, chromenes, benzofurans, terpenoids and tannins that have medicinal, antimicrobial and insecticidal properties.
  • the extracts of Ageratum conyzoides 1 10 and other Ageratum species have been used to cure a wide range of ailments such as muscle pains, inflammations, dysentery, indigestion, constipation, wounds in humans.
  • the use of Ageratum spp. 1 10 in stimulation of hair follicles is novel. It has not hitherto been known that Ageratum spp. 10 have a simulative action on hair growth and hair fall control.
  • a Common Plant Extraction Approach is adapted to prepare the herbal extract from Aageratum spp. 10.
  • the Common Plant Extraction Approach (CPEA) described herein can be a principle extraction technique that is well known in the art for extracting the compounds 180 of plants. Note that other extraction processes can be alternatively employed in the place of suggested without losing the scope of invention.
  • the leaves and soft stems of Ageratum spp. 0 preferably 60 to 90 days old plants are initially sliced into pieces 120 (i.e., approx. 1 cm) and shade dried at room temperature (approx. 28-30°C) for 48 hrs to 72 hrs.
  • The. sliced pieces 120 can be also dried at 45°C using a fluid flash dryer for 24 hrs.
  • the dried plant slices 120 are ground to course powder using a mechanized pulverizing machine 130 in order to thereby extract the compounds 180 using the ethanol solvent 160, methanol solvent 170 and other solvents separately.
  • the solvent extraction process described herein can be " performed at a 1 :10 (W/V) ratio using a soxhlet apparatus in order thereby separate the green colored solvent extracts using a rotoevaporator.
  • the solvents utilized for extracting compounds herein should not be construed in any limited sense. Those skilled in the art can understand that similar kind of solvents such as hexane, ethy! acetate, acetone, chloroform, dichloromethane, etc. can also tried for preparing herbal extraction and developing the hair growth stimulant formulation.
  • the compounds extracted at the methanol solvent extraction process 150 can be such as, for example, but not limited to, N,N-bis(trimethylsilyl)-2-phenyl-7- (trifluoromethyl)quinolon-4-amine, -deoxy-d-mannitol, 5-hydroxypentadecanoic acid, 2-chromenone, 3',4',5,6,7,8-hexamethoxyflavone, 3,5-di-tert-butylbenzoic acid, 3,7,1 1 ,15-tetramethylhexadec-2-en-1-ol, 4-tert-butyl-2,6-dimethylacetophenone, 6-vinyl- 7-methoxy-2,2-dimethylchrpmene, Anhydro-d-mannosan, Beta-Funebrene, Caryophyllene, Coumarin, Hexa-o-methylmyricitin, Hydrocoumarin, LinoleoyI chloride, Methyl c
  • Table 1 illustrates the list of compounds extracted by treating the plant material 120 using the methanol solvent 170.
  • the compounds extracted at the ethanol solvent extraction process can be such as, for example, but not limited to, N,N-bis(trimethylsilyl)-2-phenyl- 7-(trifluoromethyl)quinolon-4-amine, (Z)-,Beta-famesene, 1 -(3,5-ditert-butyl-4- hydroxyphenyl)ethanone, 2-(1 ,3-benzodioxol-5-yl)-5-hydroxy-3,6,7,8-tetramethoxy-4h- chromen-4-one, 2-(3,4-dimethoxyphenyl)-5,6,7,8-tetramethoxy ⁇ 4H-1-benzopyran-4- one, 2,4,6-tri-tert-butylphenol, 2-chromanone, 2H-chromen-2-one, 3', 4', 5,6, 7,8- hexamethoxyflavone, 3,5-di-tert-butylbenzoic acid
  • Table 2 illustrates the list of compounds extracted by treating the plant material 120 using the ethanol solvent 170.
  • the green colored solvent extract from ethanol/methanol/other solvents are removed using vacuum evaporation.
  • Ageratum spp. four compounds, 9,12-octadecadienoic acid (Z,Z)-, Alpha-linolenic acid, Hexadeconoic acid and Hydrocoumarin are identified as 5 Alpha-reductase inhibitors.
  • the 5 alpha reductase enzyme metabolizes the testosterone (male hormone) into dihydrotestosterone (DHT), which is known to induce scalp hair loss in man.
  • DHT dihydrotestosterone
  • the 5 alpha reductase inhibitors present in the extracts of Ageratum spp. can attach with 5 alpha reductase enzyme and prevent it to convert the testosterone into DHT. Thereby decrease and stop the hair loss.
  • Table 3 illustrates the list of four compounds identified as 5 Alpha reductase inhibitors present in both ethanol and methanol extracts of the hair growth product.
  • Table 3 [0029] The compounds obtained at both the ethanol solvent extraction process 140 and the methanol solvent extraction process 150 (and other solvent processes) are further pooled together in order to form the herbal extract 180 for simulating the hair growth.
  • the green colored solvent extracts are separated and the ethanol/methanol/other solvents are removed using vacuum evaporation.
  • Table 4 illustrates the list of six major compounds identified as common components present in both ethanol and methanol extracts of the hair growth product.
  • the herbal extract contains the above 5 alpha reductase inhibitors and other major compounds of plants, Aageratum spp. 1 10 in combination with an acceptable carrier or diluent can be further prepared into an herbal extract formulations 190 such as, aqueous (liquid), gel, oil or shampoo formulation for topical application.
  • the herbal extract of Ageratum spp. with the above mentioned compounds can be dissolved in the sterile distilled water (at 1-5 g/l) and the aqueous suspension can be filtered (through 1 to 5 ⁇ filter, pH adjusted to neutral) in order to form a liquid formulation 192 of the herbal extract.
  • the herbal extract is dissolved in vegetable oil/PG/PL and the suspension is filtered (through 1 to 5 ⁇ filter) in order to form an oil formulation 194.
  • the herbal extract can be dissolved in a small quantity of ethanol and filtered (through 1 to 5 ⁇ filter) in order to form the gel formulation 96.
  • the mixture can be mixed vigorously using mechanized homogenizer to get the uniform gel product.
  • the herbal extract can be dissolved in an acceptable carrier or diluent in order to get the shampoo formulation 198.
  • the mixture can be mixed vigorously using mechanized homogenizer to get the uniform shampoo product.
  • the herbal extract formulation 190 disclosed herein can be utilized to effectively control the hair loss due to scenarios such as, but not limited to, genetic predisposition, endocrine disorders, medication, radiation, chemotherapy, exposure to chemicals, nutritional factors, generalized or local skin diseases, stress, child birth, Alopecia areata and mechanical damage, Trichotillomania, hair styling treatment, hair braids and weaves.
  • the herbal extract formulation 190 prevents the cease of production of hair due to physiological phase changes such as, Anagen, Catagen and Telogen in humans.
  • FIG. 2 illustrates a high level flow chart of operations illustrating logical operational steps of a method 200 for preparing an herbal extract formulation 190 using herbaceous plants, Ageraium spp. 110, in accordance with the disclosed embodiments.
  • identical or similar blocks are generally indicated by identical reference numerals.
  • the plants, Ageratum spp. 1 10 with 60 to 90 days of age can be identified and the leaves and soft stems of the Ageratum spp. 1 10 can be sliced into pieces (approx. 1 cm size) 120, as illustrated at block 205.
  • 1 10 can be further shade dried at room temperature or dried at 45°C using a fluid flash dryer, as depicted at block 210.
  • a mechanized pulverizing machine 130 can be employed to grind the dried plant slices 20 into coarse powder, as illustrated at block 215.
  • the plant material can be further extracted using the ethanol and methanol solvents and other solvents 160 and 170 separately, as depicted at blocks 220 and 230.
  • the solvent extraction process 140 and 150 described herein can be performed at a 1 :10 (WA/) ratio using a soxhlet apparatus.
  • the green colored solvents can be further extracted and separated by removing ethanol/methanol/other solvents 160 and 170 using a rotoevaporator, as illustrated at blocks 225 and 235.
  • the compounds obtained at both the ethanol solvent extraction process 140 and the methanol solvent extraction process 1 50 can be pooled together or separately in order to form the herbal extract 180 for simulating the hair growth, as depicted at block 240.
  • six major compounds including N,N-bis(trimethylsilyl)-2- phenyl-7-(trifluoromethyl)quinolon-4-amine, S' ⁇ '.S /.S-hexamethoxyflavone, Phytol, Precocene, Caryophyllene, Squalene are identified as common marker components in the hair growth product.
  • the concentrated herbal extract 180 can be further formulated with an acceptable carrier or diluent in order to formulate as liquid, oil, gel or shampoo for topical application.
  • the purified form of herbal extract 180 can be dissolved in the sterile distilled water (at 1-5 g/l) in order to filter the aqueous suspension (through 1 to 5 ⁇ filter, pH adjusted to neutral), as illustrated at block 250.
  • the concentrated material can be further added with acceptable carrier or diluent in order to form the aqueous (liquid) formulation 192 of the herbal extract, as illustrated at block 255.
  • the herbal extract 180 can be dissolved in vegetable oil/PG/PL in order to filter the suspension (through 1 to 5 ⁇ filter), as illustrated at block 260.
  • the concentrated extract 180 can be further added with the acceptable carrier and diluent (e.g., coconut oil) in order to form the oil formulation 94, as depicted at block 265.
  • the concentrated materia! 180 can be dissolved in a small quantity of ethanol in order to filter the suspension (through 1 to 5 ⁇ filter), as illustrated at block 270.
  • the concentrated materia! 180 can be further added with the acceptable carrier and diluent in order to form the gel formulation 196, as depicted at block 275.
  • the mixture can be mixed vigorously using mechanized homogenizer to get the uniform gel product 196.
  • the concentrated material 180 can be further added with the acceptable carrier and diluent in order to form the shampoo formulation 198, as depicted at block 275.
  • the mixture can be mixed vigorously using mechanized homogenizer to get the uniform shampoo product 198.
  • the aqueous (liquid), oil, gel and shampoo formulations 192, 194, 196 and 198 of the herbal extracts are packed separately in PP bottles aseptically.
  • Such an herbal preparation using plants, Ageratum spp. can be utilized as an efficient hair care solution in a wide range of hair growth related problems of humans in a cost effective manner.
  • Group-ll Placebo - 1 (Gel excluding herbal extract)
  • Group-Ill Placebo - 2 (Aqueous excluding herbal extract)
  • Group-IV Placebo - 3 (Oil excluding herbal extract)
  • Group-VI Formulation - 2 (Aqueous with herbal extract)
  • Group I acted as a negative control and Group VIII acted as a positive control.
  • the positive control group received (standard market product) as test compound according to the standard procedures. All the three formulations alone excluding the active principle were kept as Placebo (Group II, HI, and IV). Hair depilation was made by paraffin wax strips. All the test formulations were applied at 24 hours intervals and the animals were kept under observations on changes in skin colour. Since the chosen mice displayed change in the skin colour with reference to hair growth, the indication on skin colour implies the efficacy of the test formulations. It has been observed that both negative control and placebo groups displayed normal hair growth profile, whereas mice in the groups of V, VI, VII and VIII have displayed advancement in hair giowth pattern.
  • the hair density was maximum in Group VIII, followed by V!! and V and the size of hair and hair bulb nature were also significant changed in the above Groups mice compared to negative control and Placebo treated mice.
  • the gel and oil formulations were more effective than the aqueous formulation.
  • the aqueous formulation induced localized hair growth profile, because of non-adherence to the skin, the expected results were not obtained.
  • the agar plates were prepared and the dandruff pathogens, Pityrosporum ovale and Pityrosporum folliculitis were inoculated over the agar surface.
  • Four wells were made using sterile cork borer and 10% aqueous herbal extract of A. conyzoides was ' added at 50 and 100 pi in respective well.
  • Commercial fungicide, Ketaconazole at 100 pg/ml (Positive control) and sterile water (Negative control) were also added in respective wells.
  • the plates were incubated at room temperature for seven days and observed for the inhibition of fungal growth.

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Abstract

L'invention concerne une préparation à base de plantes pour la stimulation de la croissance capillaire et la lutte contre la chute des cheveux, les pellicules et les infections de ceux-ci utilisant des plantes herbacées Ageratum spp. Au moins un composé de plantes, Ageratum spp., tel que, par exemple, la N,N-bis(triméthylsilyl)-2-phényl-7-(trifluorométhyl)quinolon-4-amine, la 3',4',5,6,7,8-hexaméthoxyflavone, le phytol, le précocène, le caryophyllène, le squalène, l'acide α-linolénique, l'acide (Z,Z)-9,12-octadécadiénoïque, l'acide hexadécanoïque et l'hydrocoumarine, peut être extrait à l'aide d'une approche d'extraction de plante commune. L'extraction des composés peut être réalisée à l'aide d'un solvant éthanol et d'un solvant méthanol (à savoir, solvants alcooliques) séparément et les matières concentrées obtenues avec les deux solvants sont rassemblées ou utilisées indépendamment de façon à obtenir un extrait de plante pour stimuler les follicules capillaires. Les composés de plantes Ageratum spp. en combinaison avec un support ou diluant acceptable peuvent en outre être préparés suivant une formulation d'extrait de plante telle qu'une huile ou un gel aqueux (comprenant un shampooing), pour une application topique. Une telle préparation à base de plantes, utilisant des plantes Ageratum spp., peut être utilisée comme solution de soins capillaires efficace dans une large gamme de problèmes associés à la croissance capillaire d'être humains d'une manière économique.
EP13879457.3A 2012-08-16 2013-08-14 Préparation à l'aide de plantes pour la stimulation de la croissance capillaire, la lutte contre la chute des cheveux, les pellicules et les infections de ceux-ci à l'aide d'ageratum spp Withdrawn EP2885054A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN3384CH2012 2012-08-16
PCT/IN2013/000502 WO2014027370A1 (fr) 2012-08-16 2013-08-14 Préparation à l'aide de plantes pour la stimulation de la croissance capillaire, la lutte contre la chute des cheveux, les pellicules et les infections de ceux-ci à l'aide d'ageratum spp.

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EP (1) EP2885054A1 (fr)
KR (1) KR20150042244A (fr)
AU (1) AU2013303762B2 (fr)
CA (1) CA2884774A1 (fr)
NZ (1) NZ705751A (fr)
SG (2) SG10201701217SA (fr)
WO (1) WO2014027370A1 (fr)
ZA (1) ZA201501443B (fr)

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Publication number Priority date Publication date Assignee Title
US9452129B1 (en) * 2015-10-06 2016-09-27 Indfrag Biosciences Inc. Composition of hair oil for stimulation of hair growth, control of hairfall, dandruff and infections thereof
EP3448355B1 (fr) 2016-04-29 2024-05-15 The Procter & Gamble Company Procédé de traitement d'une affection capillaire avec des n-hydroxypyridinones
US9949915B2 (en) 2016-06-10 2018-04-24 Clarity Cosmetics Inc. Non-comedogenic and non-acnegenic hair and scalp care formulations and method for use
US10736398B2 (en) 2016-12-12 2020-08-11 The Procter And Gamble Company Apparatus and method for pulling a strand of hair
CN111423300B (zh) * 2019-01-10 2023-02-28 四川弘合生物科技有限公司 一种β榄香烯的制备方法
EP4013438A4 (fr) * 2019-08-14 2023-09-13 GE Nutrients, Inc. Préparation à base de plantes pour stimuler la pousse des cheveux, lutter contre la chute des cheveux, les pellicules et les infections en relation avec celles-ci, à l'aide d'ageratum spp

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JPH09295919A (ja) * 1996-05-07 1997-11-18 Res Inst For Prod Dev 発毛剤
JP2005008611A (ja) * 2003-06-18 2005-01-13 Yoshimasa Kojima 皮膚外用剤
JP2006062988A (ja) * 2004-08-25 2006-03-09 Mikimoto Pharmaceut Co Ltd エラスターゼ活性阻害剤
JP2006062990A (ja) * 2004-08-25 2006-03-09 Mikimoto Pharmaceut Co Ltd 細胞紫外線損傷回復剤

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Title
See references of WO2014027370A1 *

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SG11201501912PA (en) 2015-05-28
WO2014027370A1 (fr) 2014-02-20
NZ705751A (en) 2018-04-27
AU2013303762A1 (en) 2015-03-19
KR20150042244A (ko) 2015-04-20
AU2013303762B2 (en) 2018-05-31
CA2884774A1 (fr) 2014-02-20
ZA201501443B (en) 2015-12-23
SG10201701217SA (en) 2017-03-30

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