WO2014027370A1 - Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. - Google Patents
Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. Download PDFInfo
- Publication number
- WO2014027370A1 WO2014027370A1 PCT/IN2013/000502 IN2013000502W WO2014027370A1 WO 2014027370 A1 WO2014027370 A1 WO 2014027370A1 IN 2013000502 W IN2013000502 W IN 2013000502W WO 2014027370 A1 WO2014027370 A1 WO 2014027370A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- hair
- herbal
- acid
- herbal extract
- ageratum spp
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/31—Hydrocarbons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/342—Alcohols having more than seven atoms in an unbroken chain
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/368—Carboxylic acids; Salts or anhydrides thereof with carboxyl groups directly bound to carbon atoms of aromatic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/69—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing fluorine
- A61K8/70—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing fluorine containing perfluoro groups, e.g. perfluoroethers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/006—Antidandruff preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
Definitions
- Embodiments are generally related to the field of pharmaceutics, cosmetics and allied industries. Embodiments are also related to herbal extracts for stimulation of hair growth, control of hair fall, dandruff and infections thereof. Embodiments are particularly related to an herbal extraction process for preparing hair growth simulator using herbaceous plants, Ageratum spp. (Ageratum conyzoides (Synonym: Ageratum cperuleum) and Ageratum houstonianum)
- Hair loss/hair fall has become a ubiquitous affliction of human beings (both male and female gender) with the growing impact of global pollution and lifestyle changes.
- Most common reasons of radical or premature hair loss/hair fall are genetic predisposition, endocrine disorders, medication, radiation, chemotherapy, and exposure to chemicals, nutritional factors, generalized or local skin diseases, stress, child birth, Alopecia areata and mechanical damage such as Trichotillomania, hair styling treatment, hair braids and weaves.
- the hair in humans is generated by the hair follicles implanted in the scalp.
- a healthy head of hair is said to contain between 100000 and 150000 hairs, and each hair within this head of hair possesses its own cycle.
- the life cycle of the hair can be. described in three successive physiological phases such as, Anagen, Catagen and Telogen.
- Anagen is a phase of hair growth which may last from a few weeks to 10 years
- Catagen phase is a transient phase of involution of the follicle and ceasing of hair growth with degeneration of the root which may last for a few weeks
- Telogen is a phase of shedding of the hair with the root moving up towards the surface by lasting 1 to 5 months.
- Telogen phase the hair therefore disappears from the scalp and this disappearance may extend from a few days to a few months before the follicle is reactivated to give a new hair in the Anagen phase.
- plugs of skin containing hair are transplanted from areas of the scalp where hair was growing to bald or balding areas of the scalp.
- the transplantation technique can be a time-consuming and relatively costly affair.
- Other approaches include ultra-violet radiation and exercise therapy. While cosmetic aids are another significant approach for the stimulation of hair-growth, there is still a need in the art for compounds which may have greater stimulatory effect on hair growth.
- An herbal preparation for stimulation of hair growth and control of hair fall, dandruff and infections thereof using herbaceous plants, Ageratum spp. (including Ageratum conyzoides, Ageratum coeru!eum and Ageratum houstonianum) is disclosed herein. At least one compound of plants, Ageratum spp.
- CPEA Common Plant Extraction Approach
- the extraction of compounds can be performed utilizing an ethanol solvent and a methanol solvent (i.e., alcoholic solvents) separately and the concentrated materials obtained at both the solvents are pooled together (or separately) in order to obtain an herbal extract for simulating the follicles of the hair.
- the compounds of plants, Ageratum spp. in combination with an acceptable carrier or diluent can be further prepared into an herbal extract formulations such as, a liquid, oil or gel formulation for topical application.
- Such an herbal preparation using plants, Ageratum spp. can be utilized as an efficient hair care solution in a wide range of hair growth related problems of humans in a cost effective manner.
- the Common Plant Extraction Approach (CPEA) described herein can be a principle extraction technique that is well known in the art for extracting the compounds of a plant. Initially, the leaves and soft stems of Ageratum spp. (preferably 60 to 90 days old plants) can be sliced into pieces (approx. 1 cm size) and shade dried at room temperature. The sliced pieces can be also dried using a fluid flash dryer. Further, a mechanized pulverizing machine can be employed to grind the dried plant slices into coarse powder in order to thereby extract the compounds using the ethanol and methanol solvents separately.
- the solvent extraction process described herein can be performed at a 1 :10 (W/V) ratio using a soxhlet apparatus in order to thereby separate the green colored solvent extracts using a rotoevaporator.
- the compounds obtained at both the ethanol solvent extraction process and the methanol solvent extraction process are further pooled together (or used separately) in order to form the herbal extract for simulating the hair growth.
- the compounds extracted at the methanol solvent extraction process can be such as, for example, but not limited to, N ) N-bis(trimethylsilyl)-2-phenyl-7-(trifluoromethyl)quinolon-4-amine, 1 - deoxy-d-mannitol, 15-hydroxypentadecanoic acid, 2-chromenone, 3',4 ⁇ 5, 6,7,8- hexamethoxyflavone, 3,5-di-tert-butylbenzoic acid, 3,7, 1 ,15-tetramethylhexadec-2-eh- 1-ol, 4-tert-butyl-2,6-dimethylacetophenone, 6-vinyl-7-methoxy-2,2-dimethylchromene, Anhydro-d-mannosan, Beta-Funebre
- the compounds extracted at the ethanol solvent extraction process can be such as, for example, but not limited to, N,N-bis(trimethylsilyl)-2-phenyl-7- (trifluoromethyl)quinolon-4-amine, (Z)-,Beta-farnesene, 1-(3,5-ditert-butyl-4- hydroxyphenyl)ethanone, 2-(1 ,3-benzodioxol-5-yl)-5-hydroxy-3,6,7,8-tetramethoxy-4h- chromen-4-one, 2-(3,4-dimethoxyphenyl)-5,6,7,8-tetramethoxy-4H-1 -benzopyran-4-one, 2,4,6-tri-tert-butylphenol, 2-chromanone, 2H-chromen-2-one, 3', 4', 5,6,7, 8- hexamethoxyflavone, 3,5-di-tert-butylbenzoic acid, 3,7, 11
- the solvents utilized for extracting compounds herein should not be construed in any limited sense. Those skilled in the art can understand that similar kind of solvents such as hexane, ethyl acetate, acetone, chloroform, dichloromethane, etc. can also tried for preparing herbal extraction and developing the hair growth stimulant formulation.
- the herbal extract is further formulated with an acceptable carrier or diluent in order to formulate as liquid, shampoo, oil or gel for topical application.
- the .'nerbai extract can be dissolved in the sterile Distilled water (at 1 -5 g/l) and the aqueous suspension can be filtered (through 1 to 5 ⁇ filter, pH adjusted to neutral) in order to form a liquid formulation of the herbal extract.
- the herbal extract is dissolved in vegetable oil or PG/PL and the suspension is filtered (through 1 to 5 ⁇ filter) in order to form an oil formulatidn.
- the herbal extract can be dissolved in a small quantity of ethanol and filtered (through 1 to 5 ⁇ filter) in order to form the gel formulation.
- the mixture can be mixed vigorously using mechanized homogenizer to get the uniform gel product.
- FIG. 1 illustrates a perspective view of a process for preparing an herbal extract formulation using herbaceous plants, Ageratum spp., in accordance with the disclosed embodiments.
- FIG. 2 illustrates a high level flow chart of operations illustrating logical operational steps of a method for preparing herbal extract formulation using herbaceous plants, Ageratum spp., in accordance with the disclosed embodiments.
- FIG. 1 illustrates a perspective view of a process 100 for preparing an herbal extract formulation 190 using herbaceous plants, Ageratum spp. (including Ageratum conyzoides, Synonym: Ageratum coeruleum) and Ageratum houstonianum) .1 10, in accordance with the disclosed embodiments.
- Ageratum spp. are annual herbaceous plants belonging to the family Asteraceae. They are common weeds and grow rapidly. They are known by several names such as goat weed, chick weed, white weed, etc.
- Ageratum spp. are found in many countries ranging from Southeastern North America to Central America, Asia and Africa; but the center of origin is in Central America and the Caribbean. Ageratum spp. are also found in several countries in tropical and subtropical regions, including India, Brazil, Vietnam, Nigeria, Kenya, Congo, Cameroon, etc. in abundance.
- Ageratum spp. are very valuable herbs as they contain many bioactive compounds including flavonoids, alkaloids, cumarins, essential oils, chromenes, benzofurans, terpenoids and tannins that have medicinal, antimicrobial and insecticidal properties.
- the extracts of Ageratum conyzoides 1 10 and other Ageratum species have been used to cure a wide range of ailments such as muscle pains, inflammations, dysentery, indigestion, constipation, wounds in humans.
- the use of Ageratum spp. 1 10 in stimulation of hair follicles is novel. It has not hitherto been known that Ageratum spp. 10 have a simulative action on hair growth and hair fall control.
- a Common Plant Extraction Approach is adapted to prepare the herbal extract from Aageratum spp. 10.
- the Common Plant Extraction Approach (CPEA) described herein can be a principle extraction technique that is well known in the art for extracting the compounds 180 of plants. Note that other extraction processes can be alternatively employed in the place of suggested without losing the scope of invention.
- the leaves and soft stems of Ageratum spp. 0 preferably 60 to 90 days old plants are initially sliced into pieces 120 (i.e., approx. 1 cm) and shade dried at room temperature (approx. 28-30°C) for 48 hrs to 72 hrs.
- The. sliced pieces 120 can be also dried at 45°C using a fluid flash dryer for 24 hrs.
- the dried plant slices 120 are ground to course powder using a mechanized pulverizing machine 130 in order to thereby extract the compounds 180 using the ethanol solvent 160, methanol solvent 170 and other solvents separately.
- the solvent extraction process described herein can be " performed at a 1 :10 (W/V) ratio using a soxhlet apparatus in order thereby separate the green colored solvent extracts using a rotoevaporator.
- the solvents utilized for extracting compounds herein should not be construed in any limited sense. Those skilled in the art can understand that similar kind of solvents such as hexane, ethy! acetate, acetone, chloroform, dichloromethane, etc. can also tried for preparing herbal extraction and developing the hair growth stimulant formulation.
- the compounds extracted at the methanol solvent extraction process 150 can be such as, for example, but not limited to, N,N-bis(trimethylsilyl)-2-phenyl-7- (trifluoromethyl)quinolon-4-amine, -deoxy-d-mannitol, 5-hydroxypentadecanoic acid, 2-chromenone, 3',4',5,6,7,8-hexamethoxyflavone, 3,5-di-tert-butylbenzoic acid, 3,7,1 1 ,15-tetramethylhexadec-2-en-1-ol, 4-tert-butyl-2,6-dimethylacetophenone, 6-vinyl- 7-methoxy-2,2-dimethylchrpmene, Anhydro-d-mannosan, Beta-Funebrene, Caryophyllene, Coumarin, Hexa-o-methylmyricitin, Hydrocoumarin, LinoleoyI chloride, Methyl c
- Table 1 illustrates the list of compounds extracted by treating the plant material 120 using the methanol solvent 170.
- the compounds extracted at the ethanol solvent extraction process can be such as, for example, but not limited to, N,N-bis(trimethylsilyl)-2-phenyl- 7-(trifluoromethyl)quinolon-4-amine, (Z)-,Beta-famesene, 1 -(3,5-ditert-butyl-4- hydroxyphenyl)ethanone, 2-(1 ,3-benzodioxol-5-yl)-5-hydroxy-3,6,7,8-tetramethoxy-4h- chromen-4-one, 2-(3,4-dimethoxyphenyl)-5,6,7,8-tetramethoxy ⁇ 4H-1-benzopyran-4- one, 2,4,6-tri-tert-butylphenol, 2-chromanone, 2H-chromen-2-one, 3', 4', 5,6, 7,8- hexamethoxyflavone, 3,5-di-tert-butylbenzoic acid
- Table 2 illustrates the list of compounds extracted by treating the plant material 120 using the ethanol solvent 170.
- the green colored solvent extract from ethanol/methanol/other solvents are removed using vacuum evaporation.
- Ageratum spp. four compounds, 9,12-octadecadienoic acid (Z,Z)-, Alpha-linolenic acid, Hexadeconoic acid and Hydrocoumarin are identified as 5 Alpha-reductase inhibitors.
- the 5 alpha reductase enzyme metabolizes the testosterone (male hormone) into dihydrotestosterone (DHT), which is known to induce scalp hair loss in man.
- DHT dihydrotestosterone
- the 5 alpha reductase inhibitors present in the extracts of Ageratum spp. can attach with 5 alpha reductase enzyme and prevent it to convert the testosterone into DHT. Thereby decrease and stop the hair loss.
- Table 3 illustrates the list of four compounds identified as 5 Alpha reductase inhibitors present in both ethanol and methanol extracts of the hair growth product.
- Table 3 [0029] The compounds obtained at both the ethanol solvent extraction process 140 and the methanol solvent extraction process 150 (and other solvent processes) are further pooled together in order to form the herbal extract 180 for simulating the hair growth.
- the green colored solvent extracts are separated and the ethanol/methanol/other solvents are removed using vacuum evaporation.
- Table 4 illustrates the list of six major compounds identified as common components present in both ethanol and methanol extracts of the hair growth product.
- the herbal extract contains the above 5 alpha reductase inhibitors and other major compounds of plants, Aageratum spp. 1 10 in combination with an acceptable carrier or diluent can be further prepared into an herbal extract formulations 190 such as, aqueous (liquid), gel, oil or shampoo formulation for topical application.
- the herbal extract of Ageratum spp. with the above mentioned compounds can be dissolved in the sterile distilled water (at 1-5 g/l) and the aqueous suspension can be filtered (through 1 to 5 ⁇ filter, pH adjusted to neutral) in order to form a liquid formulation 192 of the herbal extract.
- the herbal extract is dissolved in vegetable oil/PG/PL and the suspension is filtered (through 1 to 5 ⁇ filter) in order to form an oil formulation 194.
- the herbal extract can be dissolved in a small quantity of ethanol and filtered (through 1 to 5 ⁇ filter) in order to form the gel formulation 96.
- the mixture can be mixed vigorously using mechanized homogenizer to get the uniform gel product.
- the herbal extract can be dissolved in an acceptable carrier or diluent in order to get the shampoo formulation 198.
- the mixture can be mixed vigorously using mechanized homogenizer to get the uniform shampoo product.
- the herbal extract formulation 190 disclosed herein can be utilized to effectively control the hair loss due to scenarios such as, but not limited to, genetic predisposition, endocrine disorders, medication, radiation, chemotherapy, exposure to chemicals, nutritional factors, generalized or local skin diseases, stress, child birth, Alopecia areata and mechanical damage, Trichotillomania, hair styling treatment, hair braids and weaves.
- the herbal extract formulation 190 prevents the cease of production of hair due to physiological phase changes such as, Anagen, Catagen and Telogen in humans.
- FIG. 2 illustrates a high level flow chart of operations illustrating logical operational steps of a method 200 for preparing an herbal extract formulation 190 using herbaceous plants, Ageraium spp. 110, in accordance with the disclosed embodiments.
- identical or similar blocks are generally indicated by identical reference numerals.
- the plants, Ageratum spp. 1 10 with 60 to 90 days of age can be identified and the leaves and soft stems of the Ageratum spp. 1 10 can be sliced into pieces (approx. 1 cm size) 120, as illustrated at block 205.
- 1 10 can be further shade dried at room temperature or dried at 45°C using a fluid flash dryer, as depicted at block 210.
- a mechanized pulverizing machine 130 can be employed to grind the dried plant slices 20 into coarse powder, as illustrated at block 215.
- the plant material can be further extracted using the ethanol and methanol solvents and other solvents 160 and 170 separately, as depicted at blocks 220 and 230.
- the solvent extraction process 140 and 150 described herein can be performed at a 1 :10 (WA/) ratio using a soxhlet apparatus.
- the green colored solvents can be further extracted and separated by removing ethanol/methanol/other solvents 160 and 170 using a rotoevaporator, as illustrated at blocks 225 and 235.
- the compounds obtained at both the ethanol solvent extraction process 140 and the methanol solvent extraction process 1 50 can be pooled together or separately in order to form the herbal extract 180 for simulating the hair growth, as depicted at block 240.
- six major compounds including N,N-bis(trimethylsilyl)-2- phenyl-7-(trifluoromethyl)quinolon-4-amine, S' ⁇ '.S /.S-hexamethoxyflavone, Phytol, Precocene, Caryophyllene, Squalene are identified as common marker components in the hair growth product.
- the concentrated herbal extract 180 can be further formulated with an acceptable carrier or diluent in order to formulate as liquid, oil, gel or shampoo for topical application.
- the purified form of herbal extract 180 can be dissolved in the sterile distilled water (at 1-5 g/l) in order to filter the aqueous suspension (through 1 to 5 ⁇ filter, pH adjusted to neutral), as illustrated at block 250.
- the concentrated material can be further added with acceptable carrier or diluent in order to form the aqueous (liquid) formulation 192 of the herbal extract, as illustrated at block 255.
- the herbal extract 180 can be dissolved in vegetable oil/PG/PL in order to filter the suspension (through 1 to 5 ⁇ filter), as illustrated at block 260.
- the concentrated extract 180 can be further added with the acceptable carrier and diluent (e.g., coconut oil) in order to form the oil formulation 94, as depicted at block 265.
- the concentrated materia! 180 can be dissolved in a small quantity of ethanol in order to filter the suspension (through 1 to 5 ⁇ filter), as illustrated at block 270.
- the concentrated materia! 180 can be further added with the acceptable carrier and diluent in order to form the gel formulation 196, as depicted at block 275.
- the mixture can be mixed vigorously using mechanized homogenizer to get the uniform gel product 196.
- the concentrated material 180 can be further added with the acceptable carrier and diluent in order to form the shampoo formulation 198, as depicted at block 275.
- the mixture can be mixed vigorously using mechanized homogenizer to get the uniform shampoo product 198.
- the aqueous (liquid), oil, gel and shampoo formulations 192, 194, 196 and 198 of the herbal extracts are packed separately in PP bottles aseptically.
- Such an herbal preparation using plants, Ageratum spp. can be utilized as an efficient hair care solution in a wide range of hair growth related problems of humans in a cost effective manner.
- Group-ll Placebo - 1 (Gel excluding herbal extract)
- Group-Ill Placebo - 2 (Aqueous excluding herbal extract)
- Group-IV Placebo - 3 (Oil excluding herbal extract)
- Group-VI Formulation - 2 (Aqueous with herbal extract)
- Group I acted as a negative control and Group VIII acted as a positive control.
- the positive control group received (standard market product) as test compound according to the standard procedures. All the three formulations alone excluding the active principle were kept as Placebo (Group II, HI, and IV). Hair depilation was made by paraffin wax strips. All the test formulations were applied at 24 hours intervals and the animals were kept under observations on changes in skin colour. Since the chosen mice displayed change in the skin colour with reference to hair growth, the indication on skin colour implies the efficacy of the test formulations. It has been observed that both negative control and placebo groups displayed normal hair growth profile, whereas mice in the groups of V, VI, VII and VIII have displayed advancement in hair giowth pattern.
- the hair density was maximum in Group VIII, followed by V!! and V and the size of hair and hair bulb nature were also significant changed in the above Groups mice compared to negative control and Placebo treated mice.
- the gel and oil formulations were more effective than the aqueous formulation.
- the aqueous formulation induced localized hair growth profile, because of non-adherence to the skin, the expected results were not obtained.
- the agar plates were prepared and the dandruff pathogens, Pityrosporum ovale and Pityrosporum folliculitis were inoculated over the agar surface.
- Four wells were made using sterile cork borer and 10% aqueous herbal extract of A. conyzoides was ' added at 50 and 100 pi in respective well.
- Commercial fungicide, Ketaconazole at 100 pg/ml (Positive control) and sterile water (Negative control) were also added in respective wells.
- the plates were incubated at room temperature for seven days and observed for the inhibition of fungal growth.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Emergency Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20157006030A KR20150042244A (en) | 2012-08-16 | 2013-08-14 | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. |
AU2013303762A AU2013303762B2 (en) | 2012-08-16 | 2013-08-14 | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using Ageratum spp. |
CA2884774A CA2884774A1 (en) | 2012-08-16 | 2013-08-14 | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. |
SG11201501912PA SG11201501912PA (en) | 2012-08-16 | 2013-08-14 | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. |
EP13879457.3A EP2885054A1 (en) | 2012-08-16 | 2013-08-14 | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. |
NZ705751A NZ705751A (en) | 2012-08-16 | 2013-08-14 | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. |
ZA2015/01443A ZA201501443B (en) | 2012-08-16 | 2015-03-03 | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IN3384CH2012 | 2012-08-16 | ||
IN3384/CHE/2012 | 2012-08-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2014027370A1 true WO2014027370A1 (en) | 2014-02-20 |
Family
ID=50685444
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IN2013/000502 WO2014027370A1 (en) | 2012-08-16 | 2013-08-14 | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP2885054A1 (en) |
KR (1) | KR20150042244A (en) |
AU (1) | AU2013303762B2 (en) |
CA (1) | CA2884774A1 (en) |
NZ (1) | NZ705751A (en) |
SG (2) | SG10201701217SA (en) |
WO (1) | WO2014027370A1 (en) |
ZA (1) | ZA201501443B (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10159637B2 (en) | 2016-06-10 | 2018-12-25 | Clarity Cosmetics Inc. | Non-comedogenic and non-acnegenic hair and scalp care formulations and method for use |
EP3359125A4 (en) * | 2015-10-06 | 2019-06-12 | Philip Samuel | Composition of hair oil for stimulation of hair growth, control of hairfall, dandruff and infections thereof |
US10543157B2 (en) | 2016-04-29 | 2020-01-28 | The Procter And Gamble Company | Method of treating a hair disorder with N-hydroxypyridinones |
US10736398B2 (en) | 2016-12-12 | 2020-08-11 | The Procter And Gamble Company | Apparatus and method for pulling a strand of hair |
WO2021030717A1 (en) * | 2019-08-14 | 2021-02-18 | Ge Nutrients, Inc. | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111423300B (en) * | 2019-01-10 | 2023-02-28 | 四川弘合生物科技有限公司 | Preparation method of beta elemene |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH09295919A (en) * | 1996-05-07 | 1997-11-18 | Res Inst For Prod Dev | Hair restoring agent |
JP2005008611A (en) * | 2003-06-18 | 2005-01-13 | Yoshimasa Kojima | Skin external preparation |
JP2006062990A (en) * | 2004-08-25 | 2006-03-09 | Mikimoto Pharmaceut Co Ltd | Restoration agent for ultraviolet damage to cell |
JP2006062988A (en) * | 2004-08-25 | 2006-03-09 | Mikimoto Pharmaceut Co Ltd | Elastase activity inhibitor |
-
2013
- 2013-08-14 WO PCT/IN2013/000502 patent/WO2014027370A1/en active Application Filing
- 2013-08-14 SG SG10201701217SA patent/SG10201701217SA/en unknown
- 2013-08-14 SG SG11201501912PA patent/SG11201501912PA/en unknown
- 2013-08-14 EP EP13879457.3A patent/EP2885054A1/en not_active Withdrawn
- 2013-08-14 NZ NZ705751A patent/NZ705751A/en not_active IP Right Cessation
- 2013-08-14 CA CA2884774A patent/CA2884774A1/en not_active Abandoned
- 2013-08-14 AU AU2013303762A patent/AU2013303762B2/en not_active Ceased
- 2013-08-14 KR KR20157006030A patent/KR20150042244A/en not_active Application Discontinuation
-
2015
- 2015-03-03 ZA ZA2015/01443A patent/ZA201501443B/en unknown
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH09295919A (en) * | 1996-05-07 | 1997-11-18 | Res Inst For Prod Dev | Hair restoring agent |
JP2005008611A (en) * | 2003-06-18 | 2005-01-13 | Yoshimasa Kojima | Skin external preparation |
JP2006062990A (en) * | 2004-08-25 | 2006-03-09 | Mikimoto Pharmaceut Co Ltd | Restoration agent for ultraviolet damage to cell |
JP2006062988A (en) * | 2004-08-25 | 2006-03-09 | Mikimoto Pharmaceut Co Ltd | Elastase activity inhibitor |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3359125A4 (en) * | 2015-10-06 | 2019-06-12 | Philip Samuel | Composition of hair oil for stimulation of hair growth, control of hairfall, dandruff and infections thereof |
US10543157B2 (en) | 2016-04-29 | 2020-01-28 | The Procter And Gamble Company | Method of treating a hair disorder with N-hydroxypyridinones |
US10159637B2 (en) | 2016-06-10 | 2018-12-25 | Clarity Cosmetics Inc. | Non-comedogenic and non-acnegenic hair and scalp care formulations and method for use |
US10813872B2 (en) | 2016-06-10 | 2020-10-27 | Clarity Cosmetics Inc. | Hair and scalp formulations |
US11160746B2 (en) | 2016-06-10 | 2021-11-02 | Clarity Cosmetics Inc. | Non-comedogenic and non-acnegenic hair and scalp care formulations and method for use |
US10736398B2 (en) | 2016-12-12 | 2020-08-11 | The Procter And Gamble Company | Apparatus and method for pulling a strand of hair |
WO2021030717A1 (en) * | 2019-08-14 | 2021-02-18 | Ge Nutrients, Inc. | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using ageratum spp. |
CN114555104A (en) * | 2019-08-14 | 2022-05-27 | Ge营养有限公司 | Herbal preparation for stimulating hair growth, controlling hair loss, dandruff and infections thereof using ageratum species |
US11357809B2 (en) | 2019-08-14 | 2022-06-14 | Ge Nutrients, Inc. | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using Ageratum spp |
Also Published As
Publication number | Publication date |
---|---|
CA2884774A1 (en) | 2014-02-20 |
AU2013303762A1 (en) | 2015-03-19 |
KR20150042244A (en) | 2015-04-20 |
EP2885054A1 (en) | 2015-06-24 |
ZA201501443B (en) | 2015-12-23 |
SG10201701217SA (en) | 2017-03-30 |
AU2013303762B2 (en) | 2018-05-31 |
SG11201501912PA (en) | 2015-05-28 |
NZ705751A (en) | 2018-04-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111202708B (en) | Cosmetic composition for improving skin comprising plant cell complex culture | |
AU2013303762B2 (en) | Herbal preparation for stimulation of hair growth, control of hair fall, dandruff and infections thereof using Ageratum spp. | |
KR101549299B1 (en) | Anti-aging and Anti-inflammation and Anti-oxidation cosmetic composition including Lotus Plant Placenta Cell Cultures Extracts | |
KR101533947B1 (en) | Anti-aging and Anti-inflammation and Anti-oxidation cosmetic composition including Paeonia suffruticosa Plant Placenta Cell Cultures Extracts | |
JPH0761915A (en) | External agent for skin | |
WO2005034894A1 (en) | Hair growth stimulant composition | |
KR102032873B1 (en) | Antioxidant and moisturizing cosmetic composition | |
KR20200122304A (en) | Kapok tree flower extract, and cosmetic, pharmaceutical or dermatological composition containing the same | |
KR101561408B1 (en) | Anti-aging and Anti-inflammation and Anti-oxidation cosmetic composition including Peony Plant Placenta Cell Cultures Extracts | |
KR101533213B1 (en) | Anti-aging and Anti-inflammation and Anti-oxidation cosmetic composition including Anemone Plant Placenta Cell Cultures Extracts | |
KR101143717B1 (en) | Composition for Skin External Application Comprising Extract of Nelumbo Nucifera Callus | |
KR101533217B1 (en) | Anti-aging and Anti-inflammation and Anti-oxidation cosmetic composition including Nymphaea tetragona Plant Placenta Cell Cultures Extracts | |
WO2014173635A1 (en) | A topical composition for hair growth | |
KR101173993B1 (en) | An anti-oxidant, anti-inflammatory or anti-allergic composition for cosmetics comprising essentially aplysia kurodai ferments as an effective component and production method of the same | |
KR20100011176A (en) | Natural and oriental herbs complex with anti-hairloss and hair growth stimulating effects | |
KR101793104B1 (en) | Cosmetic composition for whitening skin comprising mixed extract of Cudrania tricuspidata leaf and Morus alba leaf as effective component | |
JP2015193550A (en) | DNA damage inhibitor of hair follicle keratinocyte stem cells | |
KR20070114468A (en) | Hair cosmetic composition containing anti-dandruff materials extracted from ginko and pine leaves | |
KR101852252B1 (en) | Method for culturing Lilium Candidum Cell and Functional skin external application comprising Lilium Candidum Cell Culture Extract | |
JP6655296B2 (en) | Hair growth or hair growth promoter | |
KR102335679B1 (en) | Composition for enhancing skin cell regeneration or wound healing containing absolute of Salix koreensis ANDERSS. flower | |
KR100808418B1 (en) | Cosmetic composition comprising extracts of uncaria gambir roxburgh, cinnamomum zeylanicum nees, sanguisorba officinalis linne, nelumbo nucifera gaertner, pelargonium graveolens with astringent activity | |
KR101533214B1 (en) | Anti-aging and Anti-inflammation and Anti-oxidation cosmetic composition including epatica asiatica Nakai Ranunculales Plant Placenta Cell Cultures Extracts | |
KR101533216B1 (en) | Anti-aging and Anti-inflammation and Anti-oxidation cosmetic composition including Christmas rose Plant Placenta Cell Cultures Extracts | |
KR101533215B1 (en) | Anti-aging and Anti-inflammation and Anti-oxidation cosmetic composition including Pulsatilla koreana Plant Placenta Cell Cultures Extracts |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 13879457 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
ENP | Entry into the national phase |
Ref document number: 20157006030 Country of ref document: KR Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: 2884774 Country of ref document: CA |
|
REEP | Request for entry into the european phase |
Ref document number: 2013879457 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2013879457 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: IDP00201501494 Country of ref document: ID |
|
ENP | Entry into the national phase |
Ref document number: 2013303762 Country of ref document: AU Date of ref document: 20130814 Kind code of ref document: A |