EP2744897A2 - Procédé biotechnologique de synthèse d'acides carboxyliques fonctionnalisés en oméga et esters d'acides carboxyliques à base de sources de carbone simples - Google Patents

Procédé biotechnologique de synthèse d'acides carboxyliques fonctionnalisés en oméga et esters d'acides carboxyliques à base de sources de carbone simples

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Publication number
EP2744897A2
EP2744897A2 EP12745886.7A EP12745886A EP2744897A2 EP 2744897 A2 EP2744897 A2 EP 2744897A2 EP 12745886 A EP12745886 A EP 12745886A EP 2744897 A2 EP2744897 A2 EP 2744897A2
Authority
EP
European Patent Office
Prior art keywords
thioester
acyl
activity
amino
carboxylic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12745886.7A
Other languages
German (de)
English (en)
Inventor
Steffen Schaffer
Nicole Decker
Jasmin GIELEN
Harald HÄGER
Thomas Haas
Markus PÖTTER
Hans-Georg Hennemann
Mirja Wessel
Michael Volland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evonik Operations GmbH
Original Assignee
Evonik Degussa GmbH
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Filing date
Publication date
Application filed by Evonik Degussa GmbH filed Critical Evonik Degussa GmbH
Publication of EP2744897A2 publication Critical patent/EP2744897A2/fr
Withdrawn legal-status Critical Current

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    • C08G69/02—Polyamides derived from amino-carboxylic acids or from polyamines and polycarboxylic acids
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Definitions

  • carbohydrates such as glucose, sucrose, arabinose, xylose, lactose, fructose, maltose, molasses, starch, cellulose and hemicellulose, but also glycerol or simplest organic molecules such as C0 2 , CO or synthesis gas can be used.
  • Alcohol component is derived from methanol, ethanol or other primary alcohols having 3-18 carbon atoms, especially methanol and ethanol.
  • Atopobium Aurantimonas, Azoarcus, Azorhizobium, Azospirillum, Azotobacter, Bacillus,
  • E. coli in particular E. coli, Pseudomonas sp., Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas stutzeri, Acinetobacter sp., Burkholderia sp., Burkholderia thailandensis, Cyanobacteria, Klebsiella sp., Klebsiella oxytoca, Salmonella sp., Rhizobium sp. and
  • This method is also always suitable when possible products of the catalyzed by the enzyme activity to be determined reaction in the microorganism quickly can be metabolized or the activity in the Wiltyp itself is too low to be able to determine the enzyme activity to be determined by the product formation sufficient.
  • the enzyme Ei the reaction of lauric acid and / or lauric acid methyl ester to give co-hydroxy lauric acid and / or co-hydroxy lauric acid methyl ester as a measure of the enzyme activity is understood in particular.
  • preferred P450 alkane hydroxylases are selected from the list AA073954.1, AA073953.1, XP_002546279.1, AAA34353.2, P30607.1, XP_002421627.1, XP_718670.1, CAA39366.1, XP_001527524.1, AA073955.1 , AA073956.1, XP_002546278.1, EEQ43157.1, XP_718669.1, AAA34354.1, P10615.3, XP_002421628.1, 226487, P16141.3, CAA39367.1, Q9Y757.2, XP_001485567.1, AA073958.1 , XP_001383506.2, XP_460111.2, AA073959.1, Q12586.1, XP_460112.2, AAO73960.1, Q12589.1, AA073961.1, XP_460110.2, EEQ43763.1,
  • Microorganisms are known to the person skilled in the art. In particular molecular biological techniques are available here. Instructions for modifying and reducing protein expressions and associated enzyme activity reduction, especially for Candida, in particular for disrupting specific genes, are found in the expert in WO91 / 006660 and WO03 / 100013. Microorganisms preferred according to the invention are characterized in that the
  • ⁇ _08114403.1 ⁇ _003552869.1, ⁇ _002358112.1, ⁇ _08111138.1, ⁇ _003770046.1, ⁇ _003103898.1, ⁇ _08101069.1, ⁇ _08097706.1, ⁇ _08094005.1, ⁇ _003167240.1, ⁇ _002371817.1, ⁇ _004231854.1, EGA98455. 1, ⁇ _002430239.1, ⁇ _01049900.1, ⁇ _769819.1, ⁇ _768378.1, ⁇ _001143837.1, ⁇ _001108475.1, ⁇ _906040.1,
  • YP_294429.1 YP_0010281 12.1, ZP_02479747.1, YP_002874799.1, ZP_03541051 .1,
  • YP_003606536.1 ZP_02887167.1, YP_001795572.1, YP_487451.1, ACZ62814.1,
  • Hexanoic acid synthase a specialized fatty acid synthase of the FAS-I type, which catalyzes the synthesis of hexanoic acid from 2 molecules of malonyl-coenzyme A and one molecule of acetyl-coenzyme A.
  • Fatty acid ethyl esters Fatty acid ethyl esters, fatty alcohols, fatty alkyl acetates, fatty aldehydes, fatty amines, fatty amides, fatty sulfates, fatty ethers, ketones, alkanes, internal and terminal olefins, dicarboxylic acids, ⁇ , ⁇ -dicarboxylic acids and ⁇ , ⁇ -diols, from at least one simple carbon source capable of forming.
  • the document also describes preferred enzymes E according to the invention and their sequences, in particular in sections [0012] to [0060], Tables 7, 17, 26 and 27, FIGS. 1, 44 to 47 and 55 to 59, exemplary embodiments 1 to 38 and claims 1 to 17.
  • WO2010022090 A1 describes, in particular in sections [0022] to [0143] and [0238] to [0275], FIGS. 3 to 5, exemplary embodiment 2 and claims 5, 15, 16 and 36, microorganisms preferably used according to the invention have the first genetic modification, so they more compared to their wild type
  • WO2009140695 A1 describes in particular in the sections [0214] to [0248] and the embodiments 22 to 24 microorganisms which are preferably used according to the invention and have a first genetic engineering modification, so that they contain at least .fat. Fatty acids and fatty acid derivatives, in particular hydrocarbons, compared to their wild type be able to form a simple carbon source.
  • the document also describes preferred enzymes E according to the invention, and their sequences in particular in Table 1, Figure 40 and Examples 22 to 24.
  • microorganisms preferably used according to the invention which have a first genetic modification, so that they are able to form more fatty acids and fatty acid derivatives, in particular fatty acids and fatty acid esters, from at least one simple carbon source compared to their wild type.
  • the document also describes according to the invention preferred enzymes E, and their sequences, in particular on p.193, first paragraph, p.194, first and second paragraph, p.196, second paragraph, and in the Supplementary material.
  • Fatty acid derivatives in particular hexanoic acid from at least one simple carbon source capable of forming.
  • the document also describes preferred enzymes E iv according to the invention and their sequences on, in particular, page 5 and in exemplary embodiment 3.

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Abstract

L'invention concerne un procédé biotechnologique de production d'acides carboxyliques fonctionnalisés et d'esters d'acides carboxyliques fonctionnalisés à base de sources de carbone simples.
EP12745886.7A 2011-08-15 2012-08-15 Procédé biotechnologique de synthèse d'acides carboxyliques fonctionnalisés en oméga et esters d'acides carboxyliques à base de sources de carbone simples Withdrawn EP2744897A2 (fr)

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DE102011110946.7A DE102011110946A1 (de) 2011-08-15 2011-08-15 Biotechnologisches Syntheseverfahren von omegafunktionalisierten Carbonsäuren und Carbonsäure-Estern aus einfachen Kohlenstoffquellen
PCT/EP2012/065941 WO2013024114A2 (fr) 2011-08-15 2012-08-15 Procédé biotechnologique de synthèse d'acides carboxyliques fonctionnalisés en oméga et esters d'acides carboxyliques à base de sources de carbone simples

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