EP2036610A2 - Fixation pour un moyen de support destiné à l'insertion dans une ouverture cylindrique - Google Patents

Fixation pour un moyen de support destiné à l'insertion dans une ouverture cylindrique Download PDF

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Publication number
EP2036610A2
EP2036610A2 EP08015225A EP08015225A EP2036610A2 EP 2036610 A2 EP2036610 A2 EP 2036610A2 EP 08015225 A EP08015225 A EP 08015225A EP 08015225 A EP08015225 A EP 08015225A EP 2036610 A2 EP2036610 A2 EP 2036610A2
Authority
EP
European Patent Office
Prior art keywords
opening
support
holder
web
holder according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP08015225A
Other languages
German (de)
English (en)
Other versions
EP2036610B1 (fr
EP2036610A3 (fr
Inventor
Steven Dr. Ramsey
Florian Bichteler
Wolfgang Dr. Guggenbichler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biocrates Life Sciences AG
Original Assignee
Biocrates Life Sciences AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocrates Life Sciences AG filed Critical Biocrates Life Sciences AG
Publication of EP2036610A2 publication Critical patent/EP2036610A2/fr
Publication of EP2036610A3 publication Critical patent/EP2036610A3/fr
Application granted granted Critical
Publication of EP2036610B1 publication Critical patent/EP2036610B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

Definitions

  • the invention relates to a holder for receiving a carrier, wherein the holder is inserted into a cylindrical opening, for example a multiwell plate. Further, the invention relates to a multiwell plate with such a holder.
  • Such holders are inserted into wells or openings of titration and / or filtration plates and serve to position a carrier means at a defined height above the bottom of the cup.
  • a carrier means at a defined height above the bottom of the cup.
  • Multiwell plates such as 96-well plates, are used in biomedical research, analytical chemistry, and clinical diagnostics as a well-established and widely used standard technique. There is a wide range of modifications to classic multiwell plates. While the classical variant is primarily used for the parallel storage and handling of liquids and reaction solutions, there are variants of multiwell plates for special analytical aspects, such as e.g. the commercially available filtration plate with built-in filter in the bottom for cleaning liquids. There are also more specific variants known as the doppelbödige plate for the cultivation of membrane cell cultures.
  • metabolomics it is attempted to analyze large parts of the metabolome, ie the entirety of all natural metabolites occurring in an organism, as efficiently as possible from very small sample volumes. Several treatment steps on the sample are necessary. It is obvious to make these also in the usual multiwell plate format for the life sciences. For this purpose, it is necessary to introduce additional functionalities in the wells of the multiwell plates. This may be, for example, another floor in the form of a fleece disc, the z. B. may serve as a reaction site, as a reservoir for chemicals or for the immobilization of standards, among others.
  • Multiwell plates have been a standard tool in biomedical and chemical research for many years, either for the simple presentation or storage of liquid volumes in highly parallel fashion, or for the performance of reactions, purifications, extractions, and proofs of substance.
  • the catalogs of relevant manufacturers such as PALL, Millipore, Costar, Nunc, Whatman u.v.a. provide an overview of the diverse areas of application of these panels. more.
  • a carrier When positioning a carrier in a well, for example a filter or fleece disc, it is necessary to prevent the carrier from falling out of the holder or out of the well. Furthermore, some applications require that the carrier can be sufficiently washed by gases or a liquid to be filled into the well.
  • the US 5,208,161 describes a cylindrical holder in which a grid-shaped support is present in the lower region. In the upper part of the cylindrical insert there is a circular ring which is intended to prevent the carrier from slipping out.
  • the EP 0 834 729 A2 describes a multi-part cylindrical insert with a lower part, which has a cylindrical wall, protrude from the inner area support elements with retaining lugs, wherein a filter disc comes to rest on the support elements and fixed by an upper insert which is inserted into the lower insert becomes.
  • the DE 698 31 408 T2 describes a filter plate in which a plurality of filter receiving elements are connected in a matrix, each filter receiving element in its lower region contains support webs between which openings are arranged.
  • the US 4,125,426 shows an insert for a laboratory plate in which a support plate is connected via a rod with a circular handle which rests on the upper edge of an opening of the laboratory plate, wherein the handle serves as a transport aid.
  • the DE 691 09 651 T2 shows a cylindrical cell culture insert in which the upper edge outwardly projecting surfaces are arranged, which come at the upper edge of an opening for abutment.
  • the object of the invention to provide a way for a reliable storage of the carrier in a holder, the holder ensure a secure and defined positioning of the carrier in an opening and reliably prevent falling out of the carrier and the holder from the opening should.
  • the holder according to the invention is an insert for the wells of multiwell plates or microtiter plates, by which the introduction of a carrier, for example.
  • a carrier for example.
  • fleece or filter discs in a defined height above the well bottom is possible.
  • the bracket also prevents slipping out of the carrier and ensures a loose fit of the same. Thereby, and by the distance to the inner side wall of the opening, the flushing with gaseous or liquid substances is made possible.
  • the invention is based on the idea of specifying a holder which is easy to produce and which can easily be inserted into an opening, for example, a Mutliwell plate or a microtiter plate. It is necessary that the holder used always positioned at a precisely defined position in the opening so that the carrier means is in the opening at a well-defined position.
  • the holder of the invention allows not only a manual insertion and an automatic insertion.
  • the holder consists in its basic form of a support and a holding web, wherein between the support and the holding web, a connecting web is arranged. With the connecting web a defined distance between the holding web and the support is ensured, so that the support means between the holding web and the support can be used.
  • the support and / or the holding web or also the connecting web are designed such that lateral outer surfaces of the components of the holder abut against at least one inner side surface of the opening when the holder is inserted into the opening.
  • this defined position can be a defined diameter of the opening or a defined distance to the bottom of the opening.
  • the opening it is possible for the opening to have projections or shoulders on which the holder rests or lies or engages in the inserted state.
  • the support is arranged substantially horizontally, wherein the holding web extends substantially parallel to the support and is also arranged horizontally.
  • the support may be circular or star-shaped.
  • the shape of the retaining bar can be rod-shaped in the simplest variant. It is also possible to form the retaining web U-shaped, V-shaped or circular.
  • a connecting web is provided between support and retaining web.
  • two connecting webs are provided, each of which abuts with its lateral outer surfaces on at least one inner side surface of the opening and thus enable secure positioning, without the holder can tilt or slip in the opening.
  • the support consists of three substantially horizontally extending support webs, which are arranged in a star shape or form a triangle, wherein the support means rests on the support webs.
  • the outwardly projecting ends of the support webs are in the inserted state on or the inner side surfaces of the opening.
  • the outer surfaces of the support webs which abut against the inner side surface of the opening, are bevelled downwards, so that a clamping action between the holder and opening can be achieved can.
  • the slopes or inclinations of the outer surfaces can be adapted to the inclination of the opening.
  • the holder is deposited down to the bottom of the opening.
  • the support has feet, which are preferably positioned at the ends of the support webs. In the embodiment of a circular or triangular support, the positioning of the feet can also be at other locations.
  • the downwardly projecting feet on the support are inclined inwardly or bevelled inwards on their lateral outer surfaces.
  • the at least one connecting web extending between the support or the support webs and the holding web is bent and protrudes in the direction of insertion from above.
  • two connecting webs are provided, abut the lateral outer surfaces respectively on the inner side surface of the opening and provide a stable grip.
  • the holder for secure positioning in the opening at least one fixing web, which protrudes from the holding web upwards and in the inserted state rests against the at least one inner side surface of the opening.
  • the at least one fixing web can preferably be elastically deformable so as to ensure a secure contact with the inner side surface when inserting the holder, in order thus to improve the fixation of the holder.
  • the substantially cylindrical opening may be round or multi-sided.
  • the support preferably abuts at least three points on the inner side surface.
  • the holder abuts each one of the inner side surfaces of the opening.
  • the holder is designed such that the carrier means can be inserted into the opening of the multi-well or microtiter plate before insertion of the holder.
  • the carrier may be a reservoir or a immobilization site for chemicals or serve as a reaction site.
  • the support means may be a filter, which is preferably disc-shaped and consists of a non-woven.
  • the holder is preferably made by injection molding, whereby the design with multiple connecting webs, fixing bars and adapted support webs and support webs is easily possible.
  • the Holder is made of plastic, which is particularly suitable for use in injection molding and is also suitable for use in chemical analysis.
  • the object is further achieved by a multiwell plate, in which a holder described above is used.
  • the holder according to Fig. 1-4 has three horizontal support webs 11, 12, 13, which protrude in a star shape to the outside.
  • the holder 10 shown in the figures is in particular for a circular cylindrical in Fig. 5c shown opening 40 of a multiwell or microtiter plate provided.
  • a holding web 14 is arranged in a U-shape, which is connected via two connecting webs 15, 16 with the support webs 11, 12, 13.
  • the support webs 11, 12, 13 have lateral outer surfaces 11a, 12a and 13a, of which at least one rests against an inner side surface 41 of the opening 40 in the inserted state.
  • the support webs 11, 12, 13 each have an inwardly inclined foot 17, 18, 19, which sit on the bottom of the opening 40 and thus can guarantee a defined distance of the support means 30 from the bottom of the opening 40.
  • two support webs 12 and 13 each project in slightly curved shape connecting webs 15 and 16 from above, between which the holding web 14 extends in a U-shape.
  • the connecting webs 15 and 16 extend in the insertion direction up two Fix istsstege 20 and 21.
  • the outer sides 20 a, 21 a of the fixing webs 20 and 21 are preferably together with the outer sides 15 a, 16 a of the connecting webs 15 and 16 on the inner side surface 41 of Opening 40.
  • FIGS. 5a to 5c the sequence of insertion of the carrier means 30 in the form of a filter or fleece disc in the holder 10 and the insertion of the holder 10 with carrier means 30 in the opening 40, for example, a multiwell plate shown.
  • the filter or nonwoven disk 30 is inserted into the space between the holding web 14 and the support webs 11, 12, 13 prior to insertion of the holder 10 into the opening 40.
  • the holder 10 with the support means 30 is then inserted, for example, with an automatic insertion device in an opening 40 of a multi-well or microtiter plate, wherein some of the outer side surfaces 11a, 12a, 13a, 14a, 15a, 16a, 17a, 18a, 19a, 20a and 21a of the holder 10 ensure a defined position and secure fixation in the opening 40.
  • the holder is not placed on the bottom of an opening, but by means of a Clamping connection between the lateral outer surfaces of the holder and the inner side surface 41 is held firmly in the opening 40.
  • the opening 40 it is possible for the opening 40 to have shoulders or projections on which the holder rests or engages in order to thus ensure a defined position of the holder and of the carrier means inserted in the opening.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
EP08015225.9A 2007-09-13 2008-08-28 Fixation pour un moyen de support destiné à l'insertion dans l'ouverture cylindrique d'une plaque à puits multiples, plaque de titration ou plaque de filtration. Active EP2036610B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102007043614A DE102007043614B3 (de) 2007-09-13 2007-09-13 Halterung für ein Trägermittel zum Einsetzen in eine zylinderförmige Öffnung

Publications (3)

Publication Number Publication Date
EP2036610A2 true EP2036610A2 (fr) 2009-03-18
EP2036610A3 EP2036610A3 (fr) 2012-06-06
EP2036610B1 EP2036610B1 (fr) 2018-11-28

Family

ID=39869081

Family Applications (1)

Application Number Title Priority Date Filing Date
EP08015225.9A Active EP2036610B1 (fr) 2007-09-13 2008-08-28 Fixation pour un moyen de support destiné à l'insertion dans l'ouverture cylindrique d'une plaque à puits multiples, plaque de titration ou plaque de filtration.

Country Status (4)

Country Link
US (1) US8168141B2 (fr)
EP (1) EP2036610B1 (fr)
DE (1) DE102007043614B3 (fr)
ES (1) ES2713037T3 (fr)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4125426A (en) 1975-04-29 1978-11-14 Fujitsu Limited Method of manufacturing semiconductor device
WO1992002303A1 (fr) 1990-08-07 1992-02-20 Pharmacia Lkb Biotechnology Ab Appareil servant a effectuer des reactions biochimiques
US5208161A (en) 1987-08-27 1993-05-04 Polyfiltronics N.A., Inc. Filter units
DE69109651T2 (de) 1990-12-19 1995-09-07 Becton Dickinson Co Zellkultureinsatz.
EP0834729A2 (fr) 1996-09-26 1998-04-08 Becton, Dickinson and Company Plaque à puits multiples et méthode d'analyse pour ADN
WO1999019067A1 (fr) 1997-10-10 1999-04-22 Biosepra, Inc. Pile multicoupelles a plusieurs plaques alignees et procede de traitement d'echantillons biologiques/chimiques de ladite pile
WO2000066268A2 (fr) 1999-05-05 2000-11-09 Millipore Corporation Cupules a dispositif de filtration
WO2002102965A2 (fr) 2001-06-14 2002-12-27 Millipore Corporation Appareil d'analyse a puits multiples
DE69831408T2 (de) 1997-06-06 2006-03-09 Corning Inc. Filterplatte

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4125436A (en) * 1977-08-25 1978-11-14 Linbro Scientific, Inc. Slips for specimen growth and microscopic examination
US5076445A (en) * 1989-08-18 1991-12-31 David Landsberger Circular, collapsible rack for cuvettes and like vessels
US5468638A (en) * 1992-09-28 1995-11-21 Becton, Dickinson And Company Cell culture insert
US6159368A (en) * 1998-10-29 2000-12-12 The Perkin-Elmer Corporation Multi-well microfiltration apparatus
FI20000492A0 (fi) * 2000-03-03 2000-03-03 Labsystems Oy Absorbanssin mittaus
JP2003527953A (ja) * 2000-03-22 2003-09-24 デワルチ テクノロジーズ、インコーポレイテッド 単一容器内での物質の処理方法および器具
US20030226796A1 (en) * 2002-06-11 2003-12-11 3M Innovative Properties Company Modular system for separating components of a liquid sample
JP4478110B2 (ja) * 2003-10-21 2010-06-09 富士フイルム株式会社 核酸分離精製カートリッジの製造方法
JP5155160B2 (ja) * 2005-06-30 2013-02-27 バイオクラテス ライフ サイエンシズ アクチェンゲゼルシャフト 代謝産物特性の定量分析のための装置

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4125426A (en) 1975-04-29 1978-11-14 Fujitsu Limited Method of manufacturing semiconductor device
US5208161A (en) 1987-08-27 1993-05-04 Polyfiltronics N.A., Inc. Filter units
WO1992002303A1 (fr) 1990-08-07 1992-02-20 Pharmacia Lkb Biotechnology Ab Appareil servant a effectuer des reactions biochimiques
DE69109651T2 (de) 1990-12-19 1995-09-07 Becton Dickinson Co Zellkultureinsatz.
EP0834729A2 (fr) 1996-09-26 1998-04-08 Becton, Dickinson and Company Plaque à puits multiples et méthode d'analyse pour ADN
DE69831408T2 (de) 1997-06-06 2006-03-09 Corning Inc. Filterplatte
WO1999019067A1 (fr) 1997-10-10 1999-04-22 Biosepra, Inc. Pile multicoupelles a plusieurs plaques alignees et procede de traitement d'echantillons biologiques/chimiques de ladite pile
WO2000066268A2 (fr) 1999-05-05 2000-11-09 Millipore Corporation Cupules a dispositif de filtration
WO2002102965A2 (fr) 2001-06-14 2002-12-27 Millipore Corporation Appareil d'analyse a puits multiples

Also Published As

Publication number Publication date
US20090072109A1 (en) 2009-03-19
US8168141B2 (en) 2012-05-01
ES2713037T3 (es) 2019-05-17
EP2036610B1 (fr) 2018-11-28
DE102007043614B3 (de) 2008-11-20
EP2036610A3 (fr) 2012-06-06

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