EP1290149A4 - Thermostabile reverse transkriptase und deren anwendungen - Google Patents

Thermostabile reverse transkriptase und deren anwendungen

Info

Publication number
EP1290149A4
EP1290149A4 EP01950251A EP01950251A EP1290149A4 EP 1290149 A4 EP1290149 A4 EP 1290149A4 EP 01950251 A EP01950251 A EP 01950251A EP 01950251 A EP01950251 A EP 01950251A EP 1290149 A4 EP1290149 A4 EP 1290149A4
Authority
EP
European Patent Office
Prior art keywords
reverse transcriptases
thermostable reverse
thermostable
transcriptases
reverse
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP01950251A
Other languages
English (en)
French (fr)
Other versions
EP1290149A1 (de
Inventor
Michael D Smith
Robert Jason Potter
Gulshan Dhariwal
Gary F Gerard
Kim Rosenthal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/808,124 external-priority patent/US7056716B2/en
Priority claimed from US09/845,157 external-priority patent/US7078208B2/en
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Priority to EP16163948.9A priority Critical patent/EP3093341A1/de
Priority to EP10167769.8A priority patent/EP2295551B1/de
Publication of EP1290149A1 publication Critical patent/EP1290149A1/de
Publication of EP1290149A4 publication Critical patent/EP1290149A4/de
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Saccharide Compounds (AREA)
EP01950251A 2000-05-26 2001-05-25 Thermostabile reverse transkriptase und deren anwendungen Ceased EP1290149A4 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP16163948.9A EP3093341A1 (de) 2000-05-26 2001-05-25 Thermostabile reverse transkriptase und deren anwendungen
EP10167769.8A EP2295551B1 (de) 2000-05-26 2001-05-25 Thermostabile reverse Transkriptase und deren Anwendungen

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US808124 1991-12-16
US20719600P 2000-05-26 2000-05-26
US207196P 2000-05-26
US09/808,124 US7056716B2 (en) 2000-03-15 2001-03-15 High fidelity reverse transcriptases and uses thereof
US845157 2001-05-01
US09/845,157 US7078208B2 (en) 2000-05-26 2001-05-01 Thermostable reverse transcriptases and uses thereof
PCT/US2001/016861 WO2001092500A1 (en) 2000-05-26 2001-05-25 Thermostable reverse transcriptases and uses thereof

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP10167769.8A Division EP2295551B1 (de) 2000-05-26 2001-05-25 Thermostabile reverse Transkriptase und deren Anwendungen
EP16163948.9A Division EP3093341A1 (de) 2000-05-26 2001-05-25 Thermostabile reverse transkriptase und deren anwendungen

Publications (2)

Publication Number Publication Date
EP1290149A1 EP1290149A1 (de) 2003-03-12
EP1290149A4 true EP1290149A4 (de) 2004-03-17

Family

ID=27395032

Family Applications (2)

Application Number Title Priority Date Filing Date
EP01950251A Ceased EP1290149A4 (de) 2000-05-26 2001-05-25 Thermostabile reverse transkriptase und deren anwendungen
EP10167769.8A Expired - Lifetime EP2295551B1 (de) 2000-05-26 2001-05-25 Thermostabile reverse Transkriptase und deren Anwendungen

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP10167769.8A Expired - Lifetime EP2295551B1 (de) 2000-05-26 2001-05-25 Thermostabile reverse Transkriptase und deren Anwendungen

Country Status (7)

Country Link
EP (2) EP1290149A4 (de)
JP (1) JP2004511211A (de)
CN (1) CN1430670A (de)
AU (1) AU2001271270A1 (de)
CA (1) CA2406939A1 (de)
NZ (1) NZ522500A (de)
WO (1) WO2001092500A1 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
US9771565B2 (en) 2000-05-26 2017-09-26 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
JP4634799B2 (ja) * 2002-09-13 2011-02-16 ライフ テクノロジーズ コーポレーション 耐熱性逆転写酵素およびその使用法
WO2004068111A2 (en) * 2003-01-29 2004-08-12 Quanta Biosciences, Inc. Methods and compositions for rna detection and quantitation
US7595179B2 (en) 2004-04-19 2009-09-29 Applied Biosystems, Llc Recombinant reverse transcriptases
US9783791B2 (en) 2005-08-10 2017-10-10 Agilent Technologies, Inc. Mutant reverse transcriptase and methods of use
ATE535603T1 (de) * 2005-08-10 2011-12-15 Stratagene California Mutante reverse transkriptase und verwendungsverfahren
JP5279339B2 (ja) 2008-05-16 2013-09-04 タカラバイオ株式会社 逆転写反応用組成物
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
WO2012020759A1 (ja) 2010-08-13 2012-02-16 国立大学法人京都大学 変異型逆転写酵素
KR101818126B1 (ko) * 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
JP2013165669A (ja) * 2012-02-15 2013-08-29 Kyoto Univ 変異型逆転写酵素
JP6221374B2 (ja) * 2012-12-27 2017-11-01 東ソー株式会社 熱安定性が向上したトリ骨髄芽細胞腫ウイルス逆転写酵素
CN106164261A (zh) * 2014-01-22 2016-11-23 生命技术公司 适用于高温核酸合成的新颖逆转录酶
US10364420B2 (en) * 2015-12-07 2019-07-30 Bio-Rad Laboratories, Inc Dimeric reverse transcriptase
CN110291196B (zh) * 2016-12-14 2023-09-12 宝生物工程株式会社 耐热逆转录酶突变体
JP6928668B2 (ja) * 2017-04-11 2021-09-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した熱安定性を有する変異型逆転写酵素、ならびにそれに関する生成物、方法および使用
WO2018200867A1 (en) * 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
CN114174503B (zh) * 2019-07-26 2024-06-28 东洋纺株式会社 稳定性优异的突变型逆转录酶
EP3805380B1 (de) * 2019-10-07 2025-12-03 Qiagen Beverly, LLC Verbesserte thermostabile virale reverse-transkriptase
CN112795546B (zh) * 2019-11-13 2024-02-27 广州达安基因股份有限公司 一种具有高逆转录效率的耐高温逆转录酶突变体及其应用
CN112795551B (zh) * 2019-11-13 2024-01-30 广州达安基因股份有限公司 一种耐高温逆转录酶突变体及其应用
CN111849938B (zh) * 2020-06-05 2022-02-15 广州英赞生物科技有限公司 一种突变型逆转录酶及其制备方法与应用
CN114480335B (zh) * 2020-11-13 2024-06-07 广州达安基因股份有限公司 逆转录酶及逆转录检测试剂
CN114480331A (zh) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 Mmlv酶组合突变体
CN114480333B (zh) * 2020-11-13 2023-12-05 广州达安基因股份有限公司 逆转录酶突变体及其应用
CN114480338B (zh) * 2020-11-13 2024-04-05 广州达安基因股份有限公司 用于核酸检测的逆转录酶突变体
KR102254209B1 (ko) * 2021-01-15 2021-05-18 씨제이제일제당 (주) 신규한 dna 중합효소 ⅲ 감마 및 타우 서브유닛 변이체 및 이를 이용한 l-라이신 생산 방법
CN113234858A (zh) * 2021-05-20 2021-08-10 阿吉安(福州)基因医学检验实验室有限公司 一种covid-19的检测试剂盒
CN113817707B (zh) * 2021-11-23 2022-02-22 中国医学科学院北京协和医院 一种突变重组逆转录酶及其制备方法和应用
CN114317485B (zh) * 2021-12-30 2023-04-11 南京巨匠生物科技有限公司 重组鼠白血病病毒逆转录酶突变体、制备方法及应用
CN114277192B (zh) * 2021-12-30 2022-11-18 南京巨匠生物科技有限公司 用于rt-lamp反应的扩增体系组合物、rna病毒的rt-lamp方法及应用
CN114591930B (zh) * 2022-05-10 2022-08-12 翌圣生物科技(上海)股份有限公司 增强型逆转录酶、编码dna、试剂盒及其在rna文库构建中的应用
CN117586983A (zh) * 2022-08-11 2024-02-23 菲鹏生物股份有限公司 一种逆转录酶、核酸分子及合成cDNA的方法
CN119709683A (zh) * 2024-11-15 2025-03-28 青岛百创智能制造技术有限公司 M-mlv逆转录酶突变体及其制备方法和应用

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US5405776A (en) * 1988-01-13 1995-04-11 Life Technologies, Inc. Cloned genes encoding reverse transcriptase lacking RNase H activity
JP2000139457A (ja) * 1998-11-10 2000-05-23 Toyobo Co Ltd 変異型逆転写酵素
WO2001068895A1 (en) * 2000-03-15 2001-09-20 Invitrogen Corporation High fidelity reverse transcriptases and uses thereof

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US5405776A (en) * 1988-01-13 1995-04-11 Life Technologies, Inc. Cloned genes encoding reverse transcriptase lacking RNase H activity
US5405776B1 (en) * 1988-01-13 1996-10-01 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking RNase H activity
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Title
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HALVAS E K ET AL: "Development of an in vivo assay to identify structural determinants in murine leukemia virus reverse transcriptase important for fidelity", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 74, no. 1, January 2000 (2000-01-01), pages 312 - 319, XP002232188, ISSN: 0022-538X *
KAUSHIK NEERJA ET AL: "Tyrosine 222, a member of the YXDD motif of MuLV RT, is catalytically essential and is a major component of the fidelity center", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 38, no. 9, 2 March 1999 (1999-03-02), pages 2617 - 2627, XP002232187, ISSN: 0006-2960 *
PATENT ABSTRACTS OF JAPAN vol. 2000, no. 08 6 October 2000 (2000-10-06) *
See also references of WO0192500A1 *

Also Published As

Publication number Publication date
EP2295551B1 (de) 2016-04-06
WO2001092500A9 (en) 2003-02-06
JP2004511211A (ja) 2004-04-15
EP2295551A1 (de) 2011-03-16
CN1430670A (zh) 2003-07-16
AU2001271270A1 (en) 2001-12-11
NZ522500A (en) 2004-10-29
WO2001092500A1 (en) 2001-12-06
EP1290149A1 (de) 2003-03-12
CA2406939A1 (en) 2001-12-06

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