EP1290149A4 - Thermostable reverse transcriptases and uses thereof - Google Patents

Thermostable reverse transcriptases and uses thereof

Info

Publication number
EP1290149A4
EP1290149A4 EP01950251A EP01950251A EP1290149A4 EP 1290149 A4 EP1290149 A4 EP 1290149A4 EP 01950251 A EP01950251 A EP 01950251A EP 01950251 A EP01950251 A EP 01950251A EP 1290149 A4 EP1290149 A4 EP 1290149A4
Authority
EP
European Patent Office
Prior art keywords
reverse transcriptases
thermostable reverse
thermostable
transcriptases
reverse
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP01950251A
Other languages
German (de)
French (fr)
Other versions
EP1290149A1 (en
Inventor
Michael D Smith
Robert Jason Potter
Gulshan Dhariwal
Gary F Gerard
Kim Rosenthal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/808,124 external-priority patent/US7056716B2/en
Priority claimed from US09/845,157 external-priority patent/US7078208B2/en
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Priority to EP16163948.9A priority Critical patent/EP3093341A1/en
Priority to EP10167769.8A priority patent/EP2295551B1/en
Publication of EP1290149A1 publication Critical patent/EP1290149A1/en
Publication of EP1290149A4 publication Critical patent/EP1290149A4/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
EP01950251A 2000-05-26 2001-05-25 Thermostable reverse transcriptases and uses thereof Ceased EP1290149A4 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP16163948.9A EP3093341A1 (en) 2000-05-26 2001-05-25 Thermostable reverse transcriptases and uses thereof
EP10167769.8A EP2295551B1 (en) 2000-05-26 2001-05-25 Thermostable reverse transcriptases and uses thereof

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US808124 1991-12-16
US20719600P 2000-05-26 2000-05-26
US207196P 2000-05-26
US09/808,124 US7056716B2 (en) 2000-03-15 2001-03-15 High fidelity reverse transcriptases and uses thereof
US09/845,157 US7078208B2 (en) 2000-05-26 2001-05-01 Thermostable reverse transcriptases and uses thereof
US845157 2001-05-01
PCT/US2001/016861 WO2001092500A1 (en) 2000-05-26 2001-05-25 Thermostable reverse transcriptases and uses thereof

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP16163948.9A Division EP3093341A1 (en) 2000-05-26 2001-05-25 Thermostable reverse transcriptases and uses thereof
EP10167769.8A Division EP2295551B1 (en) 2000-05-26 2001-05-25 Thermostable reverse transcriptases and uses thereof

Publications (2)

Publication Number Publication Date
EP1290149A1 EP1290149A1 (en) 2003-03-12
EP1290149A4 true EP1290149A4 (en) 2004-03-17

Family

ID=27395032

Family Applications (2)

Application Number Title Priority Date Filing Date
EP01950251A Ceased EP1290149A4 (en) 2000-05-26 2001-05-25 Thermostable reverse transcriptases and uses thereof
EP10167769.8A Expired - Lifetime EP2295551B1 (en) 2000-05-26 2001-05-25 Thermostable reverse transcriptases and uses thereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP10167769.8A Expired - Lifetime EP2295551B1 (en) 2000-05-26 2001-05-25 Thermostable reverse transcriptases and uses thereof

Country Status (7)

Country Link
EP (2) EP1290149A4 (en)
JP (1) JP2004511211A (en)
CN (1) CN1430670A (en)
AU (1) AU2001271270A1 (en)
CA (1) CA2406939A1 (en)
NZ (1) NZ522500A (en)
WO (1) WO2001092500A1 (en)

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US7078208B2 (en) 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
US9771565B2 (en) 2000-05-26 2017-09-26 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
JP4634799B2 (en) * 2002-09-13 2011-02-16 ライフ テクノロジーズ コーポレーション Thermostable reverse transcriptase and use thereof
WO2004068111A2 (en) * 2003-01-29 2004-08-12 Quanta Biosciences, Inc. Methods and compositions for rna detection and quantitation
US7595179B2 (en) 2004-04-19 2009-09-29 Applied Biosystems, Llc Recombinant reverse transcriptases
US9783791B2 (en) 2005-08-10 2017-10-10 Agilent Technologies, Inc. Mutant reverse transcriptase and methods of use
JP5203200B2 (en) * 2005-08-10 2013-06-05 アジレント・テクノロジーズ・インク Mutant reverse transcriptase and methods of use
JP5279339B2 (en) 2008-05-16 2013-09-04 タカラバイオ株式会社 Composition for reverse transcription reaction
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
US8900814B2 (en) 2010-08-13 2014-12-02 Kyoto University Variant reverse transcriptase
KR101818126B1 (en) * 2011-02-09 2018-01-15 (주)바이오니아 Reverse Transcriptase Having Improved Thermostability
JP2013165669A (en) * 2012-02-15 2013-08-29 Kyoto Univ Variant reverse transcriptase
JP6221374B2 (en) * 2012-12-27 2017-11-01 東ソー株式会社 Avian myeloblastoma virus reverse transcriptase with improved thermal stability
US9663770B2 (en) * 2014-01-22 2017-05-30 Life Technologies Corporation Reverse transcriptases for use in high temperature nucleic acid synthesis
WO2017100177A1 (en) * 2015-12-07 2017-06-15 Bio-Rad Laboratories, Inc. Dimeric reverse transcriptase
EP3556854A4 (en) * 2016-12-14 2020-08-19 Takara Bio Inc. Heat-resistant reverse transcriptase mutant
EP3610006B1 (en) * 2017-04-11 2021-05-19 Roche Diagnostics GmbH Mutant reverse transcriptase with increased thermal stability as well as products, methods and uses involving the same
WO2018200867A1 (en) * 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
JPWO2021020245A1 (en) * 2019-07-26 2021-02-04
EP3805380A1 (en) * 2019-10-07 2021-04-14 Qiagen Beverly, LLC Improved thermostable viral reverse transcriptase
CN112795551B (en) * 2019-11-13 2024-01-30 广州达安基因股份有限公司 High Wen Ni-resistant transcriptase mutant and application thereof
CN112795546B (en) * 2019-11-13 2024-02-27 广州达安基因股份有限公司 High-temperature-resistant reverse transcriptase mutant with high reverse transcription efficiency and application thereof
CN111849938B (en) * 2020-06-05 2022-02-15 广州英赞生物科技有限公司 Mutant reverse transcriptase and preparation method and application thereof
CN114480333B (en) * 2020-11-13 2023-12-05 广州达安基因股份有限公司 Reverse transcriptase mutant and application thereof
CN114480331A (en) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 MMLV enzyme combination mutants
CN114480338B (en) * 2020-11-13 2024-04-05 广州达安基因股份有限公司 Reverse transcriptase mutants for nucleic acid detection
CN114480335A (en) * 2020-11-13 2022-05-13 广州达安基因股份有限公司 Reverse transcriptase and reverse transcription detection reagent
KR102254209B1 (en) * 2021-01-15 2021-05-18 씨제이제일제당 (주) Novel DNA polymerase Ⅲ subunits gamma and tau variant and a method for producing L-lysine using the same
CN113234858A (en) * 2021-05-20 2021-08-10 阿吉安(福州)基因医学检验实验室有限公司 COVID-19 detection kit
CN113817707B (en) * 2021-11-23 2022-02-22 中国医学科学院北京协和医院 Mutant recombinant reverse transcriptase and preparation method and application thereof
CN114317485B (en) * 2021-12-30 2023-04-11 南京巨匠生物科技有限公司 Recombinant murine leukemia virus reverse transcriptase mutant, preparation method and application
CN114277192B (en) * 2021-12-30 2022-11-18 南京巨匠生物科技有限公司 Amplification system composition for RT-LAMP reaction, RT-LAMP method of RNA virus and application
CN114591930B (en) * 2022-05-10 2022-08-12 翌圣生物科技(上海)股份有限公司 Enhanced reverse transcriptase, coding DNA, kit and application of enhanced reverse transcriptase in construction of RNA library
CN117586983A (en) * 2022-08-11 2024-02-23 菲鹏生物股份有限公司 Reverse transcriptase, nucleic acid molecule and cDNA synthesizing method

Citations (3)

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Publication number Priority date Publication date Assignee Title
US5405776A (en) * 1988-01-13 1995-04-11 Life Technologies, Inc. Cloned genes encoding reverse transcriptase lacking RNase H activity
JP2000139457A (en) * 1998-11-10 2000-05-23 Toyobo Co Ltd Variant type reverse transcriptase
WO2001068895A1 (en) * 2000-03-15 2001-09-20 Invitrogen Corporation High fidelity reverse transcriptases and uses thereof

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WO1987000196A1 (en) 1985-07-09 1987-01-15 Quadrant Bioresources Limited Protection of proteins and the like
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5017492A (en) 1986-02-27 1991-05-21 Life Technologies, Inc. Reverse transcriptase and method for its production
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US4962022A (en) 1986-09-22 1990-10-09 Becton Dickinson And Company Storage and use of liposomes
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5498523A (en) 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
ES2134198T3 (en) 1990-09-28 1999-10-01 Hoffmann La Roche MUTATIONS IN THE 5 'TO 3' EXONUCLEASE OF DNA POLYMERASES.
DE553264T1 (en) 1990-10-05 1994-04-28 Wayne M Barnes THERMOSTABLE DNA POLYMERASE.
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
IL103267A (en) 1991-09-24 2004-07-25 Keygene Nv Process and kit for amplification of restriction fragment obtained from starting dna
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6015668A (en) 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US5948614A (en) 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
EP0942917B1 (en) 1996-08-14 2015-02-25 Life Technologies Corporation Method for nucleic acid amplification and sequencing using stable compositions
WO1998023733A2 (en) * 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
WO1998047912A1 (en) 1997-04-22 1998-10-29 Life Technologies, Inc. Methods for the production of aslv reverse transcriptases composed of multiple subunits
EP0981535A4 (en) 1997-05-12 2000-11-29 Life Technologies Inc Methods for production and purification of nucleic acid molecules
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5405776A (en) * 1988-01-13 1995-04-11 Life Technologies, Inc. Cloned genes encoding reverse transcriptase lacking RNase H activity
US5405776B1 (en) * 1988-01-13 1996-10-01 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking RNase H activity
JP2000139457A (en) * 1998-11-10 2000-05-23 Toyobo Co Ltd Variant type reverse transcriptase
WO2001068895A1 (en) * 2000-03-15 2001-09-20 Invitrogen Corporation High fidelity reverse transcriptases and uses thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GERARD GARY F ET AL: "Reverse transcriptase: The use of cloned Moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA", MOLECULAR BIOTECHNOLOGY, vol. 8, no. 1, 1997, pages 61 - 77, XP001157038, ISSN: 1073-6085 *
HALVAS E K ET AL: "Development of an in vivo assay to identify structural determinants in murine leukemia virus reverse transcriptase important for fidelity", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 74, no. 1, January 2000 (2000-01-01), pages 312 - 319, XP002232188, ISSN: 0022-538X *
KAUSHIK NEERJA ET AL: "Tyrosine 222, a member of the YXDD motif of MuLV RT, is catalytically essential and is a major component of the fidelity center", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 38, no. 9, 2 March 1999 (1999-03-02), pages 2617 - 2627, XP002232187, ISSN: 0006-2960 *
PATENT ABSTRACTS OF JAPAN vol. 2000, no. 08 6 October 2000 (2000-10-06) *
See also references of WO0192500A1 *

Also Published As

Publication number Publication date
EP2295551B1 (en) 2016-04-06
WO2001092500A9 (en) 2003-02-06
NZ522500A (en) 2004-10-29
EP2295551A1 (en) 2011-03-16
CN1430670A (en) 2003-07-16
JP2004511211A (en) 2004-04-15
WO2001092500A1 (en) 2001-12-06
CA2406939A1 (en) 2001-12-06
EP1290149A1 (en) 2003-03-12
AU2001271270A1 (en) 2001-12-11

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Legal Events

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