EP0897517B1 - Procede et dispositif pour congeler des suspensions de cellules - Google Patents

Procede et dispositif pour congeler des suspensions de cellules Download PDF

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Publication number
EP0897517B1
EP0897517B1 EP97923876A EP97923876A EP0897517B1 EP 0897517 B1 EP0897517 B1 EP 0897517B1 EP 97923876 A EP97923876 A EP 97923876A EP 97923876 A EP97923876 A EP 97923876A EP 0897517 B1 EP0897517 B1 EP 0897517B1
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EP
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Prior art keywords
refrigerant
cooling
contact surfaces
chamber
chambers
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EP97923876A
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German (de)
English (en)
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EP0897517A1 (fr
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Bernd Mingers
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    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D31/00—Other cooling or freezing apparatus
    • F25D31/001—Plate freezers
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
    • F25D3/10—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D2400/00—General features of, or devices for refrigerators, cold rooms, ice-boxes, or for cooling or freezing apparatus not covered by any other subclass
    • F25D2400/30—Quick freezing

Definitions

  • the invention relates to a method for freezing cell suspensions as well an apparatus for performing this method.
  • the invention relates in particular to human cell suspensions and will described in more detail below with reference to such substrates, although the invention is also applicable to cell suspensions of other origins.
  • These suspensions contain living cells, which are usually human blood.
  • the aim of the method according to the invention in such embodiments is to freeze such cell suspensions so deeply that on the one hand as possible many vital cells are preserved and on the other hand to avoid their use from preserved blood if necessary to a person's own blood can be used.
  • the flat elastic bag becomes an essentially homogeneous plate shape achieved so that on the one hand a large surface / volume ratio is achieved and on the other hand geometric inhomogeneities such as folds or Bulges are avoided, so that an improved heat transfer is achieved is, but at the same time an uncontrolled cooling by geometric Inhomogeneities of the flat bag can be reliably prevented.
  • the flat bag to be pressed into a thin, homogeneous plate shape frozen content, which in turn saves space and one rapid reheating permitted if necessary.
  • Serve as pressing tools plane-parallel plates, their mutually facing surfaces as cooling surfaces serve that act on the flat sides of the flat container when the press is closed.
  • the plate sides opposite the cooling surfaces act directly after immersing the press in the container liquid refrigerant.
  • a mechanical system of the press ensures that the pressing pressure Maintain during freezing until the flat bag is removed becomes.
  • FR 2 632 391 describes a method for freezing Cell suspensions are known, in which the in a packaging enclosed cell suspensions between contact areas, which are brought to a low temperature, thereby be frozen that the refrigerant packaged the heat Absorbs cell suspensions. From the document mentioned however, no measures are evident by means of which achieved a particularly high and controlled cooling rate could be. In particular, the use of a supercooled Refrigerant not disclosed.
  • an object of the invention is a To provide procedures in which one is simple Way controllable and - if desired - very high cooling rate can be achieved, and this under Use of a supercooled refrigerant.
  • a device for Implementation of the method described above is known with cooling plates through which a refrigerant flows facing sides serve as contact areas and one Limit the gap in each of which is a cell suspension protrudes.
  • the known device has no measures to improve the cooling capacity or the cooling capacity control as well as for easier handling.
  • Another object of the present invention is accordingly therein a device for performing the invention Provide process in which the cooling capacity and their control and manageability improved are.
  • the invention now takes advantage of the fact that in a band of cooling rates a flat maximum of cell recovery between 150 and about 350 K / min (cell recovery) occurs. It takes into account that the cell survival rate at too high cooling rate decreases again.
  • the design is therefore in accordance with the invention the method and a device operating according to this always a special one Cooling process adapted.
  • the frozen goods in which one generally assume can only have one type and size of cell suspensions in bags should be frozen (e.g. either erythrocytes or stem cells or Platelets or leukocytes etc.), which are already at the low for the cooling rate bring brought necessary temperature brought contact surfaces, so that only the individually present frozen food with its content of cell suspension needs to be cooled.
  • the coolant is supercooled and that Process is carried out so that it is the heat from the frozen goods until boiling picks up over the contact areas, bubbles form in the process Refrigerants which, however, collapse again when they rise in the refrigerant, i.e. recondense.
  • the method according to the invention therefore leads to the absorption of heat from the frozen food on the coolant side of the contact surfaces for net vapor formation from the coolant.
  • This new process is compared to the devices that use the flexible frozen food clamp and then immerse in containers with liquid refrigerant, considerably more economical because in particular the repeated cooling and heating the bag chucks and all others with Disadvantages associated with diving the frozen goods are eliminated.
  • the new process also enables optimal cooling rates for a maximum Yield of living cells is a prerequisite. This is a consequence of the high Cooling rate, which is a complete removal of the enthalpy of the frozen food and the Flow in the refrigerant during the cooling process allows through the described bubble formation and bubble recondensation occurs in the refrigerant.
  • the inventive method the heat transfer from the frozen goods on the contact surfaces and from these to the refrigerant with a high cooling rate enables without the cooling according to an exact cooling protocol needs to be carried out, but also within the framework of the procedure
  • the procedure can be carried out at its practical level Usable according to the type of frozen food and according to dimensions of the contact surface to be matched to the cooling rate. Frequently, i.e. especially in the preferably for the invention into consideration upcoming cooling of blood supplies in flexible plastic bags then both the cooling rates and the sizes of the contact areas given. If the method according to the invention is then used, the result is a significantly higher cooling rate than previously.
  • the heat transfer can also in optimize the other direction by changing the thickness of the frozen food between the contact areas increased and thereby higher cooling rates, which the invention enables, for this purpose, the previously experimentally determined cooling rates nevertheless to adhere to or even enlarge.
  • the cooling rate can be increased because this dissipates larger amounts of heat from the frozen goods into the refrigerant become.
  • This can include can be achieved with the features of claim 6.
  • the heat transfer into the freezing agent can also be improved, which among other things. is made possible with the features of claim 7.
  • the process according to the invention is used on an industrial scale, it also offers the advantage of optimal use of the refrigerant.
  • the procedure to be carried out according to claim 5 then in such a way that that of the refrigerant over the contact surfaces Amounts of heat absorbed from the circulating refrigerant be removed by hypothermia before the refrigerant hits the contact surfaces acted upon again.
  • Such, essentially closed The coolant circuit then only needs to replace small amounts of refrigerant, which can arise from inevitable losses in the circuit.
  • the embodiment of the invention is also suitable for the industrial scale Procedure, as this is the heat of the frozen food and the subsequent installation of the contact surfaces on the in flexible packaging located frozen food uses an even, wrinkle-free deformation of the frozen food to a constant, even layer thickness between to reach the contact areas. This is one of the prerequisites for one even deep-freeze with the highest possible proportion living cell substance after thawing.
  • the frozen goods consist of a cell suspension, which is packed in flexible plastic bags 1.
  • These bags are by packaging in a plastic film 2 as packaging material without Loss of their tightness deformable and take on their suspension a movable conveyor of a continuous conveyor 3, as in the illustration above 2 shows a teardrop shape in the vertical cross section, because the cell suspension is liquid. Freezing takes place between contact surfaces of chambers 4, 5, the mode of operation of which is explained in detail below becomes.
  • the two chambers are essentially the same design and are first described in more detail with reference to the details shown in FIG. 4.
  • each chamber 4, 5 is essentially a hollow body 6 semi-cylindrical shape with a rigid outer wall 7, an inner heat-insulating lining 8 and an inner lining 7a.
  • each chamber 4, 5 has a flat inside 9. This is closed with a flat cooling plate 10, 11.
  • Fig. 2 Device serve the opposite outer sides of the cooling plates as contact surfaces 12, 13.
  • each cooling plate 10, 11 is ribbed on the inside.
  • the cooling plates are with the inner molded body walls 7a of the chambers screwed and also point in the rib bases 17 a coating 18, which consists of a microporous material.
  • the heat transfer surfaces are enlarged further their flow of liquid from the chamber inside 9 Degree of heat transfer increased, and also on the coolant side Contact surfaces 12, 13 germs for the bubble boiling of the coolant are available be put.
  • the degree of heat emission are not shown increase in a different way, for example by Surfaces anodized to coolant or color them black.
  • the contact surfaces 12, 13 of the cooling plates 10, 11 are good heat conductors executed.
  • FIG. 1 Further details of the construction of the chamber are shown in FIG. 1 in Connection with FIGS. 2 and 3. Then everyone is in the interior Chamber an intermediate wall 19 which is substantially up to the height of the contact surfaces or the measures on the cooling surfaces sufficient to enlarge of the heat emissivity can be taken.
  • an intermediate wall 19 which is substantially up to the height of the contact surfaces or the measures on the cooling surfaces sufficient to enlarge of the heat emissivity can be taken.
  • a coating 18 made of microporous Material.
  • the partition divides an isolated, the refrigerant supply line 20 in the chamber from a space 21, into which the supercooled coolant flows and the associated contact surfaces 12, 13 at the correspondingly low temperature before touching enters with the plastic bag. If this is the case, a bubble boil sets in a, the schematic in the lower part of FIG. 2 with the gas bubbles formed is shown.
  • the embodiment of FIG. 1 has the advantage that the cooling rate is also controlled by adding heat to the contact surfaces can be used even though a supercooled coolant is used.
  • the contact area 12 can therefore be brought to a higher temperature if necessary.
  • It also has a cooling plate which, like the cooling plate 6 shown, has a comparative character has large mass due to its wall thickness, an additional Heat storage capacity that the temperature increase of the cooling plate during freezing of the refrigerated goods.
  • the chambers 4, 5 and their individual parts described above are part of one Device (Fig. 5) with which flexible plastic bags in large numbers and especially on an industrial scale, rationally using the described method manipulated to freeze them.
  • the manipulator shown serves this purpose 29, the two chambers 4, 5 combined, which are arranged so that their Cooling plates 10, 11 are vertical, so that the contact surfaces 12, 13 in are arranged substantially vertically.
  • the chamber 4 is stationary, while the chamber 5 perpendicular to the contact plate 12 using the piston rod 30 of a double-acting working cylinder and a lever gear in is movable in two positions.
  • a gap opens in the position shown 32, in which the plastic bag to be frozen finds 1 place. In the other Position closes the gap 32.
  • This Position moves the movable chamber 5 after retracting the piston rod 30 in the working cylinder 31.
  • the packaged frozen food already has the required layer thickness when inserted into the gap 32, so that from the Share 30 and 31 existing chamber drive only for creating the Contact surfaces 12, 13 to the frozen goods.
  • the coolant is circulated through the chambers and a cooler 33, which cools the already liquid refrigerant.
  • a pump M promotes the heated Coolant using parallel return lines from the coolant side Compartments 26 and 35 in the cooler 33, from which the supercooled Coolant through parallel feed lines 20 and 36 into the interiors 21 and 37 of the chambers is promoted.
  • the fill level control 24 controls a valve 38, which controls the supply of coolant to compensate for coolant loss.
  • the coolant is controlled by the fill level control 24 by a Valve 38, which the supply of coolant to balance Coolant loss replaced.
  • the coolant vapors pass through parallel Return lines 22 and 39 in a manifold 40, which also is valve-controlled and corresponds functionally to a line 41 which gasifies Coolant discharges from the cooler 33.
  • Hoses as line connections between the two chambers allow their relative movement due to their flexibility perpendicular to the main surfaces of the frozen goods.
  • the embodiment of a two-chamber manipulator 41 differs from the manipulator designed for larger outputs 29 of FIG. 5 by a simplified management of the coolant for a common feed line 20 in the one-piece interior 43 of the fixed Chamber 4 leads, which in turn via its return line 25 initially connected to the movable chamber 5 or its feed line 36 is.
  • the fill level control 24 controls the inflow of the coolant into both Chambers 4, 5, from which only the coolant vapors via the return lines 22 and 39 deducted and replaced by the valve 37 from the feed line become.
  • FIG. 7 shows the two-chamber manipulation 43 in a thermally insulated housing 44, which also the continuous conveyor 3rd completely encloses, only necessary for the cooling chamber circuit Lines 45 for feeding the coolant, 46 for return to the subcooler 33 and out to replace the evaporated coolant at 37 are. In this way an optimal use of energy is achieved.
  • an entrance lock 47 and an exit gate 48 is provided.
  • Locks 49 and 50 are provided while accessing the bicameral manipulator 43 realized by mutually operated sliders 51 and 52 is.
  • the continuous conveyor leads out of the closable with the slides 51 and 52 Interior 53 of the thermally insulated housing 44 into the two lock chambers 47 and 48 and has a rotating conveyor element 54 with a drive and a deflection device 55, 56 and auxiliary guides 57 and 58, which the slip of the conveyor on the drive and deflection devices Avoid 55 and 56 by increasing the wrap angle so that the rotating conveyor can be a rope or chain conveyor on which the Plastic bag 1 can be attached.

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Combustion & Propulsion (AREA)
  • Physics & Mathematics (AREA)
  • Mechanical Engineering (AREA)
  • Thermal Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Devices That Are Associated With Refrigeration Equipment (AREA)

Claims (29)

  1. Procédure pour la congélation de suspensions cellulaires (1), les suspensions cellulaires emballées étant réduites entre les surfaces de contact (12, 13) à la température d'un fluide réfrigérant, étant congelées par l'absorption de la chaleur des suspensions cellulaires emballées par le fluide réfrigérant, la procédure caractérisée en ce qu'un fluide réfrigérant surrefroidi est utilisé qui absorbe la chaleur des suspensions cellulaires emballées (1) n'étant pas encore en ébullition, et la procédure étant gérée de façon que les bulles de fluide réfrigérant qui se produisent lors du commencement de l'ébullition nuclée seront essentiellement recondensées au fluide réfrigérant sans formation de vapeur nette.
  2. Procédure selon la revendication 1, caractérisée en ce que, tout en considérant la capacité calorifique des surfaces de contact, l'épaisseur de la suspension cellulaire emballée entre les surfaces de contact est choisie de manière qu'un taux de refroidissement maximum est causé.
  3. Procédure selon la revendication 1, caractérisée en ce que l'épaisseur des suspensions cellulaires emballées (1) entre les surfaces de contact (12, 13) est adaptée à un taux de refroidissement donné.
  4. Procédure selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que la transmission de chaleur du fluide réfrigérant aux surfaces de contact est optimisée par l'augmentation des surfaces de transmission de chaleur du côté du fluide réfrigérant.
  5. Procédure selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que le fluide réfrigérant est circulé en circulation forcée en fluidité, un surrefroidissement du fluide réfrigérant étant prévu dont la capacité frigorifique est accordée au débit de refoulement de manière que le fluide réfrigérant n'atteigne pas, vers la fin de la procédure de congélation, sa température d'ébullition.
  6. Procédure selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que les surfaces de contact (12, 13) étant pourvues d'une rugosité de la surface petite pour augmenter leur conductivité thermique.
  7. Procédure selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que des germes sont mis à la disposition pour le déclenchement de l'ébullition nuclée à l'aide d'une augmentation de la surface du fluide réfrigérant parmi les surfaces de contact.
  8. Procédure selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que les emballages renfermant la suspension cellulaire sont chambrées, l'une séparément de l'autre, entre les surfaces de contact, réduites à une épaisseur uniforme par pression et libérées après avoir atteint une température minimum spécifiée, les surfaces de contact étant ensuite libérées pour un nouveau processus de congélation.
  9. Dispositif pour la réalisation de la procédure selon l'une ou plusieurs des revendications précédentes muni de deux compartiments (4, 5), dans lesquels circule un fluide réfrigérant et dont les côtés faisant face l'un à l'autre servant comme surfaces de contact (12, 13), limitant une crevasse (32) entre eux, à laquelle saillit une suspension cellulaire emballée (1), caractérisé en ce que le dispositif comprend un manipulateur à deux compartiments (29, 41, 43) les compartiments duquel (4, 5) comprenant des plaques de refroidissement (10, 11) étant arrangées essentiellement verticalement, sur leurs côtés extérieurs façant l'un l'autre, dont les côtés façant l'un l'autre forment les surfaces de contact (12, 13) en limitant la crevasse (32) à laquelle saillit respectivement la suspension cellulaire emballée (1) qui est suspendue au-dessus de la crevasse (32), les compartiments de refroidissement (4, 5) concourant avec un surrefroidisseur (33) du fluide réfrigérant qui refroidit le fluide réfrigérant en dessous de sa température d'ébullition.
  10. Dispositif selon la revendication 9, caractérisé en ce que les plaques de refroidissement (10, 11) limitent les côtés intérieurs (9) des compartiments (4, 5), le fluide réfrigérant refroidi en dessous de sa température d'ébulliton étant alimenté à partir du surrefroidisseur (33) des compartiments (4, 5) à tout près des plaques de refroidissement (10, 11) par une conduite d'alimentation (27, 36) et montant jusqu'à un barrage (19) montant jusqu'au bord supérieur des surfaces de contact (12, 13) de chaque compartiment de refroidissement (4, 5), le fluide réfrigérant descendant à contre-courant en arrière de ce barrage jusqu'à une conduite d'écoulement (25, 34) menant à l'entrée du surrefroidisseur (33).
  11. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que les compartiments de construction identique (4, 5) comprennent une conduite émettant les gaz épurés (29, 39, 40) ainsi qu'une conduite d'alimentation supplémentaire (37) par laquelle du fluide réfrigérant peut être introduit pour compenser les pertes par évaporation.
  12. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisé en ce que les plaques de refroidissement ayant un degré émissif calorifique augmenté à l'aide de nervures métalliques (16) et/ou par un revêtement par une matière microporeuse (18) couvrant au moins une partie des surfaces des plaques de refroidissement (9) affluées par le fluide réfrigérant, ou par anodisation ou noircissement, sur leur côté intérieur du compartiment (9) afflué par le fluide réfrigérant liquide.
  13. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisé en ce que les surfaces de contact (12, 13) des plaques de refroidissement (10, 11) sont munies d'un revêtement lissant, résistant à la corrosion, de bonne conductibilité thermique.
  14. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisé en ce que le manipulateur à deux compartiments (29) est conçu comme une presse à joue mobile pour les suspensions cellulaires souplement emballées (9), dont la crevasse des joues (32) est réglée, en état fermé, à une épaisseur uniforme de la suspension cellulaire emballée.
  15. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisé en ce que les surfaces de contact (12, 13) sont plates ou adaptées, par des courbures sphériques, à la forme des emballages remplis (9) de la suspension cellulaire.
  16. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisé en ce que les surfaces de contact (12, 13) peuvent être chauffées pour contrôler le taux de refroidissement.
  17. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisé en ce que les plaques de refroidissement (10, 11) sont équipées des canalisations (28) conduisant des gaz chauds en circulation pour le but du contrôle du taux de refroidissement.
  18. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisé en ce que les plaques de refroidissement (10,11) sont pourvues d'un chauffage électrique.
  19. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que le manipulateur à deux compartiments (29) est enfermé dans un boítier calorifuge (44) comprenant des sasses (47, 48) pouvant être ouvertes et fermées alternativement pour l'introduction et l'enlèvement des suspensions cellulaires emballées.
  20. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce qu'un engin de manutention continue (3) est pourvu pour l'introduction et l'éclusage.
  21. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce qu'un dispositif chacun pour réduire l'humidité atmosphérique est pourvu dans les compartiments des sasses (47,48), étant synchronisé à l'ouverture et la fermeture des sasses.
  22. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que l'alimentation de l'énergie cinétique au compartiment (4, 5) du manipulateur à deux compartiments (29, 41, 43) effectue une course à vide entre deux processus de congélation successifs, pendant laquelle les surfaces de contact (12, 13) s'approchent l'une à l'autre jusqu'à leur attouchement.
  23. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que les surfaces de contact (12, 13) se réchauffent automatiquement à la température ambiante entre deux processus de congélation successifs.
  24. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce qu'une plaque chauffante à introduire entre les surfaces de contact (12, 13) sert comme contre-chauffage.
  25. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que le manipulateur (29,41,43) comprend un compartiment fixe (4) et un compartiment mobile (5) destiné à ouvrir et fermer la crevasse (32) des surfaces de contact.
  26. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que le circuit du fluide réfrigérant mène à partir d'une conduite d'alimentation commune (45) aux compartiments intérieurs (37) des compartiments (4, 5), passant par des branches de conduite parallèles (20, 36) et par une pompe M passant par les branches de conduite (25, 34) à une conduite (46) menant au surrrefroidisseur (33).
  27. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce que l'alimentation du fluide réfrigérant surrefroidi dans son compartiment (4) s'effectue directement à l'autre compartiment (5), les pertes par évaporation du fluide réfrigérant pouvant être éclusées des compartiments (4, 5) par deux conduites (29, 39).
  28. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisée en ce qu'il est commandé par programme.
  29. Dispositif selon l'une ou plusieurs des revendications précédentes, caractérisé par les séquences de commande par programme suivantes lors de la congélation des suspensions cellulaires emballées en feuille en matière plastique (2) :
    1. L'ouverture de la sasse d'entrée (47) de l'extérieur en appuyant un bouton;
    2. La fixation manuelle de la poche (1) sur l'engin à manutention continue (3);
    3. La fermeture du couvercle (49) de la sasse d'entrée (47) en appuyant un bouton et le démarrage du programme de congélation pour les séquences successives se déroulant automatiquement;
    4. Le séchage de la sasse d'entrée par climatisation pour réduire l'humidité atmosphérique à zéro à l'intérieur de la sasse d'entrée (47);
    5. L'ouverture du coulisseau (52) au manipulateur à deux compartiments (43);
    6. Le transport de la poche de la sasse d'entrée (47);
    7. La fermeture du coulisseau (52) ainsi fermant la sasse d'entrée (47);
    8. L'orientation de la poche (1) entre les plaques de refroidissement (10, 11);
    9. Le serrage des plaques de refroidissement à la pression de serrage préréglée;
    10. La dissipation de chaleur du produit à congéler (refroidissement et congélation de la suspension cellulaire)
    a) en utilisant le courant de chaleur à dissiper en maximum par des réservoirs (7) sur le côté fluide réfrigérant des plaques de refroidissement (10, 11),
    b) en chauffant les plaques de refroidissement par un contre-chauffage lors d'une gestion de la température selon les courbures de refroidissement spécifiées, où nécessaire ;
    11. Le desserrage des plaques de refroidissement (10, 11) lorsque la température minimum préréglée est atteinte;
    12. L'ouverture du coulisseau à la sasse de sortie (48);
    13. Le transport de la poche (1) à la sasse de sortie (48);
    14. La fermeture du coulisseau (51) de la sasse de sortie (48);
    15. L'ouverture du couvercle (50) de la sasse de sortie (48);
    16. L'enlèvement manuel ou mécanique de la poche (1) de la sasse de sortie (48);
    17. La fermeture du couvercle (50) de la sasse de sortie (48);
    18. Le séchage de la sasse de sortie (48) en réduisant à zéro la climatisation de l'humidité atmosphérique à l'intérieur de la sasse de sortie (48).
EP97923876A 1996-05-11 1997-05-12 Procede et dispositif pour congeler des suspensions de cellules Expired - Lifetime EP0897517B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19619152A DE19619152A1 (de) 1996-05-11 1996-05-11 Gefriereinrichtung
DE19619152 1996-05-11
PCT/EP1997/002424 WO1997043586A1 (fr) 1996-05-11 1997-05-12 Procede et dispositif pour congeler des suspensions de cellules

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EP0897517A1 EP0897517A1 (fr) 1999-02-24
EP0897517B1 true EP0897517B1 (fr) 2001-07-25

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EP (1) EP0897517B1 (fr)
AU (1) AU2953597A (fr)
DE (2) DE19619152A1 (fr)
WO (1) WO1997043586A1 (fr)

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DE19736372A1 (de) * 1997-08-21 1999-02-25 Ingo Dipl Ing Heschel Verfahren und Vorrichtung zum Kühlen, insbesondere Gefrieren eines Kühlgutes
US6014864A (en) * 1998-03-16 2000-01-18 Life Science Holdings, Inc. Cryogenic fluid heat exchanger method and apparatus
SE517542C2 (sv) * 1999-09-07 2002-06-18 Ingessons Ingenjoers Ab Fa Frysbox för tillfällig nedfrysning av separata kylelement
DE10129780A1 (de) * 2001-06-20 2003-01-02 Linde Ag Verfahren und Vorrichtung zur Kältebereitstellung
EP1711214A1 (fr) * 2004-02-02 2006-10-18 Interface Multigrad Technology (IMT) Ltd. Dispositif de refroidissement directionnel de matiere biologique
ES2315095B1 (es) * 2006-05-10 2010-01-05 Prendas Capricornio, S.L. Sistema para refrigeracion por contacto.
DE102010007686A1 (de) * 2010-02-08 2011-08-11 Askion GmbH, 07549 Vorrichtung zur Einstellung tiefkalter Temperaturen

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FR1341536A (fr) * 1962-08-02 1963-11-02 Paul Duclos S A Ets Perfectionnements aux installations ou appareils frigorifiques
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Also Published As

Publication number Publication date
EP0897517A1 (fr) 1999-02-24
AU2953597A (en) 1997-12-05
DE59704132D1 (de) 2001-08-30
DE19619152A1 (de) 1997-11-27
WO1997043586A1 (fr) 1997-11-20

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