EP0894532B1 - Method and apparatus for the extraction and handling of biological liquids from thin walled receptacles - Google Patents

Method and apparatus for the extraction and handling of biological liquids from thin walled receptacles Download PDF

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Publication number
EP0894532B1
EP0894532B1 EP98500181A EP98500181A EP0894532B1 EP 0894532 B1 EP0894532 B1 EP 0894532B1 EP 98500181 A EP98500181 A EP 98500181A EP 98500181 A EP98500181 A EP 98500181A EP 0894532 B1 EP0894532 B1 EP 0894532B1
Authority
EP
European Patent Office
Prior art keywords
receptacle
sections
section
thin
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP98500181A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP0894532A3 (en
EP0894532A2 (en
Inventor
Enrique Martinell Gisper-Sauch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Probitas Pharma SA
Original Assignee
Probitas Pharma SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Probitas Pharma SA filed Critical Probitas Pharma SA
Priority to ES98500181T priority Critical patent/ES2227791T3/es
Publication of EP0894532A2 publication Critical patent/EP0894532A2/en
Publication of EP0894532A3 publication Critical patent/EP0894532A3/en
Application granted granted Critical
Publication of EP0894532B1 publication Critical patent/EP0894532B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • B01L3/0296—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids from piercable tubing, e.g. in extracorporeal blood sampling

Definitions

  • the present invention refers to a method and apparatus for handling and extracting biological samples held in receptacles with thin walls such as those obtained by sectioning a tubular element with a number of pockets for the purpose of holding the samples to be analyzed.
  • the present invention offers important characteristics of novelty and inventive activity in relation to the state of the art, enabling biological liquids to be handled in a manner which offers considerable security against external contamination and avoids the possibility of leakage from the sample.
  • the invention is especially applicable to the treatment of blood products, such as whole blood, red cells, serum etc. and facilitates the carrying out of analysis thereof.
  • blood, red cells, serum, etc. from a sample taken from a donor which is intended, for example, for transfusion are usually stored in PVC pockets together with an anti-coagulant and are duly labelled.
  • the contents of the pockets must be analyzed on numerous occasions, at least for each patient who may be given a transfusion, and in these processes it is highly important to prevent accidental contamination (both contamination of the exterior by the contents of the pocket and contamination of the contents from the exterior) and also to prevent confusion occurring in the identification of the samples.
  • each pocket is provided with a long PVC tube which is filled with liquid from the pocket after extraction and is immediately sealed at several points and at its end by welding the walls of the tube by the high frequency method (or any other method).
  • the tube and the pocket can be marked (for example with numbers or bar codes) a number of times so that each segment contains at least one distinguishing mark.
  • the electrodes can leave a notch in the welded joint so that subsequently the segments can be separated from one another by traction leaving both ends completely closed and avoiding any leakage of liquid. This whole process is safe and free of contamination.
  • a test tube is usually taken and placed on a tube rack, and the segment is cut by means of scissors and is emptied by way of the tube, then the empty segment is thrown away and the test is carried out on the tube, which has previously been marked with the same number as that which identifies the pocket.
  • the present invention seeks to overcome the problems described above, by providing a method as defined in claim 1 and an apparatus as defined in claim 2 for extracting and handling blood samples held in container elements with thin walls, enabling the said operations to be carried out safely and aseptically, without any real possibility of actual contact with the analysis personnel and enabling the samples to be identified easily during the whole cycle of tests.
  • the present invention provides apparatus comprising an intermediate receptacle to receive the tubular element holding the sample of biological liquid, the said receptacle being constructed in a manner which is generally similar to that of a test tube, in order to make it possible for the sample to be handled in a manner similar to that which would be used in the case of a test tube, the said intermediate receptacle internally comprising buffering and centring means for the purpose of receiving the tubular receptacle with thin walls for holding the sample of biological liquid, thus creating a lower small container for collecting the biological liquid which will be released from the tubular element which holds it by any suitable means.
  • the size of the intermediate receptacle must be such that, in relation to the tubular element containing the biological sample it enables pipetting to be carried out, possibly by an automatic device of the said intermediate receptacle.
  • the method for handling the biological liquid samples will therefore basically comprise the following successive stages:
  • the device for carrying out this procedure should preferably be manufactured from a low cost plastic material, which is provided internally with means for centring and holding the tubular element holding the biological sample and a small container for collecting the biological liquid after the said tubular element has been ruptured and squeezed, means being provided for rupturing the tubular element and for squeezing out its contents.
  • the intermediate receptacle will be produced from two parts or sections, one of which comprises the centring and holding means for the tubular element which holds the biological sample and likewise, means such as projections, blades or other suitable components for the purpose of making cuts in the tubular element containing the biological sample.
  • one section will preferably be connected to the other, either by being moulded in one piece or by being constructed with external hinges which may be situated in the lower portion of a cover extending lengthwise or laterally.
  • Figures 1 and 2 are both views in cross-section, shown diagrammatically, of one form of device according to the present invention.
  • Figure 3 shows a view from above of a device according to the present invention, which makes it possible to appreciate the internal visibility of the tubular element, due to the fact that it is transparent.
  • Figure 4 shows a diagrammatic cross-section of the device itself.
  • Figures 5 and 6 are views in perspective of a device according to the present invention, showing the components in an open position and in a closed position, respectively.
  • Figure 7 shows a cross-section of a device according to the present invention showing the positioning of a pipetting needle.
  • a cut will be made in one of the said receptacles indicated with the numeral 1 in Figure 1, this being the usual tubular unit containing various individual containers of the same sample; in order to carry out various tests, the said unit is placed centrally inside a special device which basically comprises a section 2 for the purpose of holding the tubular element, a section 3 which can be connected to section 2 and which is designed to compress and rupture at various points of receptacle 1, and a section 4 in the lower portion of which a small container is arranged, this said lower portion being preferably common to sections 2 and 3 and preferably designed to catch the sample of liquid to be analyzed. At least one of the said sections or components 2 and 3 will be constructed from a transparent material, in order to make it possible to read from outside the identification marks of the sample which refer to the receptacle containing the sample.
  • this receptacle being preferably constructed from a thin plastic material
  • the said receptacle will be held in position by means of an abutment 5 which may have various forms, and will be subjected to the action of section 3 of the device, which will in the first place produce transverse compression of the receptacle 1 and will also rupture the walls of the receptacle by contact at several points, one point being preferably situated close to the lower end and another point or points being situated close to the upper portion, in order to permit an intake of air to permit complete emptying of the tube; this will contribute to the compression effect of section 3 upon section 2, thereby squeezing the tube, the contents of which will be conveyed into the lower small container 4 in the form of a liquid mass 6, which will then be available to receive reagents, the said small container being capable of being partially removed it necessary, and also capable of being further handled, all of these operations being carried out by means of a pipetting system as shown
  • sections 2 and 3 of the device will be hinged to each other at the lower section 4.
  • the main characteristic of the device is that the two sections 2 and 3 can be moved in relation to one another, in order to clamp and compress the inner tubular receptacle or container 1, whereby the arrangement of both sections can be variable; for example, they can be movable rectilinearly, in form of laterally hinged container and cover parts, or arranged in any other manner which achieves the required aims, i.e., that the receptacle with thin walls carrying the sample for analysis is well centred, that the liquid contained in the said tubular receptacle is collected, and also that once the integral components of the device have been adapted so as to ensure that the receptacle is ruptured and squeezed, the unit which is constructed in this manner offers fluid-tight conditions which are similar to those of a test tube, and which makes it possible for the device to be used in the later analytical stages.
  • the device which is the subject of the present invention will comprise an internal passage 8 which will be substantially concentric with the liquid mass 6 in the lower small container 4, so as to enable the pipetting needle 7 to be inserted, and similarly the device will be provided with means for cutting the tubular element, the said means being constructed in the form of sharp points or projecting points of another type, knife components, etc. which are shown diagrammatically in the drawings and indicated by the numerals 9, 10 and 11.
  • One of these components will cut into the tubular component near the lower end, which is designed as an outlet for the contents of the receptacle, while the other means, if they are provided, will be situated in the higher portion of the receptacle for the purpose of enabling air to be taken inside the said receptacle, in order to assist the process of emptying the same.
  • the component 2 is provided with projecting points 9, 10 and 11 and the element or section 3 has corresponding recesses 12, 13 and 14 in order to permit the cutting components to be partially inserted into the tubular component 1 for the purpose of rupturing its walls.
  • the described method will comprise a first stage of centring and axially holding the tubular element inside the rupturing and squeezing device, subsequently simultaneously rupturing transversely at a point close to the lower end of the tubular element and optionally at points which are situated in the extreme upper portion of the same and finally compressing the receptacle in order to squeeze out the liquid held inside the same, the empty tubular receptacle becoming lodged inside the device, the said device being manufactured from transparent materials in order to enable the used receptacle to act as a holder for the marks identifying the sample, thus enabling the sample to be identified both visually and automatically in an automated analytical apparatus.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Opening Bottles Or Cans (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
EP98500181A 1997-08-01 1998-07-30 Method and apparatus for the extraction and handling of biological liquids from thin walled receptacles Expired - Lifetime EP0894532B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES98500181T ES2227791T3 (es) 1997-08-01 1998-07-30 Procedimiento y dispositivo para la extraccion y manipulacion de liquidos biologicos contenidos en recipientes de paredes delgadas.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES9701710 1997-08-01
ES009701710A ES2140311B1 (es) 1997-08-01 1997-08-01 "procedimiento y dispositivo para la extraccion y manipulacion de liquidos biologicos contenidos en recipientes de paredes delgadas".

Publications (3)

Publication Number Publication Date
EP0894532A2 EP0894532A2 (en) 1999-02-03
EP0894532A3 EP0894532A3 (en) 2000-07-26
EP0894532B1 true EP0894532B1 (en) 2004-09-22

Family

ID=8300276

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98500181A Expired - Lifetime EP0894532B1 (en) 1997-08-01 1998-07-30 Method and apparatus for the extraction and handling of biological liquids from thin walled receptacles

Country Status (9)

Country Link
EP (1) EP0894532B1 (pt)
JP (1) JP2927784B2 (pt)
AR (1) AR015411A1 (pt)
AT (1) ATE276828T1 (pt)
BR (1) BR9802827B1 (pt)
CZ (1) CZ290694B6 (pt)
DE (1) DE69826385T2 (pt)
ES (2) ES2140311B1 (pt)
SK (1) SK282943B6 (pt)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016011492A1 (en) * 2014-07-24 2016-01-28 Noble House Group Pty. Ltd. Segment sampler

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005094995A1 (de) * 2004-03-31 2005-10-13 Epr Labautomation Ag Verfahren und vorrichtung zum speichern und dispensieren von kleinen flüssigkeitsmengen

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3107009A (en) * 1960-03-21 1963-10-15 Eitel Mccullough Inc Container with cooperating support frame
US4399103A (en) * 1981-01-28 1983-08-16 Ferrara Louis T Blood dispenser device
US4790842A (en) * 1986-09-15 1988-12-13 Coburn Timothy J Blood sample segment detaching and tearing device
US4846005A (en) * 1986-12-12 1989-07-11 Baxter International Inc. Set with attachable sample cell
JPH0755498Y2 (ja) * 1988-07-13 1995-12-20 川澄化学工業株式会社 輸血及び血液検査用の被検血液採取器具

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016011492A1 (en) * 2014-07-24 2016-01-28 Noble House Group Pty. Ltd. Segment sampler

Also Published As

Publication number Publication date
JPH11105985A (ja) 1999-04-20
CZ290694B6 (cs) 2002-09-11
SK101098A3 (en) 1999-02-11
JP2927784B2 (ja) 1999-07-28
ATE276828T1 (de) 2004-10-15
DE69826385D1 (de) 2004-10-28
SK282943B6 (sk) 2003-01-09
AR015411A1 (es) 2001-05-02
BR9802827A (pt) 1999-11-03
DE69826385T2 (de) 2005-09-29
BR9802827B1 (pt) 2010-11-03
ES2227791T3 (es) 2005-04-01
ES2140311B1 (es) 2000-10-16
EP0894532A3 (en) 2000-07-26
CZ229898A3 (cs) 1999-02-17
EP0894532A2 (en) 1999-02-03
ES2140311A1 (es) 2000-02-16

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