EP0848818A1 - Färbung und kapillarobjectglas zum nachweiss von tier- und pflanzenzellen - Google Patents
Färbung und kapillarobjectglas zum nachweiss von tier- und pflanzenzellenInfo
- Publication number
- EP0848818A1 EP0848818A1 EP96929428A EP96929428A EP0848818A1 EP 0848818 A1 EP0848818 A1 EP 0848818A1 EP 96929428 A EP96929428 A EP 96929428A EP 96929428 A EP96929428 A EP 96929428A EP 0848818 A1 EP0848818 A1 EP 0848818A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- slide
- stain
- blood
- base
- cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 11
- 210000004369 blood Anatomy 0.000 claims abstract description 40
- 239000008280 blood Substances 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 238000010186 staining Methods 0.000 claims abstract description 11
- 239000000872 buffer Substances 0.000 claims abstract description 10
- 150000001875 compounds Chemical class 0.000 claims abstract description 8
- 239000012528 membrane Substances 0.000 claims abstract description 8
- 239000003607 modifier Substances 0.000 claims abstract description 8
- 239000002904 solvent Substances 0.000 claims abstract description 8
- 230000002378 acidificating effect Effects 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 21
- 239000000853 adhesive Substances 0.000 claims description 9
- 230000001070 adhesive effect Effects 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 8
- ZNQVEEAIQZEUHB-UHFFFAOYSA-N 2-ethoxyethanol Chemical group CCOCCO ZNQVEEAIQZEUHB-UHFFFAOYSA-N 0.000 claims description 7
- 239000004005 microsphere Substances 0.000 claims description 7
- 239000004033 plastic Substances 0.000 claims description 7
- 229920003023 plastic Polymers 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 230000001086 cytosolic effect Effects 0.000 claims description 5
- 239000000834 fixative Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 229940093475 2-ethoxyethanol Drugs 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 108091092356 cellular DNA Proteins 0.000 claims description 3
- 108091092328 cellular RNA Proteins 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 238000000465 moulding Methods 0.000 claims description 3
- 239000000565 sealant Substances 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical group O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 2
- KFZNPGQYVZZSNV-UHFFFAOYSA-M azure B Chemical class [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(NC)=CC=C3N=C21 KFZNPGQYVZZSNV-UHFFFAOYSA-M 0.000 claims description 2
- 238000001125 extrusion Methods 0.000 claims description 2
- 239000005357 flat glass Substances 0.000 claims description 2
- 239000005329 float glass Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 229920002223 polystyrene Polymers 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 8
- 125000006850 spacer group Chemical group 0.000 description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 239000002356 single layer Substances 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 2
- 229940038773 trisodium citrate Drugs 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Definitions
- red blood cells RBCs
- WBCs white blood cells
- the numbers of each type of cell may be controlled by complex body mechanisms. Any variation from the normal range can indicate serious physiological or pathological changes within the body. Therefore, it is important to be able to determine the number of each type of cell or particle in the blood. This may be achieved by preparation of a suitable sample of the blood or other biological fluid or material of a patient or plant and then screening the sample, for example under a microscope.
- the stain may be used to stain cellular components of plants, and in this specification "plants" should be taken as inclusive of fungi, bacteria and the like present in water and other environmental samples.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9518129 | 1995-09-06 | ||
| GBGB9518129.3A GB9518129D0 (en) | 1995-09-06 | 1995-09-06 | Stain and capillary slide |
| PCT/GB1996/002192 WO1997009616A1 (en) | 1995-09-06 | 1996-09-05 | Stain and capillary slide to detect animal and plant cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP0848818A1 true EP0848818A1 (de) | 1998-06-24 |
Family
ID=10780244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP96929428A Withdrawn EP0848818A1 (de) | 1995-09-06 | 1996-09-05 | Färbung und kapillarobjectglas zum nachweiss von tier- und pflanzenzellen |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0848818A1 (de) |
| JP (1) | JPH11515095A (de) |
| AU (1) | AU6884196A (de) |
| CA (1) | CA2230889A1 (de) |
| GB (1) | GB9518129D0 (de) |
| WO (1) | WO1997009616A1 (de) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9176121B2 (en) | 2004-02-13 | 2015-11-03 | Roche Diagnostics Hematology, Inc. | Identification of blood elements using inverted microscopy |
| CN102687023B (zh) | 2009-11-13 | 2016-01-06 | 文塔纳医疗系统公司 | 用于可调容纳体积的薄膜处理装置 |
| US9498791B2 (en) | 2009-11-13 | 2016-11-22 | Ventana Medical Systems, Inc. | Opposables and automated specimen processing systems with opposables |
| US10746752B2 (en) | 2009-11-13 | 2020-08-18 | Ventana Medical Systems, Inc. | Opposables and automated specimen processing systems with opposables |
| RU2619784C2 (ru) * | 2011-09-13 | 2017-05-18 | Конинклейке Филипс Н.В. | Система и набор для получения цитологических образцов для исследования |
| JP2014002046A (ja) * | 2012-06-19 | 2014-01-09 | Murazumi Kogyo Kk | 液状試料検査用プレパラート |
| USD728120S1 (en) | 2013-03-15 | 2015-04-28 | Ventana Medical Systems, Inc. | Arcuate member for moving liquids along a microscope slide |
| JP2019101021A (ja) * | 2017-11-28 | 2019-06-24 | 東ソー株式会社 | 生体物質保持装置、および生体物質の検出方法 |
-
1995
- 1995-09-06 GB GBGB9518129.3A patent/GB9518129D0/en active Pending
-
1996
- 1996-09-05 JP JP9510980A patent/JPH11515095A/ja active Pending
- 1996-09-05 EP EP96929428A patent/EP0848818A1/de not_active Withdrawn
- 1996-09-05 WO PCT/GB1996/002192 patent/WO1997009616A1/en not_active Application Discontinuation
- 1996-09-05 AU AU68841/96A patent/AU6884196A/en not_active Abandoned
- 1996-09-05 CA CA 2230889 patent/CA2230889A1/en not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| See references of WO9709616A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH11515095A (ja) | 1999-12-21 |
| AU6884196A (en) | 1997-03-27 |
| CA2230889A1 (en) | 1997-03-13 |
| WO1997009616A1 (en) | 1997-03-13 |
| GB9518129D0 (en) | 1995-11-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| 17P | Request for examination filed |
Effective date: 19980406 |
|
| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
| 18D | Application deemed to be withdrawn |
Effective date: 20020403 |